FastQCFastQC Report
Sat 18 Jun 2016
SRR3549204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533224
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16800.3150645882405893No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.24061182542421197No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.21473151996159212No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6420.12039968193479664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA302.163304E-645.00000422
GACAACG302.163304E-645.00000412
CGTTATT1950.045.0000041
CGTTAAG650.045.0000042
CGTAAAG351.2102828E-745.0000042
CGTCAAG351.2102828E-745.0000042
TTGGGAC600.045.0000045
ACGCGAT207.02988E-445.026
AACGTAC207.02988E-445.021
TAGCGAA253.8879825E-545.01
TTCACGA207.02988E-445.027
TGTATCG207.02988E-445.013
TCTAGAT207.02988E-445.021
CTGGTCG253.8879825E-545.011
CGTTAGT207.02988E-445.030
AAGCACG207.02988E-445.01
TCGTCCG207.02988E-445.017
GGTATCG207.02988E-445.08
GTCGTAA207.02988E-445.01
TCCGTTC207.02988E-445.015