##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1514607
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258795846051154	31.0	31.0	33.0	30.0	34.0
2	31.600895809936176	31.0	31.0	34.0	30.0	34.0
3	31.65543735107523	31.0	31.0	34.0	30.0	34.0
4	35.50443910532567	37.0	35.0	37.0	33.0	37.0
5	35.430331432510215	37.0	35.0	37.0	33.0	37.0
6	35.4796405932364	37.0	35.0	37.0	33.0	37.0
7	35.88141412260738	37.0	35.0	37.0	35.0	37.0
8	35.80762138297261	37.0	35.0	37.0	35.0	37.0
9	37.60888930263758	39.0	37.0	39.0	35.0	39.0
10	37.1199908623161	39.0	37.0	39.0	33.0	39.0
11	36.77865545319677	39.0	35.0	39.0	32.0	39.0
12	35.987672709818455	37.0	35.0	39.0	32.0	39.0
13	35.637957569191215	37.0	35.0	39.0	31.0	39.0
14	36.55515655216172	38.0	35.0	41.0	31.0	41.0
15	36.87214373101405	38.0	35.0	41.0	32.0	41.0
16	37.007852862161606	38.0	35.0	41.0	32.0	41.0
17	36.949421863229205	38.0	35.0	41.0	32.0	41.0
18	36.83879052453871	38.0	35.0	40.0	32.0	41.0
19	36.712784900637594	38.0	35.0	40.0	32.0	41.0
20	36.54852975062178	38.0	35.0	40.0	32.0	41.0
21	36.39864598539423	38.0	35.0	40.0	31.0	41.0
22	36.282716242563254	37.0	35.0	40.0	31.0	41.0
23	36.23796271904197	37.0	35.0	40.0	31.0	41.0
24	36.23782274873944	37.0	35.0	40.0	31.0	41.0
25	36.175823167329874	37.0	35.0	40.0	31.0	41.0
26	36.05380471633896	37.0	35.0	40.0	31.0	41.0
27	35.96951090282826	37.0	35.0	40.0	30.0	41.0
28	35.95692281892266	37.0	35.0	40.0	30.0	41.0
29	35.92154004306067	37.0	35.0	40.0	30.0	41.0
30	35.80290134668597	37.0	35.0	40.0	30.0	41.0
31	35.5671682489253	36.0	34.0	40.0	30.0	41.0
32	35.35519180883226	36.0	34.0	40.0	29.0	41.0
33	35.14083587359625	36.0	34.0	40.0	28.0	41.0
34	34.93083486343322	36.0	34.0	40.0	27.0	41.0
35	34.70386047337692	36.0	34.0	40.0	25.0	41.0
36	34.44429479066187	36.0	33.0	40.0	24.0	41.0
37	34.25217102522304	36.0	33.0	40.0	23.0	41.0
38	34.154039958880425	35.0	33.0	40.0	23.0	41.0
39	34.04638629030501	36.0	33.0	40.0	23.0	41.0
40	33.7472426840758	35.0	33.0	40.0	22.0	41.0
41	33.661103507378485	35.0	33.0	40.0	21.0	41.0
42	33.43601145379627	35.0	33.0	40.0	20.0	41.0
43	33.32272992267961	35.0	33.0	40.0	19.0	41.0
44	33.051551986753	35.0	32.0	40.0	18.0	41.0
45	32.948308042944475	35.0	32.0	40.0	18.0	41.0
46	32.76687285876798	35.0	32.0	39.0	17.0	41.0
47	32.67667322282282	35.0	32.0	39.0	16.0	41.0
48	32.66226684545892	35.0	32.0	39.0	17.0	41.0
49	32.64763268623478	35.0	32.0	39.0	16.0	41.0
50	32.4822617352224	35.0	32.0	39.0	15.0	41.0
51	32.24086116068393	35.0	31.0	39.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	2.0
13	2.0
14	23.0
15	44.0
16	141.0
17	399.0
18	780.0
19	1577.0
20	2666.0
21	4633.0
22	7093.0
23	10264.0
24	15478.0
25	22265.0
26	29394.0
27	33451.0
28	35698.0
29	39640.0
30	46611.0
31	55676.0
32	69686.0
33	88631.0
34	153296.0
35	214942.0
36	105209.0
37	129628.0
38	183741.0
39	263600.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.4704078351678	23.840375754238558	29.82826568212084	14.8609507284728
2	31.23569348352411	25.14038295082487	30.692384229044233	12.93153933660679
3	28.40109678616301	25.504635856033943	33.20953884406978	12.884728513733265
4	25.578120264860786	27.423483451482795	32.36258646632427	14.635809817332152
5	22.150762541042	32.67857602665246	31.156663081578255	14.013998350727283
6	19.953558910001075	40.48277870100957	28.60326144009634	10.960400948893014
7	86.35520633405234	4.165436974739982	7.872603256158198	1.606753435049488
8	87.8165755209107	3.2082909956180052	7.009343017693699	1.965790465777591
9	84.22950640001004	4.492980687399438	8.568031179045125	2.7094817335454016
10	50.162451381777586	25.386453383617003	13.683681641508324	10.767413593097087
11	41.78674732125231	21.334973362727098	23.23896561946432	13.639313696556268
