##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2172190
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14489984761922	31.0	31.0	33.0	30.0	34.0
2	31.47885866337659	31.0	31.0	34.0	30.0	34.0
3	31.521436890879713	31.0	31.0	34.0	30.0	34.0
4	35.36433737380248	37.0	35.0	37.0	33.0	37.0
5	35.27782330274976	37.0	35.0	37.0	33.0	37.0
6	35.33065845989531	37.0	35.0	37.0	33.0	37.0
7	35.80690363181858	37.0	35.0	37.0	35.0	37.0
8	35.72763017968042	37.0	35.0	37.0	35.0	37.0
9	37.483174123810535	39.0	37.0	39.0	35.0	39.0
10	36.97092749713423	39.0	37.0	39.0	32.0	39.0
11	36.6491830825112	39.0	35.0	39.0	32.0	39.0
12	35.72017825328355	37.0	35.0	39.0	31.0	39.0
13	35.320814017190024	37.0	35.0	39.0	30.0	39.0
14	36.16280251727519	38.0	35.0	40.0	30.0	41.0
15	36.51121725079298	38.0	35.0	40.0	31.0	41.0
16	36.656740432466776	38.0	35.0	40.0	32.0	41.0
17	36.59822161044844	38.0	35.0	40.0	32.0	41.0
18	36.512193224349616	38.0	35.0	40.0	31.0	41.0
19	36.37014579755915	37.0	35.0	40.0	31.0	41.0
20	36.20949962940627	37.0	35.0	40.0	31.0	41.0
21	36.02275215335675	37.0	34.0	40.0	31.0	41.0
22	35.88775889770232	36.0	34.0	40.0	30.0	41.0
23	35.8549127838725	36.0	34.0	40.0	30.0	41.0
24	35.83524829780084	36.0	34.0	40.0	30.0	41.0
25	35.76588880346563	36.0	34.0	40.0	30.0	41.0
26	35.641959957462284	36.0	34.0	40.0	30.0	41.0
27	35.51808267232609	36.0	34.0	40.0	30.0	41.0
28	35.46261606949668	36.0	34.0	40.0	30.0	41.0
29	35.42576570189532	36.0	34.0	40.0	30.0	41.0
30	35.25609408016794	36.0	34.0	40.0	29.0	41.0
31	35.00096630589405	36.0	34.0	40.0	28.0	41.0
32	34.75549974910114	35.0	34.0	40.0	27.0	41.0
33	34.47260967042478	35.0	33.0	40.0	25.0	41.0
34	34.22242713574779	35.0	33.0	40.0	24.0	41.0
35	33.980204309936056	35.0	33.0	40.0	23.0	41.0
36	33.717608036129434	35.0	33.0	40.0	22.0	41.0
37	33.53093513919132	35.0	33.0	40.0	22.0	41.0
38	33.46507902163255	35.0	32.0	40.0	22.0	41.0
39	33.46652917102095	35.0	33.0	40.0	22.0	41.0
40	33.232594754602495	35.0	32.0	39.0	21.0	41.0
41	33.165172936069126	35.0	32.0	39.0	20.0	41.0
42	33.024259848355804	35.0	32.0	39.0	19.0	41.0
43	32.93437268378917	35.0	32.0	39.0	19.0	41.0
44	32.746536905151025	35.0	31.0	39.0	18.0	41.0
45	32.71677109276813	35.0	31.0	39.0	19.0	41.0
46	32.59039678849456	35.0	31.0	39.0	18.0	41.0
47	32.54340274101253	35.0	31.0	39.0	18.0	41.0
48	32.50316592931558	35.0	31.0	39.0	18.0	41.0
49	32.50647825466465	35.0	31.0	38.0	18.0	40.0
50	32.34904727487006	35.0	31.0	38.0	18.0	40.0
51	32.11971742803346	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	9.0
13	10.0
14	38.0
15	99.0
16	271.0
17	820.0
18	1735.0
19	3208.0
20	5379.0
21	8628.0
22	13233.0
23	18954.0
24	26806.0
25	37240.0
26	46968.0
27	53421.0
28	56548.0
29	61457.0
30	70473.0
31	83092.0
32	102341.0
33	132628.0
34	228041.0
35	317615.0
36	150131.0
37	181290.0
38	248534.0
39	323145.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.753713993711415	23.783370699616516	29.000824053144527	15.462091253527547
2	31.34831667579724	26.349720788697123	30.02462952135863	12.277333014147013
3	28.59418375004028	24.938840525000114	33.394086152684615	13.072889572274985
4	25.761282392424235	27.227820770742888	31.872303988141	15.138592848691873
5	21.65183524461488	32.649031622463966	31.659016936824123	14.040116196097028
6	19.450554509504233	41.434220763377056	28.299642296484194	10.815582430634521
7	85.53031732951537	4.645726202588171	8.259084150097367	1.5648723177990875
8	86.44994222420691	3.852287322932156	7.408836243606682	2.288934209254255
9	82.84473273516589	4.948692333543566	9.51868851251502	2.6878864187755216
10	51.61201368204439	23.314765282963275	14.117319387346411	10.955901647645923
11	45.05347138141691	20.556350963773887	20.910049305079205	13.480128349729997
12	40.744271909915796	19.74845662672234	25.414443487908517	14.092827975453345
