##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	650332
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78183297146688	31.0	31.0	33.0	30.0	34.0
2	32.20086048356839	33.0	31.0	34.0	31.0	34.0
3	32.26730346961244	33.0	31.0	34.0	31.0	34.0
4	36.056282022105634	37.0	35.0	37.0	35.0	37.0
5	36.041998548433725	37.0	35.0	37.0	35.0	37.0
6	36.11420782000578	37.0	35.0	37.0	35.0	37.0
7	36.15158103860797	37.0	35.0	37.0	35.0	37.0
8	36.0211722627827	37.0	35.0	37.0	35.0	37.0
9	37.87104740348007	39.0	38.0	39.0	35.0	39.0
10	37.730408468290044	39.0	37.0	39.0	35.0	39.0
11	37.54780635121753	39.0	37.0	39.0	35.0	39.0
12	36.88395773235824	39.0	35.0	39.0	34.0	39.0
13	36.6358706014774	39.0	35.0	39.0	33.0	39.0
14	37.77954490936937	40.0	35.0	41.0	33.0	41.0
15	37.88856768542836	40.0	35.0	41.0	33.0	41.0
16	37.99811019602296	40.0	35.0	41.0	34.0	41.0
17	37.861544564929915	40.0	35.0	41.0	34.0	41.0
18	37.79294268158417	39.0	36.0	41.0	34.0	41.0
19	37.65425813276911	39.0	36.0	41.0	34.0	41.0
20	37.40240061999102	39.0	35.0	41.0	34.0	41.0
21	37.36907456499142	39.0	35.0	41.0	34.0	41.0
22	37.26781551576733	39.0	35.0	41.0	33.0	41.0
23	37.156037224064015	38.0	35.0	41.0	33.0	41.0
24	37.207232613495876	38.0	35.0	41.0	33.0	41.0
25	37.19744684253581	38.0	35.0	41.0	33.0	41.0
26	37.11245179385298	38.0	35.0	41.0	33.0	41.0
27	36.9898774779651	38.0	35.0	41.0	33.0	41.0
28	36.94054421433975	38.0	35.0	41.0	33.0	41.0
29	36.85627802414766	38.0	35.0	41.0	33.0	41.0
30	36.75149923423728	38.0	35.0	41.0	33.0	41.0
31	36.55479047624905	38.0	35.0	41.0	33.0	41.0
32	36.24957560138514	38.0	35.0	41.0	32.0	41.0
33	35.98249355713697	38.0	35.0	41.0	31.0	41.0
34	35.67122638898286	38.0	35.0	41.0	30.0	41.0
35	35.41212949693387	38.0	35.0	40.0	29.0	41.0
36	35.22805428611847	38.0	35.0	40.0	27.0	41.0
37	35.07438200795901	37.0	35.0	40.0	26.0	41.0
38	34.981512519759136	37.0	35.0	40.0	26.0	41.0
39	34.95133255014362	37.0	35.0	40.0	26.0	41.0
40	34.70915470867188	37.0	34.0	40.0	24.0	41.0
41	34.6437019860625	36.0	34.0	40.0	24.0	41.0
42	34.5440590344624	36.0	34.0	40.0	23.0	41.0
43	34.469629358543024	36.0	34.0	40.0	23.0	41.0
44	34.278087192387886	36.0	34.0	40.0	23.0	41.0
45	34.21772571548071	35.0	34.0	40.0	23.0	41.0
46	34.1277116918743	35.0	34.0	40.0	23.0	41.0
47	34.056872489743704	35.0	34.0	40.0	23.0	41.0
48	34.03650135623035	35.0	34.0	40.0	23.0	41.0
49	34.03278479299804	35.0	34.0	40.0	23.0	41.0
50	33.87072756684278	35.0	34.0	40.0	23.0	41.0
51	33.715918946015265	35.0	34.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	1.0
14	9.0
15	12.0
16	33.0
17	112.0
18	280.0
19	456.0
20	778.0
21	1458.0
22	2346.0
23	3446.0
24	5152.0
25	7684.0
26	10416.0
27	11735.0
28	10949.0
29	10382.0
30	10839.0
31	13171.0
32	17299.0
33	25062.0
34	58018.0
35	100633.0
36	42865.0
37	59781.0
38	96000.0
39	161366.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.05896065394291	22.834951993750884	28.085500944133152	16.020586408173056
2	33.072030901139726	26.422350430241785	28.16515256822669	12.3404661003918
3	27.539625914148463	26.58780438299207	33.364189367892095	12.50838033496737
4	26.12465632938253	25.886162759944153	33.82579974536083	14.163381165312487
5	23.842283633590228	30.803958593456883	30.92128328300007	14.432474489952824
6	19.7543716132683	41.64964971737513	28.33675722554019	10.25922144381639
7	82.34824674166425	4.708518110749586	11.206276178936298	1.7369589686498588
8	83.76952079860747	5.322204658543636	8.967265950314609	1.9410085925342748
9	79.85567371742434	4.971614498440796	10.60366089935602	4.569050884778851
10	49.278061051893495	21.96401222760067	16.04026866277532	12.717658057730514
11	42.485838002743215	20.083126772171752	22.589231346450735	14.841803878634297
12	36.976805693092146	19.665340164715868	27.4073857660395	15.950468376152488
13	22.994255241937964	33.490586346666014	28.07858140149954	15.436577009896485