12	39.159795247215946	20.5931967830599	25.718816828391788	14.528191141332373
13	22.35774692709066	37.18020582236844	26.16137387454303	14.300673375997865
14	15.958991342308599	40.107367785834874	27.647832077892154	16.285808793964375
15	13.707516207174534	23.7074039668376	46.96195118601723	15.623128639970632
16	16.456150011191024	17.990079274689737	45.12952865000624	20.424242064113002
17	17.301847938112	18.846473045483087	29.744613619242482	34.10706539716244
18	21.44490286919313	22.949979763727487	33.76704319998521	21.83807416709417
19	27.0763967154516	24.83574947164512	26.616079286574006	21.47177452632927
20	29.425983109810005	23.281022733950127	25.601822783071782	21.69117137316809
21	22.757124455386776	27.46692706424835	28.230623521481153	21.545324958883725
22	22.97143747519984	23.82624667653061	26.042399117394808	27.159916730874738
23	19.17778011061615	29.503627013476102	25.59396595948652	25.724626916421222
24	20.13089864235409	23.87008643166181	37.79303806201873	18.205976863965372
25	18.371102206711047	24.537256199132845	35.18807188927557	21.90356970488054
26	17.118632094001942	31.836905547115524	28.612570785688963	22.431891573193575
27	17.34608383560884	31.89962808834239	29.871181105065535	20.88310697098323
28	15.23022143698002	27.138128900764357	38.58413436620853	19.047515296047095
29	16.623124018309703	22.934134069101752	37.723845195486355	22.71889671710219
30	19.42741582469908	27.691671833023353	32.747636845729616	20.13327549654795
31	26.980398215510693	25.364533506051405	26.596602286929876	21.058465991508026
32	27.771560543428098	26.383213599303318	26.952470178732835	18.892755678535753
33	26.60604367997771	26.82808147592082	26.68586636665485	19.880008477446626
34	19.751592327250567	26.352842684603996	30.23556605772983	23.65999893041561
35	20.881060235427405	24.621766570470097	31.42465339193599	23.072519802166504
36	27.563651825192935	25.24965222001483	27.51789738196113	19.668798572831104
37	21.48326265493293	29.91165365008877	28.76970725739416	19.83537643758414
38	21.95645471069393	29.732729348273185	26.13503040722775	22.17578553380514
39	20.911959339947593	29.03400023900589	27.018031740246812	23.036008680799704
40	23.66323409306837	25.126848086665387	26.53440793552387	24.67550988474238
41	18.6675487436675	23.227213395950237	28.603459511279162	29.501778349103102
42	21.85761719046591	25.12348087655742	25.231363647467624	27.787538285509044
43	22.143698002188025	26.45577367594366	25.591985247658304	25.80854307421001
44	21.100457082266225	28.61673028052822	27.525820229273997	22.756992407931563
45	18.894340247998326	32.70855079898614	24.441257699191933	23.9558512538236
46	22.91835439820363	29.174102588988433	27.16731138836675	20.74023162444119
47	21.953417619224	27.049194939677424	27.774465587442815	23.222921853655766
48	22.55892122510988	23.33331352621505	30.482230704070428	23.62553454460464
49	20.50089561186499	23.42739733805535	31.589976805864488	24.481730244215168
50	19.157246731330304	28.828006208871344	29.203945313866896	22.810801745931453
51	19.135062758854275	30.395871668360176	25.905135787699386	24.563929785086163
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	739.0
1	1168.5
2	1598.0
3	7683.5
4	13769.0
5	10424.0
6	7079.0
7	7408.5
8	7738.0
9	8269.0
10	8800.0
11	8658.5
12	8517.0
13	8401.0
14	8285.0
15	7808.0
16	7331.0
17	7010.0
18	6689.0
19	6362.0
20	6035.0
21	5848.5
22	5662.0
23	5434.0
24	5206.0
25	5850.5
26	9001.5
27	11508.0
28	12792.5
29	14077.0
30	19094.0
31	24111.0
32	26434.0
33	28757.0
34	34083.5
35	39410.0
36	42385.5
37	45361.0
38	50900.5
39	56440.0
40	76854.0
41	97268.0
42	114693.0
43	132118.0
44	131471.0
45	130824.0
46	132007.5
47	133191.0
