##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178251
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551099292570587	31.0	31.0	33.0	30.0	34.0
2	31.74142641556008	31.0	31.0	34.0	30.0	34.0
3	31.806750032257884	31.0	31.0	34.0	30.0	34.0
4	35.704209233047784	37.0	35.0	37.0	35.0	37.0
5	27.219824853717512	35.0	26.0	37.0	0.0	37.0
6	31.408042591626415	35.0	28.0	37.0	17.0	37.0
7	14.826531127455105	0.0	0.0	32.0	0.0	37.0
8	24.98609825470825	17.0	17.0	35.0	17.0	37.0
9	33.09787322371263	32.0	32.0	37.0	28.0	39.0
10	36.047674346847984	37.0	35.0	39.0	32.0	39.0
11	37.0481512025178	39.0	37.0	39.0	34.0	39.0
12	37.006507677376284	39.0	35.0	39.0	33.0	39.0
13	36.51909386202602	39.0	35.0	39.0	33.0	39.0
14	37.82859002193536	40.0	36.0	41.0	33.0	41.0
15	38.11980858452407	40.0	36.0	41.0	33.0	41.0
16	38.184071898614874	40.0	36.0	41.0	34.0	41.0
17	38.22961442011545	40.0	36.0	41.0	34.0	41.0
18	38.21701421029896	40.0	36.0	41.0	34.0	41.0
19	38.15363167668063	40.0	36.0	41.0	34.0	41.0
20	38.16361759541321	40.0	36.0	41.0	34.0	41.0
21	38.02428597876029	40.0	36.0	41.0	34.0	41.0
22	37.941408463346626	40.0	35.0	41.0	34.0	41.0
23	37.857117211123644	40.0	35.0	41.0	34.0	41.0
24	37.74749650773348	40.0	35.0	41.0	33.0	41.0
25	37.560406393232014	39.0	35.0	41.0	33.0	41.0
26	37.2871007736282	39.0	35.0	40.0	33.0	41.0
27	37.08470639715906	39.0	35.0	40.0	33.0	41.0
28	37.09060257726464	39.0	35.0	40.0	33.0	41.0
29	37.099718935658146	39.0	35.0	40.0	33.0	41.0
30	36.955899265642266	39.0	35.0	40.0	32.0	41.0
31	36.79587772298613	38.0	35.0	40.0	32.0	41.0
32	36.588282814682664	38.0	35.0	40.0	31.0	41.0
33	36.38531060134305	38.0	35.0	40.0	31.0	41.0
34	36.232352132666854	38.0	35.0	40.0	31.0	41.0
35	36.130703334062645	38.0	35.0	40.0	30.0	41.0
36	35.92852775019495	38.0	35.0	40.0	30.0	41.0
37	35.78333361383667	38.0	35.0	40.0	30.0	41.0
38	35.5887821106193	38.0	35.0	40.0	29.0	41.0
39	35.53630554667295	38.0	34.0	40.0	29.0	41.0
40	35.273501971938444	38.0	34.0	40.0	28.0	41.0
41	35.109940477192275	38.0	34.0	40.0	27.0	40.0
42	35.13475380222271	38.0	34.0	40.0	27.0	41.0
43	35.00834216918839	37.0	34.0	40.0	27.0	41.0
44	34.81920999040678	37.0	34.0	40.0	27.0	40.0
45	34.63115494443229	37.0	34.0	40.0	26.0	40.0
46	34.57100380923529	37.0	34.0	40.0	26.0	40.0
47	34.430623110108776	36.0	33.0	39.0	26.0	40.0
48	34.355706279347665	36.0	33.0	39.0	26.0	40.0
49	34.190304682722676	36.0	33.0	39.0	26.0	40.0
50	34.315459660815364	36.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	12.0
17	25.0
18	59.0
19	109.0
20	211.0
21	349.0
22	538.0
23	835.0
24	1323.0
25	1868.0
26	2439.0
27	2666.0
28	2978.0
29	3317.0
30	4160.0
31	5591.0
32	7797.0
33	15023.0
34	15247.0
35	18284.0
36	24529.0
37	45691.0
38	20236.0
39	4961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.265176632950165	24.41669331448351	28.579923815294162	14.738206237272161
2	32.01440665129508	25.306449893689237	29.668837762480997	13.010305692534684
3	30.359997980375987	23.519643648562983	32.668540428945704	13.451817942115332
4	28.112605258876528	26.675867176060724	29.829846676876986	15.38168088818576
5	18.142394713073138	48.13605533769796	22.249524546846864	11.472025402382034
6	22.862704837560518	40.65110434163062	26.642206775838567	9.843984044970295
7	38.72404642891204	56.506274859608084	3.574734503593248	1.1949442078866317
8	86.97791316738756	3.9264856859148054	7.277939534701068	1.8176616119965665
9	82.3928056504592	5.918620372396228	8.737678891002014	2.950895086142574
10	51.16661337103297	24.675878396194133	13.507357602481893	10.650150630290995
