FastQCFastQC Report
Sat 18 Jun 2016
SRR3549200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805026
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17710.21999289463942778No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.19452787860267867No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14790.1837207742358632No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12500.15527448802895807No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9250.11490312114142898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG600.045.0000042
TGCACGG302.1646883E-645.0000042
TCGTTAG253.8897608E-545.02
GTCGAAA207.0320256E-445.01
GCGAATA502.1827873E-1145.01
CGGTTTA253.8897608E-545.01
GACCGAC207.0320256E-445.09
TGCGCAA502.1827873E-1145.01
TCATGCG253.8897608E-545.034
CCGAATG207.0320256E-445.01
CCGGTGA207.0320256E-445.037
CGGTCTA700.045.031
CGCGTAG253.8897608E-545.02
TGCGTAA351.2113196E-745.01
CGTTTTA11350.042.819381
AATAGGC1750.042.428574
CGCGACC800.042.187510
TGATAAG2250.042.02
AGGCGAT6750.042.07
TAAGGCG1400.041.7857135