##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805026
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081891764986473	31.0	31.0	33.0	30.0	33.0
2	31.24333624007175	31.0	31.0	33.0	30.0	34.0
3	31.215201745036808	31.0	31.0	34.0	28.0	34.0
4	35.186123181114645	35.0	35.0	37.0	33.0	37.0
5	33.00290301182819	35.0	35.0	37.0	28.0	37.0
6	34.135954615130444	35.0	35.0	37.0	29.0	37.0
7	14.884118028486036	0.0	0.0	35.0	0.0	37.0
8	24.689560337181657	17.0	17.0	35.0	17.0	37.0
9	32.588922593804426	32.0	32.0	37.0	27.0	39.0
10	35.54606310852072	37.0	35.0	37.0	32.0	39.0
11	36.53430945087488	37.0	35.0	39.0	33.0	39.0
12	36.444113606268616	37.0	35.0	39.0	32.0	39.0
13	36.11911043866906	38.0	35.0	39.0	32.0	39.0
14	37.203219523344586	39.0	36.0	40.0	32.0	41.0
15	37.53266602569358	39.0	36.0	41.0	33.0	41.0
16	37.56639288668937	39.0	36.0	41.0	33.0	41.0
17	37.52488366835357	39.0	35.0	41.0	33.0	41.0
18	37.42622598524768	39.0	36.0	41.0	32.0	41.0
19	37.371046400985804	39.0	36.0	41.0	32.0	41.0
20	37.507781115144105	39.0	35.0	41.0	32.0	41.0
21	37.36874709636707	39.0	35.0	41.0	32.0	41.0
22	37.420083326501256	39.0	35.0	41.0	32.0	41.0
23	37.4070911995389	39.0	35.0	41.0	33.0	41.0
24	37.263146780352436	39.0	35.0	41.0	32.0	41.0
25	37.00079624757461	39.0	35.0	40.0	32.0	41.0
26	36.869997987642634	39.0	35.0	40.0	32.0	41.0
27	36.787249107482246	39.0	35.0	40.0	31.0	41.0
28	36.712580711678875	38.0	35.0	40.0	31.0	41.0
29	36.75687741762378	38.0	35.0	40.0	31.0	41.0
30	36.51600942081374	38.0	35.0	40.0	31.0	41.0
31	36.404624198473094	38.0	35.0	40.0	30.0	41.0
32	36.32941544745139	38.0	35.0	40.0	30.0	41.0
33	36.138503601125926	38.0	35.0	40.0	30.0	41.0
34	36.012676609202686	38.0	35.0	40.0	30.0	41.0
35	35.857769811161376	38.0	35.0	40.0	29.0	41.0
36	35.653470819576015	38.0	35.0	40.0	28.0	41.0
37	35.52875062420344	38.0	34.0	40.0	27.0	41.0
38	35.47285801949254	38.0	34.0	40.0	27.0	41.0
39	35.3796448810349	38.0	34.0	40.0	27.0	41.0
40	35.187083398548616	38.0	34.0	40.0	26.0	41.0
41	35.07967071870971	38.0	34.0	40.0	26.0	41.0
42	35.0577111795147	38.0	34.0	40.0	26.0	41.0
43	34.9572013823156	38.0	34.0	40.0	26.0	41.0
44	34.7556227997605	37.0	34.0	40.0	24.0	41.0
45	34.672259032627515	37.0	34.0	40.0	24.0	41.0
46	34.61239512761078	37.0	33.0	40.0	24.0	41.0
47	34.586231997475856	37.0	33.0	40.0	24.0	41.0
48	34.542028456223775	37.0	33.0	40.0	24.0	41.0
49	34.42246213165786	37.0	33.0	40.0	24.0	41.0
50	34.321615699368714	37.0	33.0	40.0	24.0	41.0
51	34.160158802324396	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	6.0
14	9.0
15	30.0
16	67.0
17	177.0
18	382.0
19	793.0
20	1353.0
21	2251.0
22	3219.0
23	5080.0
24	7517.0
25	9986.0
26	12147.0
27	13392.0
28	14955.0
29	17446.0
30	21670.0
31	28500.0
32	38601.0
33	58614.0
34	74861.0
35	81996.0
36	97740.0
37	150219.0
38	136431.0
39	27575.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.36830363242926	24.165554901332378	29.498301918198916	14.967839548039441
2	30.81627177258871	25.008633261534406	30.957882105671118	13.217212860205757
3	29.641899764728098	23.420734237155074	33.31606681026451	13.621299187852317
4	28.017728619945192	27.806182657454542	29.721027643827654	14.45506107877261
5	21.869852650721842	35.885673257758135	28.27051548645634	13.973958605063688
6	22.518775791092462	40.58291285001975	26.482126043134013	10.416185315753777
7	36.71918670949758	58.40991471082921	3.8870794235217248	0.9838191561514784
8	86.33770834730804	4.245328722302137	7.333179301041208	2.0837836293486176
9	81.01117230996266	6.527491037556551	8.838596517379562	3.6227401351012265
10	50.29216447667528	24.12518353444485	14.156934061757012	11.425717927122852
11	44.777932638200504	20.997582686770365	20.962676981861456	13.261807693167674
