##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533224
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00629003945809	31.0	31.0	33.0	30.0	33.0
2	31.164152401242255	31.0	31.0	33.0	30.0	34.0
3	31.116483879195236	31.0	31.0	34.0	28.0	34.0
4	35.0863895848649	35.0	35.0	37.0	32.0	37.0
5	32.80645844898204	35.0	35.0	37.0	28.0	37.0
6	33.990210493151096	35.0	35.0	37.0	28.0	37.0
7	14.840761106026735	0.0	0.0	35.0	0.0	37.0
8	24.66492318425277	17.0	17.0	35.0	17.0	37.0
9	32.58827434624098	32.0	32.0	37.0	27.0	39.0
10	35.48478125515731	37.0	34.0	37.0	32.0	39.0
11	36.45377364859796	37.0	35.0	39.0	32.0	39.0
12	36.24422381588226	37.0	35.0	39.0	32.0	39.0
13	35.817757640316266	37.0	35.0	39.0	31.0	39.0
14	36.81997059397177	39.0	35.0	40.0	31.0	41.0
15	37.257597182422394	39.0	35.0	41.0	32.0	41.0
16	37.33313204206863	39.0	35.0	41.0	32.0	41.0
17	37.30035407258488	39.0	35.0	41.0	32.0	41.0
18	37.205740926890016	39.0	35.0	40.0	32.0	41.0
19	37.12971471651689	39.0	35.0	40.0	32.0	41.0
20	37.22023389794908	39.0	35.0	40.0	32.0	41.0
21	37.06184267774894	39.0	35.0	40.0	32.0	41.0
22	37.11226426417416	39.0	35.0	40.0	32.0	41.0
23	37.09270212893643	39.0	35.0	40.0	32.0	41.0
24	36.93721400387079	39.0	35.0	40.0	32.0	41.0
25	36.67090003450707	38.0	35.0	40.0	31.0	41.0
26	36.51615456168515	38.0	35.0	40.0	31.0	41.0
27	36.417876914767525	38.0	35.0	40.0	31.0	41.0
28	36.36707650068264	38.0	35.0	40.0	31.0	41.0
29	36.422514740521805	38.0	35.0	40.0	31.0	41.0
30	36.15786423716862	38.0	35.0	40.0	30.0	41.0
31	36.03385444016023	38.0	35.0	40.0	30.0	41.0
32	35.89706202271466	38.0	34.0	40.0	30.0	41.0
33	35.687407168469534	38.0	34.0	40.0	29.0	41.0
34	35.505903710260604	38.0	34.0	40.0	27.0	41.0
35	35.350331192894544	38.0	34.0	40.0	27.0	41.0
36	35.09482318875369	38.0	34.0	40.0	25.0	41.0
37	34.92821403387695	38.0	34.0	40.0	25.0	41.0
38	34.884181882285866	38.0	34.0	40.0	25.0	41.0
39	34.784788381618235	38.0	34.0	40.0	24.0	41.0
40	34.623030846323495	37.0	33.0	40.0	24.0	41.0
41	34.51615456168515	37.0	33.0	40.0	23.0	41.0
42	34.47549060057312	37.0	33.0	40.0	23.0	41.0
43	34.38081556719128	37.0	33.0	40.0	23.0	41.0
44	34.172105906710875	37.0	33.0	40.0	23.0	41.0
45	34.10633992468456	37.0	33.0	40.0	23.0	41.0
46	34.09998799753949	37.0	33.0	40.0	23.0	41.0
47	34.08048212383539	37.0	33.0	40.0	23.0	41.0
48	34.042850659385174	36.0	33.0	40.0	23.0	41.0
49	33.957713831335425	36.0	33.0	40.0	23.0	41.0
50	33.800174035677315	36.0	33.0	40.0	23.0	41.0
51	33.601195745127754	36.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	0.0
13	2.0
14	9.0
15	23.0
16	65.0
17	151.0
18	416.0
19	751.0
20	1239.0
21	1897.0
22	2845.0
23	4165.0
24	6035.0
25	8145.0
26	9578.0
27	10465.0
28	11226.0
29	13052.0
30	15831.0
31	20463.0
32	27395.0
33	41953.0
34	51771.0
35	52836.0
36	61052.0
37	93508.0
38	83258.0
39	15091.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.582644442110634	24.44563635545287	28.46571047064648	16.50600873179002
2	30.889457338754443	24.4673908151171	31.909478943183352	12.733672902945104
3	29.356705624653053	22.930138178326555	34.86827299596417	12.844883201056218
4	27.26996534289529	26.76679969393726	31.381183142544224	14.58205182062323
5	20.73293775223921	37.455178311553865	28.780399981996307	13.031483954210612
6	22.319888077055797	39.51847628763897	28.27217079501298	9.88946484029226
7	36.55968223485815	58.221122830180185	4.505611150285809	0.7135837846758586
8	87.30814817037492	3.700883681154637	7.431210898234138	1.5597572502362984
9	82.8087257887867	5.60964997824554	8.887446926619958	2.694177306347801
10	54.64420206142259	21.805845198265644	13.906725878805155	9.643226861506609
11	49.91523262268765	18.80766807195475	19.33915202616536	11.937947279192235
