##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	940217
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543742561557597	31.0	31.0	33.0	30.0	34.0
2	31.732361784566756	31.0	31.0	34.0	30.0	34.0
3	31.802787016188816	31.0	31.0	34.0	30.0	34.0
4	35.697276267074514	37.0	35.0	37.0	35.0	37.0
5	27.398066616536397	35.0	28.0	37.0	0.0	37.0
6	31.498027582994137	36.0	30.0	37.0	17.0	37.0
7	14.666607815004408	0.0	0.0	32.0	0.0	37.0
8	24.92598942584531	17.0	17.0	35.0	17.0	37.0
9	33.19646315690952	32.0	32.0	37.0	28.0	39.0
10	36.13672694707711	37.0	35.0	39.0	32.0	39.0
11	37.07873926976432	39.0	37.0	39.0	34.0	39.0
12	36.88402039103739	39.0	35.0	39.0	33.0	39.0
13	36.24884680876861	38.0	35.0	39.0	32.0	39.0
14	37.557308578764264	40.0	35.0	41.0	33.0	41.0
15	37.90254164730057	40.0	35.0	41.0	33.0	41.0
16	38.015078434021085	40.0	35.0	41.0	33.0	41.0
17	38.05215179049092	40.0	35.0	41.0	34.0	41.0
18	38.032739250619805	40.0	36.0	41.0	34.0	41.0
19	37.94107317778768	40.0	36.0	41.0	34.0	41.0
20	37.95587720707028	40.0	36.0	41.0	34.0	41.0
21	37.78999847907451	40.0	35.0	41.0	34.0	41.0
22	37.71890531653863	39.0	35.0	41.0	33.0	41.0
23	37.62316465241535	39.0	35.0	41.0	33.0	41.0
24	37.48845532467505	39.0	35.0	41.0	33.0	41.0
25	37.2946766544319	39.0	35.0	41.0	33.0	41.0
26	37.05432150237658	39.0	35.0	40.0	33.0	41.0
27	36.87686459615174	38.0	35.0	40.0	32.0	41.0
28	36.91757328361432	38.0	35.0	40.0	32.0	41.0
29	36.941729409274664	38.0	35.0	40.0	32.0	41.0
30	36.751474393677206	38.0	35.0	40.0	32.0	41.0
31	36.54160156644689	38.0	35.0	40.0	32.0	41.0
32	36.29459582202832	38.0	35.0	40.0	31.0	41.0
33	36.08464322597868	38.0	35.0	40.0	31.0	41.0
34	35.922640199017884	38.0	35.0	40.0	30.0	41.0
35	35.79478460823405	38.0	35.0	40.0	30.0	41.0
36	35.57640523411085	38.0	35.0	40.0	29.0	41.0
37	35.39743272031882	38.0	34.0	40.0	29.0	41.0
38	35.2158086909724	38.0	34.0	40.0	29.0	41.0
39	35.20052073085256	38.0	34.0	40.0	28.0	41.0
40	34.9703993865246	37.0	34.0	40.0	27.0	41.0
41	34.82223252717192	37.0	34.0	40.0	27.0	40.0
42	34.85192248172496	37.0	34.0	40.0	27.0	40.0
43	34.69279538659693	37.0	34.0	40.0	27.0	41.0
44	34.49515058757712	36.0	34.0	40.0	26.0	40.0
45	34.32608961548238	36.0	33.0	39.0	26.0	40.0
46	34.30520082066161	36.0	33.0	39.0	26.0	40.0
47	34.167242243014115	36.0	33.0	39.0	26.0	40.0
48	34.170559562313805	36.0	33.0	39.0	26.0	40.0
49	34.017004585111735	36.0	33.0	39.0	25.0	40.0
50	34.081748149629284	36.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	5.0
15	13.0
16	46.0
17	123.0
18	272.0
19	559.0
20	1154.0
21	1902.0
22	3300.0
23	4833.0
24	7514.0
25	10909.0
26	14126.0
27	15687.0
28	16318.0
29	18736.0
30	23331.0
31	32091.0
32	45356.0
33	88788.0
34	84801.0
35	98533.0
36	120024.0
37	225552.0
38	103922.0
39	22317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.645496731073784	24.00137415086092	29.6148655044527	16.738263613612602
2	29.18273122055866	25.246512241323018	32.85145875898861	12.719297779129713
3	28.05001398613299	24.163358033305077	35.26600774076623	12.520620239795708
4	26.997171929458837	27.115655215764022	32.067597161080904	13.819575693696242
5	17.968511524467225	47.06168895052951	24.25854882436714	10.71125070063613
6	21.404526827317525	38.65501261942722	28.545644250210323	11.394816303044935
7	38.110776554774056	56.927602883164205	3.9087785053875863	1.0528420566741508
8	88.28600206122628	2.805309838048025	7.359683987845359	1.5490041128803245
9	84.59685370504893	4.431742884887212	8.571000098913336	2.400403311150511
10	59.21632984725866	19.659185060470083	12.562100025845096	8.562385066426154
11	52.75143929539669	16.819415092473335	19.051133940356323	11.378011671773644