12	40.747628026920864	21.69681476126237	24.29002293093639	13.26553428088037
13	22.748333594194474	37.016071530608954	24.647402692583842	15.588192182612737
14	16.70517473969785	39.1922745352324	27.43725047389774	16.66530025117201
15	16.51983911078648	23.150556627984685	44.626384737884244	15.703219523344588
16	17.648001431009682	18.897526291076314	43.99534921853456	19.45912305937945
17	18.13991100908542	20.6293958207561	28.958567797810257	32.27212537234822
18	22.645852432095364	24.498711842847314	31.6366179477433	21.218817777314026
19	30.181261226345484	24.28704166076624	25.439178361941106	20.092518750947175
20	31.992010195943983	22.513807007475535	25.8139488662478	19.680233930332687
21	24.409894835694747	27.04111420003826	27.144464899270332	21.40452606499666
22	23.53948816559962	25.41830947075001	25.695070718212826	25.347131645437535
23	22.813673098757057	28.409268768959016	25.58376996519367	23.193288167090255
24	21.549862985791762	23.22521260182901	36.13672105000336	19.088203362375875
25	19.79141046376142	25.507250697493	33.01371632717453	21.687622511571057
26	18.61840487139546	33.02452343154134	27.1008638230318	21.256207874031396
27	19.94556697547657	31.970892865572043	28.739320220713367	19.34421993823802
28	17.098702402158438	28.65298760536927	36.17547756221538	18.07283243025691
29	18.56200917734334	24.967640796694766	35.151287039176374	21.319062986785518
30	20.582565035166567	28.73472409586771	29.863507514043025	20.819203354922696
31	28.4236782414481	26.25691592569681	25.577062107310823	19.742343725544266
32	29.310109238707817	27.015152305639816	26.179527120863177	17.495211334789186
33	27.535508169922462	28.084807198773703	25.24278718948208	19.136897441821755
34	21.49595168354811	27.997356607115798	28.21213227895745	22.294559430378648
35	23.00323219374281	25.424768889452015	29.626248096334777	21.945750820470394
36	28.324178349519148	27.15278761182869	25.576813668129972	18.946220370522195
37	22.080901734850798	32.21498436075357	27.67239815856879	18.031715745826844
38	21.793581822202015	31.79189243577226	24.684917008891638	21.729608733134086
39	23.35899710071476	29.60997532998934	26.93267049759884	20.098357071697063
40	24.420826159651988	27.40395962366433	25.239309040950232	22.935905175733453
41	17.902278932605903	26.506224643676106	27.869534648570355	27.721961775147637
42	23.077888167587133	26.498771468250716	24.71199687960389	25.711343484558263
43	23.84308084459384	27.2279404640347	25.123660602266263	23.805318089105196
44	21.54688171562161	30.439637974425672	26.820375987856295	21.193104322096428
45	19.600609172871437	34.485097375736935	23.000250923572654	22.914042527818978
46	21.104038875763017	33.03495787713689	25.417812592388323	20.443190654711774
47	23.50731529168002	27.45228104433894	27.127943693744054	21.912459970236988
48	23.436013246777122	23.964443384437274	29.853818385990017	22.745724982795586
49	20.42306708106322	26.037047250647806	30.706213215473788	22.833672452815186
50	19.511667946128448	32.24020093760947	26.87354197255741	21.374589143704675
51	20.01848387505497	31.988035169050438	24.74267911843841	23.25080183745618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	423.0
1	884.5
2	1346.0
3	4203.5
4	7061.0
5	5403.0
6	3745.0
7	3800.0
8	3855.0
9	3833.0
10	3811.0
11	3760.0
12	3709.0
13	3580.0
14	3451.0
15	3296.5
16	3142.0
17	3002.0
18	2862.0
19	2878.0
20	2894.0
21	3077.5
22	3261.0
23	3542.5
24	3824.0
25	5130.5
26	8068.5
27	9700.0
28	11835.5
29	13971.0
30	15787.0
31	17603.0
32	19710.5
33	21818.0
34	22826.0
35	23834.0
36	25456.0
37	27078.0
38	33512.0
39	39946.0
40	50891.0
41	61836.0
42	65272.5
43	68709.0
44	67161.0
45	65613.0
46	62771.0
47	59929.0
48	57625.0
49	55321.0
50	52965.5
