##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513960
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.982387734454043	31.0	31.0	33.0	30.0	33.0
2	31.140197680753367	31.0	31.0	33.0	30.0	34.0
3	31.10161880301969	31.0	31.0	34.0	28.0	34.0
4	35.099178924429914	35.0	35.0	37.0	32.0	37.0
5	32.776054556774845	35.0	35.0	37.0	28.0	37.0
6	33.9603743481983	35.0	35.0	37.0	28.0	37.0
7	15.366417620048253	0.0	0.0	35.0	0.0	37.0
8	24.920236983422836	17.0	17.0	35.0	17.0	37.0
9	32.56454587905674	32.0	32.0	37.0	27.0	39.0
10	35.43066581056892	37.0	34.0	37.0	32.0	39.0
11	36.42020390691883	37.0	35.0	39.0	32.0	39.0
12	36.27674138065219	37.0	35.0	39.0	32.0	39.0
13	35.93758463693673	37.0	35.0	39.0	31.0	39.0
14	36.98917230912912	39.0	35.0	40.0	31.0	41.0
15	37.35676706358471	39.0	35.0	41.0	32.0	41.0
16	37.40767958596	39.0	35.0	41.0	32.0	41.0
17	37.36677173320881	39.0	35.0	41.0	32.0	41.0
18	37.28203167561678	39.0	35.0	40.0	32.0	41.0
19	37.23365047863647	39.0	36.0	40.0	32.0	41.0
20	37.34851350299634	39.0	35.0	40.0	32.0	41.0
21	37.18118530624951	39.0	35.0	40.0	32.0	41.0
22	37.24500155654137	39.0	35.0	41.0	32.0	41.0
23	37.241228889407736	39.0	35.0	41.0	32.0	41.0
24	37.06845474355981	39.0	35.0	41.0	32.0	41.0
25	36.793419721379095	38.0	35.0	40.0	31.0	41.0
26	36.646978364075025	38.0	35.0	40.0	31.0	41.0
27	36.55714257918904	38.0	35.0	40.0	31.0	41.0
28	36.491902093548134	38.0	35.0	40.0	31.0	41.0
29	36.562446493890576	38.0	35.0	40.0	31.0	41.0
30	36.31558097906452	38.0	35.0	40.0	30.0	41.0
31	36.21473850105067	38.0	35.0	40.0	30.0	41.0
32	36.124068020857656	38.0	35.0	40.0	30.0	41.0
33	35.929399175033076	38.0	35.0	40.0	30.0	41.0
34	35.81227916569383	38.0	35.0	40.0	29.0	41.0
35	35.658202972994005	38.0	34.0	40.0	28.0	41.0
36	35.42923184683633	38.0	34.0	40.0	27.0	41.0
37	35.28205113238384	38.0	34.0	40.0	27.0	41.0
38	35.235296521130046	38.0	34.0	40.0	27.0	41.0
39	35.16017783485096	38.0	34.0	40.0	26.0	41.0
40	34.96052221962799	38.0	34.0	40.0	25.0	41.0
41	34.841843723246946	38.0	34.0	40.0	24.0	41.0
42	34.793499494124056	38.0	34.0	40.0	24.0	41.0
43	34.71509650556464	38.0	33.0	40.0	24.0	41.0
44	34.52146081407113	37.0	33.0	40.0	24.0	41.0
45	34.44178535294576	37.0	33.0	40.0	23.0	41.0
46	34.39900770487976	37.0	33.0	40.0	23.0	41.0
47	34.32910537785042	37.0	33.0	40.0	23.0	41.0
48	34.28296754611254	37.0	33.0	40.0	23.0	41.0
49	34.17285975562301	36.0	33.0	40.0	24.0	41.0
50	34.070552183049266	36.0	33.0	40.0	24.0	41.0
51	33.88567203673438	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	4.0
14	4.0
15	28.0
16	71.0
17	175.0
18	326.0
19	559.0
20	1020.0
21	1675.0
22	2488.0
23	3618.0
24	5150.0
25	6792.0
26	8404.0
27	9264.0
28	10316.0
29	12160.0
30	14945.0
31	19198.0
32	25576.0
33	37949.0
34	48714.0
35	52005.0
36	60806.0
37	92847.0
38	83056.0
39	16807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.295898513503	24.39878589773523	29.0300801618803	14.27523542688147
2	31.72990115962332	25.753366020702	30.217915791112148	12.298817028562533
3	30.530586037823955	23.773834539652892	32.335006615300806	13.36057280722235
4	28.45240874776247	26.82290450618725	29.697641839831896	15.027044906218382
5	22.1439411627364	36.96844112382286	27.135380185228424	13.752237528212314
6	22.78290139310452	40.74208109580512	26.6174410459958	9.85757646509456
7	37.88660596155343	57.0484084364542	4.10187563234493	0.9631099696474434
8	86.31060782940307	4.3207642618102575	7.359327574130282	2.0093003346563933
9	80.15507043349676	6.842555840921473	9.227566347575687	3.7748073780060705
10	49.70834306171687	25.083080395361506	13.932990894233013	11.275585648688615
11	45.45353724025216	20.778854385555295	20.3973071834384	13.370301190754144
12	40.39808545412094	21.61452253093626	24.403650089501127	13.58374192544167