12	43.527485634555084	21.37938277346856	22.72590881130632	12.367222780670037
13	21.388759695737626	41.095111997959584	23.103798778749642	14.412329527553148
14	14.427707680074414	43.000502603033624	28.136580499002296	14.435209217889666
15	14.980571017058498	22.174920858776048	49.13976115103596	13.704746973129492
16	15.825244175055886	17.033741917093003	48.443430903335184	18.697583004515923
17	16.38298351161988	18.34538580408984	29.61269560259853	35.658935081691745
18	21.706262283768172	23.941533014267925	33.49230342220155	20.85990127976235
19	30.774683810181084	22.89281802769568	25.667824404002822	20.664673758120415
20	32.050695392555475	22.356270535459768	25.512917648117867	20.080116423866894
21	22.975897558999595	27.071737206127256	27.883778674628303	22.06858656024485
22	22.69327712180997	25.154531678994196	25.471284113243215	26.680907085952622
23	20.50282807975635	30.06297540995904	25.47522242059622	23.958974089688386
24	20.732750213793828	22.990900634630098	38.94817187523442	17.32817727634165
25	18.22048519946589	25.22242059622223	36.029323511319824	20.527770692992064
26	16.714551483054027	33.87994538880471	28.22116033786926	21.184342790272005
27	18.852489760400882	33.274008671777715	29.440722848183874	18.432778719637525
28	15.921638935981877	28.433078781150133	38.82514665506429	16.8201356278037
29	16.21738706434819	25.510854725218667	37.56170014853045	20.71005806190269
30	18.494478868167974	30.339594616896466	31.203209157877364	19.962717357058196
31	28.96174965868003	26.494118794352843	25.451029961142034	19.093101585825096
32	30.702481508709283	25.922126535939867	26.755172310323616	16.62021964502723
33	28.889172280317467	27.76469176181117	25.078578608614766	18.267557349256595
34	19.98090858626018	27.324726568946634	29.376959776754237	23.317405068038948
35	22.29438284848394	24.79295755629904	30.84988672677899	22.06277286843803
36	29.751661590626078	25.855737926274884	26.138920978800655	18.253679504298383
37	21.076320645732373	32.95031731504959	28.20034357043194	17.773018468786102
38	21.229539555608902	33.418038197830555	23.8164448711986	21.535977375361952
39	22.94214063883096	31.036862556824147	26.196682819978097	19.824313984366796
40	23.958599012797624	26.054153601488306	25.235360748953532	24.75188663676054
41	17.684500322566127	26.006893913252217	26.328709885526536	29.97989587865512
42	22.03407948629469	27.239771653188903	23.371978755644907	27.3541701048715
43	23.61409088863217	27.29978395571092	24.956678619116918	24.12944653653999
44	20.599597917573103	31.403687755989978	26.62314524477518	21.373569081661742
45	18.105899209337913	36.82467405818193	22.322701153736517	22.746725578743643
46	21.23816632409644	33.80286708775299	25.842985311988958	19.115981276161616
47	23.397108907326004	26.539315559689737	27.383801179241747	22.67977435374252
48	24.416005281082622	23.744430108172175	29.969956341049915	21.869608269695288
49	20.930603273671103	25.082704454413157	31.148635470271408	22.83805680164434
50	19.094226816497383	33.60857725833796	26.183930205692167	21.113265719472494
51	18.841612530568767	33.72110032556674	23.565518431278413	23.871768712586082
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	471.0
2	697.0
3	2811.5
4	4926.0
5	3853.5
6	2781.0
7	2845.5
8	2910.0
9	3032.0
10	3154.0
11	3042.0
12	2930.0
13	2836.0
14	2742.0
15	2656.0
16	2570.0
17	2487.5
18	2405.0
19	2367.0
20	2329.0
21	2380.5
22	2432.0
23	2416.5
24	2401.0
25	3099.5
26	4849.0
27	5900.0
28	6942.5
29	7985.0
30	8744.0
31	9503.0
32	10345.5
33	11188.0
34	12487.5
35	13787.0
36	15902.5
37	18018.0
38	23823.5
39	29629.0
40	37031.5
41	44434.0
42	48252.5
43	52071.0