48	130036.0
49	126881.0
50	122389.5
51	117898.0
52	110346.0
53	102794.0
54	89437.0
55	76080.0
56	72528.5
57	68977.0
58	62048.0
59	55119.0
60	50462.5
61	45806.0
62	41085.0
63	36364.0
64	31206.0
65	26048.0
66	21950.5
67	17853.0
68	14854.5
69	11856.0
70	10114.5
71	8373.0
72	7374.5
73	6376.0
74	5078.5
75	2794.0
76	1807.0
77	1275.5
78	744.0
79	625.5
80	507.0
81	337.0
82	167.0
83	136.5
84	106.0
85	68.0
86	30.0
87	22.5
88	15.0
89	11.0
90	7.0
91	5.0
92	3.0
93	4.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1514607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.37781661599143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.09426370478938	18.81846011642601
2	6.133878971631132	2.7452563754369512
3	1.9120689764203047	1.2836378673438007
4	0.943914373091643	0.8449097096897321
5	0.5962440453886927	0.6671319953042517
6	0.42720331594856925	0.5735926477224317
7	0.3167343219137932	0.4961475800244082
8	0.2731908639092172	0.48907320429797885
9	0.22818915846179194	0.45957376276338513
>10	2.8589895904072513	15.133632858750252
>50	1.0573604412179138	17.173486720658417
>100	1.1508399678086125	39.56622459755261
>500	0.006231985385243403	0.8905403866959779
>1k	5.935224176422288E-4	0.3498409214850419
>5k	2.967612088211144E-4	0.5084912558487027
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7657	0.5055436822885409	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3734	0.24653259888538742	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1534	0.10128039814948696	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06093990058147097	0.0
2	0.0	0.0	0.0	0.26072770032094134	0.0
3	0.0	0.0	0.0	0.38524845058817236	0.0
4	0.0	0.0	0.0	0.5791601385705994	0.0
5	0.0	0.0	0.0	0.9356882676496279	0.0
6	0.0	0.0	0.0	1.295055417015767	0.0
7	0.0	0.0	0.0	1.5045487047135	0.0
8	0.0	0.0	0.0	2.0810018704522033	0.0
9	0.0	0.0	0.0	2.361074522962062	0.0
10	0.0	0.0	0.0	2.8389542633831746	0.0
11	0.0	0.0	0.0	3.238067696768865	0.0
12	0.0	0.0	0.0	3.585286480255274	0.0
13	0.0	0.0	0.0	3.720833193032912	0.0
14	0.0	0.0	0.0	3.7756989106745182	0.0
15	0.0	0.0	0.0	3.8544652177099405	0.0
16	0.0	0.0	0.0	4.051281949707086	0.0
17	0.0	0.0	0.0	4.288373155544639	0.0
18	0.0	0.0	0.0	4.670584514662879	0.0
19	0.0	0.0	0.0	4.819269949234356	0.0
20	0.0	0.0	0.0	4.995289207035224	0.0
21	0.0	0.0	0.0	5.175996149496206	0.0
22	0.0	0.0	0.0	5.361258729162087	0.0
23	0.0	0.0	0.0	5.565073976285597	0.0
24	0.0	0.0	0.0	5.7162022887785415	0.0
25	0.0	0.0	0.0	5.836695591661732	0.0
26	0.0	0.0	0.0	5.953623613254131	0.0
27	0.0	0.0	0.0	6.086397329472265	0.0
28	0.0	0.0	0.0	6.213426981388571	0.0
29	0.0	0.0	0.0	6.350162121263139	0.0
30	0.0	0.0	0.0	6.539518172040668	0.0
31	0.0	0.0	0.0	6.70543579951763	0.0
32	0.0	0.0	0.0	6.8497636680670295	0.0
33	0.0	0.0	0.0	6.998977292459364	0.0
34	0.0	0.0	0.0	7.1545291947019924	0.0
35	0.0	0.0	0.0	7.338207204905299	0.0
36	0.0	0.0	0.0	7.497522459621539	0.0
37	0.0	0.0	0.0	7.657894093979494	0.0
38	0.0	0.0	0.0	7.812719735218443	0.0
39	0.0	0.0	0.0	7.975336176315044	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	35	1.2122655E-7	45.0	1
CACGGTA	20	7.033997E-4	45.0	40
CAACGTA	20	7.033997E-4	45.0	20
GTTTACG	50	2.1827873E-11	45.0	1
CGACCGT	20	7.033997E-4	45.0	30
ATCCGTC	20	7.033997E-4	45.0	26
TGCGCAA	25	3.8913957E-5	45.0	15
CTTATCG	35	1.2122655E-7	45.0	1
ACGGATA	25	3.8913957E-5	45.0	25
ACTACGT	25	3.8913957E-5	45.0	45
ACGCTAG	20	7.033997E-4	45.0	1
CGTACAA	20	7.033997E-4	45.0	39
CGACTAT	20	7.033997E-4	45.0	27
ACGCGTA	20	7.033997E-4	45.0	23
TCGATAG	65	0.0	44.999996	1
ACGTCGG	30	2.1659616E-6	44.999996	2
CGGTCTA	125	0.0	43.199997	31
TGTTACG	120	0.0	43.124996	1
CGTTTTT	6885	0.0	43.104576	1
GTTACGG	215	0.0	42.906975	2
>>END_MODULE