51	50610.0
52	47347.5
53	44085.0
54	41582.0
55	39079.0
56	37731.0
57	36383.0
58	35160.0
59	33937.0
60	31131.0
61	28325.0
62	24443.5
63	20562.0
64	17019.5
65	13477.0
66	10956.5
67	8436.0
68	7160.0
69	5884.0
70	4874.0
71	3864.0
72	3199.5
73	2535.0
74	1948.0
75	1035.5
76	710.0
77	524.0
78	338.0
79	241.5
80	145.0
81	109.0
82	73.0
83	46.5
84	20.0
85	18.0
86	16.0
87	8.5
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	805026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.74446443058467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13131478521998	17.12719438248962
2	5.983231631166083	2.841372612923443
3	2.887036372780548	2.056533973898757
4	1.9812210976004156	1.8817213552438792
5	1.5418289280275783	1.8304951069798663
6	1.1905727263634889	1.6961707051897246
7	1.0821624837372323	1.798675802224831
8	0.9207564841564496	1.749029566982641
9	0.8116800627019345	1.7345617540056737
>10	10.919361740071665	57.15454365126159
>50	0.5061855287186099	7.4677468543069185
>100	0.039975677652487975	1.5066409490587287
>500	0.002595823224187531	0.4075400898043002
>1k	0.0020766585793500244	0.7477731956300318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1771	0.21999289463942778	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1566	0.19452787860267867	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1479	0.1837207742358632	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1250	0.15527448802895807	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	925	0.11490312114142898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007453175425389987	0.0
2	0.0	0.0	0.0	0.03453304613764027	0.0
3	0.0	0.0	0.0	0.043601076238531426	0.0
4	0.0	0.0	0.0	0.06136447766904423	0.0
5	0.0	0.0	0.0	0.09254359486525901	0.0
6	0.0	0.0	0.0	0.1321696442102491	0.0
7	0.0	0.0	0.0	0.15713778188530556	0.0
8	0.0	0.0	0.0	0.3165115163982281	0.0
9	0.0	0.0	0.0	0.384335412769277	0.0
10	0.0	0.0	0.0	0.5050768546605948	0.0
11	0.0	0.0	0.0	0.6402277690409999	0.0
12	0.0	0.0	0.0	0.7480503735283084	0.0
13	0.0	0.0	0.0	0.795502257069958	0.0
14	0.0	0.0	0.0	0.8135140976813171	0.0
15	0.0	0.0	0.0	0.8428299210211844	0.0
16	0.0	0.0	0.0	0.9203429454452403	0.0
17	0.0	0.0	0.0	1.0074208783318799	0.0
18	0.0	0.0	0.0	1.1341248605635097	0.0
19	0.0	0.0	0.0	1.1870424060837788	0.0
20	0.0	0.0	0.0	1.2501459580187473	0.0
21	0.0	0.0	0.0	1.3295222762991505	0.0
22	0.0	0.0	0.0	1.4117556451592868	0.0
23	0.0	0.0	0.0	1.5079016081468177	0.0
24	0.0	0.0	0.0	1.5806942881347932	0.0
25	0.0	0.0	0.0	1.6410650090804522	0.0
26	0.0	0.0	0.0	1.6983302402655318	0.0
27	0.0	0.0	0.0	1.7577072044878053	0.0
28	0.0	0.0	0.0	1.8198169996993885	0.0
29	0.0	0.0	0.0	1.88577760221409	0.0
30	0.0	0.0	0.0	1.9702469237018432	0.0
31	0.0	0.0	0.0	2.054343586418327	0.0
32	0.0	0.0	0.0	2.132850367565768	0.0
33	0.0	0.0	0.0	2.2031586557452805	0.0
34	0.0	0.0	0.0	2.2834045111586456	0.0
35	0.0	0.0	0.0	2.3845192577631034	0.0
36	0.0	0.0	0.0	2.4801683423889416	0.0
37	0.0	0.0	0.0	2.5683642515893896	0.0
38	0.0	0.0	0.0	2.661156285635495	0.0
39	0.0	0.0	0.0	2.7538241000911774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	60	0.0	45.000004	2
TGCACGG	30	2.1646883E-6	45.000004	2
TCGTTAG	25	3.8897608E-5	45.0	2
GTCGAAA	20	7.0320256E-4	45.0	1
GCGAATA	50	2.1827873E-11	45.0	1
CGGTTTA	25	3.8897608E-5	45.0	1
GACCGAC	20	7.0320256E-4	45.0	9
TGCGCAA	50	2.1827873E-11	45.0	1
TCATGCG	25	3.8897608E-5	45.0	34
CCGAATG	20	7.0320256E-4	45.0	1
CCGGTGA	20	7.0320256E-4	45.0	37
CGGTCTA	70	0.0	45.0	31
CGCGTAG	25	3.8897608E-5	45.0	2
TGCGTAA	35	1.2113196E-7	45.0	1
CGTTTTA	1135	0.0	42.81938	1
AATAGGC	175	0.0	42.42857	4
CGCGACC	80	0.0	42.1875	10
TGATAAG	225	0.0	42.0	2
AGGCGAT	675	0.0	42.0	7
TAAGGCG	140	0.0	41.785713	5
>>END_MODULE