12	46.82131890829457	20.045478862858253	21.25551867281702	11.877683556030151
13	20.12226964626251	42.623564560096234	22.17211558608279	15.082050207558467
14	13.956671704510768	43.2217243466136	27.15543326700113	15.666170681874503
15	12.803427293911938	20.303610762196385	52.17869917263781	14.71426277125387
16	14.411034899390247	15.349967082067225	50.26339664141363	19.975601377128896
17	15.195428289426802	16.35473513029439	29.430120918894254	39.01971566138455
18	21.45483436270563	21.515671382244737	34.20540151901103	22.824092736038594
19	30.576664748669725	23.009369113725874	24.556990567071217	21.856975570533184
20	33.61670763238699	20.73106527535665	24.780236902757554	20.871990189498806
21	22.08032826464529	26.91495686634043	27.4282426290952	23.576472239919084
22	23.20900387889179	24.163996183859684	25.616426846142964	27.010573091105567
23	20.628216677639312	29.723670174013023	24.652606791836355	24.995506356511317
24	21.20893368233078	20.822852596794146	40.02809989608782	17.940113824787257
25	16.968955039102678	23.834497780831445	37.350420168960994	21.846127011104883
26	15.567895496465178	34.22752407157071	28.449496233316353	21.755084198647758
27	18.17399600305036	32.05674860165259	30.95349265116457	18.81576274413247
28	14.491548227696372	27.716580321351348	40.25602600250793	17.53584544844435
29	16.156376666237687	22.488638261167367	38.62076520633003	22.734219866264915
30	18.451272419026672	28.23699209863255	32.06344918247596	21.24828629986482
31	30.143892314221077	25.53580715941107	24.47435007024974	19.845950456118107
32	31.83084330532207	25.817125195566554	25.89317146999044	16.458860029120935
33	27.819109843791377	27.728492465037323	24.984657797083013	19.46773989408828
34	19.371485518768537	27.14756274349432	29.253991365822994	24.226960371914146
35	20.996110472369676	24.563265714191513	30.59304394623794	23.847579867200867
36	30.26705537126004	24.84426467506969	26.133222436948067	18.755457516722203
37	21.457812398627123	30.923818650375388	29.31461566851057	18.303753282486916
38	21.50227022059801	30.956683403937603	23.959149855831154	23.581896519633233
39	22.115532903574387	29.797695638347317	27.20095467322969	20.885816784848604
40	24.3248101236204	25.563566708536435	24.68876865659736	25.422854511245806
41	16.508635772380206	24.19739272955073	27.73572483798953	31.558246660079536
42	22.94576677511681	24.944135236865534	23.594659530725355	28.51543845729231
43	23.500745040772504	27.458022988310145	24.499769734008215	24.54146223690914
44	21.05237407960077	30.8399018524447	25.91965471800659	22.188069349947938
45	17.292922803990994	37.40296123129022	21.382085199480546	23.922030765238237
46	21.370279414220335	33.836656856874534	24.87255601632389	19.920507712581244
47	22.662002495168668	26.319668757318787	27.625856584171526	23.392472163341015
48	24.513702687783777	21.84070273139073	30.501469341651983	23.144125239173512
49	20.794561255539946	22.828240714643535	31.616318360548686	24.760879669267837
50	19.43019536979229	32.14502609503976	26.55238099289845	21.872397542269496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	727.0
2	1187.0
3	5318.0
4	9449.0
5	7177.0
6	4905.0
7	5109.5
8	5314.0
9	5515.0
10	5716.0
11	5629.5
12	5543.0
13	5175.0
14	4807.0
15	4530.0
16	4253.0
17	3952.0
18	3651.0
19	3377.5
20	3104.0
21	3195.5
22	3287.0
23	3557.5
24	3828.0
25	4884.0
26	5940.0
27	7548.5
28	9157.0
29	10804.5
30	12452.0
31	13771.5
32	15091.0
33	16927.5
34	18764.0
35	19961.5
36	21159.0
37	26494.5
38	31830.0
39	47268.0
40	62706.0
41	77696.5
42	92687.0
43	94815.5
44	96944.0
45	90083.0
46	83222.0
47	75237.0
48	67252.0
49	67125.0
50	66998.0