13	23.26453947398708	37.17685837795036	24.888936971443563	14.669665176618988
14	15.730944346489025	41.3119478498658	27.66995520649667	15.287152597148502
15	14.546149277917678	22.71707355249771	47.89567210971416	14.841105059870454
16	16.27859441393248	17.37596619080283	45.7215989393193	20.623840455945384
17	16.367214654335026	18.074615940594516	30.413868031802004	35.144301373268455
18	21.942463596646704	21.40719734461534	34.64618656747338	22.004152491264577
19	27.374170767750517	25.102684387645645	26.68500453459412	20.838140310009713
20	30.11978694313113	22.68484801053315	25.655812797223078	21.53955224911265
21	22.913188993596325	27.464632467693896	28.396595141309	21.22558339740078
22	23.00319032865449	24.256809947564438	26.005137672118924	26.734862051662144
23	19.589584704837055	29.663519305401458	24.483677762994947	26.263218226766533
24	20.86774177212859	23.694474240282847	36.90413821995314	18.533645767635427
25	18.204945239596903	24.076899350425148	34.47796923841837	23.24018617155958
26	17.474668422191428	31.57541467367035	28.55086341434221	22.39905348979601
27	18.56260271891501	30.85816618251626	29.6622763202114	20.916954778357326
28	16.468264746638187	27.029863870103444	37.90211721810707	18.5997541651513
29	17.11512344684397	23.926083814031003	37.22073115151069	21.738061587614343
30	20.384036387240524	28.366855569724564	32.06841942923961	19.180688613795294
31	29.28321187373112	25.301746164009593	24.403666345945794	21.01137561631349
32	30.30043412408675	26.210552483898734	25.02815131273047	18.46086207928404
33	28.12590058880669	26.010385831810297	25.76257141410282	20.1011421652802
34	21.104875724499237	26.121702061053593	28.931401028455156	23.842021185992017
35	21.55801288100949	24.284339767699876	31.010685068985676	23.146962282304955
36	30.64469498524531	22.91760849649432	27.13749718026508	19.300199337995295
37	22.534676984978294	29.012056956343596	29.24242354490169	19.21084251377642
38	22.01469484713584	30.985595182741843	24.170123239679768	22.82958673044255
39	21.4071052716383	29.1869035397456	27.322701973584262	22.083289215031833
40	23.31540979380257	24.35624876276937	26.972410332429487	25.355931110998576
41	18.346323295844286	22.375160552253718	28.615636753690975	30.662879398211025
42	22.895833237423982	24.563320888135937	24.481652157500033	28.059193716940044
43	23.289122958857188	25.056417716682244	26.496439077612916	25.158020246847652
44	21.00681800394993	28.556571938918786	27.194720535496437	23.24188952163485
45	19.596075849718485	33.21624719752876	24.28512238800473	22.90255456474802
46	23.499003310023525	29.930208683402466	26.931115602226324	19.639672404347685
47	22.380178529502484	26.16143155064704	28.2000653718137	23.258324548036775
48	23.870057407501186	22.395738862622512	29.9975600661084	23.736643663767904
49	20.96317541283221	22.255143426679986	32.333773749073515	24.44790741141429
50	20.040512109898305	28.865614886358927	28.48889830079321	22.60497470294956
51	19.414507938992447	31.127617749828513	24.745165017793102	24.712709293385938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1294.0
1	1877.0
2	2460.0
3	11863.5
4	21267.0
5	15784.5
6	10302.0
7	10907.5
8	11513.0
9	12273.0
10	13033.0
11	13236.0
12	13439.0
13	13014.0
14	12589.0
15	11997.5
16	11406.0
17	10706.0
18	10006.0
19	9824.0
20	9642.0
21	9664.5
22	9687.0
23	9305.0
24	8923.0
25	8844.0
26	10857.5
27	12950.0
28	14231.0
29	15512.0
30	19462.0
31	23412.0
32	27007.5
33	30603.0
34	36486.5
35	42370.0
36	44263.0
37	46156.0
38	58223.5
39	70291.0
40	101303.5
41	132316.0
42	162934.0
43	193552.0
44	200923.0
45	208294.0
46	205684.0
47	203074.0
48	192986.5
49	182899.0
50	177622.0
51	172345.0
52	157243.0
53	142141.0
54	125220.5
55	108300.0
56	101232.0
57	94164.0
58	91115.0
59	88066.0
60	84806.5
61	81547.0
