##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1465457
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14418164436077	31.0	31.0	33.0	30.0	34.0
2	31.471349892900303	31.0	31.0	34.0	30.0	34.0
3	31.55701532013563	31.0	31.0	34.0	30.0	34.0
4	35.421343649114235	37.0	35.0	37.0	33.0	37.0
5	35.31829456613193	37.0	35.0	37.0	33.0	37.0
6	35.37980029437916	37.0	35.0	37.0	33.0	37.0
7	35.77038562032185	37.0	35.0	37.0	35.0	37.0
8	35.672650238116844	37.0	35.0	37.0	35.0	37.0
9	37.469390777075	39.0	37.0	39.0	35.0	39.0
10	36.95906669387092	39.0	37.0	39.0	32.0	39.0
11	36.65247973840243	39.0	35.0	39.0	32.0	39.0
12	35.731294742868606	37.0	35.0	39.0	31.0	39.0
13	35.27045283484947	37.0	35.0	39.0	30.0	39.0
14	36.08241729371793	38.0	35.0	40.0	30.0	41.0
15	36.440835179742564	38.0	35.0	40.0	31.0	41.0
16	36.599728958270354	38.0	35.0	40.0	32.0	41.0
17	36.54466217705467	38.0	35.0	40.0	31.0	41.0
18	36.498453383483785	38.0	35.0	40.0	31.0	41.0
19	36.37089795196993	37.0	35.0	40.0	31.0	41.0
20	36.2089047989808	37.0	35.0	40.0	31.0	41.0
21	36.020354060201015	37.0	34.0	40.0	31.0	41.0
22	35.91686211195552	37.0	34.0	40.0	30.0	41.0
23	35.893998936850416	36.0	34.0	40.0	30.0	41.0
24	35.868169451577224	36.0	34.0	40.0	30.0	41.0
25	35.776292992561366	36.0	34.0	40.0	30.0	41.0
26	35.668727912180294	36.0	34.0	40.0	30.0	41.0
27	35.56385687195189	36.0	34.0	40.0	30.0	41.0
28	35.559075428347604	36.0	34.0	40.0	30.0	41.0
29	35.5338034483441	36.0	34.0	40.0	30.0	41.0
30	35.39289927988334	36.0	34.0	40.0	29.0	41.0
31	35.147543053122675	36.0	34.0	40.0	29.0	41.0
32	34.9370053164303	35.0	34.0	40.0	27.0	41.0
33	34.72776615076389	36.0	34.0	40.0	26.0	41.0
34	34.52358684014611	35.0	34.0	40.0	25.0	41.0
35	34.35043471081035	35.0	33.0	40.0	24.0	41.0
36	34.104700444980644	35.0	33.0	40.0	23.0	41.0
37	33.91596205142832	35.0	33.0	40.0	23.0	41.0
38	33.834981169696555	35.0	33.0	40.0	23.0	41.0
39	33.80830621437545	35.0	33.0	40.0	23.0	41.0
40	33.58123302150797	35.0	33.0	40.0	23.0	41.0
41	33.53248713541237	35.0	33.0	40.0	22.0	41.0
42	33.400601996510304	35.0	32.0	39.0	22.0	41.0
43	33.326889154714195	35.0	32.0	39.0	22.0	41.0
44	33.163015359713725	35.0	32.0	39.0	22.0	41.0
45	33.11943441533938	35.0	32.0	39.0	22.0	41.0
46	33.0714418778579	35.0	32.0	39.0	22.0	41.0
47	33.015076525616244	35.0	32.0	39.0	22.0	41.0
48	32.96680694145239	35.0	32.0	39.0	21.0	41.0
49	32.97251369368054	35.0	32.0	39.0	21.0	40.0
50	32.80291472216517	35.0	32.0	38.0	20.0	40.0
51	32.5659449577845	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	4.0
14	21.0
15	60.0
16	175.0
17	488.0
18	1087.0
19	1960.0
20	3405.0
21	5335.0
22	7994.0
23	11367.0
24	16170.0
25	22359.0
26	29023.0
27	33441.0
28	35630.0
29	39406.0
30	45861.0
31	55790.0
32	68393.0
33	88955.0
34	156062.0
35	215461.0
36	102960.0
37	125384.0
38	172668.0
39	225940.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.31216337292735	23.149979835641716	28.367464893203966	14.170391898226969
2	31.171027194929636	25.33755681674727	30.642864307857547	12.848551680465548
3	29.46282286003615	24.591236726836748	32.712457615610695	13.233482797516405
4	26.47078692858269	26.80754194766547	30.706189263826918	16.015481859924925
5	21.487426789049422	33.24880907457537	31.185152481444355	14.07861165493085
6	19.58440268121139	41.616301263018975	28.49220413836776	10.307091917401875
7	87.11801165097303	4.426332536539796	6.838344625601433	1.6173111868857293
8	88.94085599236278	3.046216982142772	6.075033249013789	1.9378937764806474
9	85.2327976870014	3.938634842236927	8.131934270333419	2.6966332004282623
10	52.494682546127244	20.104104043994468	14.081614131291467	13.319599278586816
11	46.30241624285121	20.747521080454767	18.75346734841077	14.19659532828326
12	42.49807397965276	19.909966652040968	23.57216895480386	14.01979041350241
