##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1405008
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52128244109642	31.0	31.0	33.0	30.0	34.0
2	31.710550402559985	31.0	31.0	34.0	30.0	34.0
3	31.765737276940772	31.0	31.0	34.0	30.0	34.0
4	35.667153496634896	37.0	35.0	37.0	35.0	37.0
5	27.2733664149955	35.0	26.0	37.0	0.0	37.0
6	31.42202962545409	35.0	28.0	37.0	17.0	37.0
7	14.797332114834933	0.0	0.0	32.0	0.0	37.0
8	24.981023595595186	17.0	17.0	35.0	17.0	37.0
9	33.14764755787867	32.0	32.0	37.0	28.0	39.0
10	36.053617488299	37.0	35.0	39.0	32.0	39.0
11	37.026009816314215	39.0	37.0	39.0	34.0	39.0
12	36.911533599808685	39.0	35.0	39.0	33.0	39.0
13	36.36733456321957	39.0	35.0	39.0	32.0	39.0
14	37.64418280892351	40.0	36.0	41.0	33.0	41.0
15	37.96668773416237	40.0	36.0	41.0	33.0	41.0
16	38.04193285732181	40.0	36.0	41.0	33.0	41.0
17	38.07822944780386	40.0	36.0	41.0	34.0	41.0
18	38.052838133306004	40.0	36.0	41.0	34.0	41.0
19	37.98143355767369	40.0	36.0	41.0	34.0	41.0
20	37.988079783175614	40.0	36.0	41.0	34.0	41.0
21	37.842619401455366	40.0	35.0	41.0	34.0	41.0
22	37.76012022707344	39.0	35.0	41.0	33.0	41.0
23	37.658994824228756	39.0	35.0	41.0	33.0	41.0
24	37.536803349162426	39.0	35.0	41.0	33.0	41.0
25	37.348959578877846	39.0	35.0	41.0	33.0	41.0
26	37.07995114618564	39.0	35.0	40.0	33.0	41.0
27	36.86801285117238	38.0	35.0	40.0	32.0	41.0
28	36.90152013369319	39.0	35.0	40.0	32.0	41.0
29	36.90739696855819	38.0	35.0	40.0	32.0	41.0
30	36.70929062325624	38.0	35.0	40.0	32.0	41.0
31	36.535540722899796	38.0	35.0	40.0	31.0	41.0
32	36.2865777276713	38.0	35.0	40.0	31.0	41.0
33	36.073702783187	38.0	35.0	40.0	30.0	41.0
34	35.8558029562821	38.0	35.0	40.0	30.0	41.0
35	35.71208633687495	38.0	35.0	40.0	29.0	41.0
36	35.48185206062884	38.0	35.0	40.0	29.0	41.0
37	35.28936632389282	38.0	34.0	40.0	27.0	41.0
38	35.150159287349254	38.0	34.0	40.0	27.0	41.0
39	35.10360083358956	38.0	34.0	40.0	27.0	41.0
40	34.874322423786914	37.0	34.0	40.0	26.0	41.0
41	34.73665559199663	37.0	34.0	40.0	26.0	40.0
42	34.73209120517463	37.0	34.0	40.0	26.0	41.0
43	34.587447188912805	37.0	34.0	40.0	25.0	41.0
44	34.380803525673876	37.0	33.0	40.0	24.0	40.0
45	34.150546473756734	36.0	33.0	40.0	24.0	40.0
46	34.14813937002494	36.0	33.0	39.0	24.0	40.0
47	34.02508170771981	36.0	33.0	39.0	24.0	40.0
48	33.96296889412729	36.0	33.0	39.0	24.0	40.0
49	33.84326779633995	36.0	33.0	39.0	24.0	40.0
50	33.90136426269459	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	2.0
14	13.0
15	32.0
16	72.0
17	200.0
18	511.0
19	1078.0
20	1956.0
21	3388.0
22	5261.0
23	7953.0
24	12217.0
25	17727.0
26	22726.0
27	24587.0
28	25541.0
29	28859.0
30	34735.0
31	47321.0
32	66579.0
33	125700.0
34	121087.0
35	145463.0
36	181492.0
37	341194.0
38	153618.0
39	35694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.170996891120907	23.778654641112364	28.265461833669274	16.784886634097457
2	31.30964378850512	24.664201200277862	31.225872023504493	12.800282987712524
3	28.972931114982973	23.251824900641136	35.07809208203796	12.697151902337922
4	26.688317788937855	26.302697208841515	32.64130880393564	14.367676198284993
5	17.129297484427138	48.450329108446354	23.77538063840206	10.644992768724448
6	22.289481625727397	39.023834739731015	28.62631387152244	10.060369763019143
7	38.22867912495872	56.539393370002166	4.235990115358774	0.9959373896803434
8	86.8889002767244	3.456990992222108	8.052267318050859	1.6018414130026308
9	82.6005972919727	5.387442633778598	9.45410986983704	2.5578502044116473
10	54.495917460968194	21.34023436165488	14.407035404780613	9.756812772596312
11	49.390679626023484	18.534200517007733	19.889779987017867	12.18533986995092