62	71035.5
63	60524.0
64	51897.5
65	43271.0
66	35459.0
67	27647.0
68	22596.5
69	17546.0
70	14526.0
71	11506.0
72	10046.5
73	8587.0
74	6572.0
75	3405.5
76	2254.0
77	2170.0
78	2086.0
79	1467.5
80	849.0
81	777.5
82	706.0
83	441.5
84	177.0
85	132.5
86	88.0
87	64.0
88	40.0
89	28.0
90	16.0
91	15.0
92	14.0
93	9.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2172190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.459661284453098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34742333567144	16.848003890953066
2	7.484294805074218	3.0625227332962086
3	2.5526873778037347	1.5668135734488948
4	1.2028687662189956	0.9844115010595461
5	0.7357342053771745	0.752643631870162
6	0.49967495946608864	0.6133908253799404
7	0.3630625010553439	0.5199695057675112
8	0.29462403446797025	0.4822326361178965
9	0.22537842500454142	0.41500496127747893
>10	2.365473764666948	10.889616043706612
>50	0.6784028208693738	10.064418817003785
>100	1.2361430937203248	50.70572773193015
>500	0.011521070488801884	1.5431619349210768
>1k	0.00225903342917684	0.5396075113874318
>5k	2.25903342917684E-4	0.4519299568604975
>10k+	2.25903342917684E-4	0.5605447450198521
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12128	0.5583305327802817	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9778	0.45014478475639796	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10952080619098697	0.0
2	0.0	0.0	0.0	0.325293827887984	0.0
3	0.0	0.0	0.0	0.4286917811057044	0.0
4	0.0	0.0	0.0	0.5971392926033174	0.0
5	0.0	0.0	0.0	0.9345407169722723	0.0
6	0.0	0.0	0.0	1.3019579318567898	0.0
7	0.0	0.0	0.0	1.5628006758156514	0.0
8	0.0	0.0	0.0	2.352556636390003	0.0
9	0.0	0.0	0.0	2.7802816512367703	0.0
10	0.0	0.0	0.0	3.5057246373475617	0.0
11	0.0	0.0	0.0	4.033164686330386	0.0
12	0.0	0.0	0.0	4.54688586173401	0.0
13	0.0	0.0	0.0	4.746454039471685	0.0
14	0.0	0.0	0.0	4.813575239735014	0.0
15	0.0	0.0	0.0	4.918492397073921	0.0
16	0.0	0.0	0.0	5.146695270671534	0.0
17	0.0	0.0	0.0	5.419277319203201	0.0
18	0.0	0.0	0.0	5.892210165777395	0.0
19	0.0	0.0	0.0	6.052463182318306	0.0
20	0.0	0.0	0.0	6.251064593797044	0.0
21	0.0	0.0	0.0	6.433967562690189	0.0
22	0.0	0.0	0.0	6.621520216923934	0.0
23	0.0	0.0	0.0	6.831860932975476	0.0
24	0.0	0.0	0.0	6.990318526464075	0.0
25	4.603648852080159E-5	0.0	0.0	7.109599068221472	0.0
26	4.603648852080159E-5	0.0	0.0	7.231457653336034	0.0
27	4.603648852080159E-5	0.0	0.0	7.3657000538626916	0.0
28	4.603648852080159E-5	0.0	0.0	7.493727528439041	0.0
29	4.603648852080159E-5	0.0	0.0	7.637499482089504	0.0
30	4.603648852080159E-5	0.0	0.0	7.826893595864082	0.0
31	4.603648852080159E-5	0.0	0.0	7.991243859883344	0.0
32	4.603648852080159E-5	0.0	0.0	8.138836842081034	0.0
33	4.603648852080159E-5	0.0	0.0	8.280399044282499	0.0
34	4.603648852080159E-5	0.0	0.0	8.433746587545288	0.0
35	4.603648852080159E-5	0.0	0.0	8.618260833536661	0.0
36	4.603648852080159E-5	0.0	0.0	8.78182847725107	0.0
37	4.603648852080159E-5	0.0	0.0	8.941022654556	0.0
38	4.603648852080159E-5	4.603648852080159E-5	0.0	9.101966218424723	0.0
39	4.603648852080159E-5	4.603648852080159E-5	0.0	9.267007029771797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAG	90	0.0	45.000004	1
TAATACG	75	0.0	45.0	1
CGTATAT	25	3.891957E-5	45.0	14
GCGCCTA	20	7.034675E-4	45.0	27
TACGGTG	20	7.034675E-4	45.0	29
CGCGAAA	25	3.891957E-5	45.0	37
CGTAGTC	25	3.891957E-5	45.0	13
TGCGAAC	35	1.2125929E-7	45.0	10
TATTCGA	35	1.2125929E-7	45.0	39
TCGCTAA	50	2.1827873E-11	45.0	15
TCGTCAG	25	3.891957E-5	45.0	30
ATGCGTA	25	3.891957E-5	45.0	34
CGTTTCA	40	6.8193913E-9	45.0	30
ACGACGT	40	6.8193913E-9	45.0	21
AGTCGTT	20	7.034675E-4	45.0	18
TAACTAC	25	3.891957E-5	45.0	10
CGAAGTA	35	1.2125929E-7	45.0	43
TACAACG	20	7.034675E-4	45.0	14
CGGACGT	20	7.034675E-4	45.0	33
CCGCTTA	30	2.1663982E-6	44.999996	41
>>END_MODULE