13	21.74332632889719	38.42439100319091	24.233208810024127	15.599073857887774
14	15.529224064129505	41.12168262121566	27.053661763561365	16.29543155109347
15	16.39154798038758	23.302007938360962	45.28581990816406	15.020624173087398
16	17.151918437232467	18.520118297143746	44.51941785352946	19.80854541209433
17	17.193361351077904	20.187563234492956	28.792902171375207	33.82617324305394
18	21.427348431784573	24.52817339870807	32.68269904272706	21.361779126780295
19	29.8941551871741	22.98077671414118	25.775157599813213	21.34991049887151
20	31.903260954159858	22.06864347420033	25.5031519962643	20.524943575375517
21	22.933496770176667	27.71305159934625	27.10230368122033	22.25114794925675
22	23.43937271382987	26.173826756946067	25.029379718266014	25.357420810958054
23	21.500895011284925	28.83765273562145	25.76601291929333	23.895439333800294
24	20.97089267647288	24.204607362440658	36.77581912989338	18.04868083119309
25	18.9139232625107	25.115378628687058	34.48770332321581	21.482994785586428
26	18.04945910187563	32.95353724025216	27.955677484629156	21.041326173243053
27	20.89248190520663	32.40952603315433	27.73017355436221	18.96781850727683
28	15.972060082496691	29.133979297999847	36.83380029574286	18.060160323760606
29	17.449996108646587	24.90485640905907	35.69499571951125	21.950151762783097
30	19.368627908786674	29.722351934002646	30.212467896334346	20.696552260876334
31	28.024554440034244	27.425480582146474	24.33224375437777	20.21772122344151
32	29.42991672503697	26.562962098217763	25.957662074869642	18.04945910187563
33	26.831465483695226	28.90750252937972	24.90582924741225	19.3552027395128
34	20.342049964977818	28.71410226476769	27.86870573585493	23.075142034399565
35	22.053661763561365	25.375515604327187	29.420188341505177	23.150634290606273
36	29.221145614444705	26.33064829947856	25.231730095727293	19.216475990349444
37	21.582224297610708	32.95256440189898	27.681142501361972	17.784068799128335
38	20.595571639816328	31.927387345318703	24.996497781928557	22.480543232936416
39	23.107440267725117	30.583897579578178	26.22791656938283	20.080745583313877
40	24.232819674682855	27.32352712273329	26.045217526655772	22.398435675928088
41	18.327496303214257	25.999883259397617	26.62425091446805	29.04836952292007
42	22.240252159701143	26.778348509611643	24.441007082263212	26.540392248424
43	23.389174254805823	27.566931278698732	25.340298855942095	23.70359561055335
44	21.257296287648845	30.494591018756324	26.74896879134563	21.4991439022492
45	18.847770254494513	35.5004280488754	22.960347108724413	22.691454587905675
46	22.01280255272784	33.17787376449529	25.060705113238384	19.748618569538483
47	23.577126624640048	27.57782706825434	26.808895633901468	22.03615067320414
48	23.45999688691727	24.48264456377928	29.646665110125305	22.410693439178146
49	20.58234103821309	25.8488987469842	31.305743637637168	22.263016577165537
50	19.516693906140556	32.75177056580279	26.836329675461123	20.895205852595534
51	19.75173165226866	32.51478714296832	24.168028640361115	23.565452564401898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	460.0
2	706.0
3	2541.5
4	4377.0
5	3335.0
6	2293.0
7	2372.0
8	2451.0
9	2463.0
10	2475.0
11	2423.0
12	2371.0
13	2239.0
14	2107.0
15	2159.5
16	2212.0
17	2042.5
18	1873.0
19	1828.5
20	1784.0
21	1889.0
22	1994.0
23	2307.0
24	2620.0
25	3245.5
26	4815.5
27	5760.0
28	6971.0
29	8182.0
30	9574.0
31	10966.0
32	12610.5
33	14255.0
34	14559.5
35	14864.0
36	16408.5
37	17953.0
38	22460.0
39	26967.0
40	33267.0
41	39567.0
42	43574.5
43	47582.0
44	46493.0
45	45404.0
46	42283.0
47	39162.0
48	36903.0
49	34644.0
50	32908.0
51	31172.0
52	29000.0
53	26828.0
54	25944.5
55	25061.0
56	24337.0
57	23613.0