12	43.13035940008882	21.017389224830037	23.302714290594785	12.549537084486351
13	21.2326193160466	40.399129399974946	23.768049719289856	14.6002015646886
14	14.626820630202817	42.08438670811839	28.732149567831645	14.556643093847152
15	15.013864689738421	21.805071572546204	49.30178333504151	13.879280402673864
16	15.837062849464203	16.92573992461253	48.50798002573651	18.72921720018676
17	16.529656770637605	18.06018186373316	29.939117784382724	35.47104358124651
18	21.746068349788754	23.61466980970927	33.725288396934396	20.91397344356758
19	30.32267431929213	22.779941466525457	26.073303497204286	20.824080716978123
20	31.457614476216506	22.321225217222963	25.872806418184098	20.348353888376437
21	22.891826950451527	26.49486693314202	28.43713345404439	22.176172662362063
22	22.818019541525743	24.813168323596734	26.073018801316433	26.29579333356109
23	20.645505221322583	29.64025827610946	25.845190917062393	23.869045585505564
24	21.05660608338173	22.73866056278683	38.62298292963457	17.581750424196873
25	18.43021534396957	24.886975732522522	35.917944951203125	20.764863972304784
26	16.932003234145284	33.09817452996709	28.5852464896997	21.384575746187924
27	19.093627936638082	32.1044435334176	30.074846548916444	18.727081981027865
28	16.113787252456927	27.70233336749684	39.11607620739526	17.067803172650976
29	16.393216266384247	25.042704383177888	37.81750708892761	20.746572261510256
30	18.53797273752178	29.38517076059353	31.830352567387514	20.24650393449717
31	28.545531413344268	26.06341031510141	25.834585995239884	19.55647227631444
32	29.982391559336317	25.82995968706228	27.137567899969252	17.05008085363215
33	28.176067324883558	27.565252297495814	25.680280824023775	18.578399553596846
34	19.80529648229761	27.068173277305185	30.1122128841971	23.014317356200106
35	22.23546058100737	24.785125778643252	30.931567649436868	22.047845990912506
36	29.363320351200844	25.481705442246593	26.542055276553583	18.612918929998976
37	21.19916754922392	31.968643594911917	28.675281564233085	18.15690729163108
38	21.558169063806044	32.57831983874825	24.078225889105255	21.78528520834045
39	22.87695159031123	30.279542892282464	26.53693075057224	20.306574766834068
40	23.531467436484345	25.879212075660778	25.536367052714294	25.052953435140584
41	17.766446881441244	25.744622094678466	26.609812897862504	29.87911812601779
42	22.28933927778347	27.297139945110633	23.608904717980252	26.804616059125642
43	23.897159304431007	27.433438101420066	25.064768314486464	23.604634279662466
44	20.95867069796044	31.364447746916746	26.277359274822636	21.399522280300186
45	18.235412182706433	36.03659196246569	22.600440709234395	23.127555145593476
46	21.33703153291654	32.88984831403095	26.518354343889854	19.254765809162652
47	23.641929440971154	26.26938778996276	27.704895630487513	22.38378713857857
48	24.82626433443795	23.273675310033823	29.72217951783904	22.17788083768918
49	20.892977121838452	24.439362622846275	31.082242948082854	23.585417307232415
50	19.192417409722935	32.58992119617824	26.57920808991835	21.638453304180473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	510.0
1	1236.5
2	1963.0
3	8780.0
4	15597.0
5	11893.0
6	8189.0
7	8404.0
8	8619.0
9	8892.5
10	9166.0
11	9106.0
12	9046.0
13	8638.5
14	8231.0
15	7788.5
16	7346.0
17	7055.5
18	6765.0
19	6627.5
20	6490.0
21	6260.5
22	6031.0
23	6281.0
24	6531.0
25	9352.0
26	12173.0
27	15055.5
28	17938.0
29	19915.0
30	21892.0
31	22726.5
32	23561.0
33	27008.0
34	30455.0
35	33587.5
36	36720.0
37	44922.5
38	53125.0
39	70951.5
40	88778.0
41	109624.5
42	130471.0
43	134116.5
44	137762.0
45	130192.0
46	122622.0
47	114299.5
48	105977.0
49	102285.5
50	98594.0
51	94190.0
52	89786.0
53	81982.0