51	64141.5
52	61285.0
53	55336.0
54	49387.0
55	45437.5
56	41488.0
57	39650.0
58	37812.0
59	35944.5
60	34077.0
61	30244.5
62	26412.0
63	22213.5
64	18015.0
65	14988.5
66	11962.0
67	9950.0
68	7938.0
69	6600.5
70	5263.0
71	4264.0
72	3265.0
73	2576.0
74	1887.0
75	1412.0
76	937.0
77	678.0
78	419.0
79	353.5
80	288.0
81	226.0
82	164.0
83	110.5
84	57.0
85	37.0
86	17.0
87	16.5
88	16.0
89	15.0
90	14.0
91	7.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	940217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.40616151661319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86557386855203	13.595816831418912
2	5.428676148338356	1.9984218001540273
3	2.0544251736571515	1.1344224471038888
4	1.370802566322407	1.0092485377247233
5	1.0336524694727314	0.9512787152580588
6	0.8378465660027893	0.9252923531992233
7	0.8135931756691149	1.0482589180125985
8	0.7111048417428365	1.0470968457891447
9	0.6787465706891354	1.124380710805638
>10	11.036895706432563	48.03111785691616
>50	2.016934852533379	24.48129554942445
>100	0.14876089404052467	3.713832405440045
>500	5.974333093996975E-4	0.08236344037173463
>1k	0.00238973323759879	0.8571735883814038
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2974	0.3163099582330462	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2390	0.25419663758472777	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1219	0.12965092101078793	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.12890641203041425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011167634705605195	0.0
2	0.0	0.0	0.0	0.03584278948370429	0.0
3	0.0	0.0	0.0	0.04679770733777415	0.0
4	0.0	0.0	0.0	0.06604858240172215	0.0
5	0.0	0.0	0.0	0.10923010326339558	0.0
6	0.0	0.0	0.0	0.1724070081693907	0.0
7	0.0	0.0	0.0	0.21920471550716483	0.0
8	0.0	0.0	0.0	0.4344741692609259	0.0
9	0.0	0.0	0.0	0.5164765155277984	0.0
10	0.0	0.0	0.0	0.6580395802245652	0.0
11	0.0	0.0	0.0	0.8020488887139884	0.0
12	0.0	0.0	0.0	0.9389321826769778	0.0
13	0.0	0.0	0.0	0.9964720910172864	0.0
14	0.0	0.0	0.0	1.012957647011275	0.0
15	0.0	0.0	0.0	1.040929912988172	0.0
16	0.0	0.0	0.0	1.1093183807567828	0.0
17	0.0	0.0	0.0	1.1961068561832002	0.0
18	0.0	0.0	0.0	1.3245878345105437	0.0
19	0.0	0.0	0.0	1.376171671007863	0.0
20	0.0	0.0	0.0	1.4289254501886266	0.0
21	0.0	0.0	0.0	1.506460742573257	0.0
22	0.0	0.0	0.0	1.5943128022573512	0.0
23	0.0	0.0	0.0	1.6902481022997882	0.0
24	0.0	0.0	0.0	1.7661880182979035	0.0
25	0.0	0.0	0.0	1.8243660771928183	0.0
26	0.0	0.0	0.0	1.879353383314703	0.0
27	0.0	0.0	0.0	1.9354042736942643	0.0
28	0.0	0.0	0.0	1.9930505404603405	0.0
29	0.0	0.0	0.0	2.0523985420386994	0.0
30	0.0	0.0	0.0	2.1382297916332083	0.0
31	0.0	0.0	0.0	2.217466818830121	0.0
32	0.0	0.0	0.0	2.285855286598732	0.0
33	0.0	0.0	0.0	2.3521165858519897	0.0
34	0.0	0.0	0.0	2.4285882939789434	0.0
35	0.0	0.0	0.0	2.529735156883996	0.0
36	0.0	0.0	0.0	2.6132265211116157	0.0
37	0.0	0.0	0.0	2.69788782802268	0.0
38	0.0	0.0	0.0	2.7801028911410874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.855751E-4	44.0	27
TCGTTAA	50	2.7284841E-11	44.0	1
ATCTACG	25	4.4422202E-5	44.0	1
CCCTATG	25	4.4422202E-5	44.0	33
CGTGATA	20	7.855751E-4	44.0	14
CTATACG	20	7.855751E-4	44.0	1
CCGGACC	20	7.855751E-4	44.0	17
CGACATA	20	7.855751E-4	44.0	44
CGTTCAC	30	2.5272238E-6	44.0	21
TCTAGAC	20	7.855751E-4	44.0	43
ATGTACG	55	1.8189894E-12	44.0	1
GACTTAT	20	7.855751E-4	44.0	8
CGTTACA	20	7.855751E-4	44.0	38
TACATAA	40	8.309144E-9	44.0	1
ACGATTG	25	4.4422202E-5	44.0	26
CCACTCG	20	7.855751E-4	44.0	20
ACAGGTT	45	4.802132E-10	44.0	5
CCGTAGG	20	7.855751E-4	44.0	3
CCGTAGA	40	8.309144E-9	44.0	19
CATAGTA	20	7.855751E-4	44.0	1
>>END_MODULE