13	24.1482349874476	36.279263055824906	24.119029081030696	15.453472875696797
14	17.77930024558892	40.7388275466288	26.401593496090296	15.080278711691983
15	16.08515295911105	22.60994351932537	46.81290546225512	14.491998059308461
16	17.53528080318972	16.180413345461517	45.128038557255515	21.156267294093244
17	17.703692431780667	17.015306488010225	28.76065282024652	36.520348259962596
18	22.935916918749577	21.769318376451853	33.62753052460768	21.66723418019089
19	29.777741687405364	24.462130243330236	24.893940934466176	20.866187134798224
20	32.144307202463125	22.168511256215638	22.79848538715227	22.888696154168972
21	24.588575440971656	26.989396481780087	27.205847732140896	21.216180345107365
22	23.411263517114456	22.648293331022337	25.78990717571379	28.15053597614942
23	21.002663333008066	29.304783422509157	23.672274246190778	26.020278998292
24	21.814219045662888	24.752892783616307	34.65976824976782	18.773119920952986
25	19.72961335610666	22.665625808194985	34.22208908210886	23.382671753589495
26	18.86694730722225	30.06795832289859	27.083019153752037	23.982075216127118
27	19.72777092743083	30.491512204042838	28.062577066403176	21.71813980212316
28	16.37345892782934	27.531343464871366	36.31515629595409	19.780041311345197
29	17.381062699212602	25.034852609117834	35.87072155648375	21.713363135185816
30	20.373712773558008	26.877076570653387	32.339604642101406	20.4096060136872
31	29.90875883768681	25.017247179548768	23.536480428971988	21.537513553792433
32	29.71796511258945	25.727264600735474	25.24809666882072	19.30667361785436
33	28.45269427898601	25.989162425100155	24.575541964042618	20.982601331871216
34	20.45211834942956	26.359012922248827	27.87185157940492	25.317017148916687
35	20.262348195818777	25.561855448505145	30.255817809734438	23.919978545941643
36	30.82164812751244	23.738669916619866	25.581712735344674	19.857969220523017
37	22.542319563112397	29.84522916742013	27.172615777876796	20.439835491590678
38	22.58162470819683	31.517267309788004	22.07754987010878	23.823558111906387
39	21.451670025118442	29.210751321942574	24.882272219519237	24.455306433419747
40	23.417200231736583	24.942594699127984	25.400745296518423	26.239459772617007
41	19.38774047959101	23.01275301834172	26.835997234992227	30.76350926707505
42	22.90698396472909	25.830645320879427	23.435010375602968	27.827360338788516
43	23.025172352378814	25.558443543549895	24.928878841207897	26.487505262863394
44	22.343064313726025	27.405785362518316	26.95282086065985	23.29832946309581
45	19.275488806563413	33.39094903501092	23.398912421176465	23.9346497372492
46	23.990741454713444	29.37929942673173	26.37102282769129	20.258936290863534
47	23.365543990714162	27.19772739834741	25.87438594240568	23.56234266853275
48	23.243738983811877	24.83573383592968	27.876287055846742	24.044240124411704
49	21.5328733630533	23.291573891284425	30.819669222638396	24.355883523023873
50	21.44013778636971	28.817631632999124	27.103695297780828	22.638535282850334
51	20.442155586960244	31.543607216042503	23.629079529457364	24.385157667539886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	630.0
1	984.5
2	1339.0
3	6172.5
4	11006.0
5	8410.5
6	5815.0
7	6163.0
8	6511.0
9	6998.0
10	7485.0
11	7428.0
12	7371.0
13	7072.0
14	6773.0
15	6383.0
16	5993.0
17	5602.0
18	5211.0
19	5092.5
20	4974.0
21	4823.5
22	4673.0
23	4491.0
24	4309.0
25	4177.5
26	5093.0
27	6140.0
28	6967.5
29	7795.0
30	10093.5
31	12392.0
32	15510.0
33	18628.0
34	20126.5
35	21625.0
36	25000.5
37	28376.0
38	34817.5
39	41259.0
40	58809.0
41	76359.0
42	104428.5
43	132498.0
44	134987.0
45	137476.0
46	132661.5
47	127847.0
48	127390.0
49	126933.0
50	124798.5
51	122664.0
52	111871.0
53	101078.0
54	92464.0
55	83850.0
56	80614.0
57	77378.0
58	73387.0
59	69396.0
60	64434.5
61	59473.0
62	51806.5
63	44140.0
64	38522.0
65	32904.0