11	46.784590268778295	20.091892892606495	20.023450078821437	13.100066759793775
12	41.380413013110726	22.06327033228425	23.742924303369968	12.813392351235056
13	22.489635401764925	37.85224206315813	23.71711799653298	15.941004538543963
14	16.094720366225154	40.62361501478253	27.12635553236728	16.15530908662504
15	15.981397018810553	22.99117536507509	45.72877571514326	15.298651900971102
16	16.86442151797185	17.970726671940128	45.43873526656232	19.726116543525702
17	17.630756629696325	18.359504294506063	29.669959775821734	34.33977929997587
18	21.646442376199854	23.62623491593315	32.47331010765718	22.254012600209816
19	30.64442836225323	23.134793072689636	25.111219572400717	21.10955899265642
20	33.06573315156717	21.798475183869936	24.402107141053907	20.733684523508984
21	24.520479548501832	26.20013351958755	26.068857958721132	23.210528973189493
22	23.498886401759318	25.960583671339855	24.495795255005582	26.04473467189525
23	21.889919271140133	29.052852438415496	25.664933156055227	23.392295134389148
24	21.09441181255645	22.37406802766885	37.09207802480772	19.439442134966985
25	19.369316301170823	24.44923170136493	34.59279330831244	21.588658689151814
26	19.115180279493522	33.81523806318057	26.919344071001007	20.150237586324902
27	20.71461029671643	31.591968628506994	28.471088521242514	19.22233255353406
28	17.699199443481383	28.43406208099814	36.650565775227065	17.216172700293406
29	18.119393439588	25.17124728613023	36.041312531205996	20.668046743075774
30	20.212509326735894	27.940376211073147	30.133351285546784	21.71376317664417
31	27.768708169940144	26.813313810301203	24.62819282921274	20.789785190545913
32	30.49744461461647	27.154966872556113	25.608832489018297	16.738756023809124
33	27.51681617494432	28.399279667435245	25.02875159185643	19.055152565764008
34	21.49553158187051	27.784977363380854	28.45257530112033	22.26691575362831
35	23.172941526274748	24.878401804197452	29.009093918126688	22.939562751401112
36	28.16758391257272	26.776287370056828	24.909257171067765	20.146871546302688
37	22.458780034894616	32.2864948864242	26.857633337260378	18.397091741420805
38	22.432973728057625	31.686217749129035	23.575183308929542	22.305625213883793
39	23.294118967074517	30.795339156582575	25.576294102136877	20.334247774206034
40	24.582751288912824	27.009105138260093	25.795647710251274	22.612495862575805
41	19.095545046030598	25.72608288312548	26.600692282231236	28.577679788612688
42	22.9580759715233	27.512328121581366	23.270556686919008	26.259039219976327
43	23.890469057677098	28.421719934250017	24.678683429545977	23.009127578526908
44	22.412216481253964	30.930541764141577	26.227061839765277	20.430179914839186
45	18.811114664153354	35.19363145227797	22.308991253906008	23.686262629662664
46	21.213906233345114	33.832068263291646	25.143196952611767	19.81082855075147
47	24.702806716371857	27.29914558684103	25.997610111584223	22.00043758520289
48	23.997060325047265	23.462981974855683	29.225081486218873	23.314876213878183
49	20.80156633062367	26.7695552900124	29.83826177693253	22.590616602431403
50	19.79848640400334	32.59673157513843	26.146276879232094	21.458505141626134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	154.5
2	259.0
3	963.5
4	1668.0
5	1293.0
6	918.0
7	910.5
8	903.0
9	908.5
10	914.0
11	880.0
12	846.0
13	819.5
14	793.0
15	744.5
16	696.0
17	678.0
18	660.0
19	669.5
20	679.0
21	644.5
22	610.0
23	726.0
24	842.0
25	1092.5
26	1343.0
27	1817.5
28	2292.0
29	2679.5
30	3067.0
31	3275.5
32	3484.0
33	3918.0
34	4352.0
35	4571.5
36	4791.0
37	5599.5
38	6408.0
39	8647.5
40	10887.0
41	13159.0
42	15431.0
43	16191.5
44	16952.0