58	21907.5
59	20202.0
60	18218.0
61	16234.0
62	13884.0
63	11534.0
64	9809.0
65	8084.0
66	6723.5
67	5363.0
68	4453.5
69	3544.0
70	2936.0
71	2328.0
72	1974.5
73	1621.0
74	1238.5
75	620.0
76	384.0
77	312.5
78	241.0
79	188.0
80	135.0
81	87.0
82	39.0
83	41.0
84	43.0
85	32.0
86	21.0
87	11.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.36236805208396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17727463971329	19.202164175840128
2	6.458656618449217	3.534482790320709
3	3.3271027427608812	2.7311222938356376
4	2.3816091379426285	2.6066586315437035
5	1.88255701427015	2.575560895174606
6	1.5761763723490998	2.5876750809128755
7	1.2896112499031638	2.4700772364770875
8	1.1513794748950974	2.520357516775585
9	1.0817676164772223	2.663975130198782
>10	10.491854754896458	54.28559176021598
>50	0.15802451453021568	2.71712353946884
>100	0.019047597733552784	0.9798322738238016
>500	0.002821866330896709	0.4746665802467943
>1k	0.002116399748172532	0.6507120951654917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1207	0.2348431784574675	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1140	0.22180714452486577	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1024	0.19923729473110746	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	729	0.14183983189353255	No Hit
AAGGAAAGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	620	0.12063195579422523	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	606	0.11790800840532337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007977274496069733	0.0
2	0.0	0.0	0.0	0.03988637248034867	0.0
3	0.0	0.0	0.0	0.04980932368277687	0.0
4	0.0	0.0	0.0	0.07354657950035022	0.0
5	0.0	0.0	0.0	0.12744182426647988	0.0
6	0.0	0.0	0.0	0.18230990738578878	0.0
7	0.0	0.0	0.0	0.21733208810024127	0.0
8	0.0	0.0	0.0	0.4266869017044128	0.0
9	0.0	0.0	0.0	0.49945521052221964	0.0
10	0.0	0.0	0.0	0.6399330687213013	0.0
11	0.0	0.0	0.0	0.7724336524243132	0.0
12	0.0	0.0	0.0	0.8924819052066308	0.0
13	0.0	0.0	0.0	0.9438477702544945	0.0
14	0.0	0.0	0.0	0.964861078683166	0.0
15	0.0	0.0	0.0	1.0033854774690638	0.0
16	0.0	0.0	0.0	1.087049575842478	0.0
17	0.0	0.0	0.0	1.1868627908786675	0.0
18	0.0	0.0	0.0	1.3213090512880379	0.0
19	0.0	0.0	0.0	1.3859055179391393	0.0
20	0.0	0.0	0.0	1.4501128492489688	0.0
21	0.0	0.0	0.0	1.5285236205152153	0.0
22	0.0	0.0	0.0	1.6174410459957973	0.0
23	0.0	0.0	0.0	1.717254261031987	0.0
24	0.0	0.0	0.0	1.7939139232625108	0.0
25	0.0	0.0	0.0	1.8590940929255195	0.0
26	0.0	0.0	0.0	1.9353646198147716	0.0
27	0.0	0.0	0.0	2.004825278231769	0.0
28	0.0	0.0	0.0	2.0702000155654137	0.0
29	0.0	0.0	0.0	2.14063351233559	0.0
30	0.0	0.0	0.0	2.2332477235582537	0.0
31	0.0	0.0	0.0	2.3204140400031132	0.0
32	0.0	0.0	0.0	2.406996653436065	0.0
33	0.0	0.0	0.0	2.4881313720912135	0.0
34	0.0	0.0	0.0	2.5784107712662463	0.0
35	0.0	0.0	0.0	2.691065452564402	0.0
36	0.0	0.0	0.0	2.7936026149894935	0.0
37	0.0	0.0	0.0	2.8951669390614057	0.0
38	0.0	0.0	0.0	2.983889796871352	0.0
39	0.0	0.0	0.0	3.0759203050821076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAA	35	1.2101737E-7	45.000004	1
ATCGTAG	35	1.2101737E-7	45.000004	2
TCTACGG	35	1.2101737E-7	45.000004	2
CTGTCCG	25	3.8877857E-5	45.0	21
ATAGGCG	50	2.1827873E-11	45.0	5
ACGTTAG	20	7.0296426E-4	45.0	2
CGTTGAC	20	7.0296426E-4	45.0	34
TACCTTA	20	7.0296426E-4	45.0	11
TATTACG	20	7.0296426E-4	45.0	1
AGCGAAA	45	3.8380676E-10	45.0	1
GCGATAT	55	1.8189894E-12	45.0	9
TCCGTAG	20	7.0296426E-4	45.0	2
ACCCGTT	20	7.0296426E-4	45.0	12
GATCTCG	20	7.0296426E-4	45.0	13
TATACTC	25	3.8877857E-5	45.0	43
TATCGCG	40	6.8030204E-9	45.0	32
CGATAGT	45	3.8380676E-10	45.0	10
TACAACG	25	3.8877857E-5	45.0	8
CGTACTT	30	2.1631513E-6	44.999996	29
ACGTACT	30	2.1631513E-6	44.999996	28
>>END_MODULE