66	27709.5
67	22515.0
68	18633.5
69	14752.0
70	11907.0
71	9062.0
72	8139.5
73	7217.0
74	5425.5
75	2715.0
76	1796.0
77	1508.5
78	1221.0
79	881.0
80	541.0
81	353.5
82	166.0
83	129.0
84	92.0
85	66.5
86	41.0
87	32.5
88	24.0
89	23.0
90	22.0
91	19.5
92	17.0
93	12.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1465457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.016631040295113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5425935935359	15.506654164370042
2	6.875385722990626	2.6149334710765086
3	2.2527277074742966	1.2851787494186568
4	1.133038348627264	0.8618628892139978
5	0.7403878062768465	0.7039840869350145
6	0.528671429100024	0.6032129705244418
7	0.40264941271564053	0.5359924724143394
8	0.3564252009486602	0.5422405231922972
9	0.3048880840907537	0.52181497833627
>10	3.174037881455699	13.949602024130673
>50	1.2356150116589428	17.138364220480405
>100	1.4398667841336246	42.87682598307368
>500	0.010104328309709644	1.1889298783874969
>1k	0.002886950945631327	0.9151157245770432
>5k	7.217377364078317E-4	0.7552878638691366
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5990	0.40874621363847596	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5016	0.342282305110283	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2417	0.1649314855365937	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2270	0.15490048496817033	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1929	0.13163129317339234	No Hit
GTACGAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1632	0.11136457773923084	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1493	0.10187948196364685	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06605447993356339	0.0
2	0.0	0.0	0.0	0.2041001544228183	0.0
3	0.0	0.0	0.0	0.28748711152903156	0.0
4	0.0	0.0	0.0	0.42682930990127993	0.0
5	0.0	0.0	0.0	0.6815621338599495	0.0
6	0.0	0.0	0.0	0.9620207211811742	0.0
7	0.0	0.0	0.0	1.1337077785291552	0.0
8	0.0	0.0	0.0	1.6911448101172535	0.0
9	0.0	0.0	0.0	1.9495624914275889	0.0
10	0.0	0.0	0.0	2.3754364679414	0.0
11	0.0	0.0	0.0	2.681416104327865	0.0
12	0.0	0.0	0.0	2.9796165974163693	0.0
13	0.0	0.0	0.0	3.0972590802732527	0.0
14	0.0	0.0	0.0	3.139293749321884	0.0
15	0.0	0.0	0.0	3.2058941340482865	0.0
16	0.0	0.0	0.0	3.3635923810797586	0.0
17	0.0	0.0	0.0	3.5483811534558845	0.0
18	0.0	0.0	0.0	3.8785170769254913	0.0
19	0.0	0.0	0.0	3.988175702187099	0.0
20	0.0	0.0	0.0	4.1245836622978365	0.0
21	0.0	0.0	0.0	4.262834051084406	0.0
22	0.0	0.0	0.0	4.393168820374805	0.0
23	0.0	0.0	0.0	4.551822400793745	0.0
24	0.0	0.0	0.0	4.671716740921092	0.0
25	0.0	0.0	0.0	4.761313365045853	0.0
26	0.0	0.0	0.0	4.854526608423175	0.0
27	0.0	0.0	0.0	4.95319889972889	0.0
28	0.0	0.0	0.0	5.050915857647137	0.0
29	0.0	0.0	0.0	5.155729577872295	0.0
30	0.0	0.0	0.0	5.29350229996513	0.0
31	0.0	0.0	0.0	5.416945021245932	0.0
32	0.0	0.0	0.0	5.5218952176693	0.0
33	0.0	0.0	0.0	5.641857795895751	0.0
34	0.0	0.0	0.0	5.759090850158005	0.0
35	0.0	0.0	0.0	5.917812668676051	0.0
36	0.0	0.0	0.0	6.037297580208768	0.0
37	0.0	0.0	0.0	6.159989682399416	0.0
38	0.0	0.0	0.0	6.300355452258238	0.0
39	0.0	0.0	0.0	6.498518892058928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	40	6.8175723E-9	45.0	1
TAATACG	40	6.8175723E-9	45.0	1
ATCTCGA	20	7.033924E-4	45.0	37
ACACGTA	20	7.033924E-4	45.0	24
CGTTGTA	20	7.033924E-4	45.0	43
GTAATAG	20	7.033924E-4	45.0	2
CTCGGTA	20	7.033924E-4	45.0	17
CATAGAT	20	7.033924E-4	45.0	41
AACGCGT	35	1.2122291E-7	45.0	30
GGTATAC	20	7.033924E-4	45.0	26
CGAATCA	35	1.2122291E-7	45.0	27
GTATCGA	20	7.033924E-4	45.0	28
CGTCGCA	40	6.8175723E-9	45.0	18
ACGAGTT	20	7.033924E-4	45.0	24
TAATGCG	80	0.0	45.0	1
TCGGTAC	50	2.1827873E-11	45.0	36
GCGTAAG	25	3.8913327E-5	45.0	1
AATCGCG	25	3.8913327E-5	45.0	1
TACGTAA	20	7.033924E-4	45.0	40
CTTACGT	20	7.033924E-4	45.0	38
>>END_MODULE