54	74178.0
55	68592.5
56	63007.0
57	58542.5
58	54078.0
59	49919.0
60	45760.0
61	40582.0
62	35404.0
63	29698.0
64	23992.0
65	19868.5
66	15745.0
67	13523.0
68	11301.0
69	9399.0
70	7497.0
71	6060.0
72	4623.0
73	3590.0
74	2557.0
75	1915.0
76	1273.0
77	1031.0
78	789.0
79	572.0
80	355.0
81	211.0
82	67.0
83	47.5
84	28.0
85	17.0
86	6.0
87	6.5
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1405008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.510458817666958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06069374887545	14.079183392817377
2	5.676898294071058	2.101639841689723
3	2.1164397293290387	1.1752881134945812
4	1.2942245627881603	0.9582676188121306
5	0.9304749968433681	0.8611759554968981
6	0.7705210140125269	0.855761849881552
7	0.6438350492530301	0.8342377515198153
8	0.5781410722562098	0.856132520704031
9	0.5290668154331591	0.8813942548982729
>10	8.863035082961217	39.60994975258354
>50	2.197981749520699	28.224397259336264
>100	0.33376151542468163	7.996546502716429
>500	0.002052653846400255	0.2557101490225295
>1k	0.0028737153849603576	1.3103150370268872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4990	0.35515812009611336	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4221	0.30042533565645174	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2119	0.15081764658991265	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2032	0.1446255110291187	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1512	0.10761504560828122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011245487570177536	0.0
2	0.0	0.0	0.0	0.03615637775727967	0.0
3	0.0	0.0	0.0	0.04740186532745721	0.0
4	0.0	0.0	0.0	0.06782879528088097	0.0
5	0.0	0.0	0.0	0.10761504560828124	0.0
6	0.0	0.0	0.0	0.16640474644984227	0.0
7	0.0	0.0	0.0	0.2098920433193263	0.0
8	0.0	0.0	0.0	0.4203534784143578	0.0
9	0.0	0.0	0.0	0.51216790224682	0.0
10	0.0	0.0	0.0	0.6712417295844579	0.0
11	0.0	0.0	0.0	0.8447638732306151	0.0
12	0.0	0.0	0.0	0.9877523829045813	0.0
13	0.0	0.0	0.0	1.0509548700078577	0.0
14	0.0	0.0	0.0	1.070314190381834	0.0
15	0.0	0.0	0.0	1.1072534818307085	0.0
16	0.0	0.0	0.0	1.1888900276724403	0.0
17	0.0	0.0	0.0	1.2900994158040382	0.0
18	0.0	0.0	0.0	1.4383547994032775	0.0
19	0.0	0.0	0.0	1.500276155011217	0.0
20	0.0	0.0	0.0	1.568176124264061	0.0
21	0.0	0.0	0.0	1.6597058522086707	0.0
22	0.0	0.0	0.0	1.7560754102467744	0.0
23	0.0	0.0	0.0	1.8593488435653036	0.0
24	0.0	0.0	0.0	1.9451846537528612	0.0
25	0.0	0.0	0.0	2.006607791557059	0.0
26	0.0	0.0	0.0	2.073582499174382	0.0
27	0.0	0.0	0.0	2.135219158894469	0.0
28	0.0	0.0	0.0	2.2034038240351657	0.0
29	0.0	0.0	0.0	2.2764994932413196	0.0
30	0.0	0.0	0.0	2.3677445252980767	0.0
31	0.0	0.0	0.0	2.4643276052520697	0.0
32	0.0	0.0	0.0	2.5545050277293795	0.0
33	0.0	0.0	0.0	2.637066835206632	0.0
34	0.0	0.0	0.0	2.7245396467493426	0.0
35	0.0	0.0	0.0	2.832866432077255	0.0
36	0.0	0.0	0.0	2.926389031236833	0.0
37	0.0	0.0	0.0	3.0189151947889266	0.0
38	0.0	0.0	0.0	3.103612221425074	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	25	4.443347E-5	44.0	21
CAACGTT	25	4.443347E-5	44.0	35
ACGTTAT	20	7.8570814E-4	44.0	38
CGTGAAT	30	2.5281206E-6	44.0	32
CGCATCG	20	7.8570814E-4	44.0	21
ATTACGG	50	2.7284841E-11	44.0	2
ACTCTAT	20	7.8570814E-4	44.0	19
ATTAACG	30	2.5281206E-6	44.0	1
TTCGTCA	20	7.8570814E-4	44.0	1
TACGCTA	20	7.8570814E-4	44.0	39
GCCGACC	30	2.5281206E-6	44.0	14
CGCTAAT	20	7.8570814E-4	44.0	32
ACTACGG	45	4.802132E-10	44.0	2
ACGTCAA	20	7.8570814E-4	44.0	1
CGTAAGC	20	7.8570814E-4	44.0	31
CGTAAAT	20	7.8570814E-4	44.0	23
CGGTCGA	35	1.4463876E-7	44.0	29
ACGAGTT	20	7.8570814E-4	44.0	10
TAGTCCG	20	7.8570814E-4	44.0	12
TACGTCA	20	7.8570814E-4	44.0	24
>>END_MODULE