45	15865.0
46	14778.0
47	13898.0
48	13018.0
49	12863.5
50	12709.0
51	11884.0
52	11059.0
53	10054.5
54	9050.0
55	8572.0
56	8094.0
57	8133.0
58	8172.0
59	7541.5
60	6911.0
61	6072.5
62	5234.0
63	4441.5
64	3649.0
65	3005.0
66	2361.0
67	1975.5
68	1590.0
69	1340.5
70	1091.0
71	904.0
72	717.0
73	603.0
74	489.0
75	361.0
76	233.0
77	188.0
78	143.0
79	101.0
80	59.0
81	40.0
82	21.0
83	16.0
84	11.0
85	9.5
86	8.0
87	7.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	178251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.6480356351437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.299934434046605	22.32526044734672
2	11.381653454133636	8.56993789656159
3	7.402992191691006	8.361243415184207
4	5.331704118733981	8.029127466325575
5	3.94438815044406	7.424923282337827
6	2.8804315431841214	6.506555362943265
7	2.2962985039041546	6.051578953273754
8	1.7553793884484712	5.286926861560384
9	1.3813554270727784	4.6804786508911596
>10	4.302020623472611	21.33283964746341
>50	0.008940811825713775	0.256380048358775
>100	0.014901353042856292	1.1747479677533366
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.26367313507357604	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	435	0.24403790161065014	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	214	0.12005542745903248	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.10098120066647591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006732080044431728	0.0
2	0.0	0.0	0.0	0.01739120678144863	0.0
3	0.0	0.0	0.0	0.02524530016661898	0.0
4	0.0	0.0	0.0	0.03253838688142002	0.0
5	0.0	0.0	0.0	0.05946670705914693	0.0
6	0.0	0.0	0.0	0.09200509394056695	0.0
7	0.0	0.0	0.0	0.12117744079977111	0.0
8	0.0	0.0	0.0	0.24515991495138878	0.0
9	0.0	0.0	0.0	0.2911624619216722	0.0
10	0.0	0.0	0.0	0.39831473596221056	0.0
11	0.0	0.0	0.0	0.5060280166731183	0.0
12	0.0	0.0	0.0	0.6070092173395941	0.0
13	0.0	0.0	0.0	0.6468406909358152	0.0
14	0.0	0.0	0.0	0.6631098843765252	0.0
15	0.0	0.0	0.0	0.684428137850559	0.0
16	0.0	0.0	0.0	0.7551149783170922	0.0
17	0.0	0.0	0.0	0.8302898721465798	0.0
18	0.0	0.0	0.0	0.9279050327908399	0.0
19	0.0	0.0	0.0	0.967736506387061	0.0
20	0.0	0.0	0.0	1.0187881133906682	0.0
21	0.0	0.0	0.0	1.084986900494247	0.0
22	0.0	0.0	0.0	1.159039780982996	0.0
23	0.0	0.0	0.0	1.2392637348458073	0.0
24	0.0	0.0	0.0	1.3032184952679087	0.0
25	0.0	0.0	0.0	1.3492210422381923	0.0
26	0.0	0.0	0.0	1.3969066092195837	0.0
27	0.0	0.0	0.0	1.4384211028269127	0.0
28	0.0	0.0	0.0	1.4877896898194118	0.0
29	0.0	0.0	0.0	1.5450123701970817	0.0
30	0.0	0.0	0.0	1.605601090596967	0.0
31	0.0	0.0	0.0	1.6717998777005458	0.0
32	0.0	0.0	0.0	1.7379986648041246	0.0
33	0.0	0.0	0.0	1.7907332918188397	0.0
34	0.0	0.0	0.0	1.856371072252049	0.0
35	0.0	0.0	0.0	1.9539862328963091	0.0
36	0.0	0.0	0.0	2.038137233451706	0.0
37	0.0	0.0	0.0	2.1172391739737786	0.0
38	0.0	0.0	0.0	2.190170041121789	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	35	1.4368925E-7	44.0	21
TCACGAC	20	7.8385725E-4	44.0	25
TTGATTC	30	2.5156514E-6	44.0	43
ATGGGAC	40	8.245479E-9	44.0	5
TCGTTGA	55	1.8189894E-12	44.0	24
CTCACGA	20	7.8385725E-4	44.0	24
ACACGTG	35	1.4368925E-7	44.0	42
ACACGAC	35	1.4368925E-7	44.0	26
CATATCA	35	1.4368925E-7	44.0	29
TTAATCG	55	1.8189894E-12	44.0	20
TTTACGG	20	7.8385725E-4	44.0	2
CACGGGA	20	7.8385725E-4	44.0	4
ACTTGCG	20	7.8385725E-4	44.0	13
TAGACCG	20	7.8385725E-4	44.0	22
GCGAAAA	20	7.8385725E-4	44.0	1
GCGCGAC	20	7.8385725E-4	44.0	9
AGCCATT	20	7.8385725E-4	44.0	12
TTAGACC	20	7.8385725E-4	44.0	21
CGTTGAT	55	1.8189894E-12	44.0	25
TAGCGAA	20	7.8385725E-4	44.0	1
>>END_MODULE