44	49808.5
45	47546.0
46	44828.0
47	42110.0
48	40045.5
49	37981.0
50	36229.0
51	34477.0
52	31861.0
53	29245.0
54	27145.5
55	25046.0
56	23801.0
57	22556.0
58	20588.0
59	18620.0
60	16631.0
61	14642.0
62	12921.5
63	11201.0
64	9276.5
65	7352.0
66	5980.0
67	4608.0
68	4072.0
69	3536.0
70	2987.0
71	2438.0
72	1897.5
73	1357.0
74	1063.0
75	601.0
76	433.0
77	351.0
78	269.0
79	202.0
80	135.0
81	85.0
82	35.0
83	24.0
84	13.0
85	10.0
86	7.0
87	7.5
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.62930854833705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.14289312653388	19.211153606361748
2	6.027544187133366	3.210186678958197
3	3.0637904991331215	2.4475986758663844
4	2.137408869129949	2.2767088108005438
5	1.6826925940796784	2.2404470139874713
6	1.3860548616012505	2.2145809544701383
7	1.1237251796746304	2.094681717316386
8	1.0562034634160504	2.2500774333702585
9	0.8894853787470516	2.131774253990071
>10	10.231041265864116	56.21185297593573
>50	0.23820419398444975	3.9203288426671765
>100	0.018162196608785024	0.9070236506213594
>500	6.985460234148086E-4	0.11942354054530614
>1k	0.0020956380702444257	0.7641618451092175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1680	0.3150645882405893	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1283	0.24061182542421197	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1145	0.21473151996159212	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	642	0.12039968193479664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009939537605209069	0.0
2	0.0	0.0	0.0	0.03506968928630369	0.0
3	0.0	0.0	0.0	0.04238368865617451	0.0
4	0.0	0.0	0.0	0.058887071849729196	0.0
5	0.0	0.0	0.0	0.09208137668221986	0.0
6	0.0	0.0	0.0	0.12865137353157396	0.0
7	0.0	0.0	0.0	0.1541566021034312	0.0
8	0.0	0.0	0.0	0.34038227836706525	0.0
9	0.0	0.0	0.0	0.4095839647127661	0.0
10	0.0	0.0	0.0	0.5339219540005701	0.0
11	0.0	0.0	0.0	0.6702624037927776	0.0
12	0.0	0.0	0.0	0.7840982401392286	0.0
13	0.0	0.0	0.0	0.8360463895098496	0.0
14	0.0	0.0	0.0	0.8549877724933611	0.0
15	0.0	0.0	0.0	0.887056846653564	0.0
16	0.0	0.0	0.0	0.9508199180832071	0.0
17	0.0	0.0	0.0	1.0320240649333112	0.0
18	0.0	0.0	0.0	1.1458599012797623	0.0
19	0.0	0.0	0.0	1.1972454353142394	0.0
20	0.0	0.0	0.0	1.257070199390875	0.0
21	0.0	0.0	0.0	1.3446506533839437	0.0
22	0.0	0.0	0.0	1.4275426462424796	0.0
23	0.0	0.0	0.0	1.5156857155716923	0.0
24	0.0	0.0	0.0	1.5948269395226022	0.0
25	0.0	0.0	0.0	1.6477127811201295	0.0
26	0.0	0.0	0.0	1.7062247760790963	0.0
27	0.0	0.0	0.0	1.7570476947774294	0.0
28	0.0	0.0	0.0	1.816872458854065	0.0
29	0.0	0.0	0.0	1.8766972229307008	0.0
30	0.0	0.0	0.0	1.9657779844868197	0.0
31	0.0	0.0	0.0	2.043981516210823	0.0
32	0.0	0.0	0.0	2.1216224325986826	0.0
33	0.0	0.0	0.0	2.188573657599808	0.0
34	0.0	0.0	0.0	2.262463805080041	0.0
35	0.0	0.0	0.0	2.361109027350607	0.0
36	0.0	0.0	0.0	2.4488770197890566	0.0
37	0.0	0.0	0.0	2.534582089328312	0.0
38	0.0	0.0	0.0	2.6088473136993082	0.0
39	0.0	0.0	0.0	2.685925614751024	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTA	30	2.163304E-6	45.000004	22
GACAACG	30	2.163304E-6	45.000004	12
CGTTATT	195	0.0	45.000004	1
CGTTAAG	65	0.0	45.000004	2
CGTAAAG	35	1.2102828E-7	45.000004	2
CGTCAAG	35	1.2102828E-7	45.000004	2
TTGGGAC	60	0.0	45.000004	5
ACGCGAT	20	7.02988E-4	45.0	26
AACGTAC	20	7.02988E-4	45.0	21
TAGCGAA	25	3.8879825E-5	45.0	1
TTCACGA	20	7.02988E-4	45.0	27
TGTATCG	20	7.02988E-4	45.0	13
TCTAGAT	20	7.02988E-4	45.0	21
CTGGTCG	25	3.8879825E-5	45.0	11
CGTTAGT	20	7.02988E-4	45.0	30
AAGCACG	20	7.02988E-4	45.0	1
TCGTCCG	20	7.02988E-4	45.0	17
GGTATCG	20	7.02988E-4	45.0	8
GTCGTAA	20	7.02988E-4	45.0	1
TCCGTTC	20	7.02988E-4	45.0	15
>>END_MODULE