14	17.081121642484145	36.953279248137875	29.738810330723386	16.226788778654594
15	15.29065154413438	21.829619332894584	45.79691603673201	17.082813086239028
16	17.23642693270514	16.817871487178856	42.81659214062971	23.129109439486292
17	17.487990749340337	18.5580595757244	31.269105626049466	32.6848440488858
18	21.759193765645854	22.148687132110982	34.17254571511167	21.919573387131496
19	27.977556079048853	23.51475861559942	26.563816635195565	21.943868670156167
20	29.87304945781539	21.705528868331868	27.1659706119336	21.255451061919143
21	23.648690207463265	26.692366360566606	28.886783981105037	20.772159450865097
22	22.483592995577645	22.160681006009238	27.288523400355512	28.067202598057605
23	21.02003284476237	28.02414766611515	26.262278343984303	24.693541145138177
24	21.423057761266552	22.390717356673207	36.235030722769295	19.951194159290946
25	20.000399795796607	24.49087542978048	33.44983792893476	22.05888684548815
26	18.314952977863612	28.496675544183585	29.270587945849197	23.917783532103602
27	19.421618496398764	28.569561393257597	30.233173209991204	21.775646900352434
28	16.83909141792192	26.374990005105087	37.46763191723612	19.31828665973687
29	18.779331172385795	23.328084732106063	35.96762884188384	21.924955253624304
30	20.22597688565225	28.411026983140918	31.789608999710918	19.573387131495913
31	28.19805883763985	25.528653057207702	24.806252806258957	21.46703529889349
32	28.646906503139935	25.659509296789945	26.48016705313594	19.21341714693418
33	26.88165429349932	26.397901379603034	25.74869451295646	20.971749813941187
34	20.30378329837683	26.222913834779778	28.87217605776742	24.60112680907598
35	21.263908280693553	25.06627384166856	30.14137394438533	23.528443933252554
36	28.347674726139882	26.645005935429904	25.381343682918878	19.625975655511336
37	21.867907468800553	30.434762552050337	26.72296611576856	20.97436386338055
38	22.341050417325302	30.73261042052367	24.09077209794382	22.835567064207204
39	21.576979142960827	28.486834416882456	26.052846853607083	23.88333958654964
40	24.334801301489087	24.256072283080027	26.698978367972053	24.710148047458837
41	18.811468603728557	24.522551558281	27.320507064084193	29.345472773906252
42	22.059809451172633	25.78867409261731	24.740440267432636	27.41107618877742
43	22.4326959153171	25.81973515066151	25.78759771931875	25.959971214702644
44	21.010191717461236	26.75556484995356	29.082068850986882	23.152174581598324
45	20.178770228129633	32.01134189921456	24.638492339297464	23.171395533358346
46	23.009478235731905	29.937170552886833	26.8041554160029	20.249195795378363
47	22.787745336228266	26.38006433636973	27.59006169156677	23.242128635835236
48	22.331363057638253	25.234034308630054	29.24121833155988	23.19338430217181
49	21.054630557930412	23.59610168344784	31.968133199658023	23.38113455896373
50	20.722646279131276	28.072738232164497	28.035218934328928	23.169396554375304
51	20.109882337021705	29.427123376982834	26.211996334180082	24.250997951815382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	742.5
2	1084.0
3	5793.5
4	10503.0
5	7491.0
6	4479.0
7	4571.5
8	4664.0
9	4699.5
10	4735.0
11	4675.0
12	4615.0
13	4361.5
14	4108.0
15	3883.5
16	3659.0
17	3375.5
18	3092.0
19	2904.0
20	2716.0
21	2644.0
22	2572.0
23	2697.0
24	2822.0
25	2908.5
26	3735.0
27	4475.0
28	4893.5
29	5312.0
30	6452.0
31	7592.0
32	9191.5
33	10791.0
34	12689.5
35	14588.0
36	14961.5
37	15335.0
38	18318.5
39	21302.0
40	27960.5
41	34619.0
42	41089.5
43	47560.0
44	48706.5
45	49853.0
46	53137.5
47	56422.0
48	52286.0
49	48150.0
50	46320.5
51	44491.0
52	42314.5
53	40138.0
54	37387.0
55	34636.0
56	32769.0
57	30902.0
58	30165.0
59	29428.0
60	28178.5
61	26929.0
62	25101.5
63	23274.0
64	19889.5
65	16505.0
66	14260.0
67	12015.0
68	10271.0
69	8527.0
70	7122.5
71	5718.0
72	4843.5
73	3969.0
74	3285.5
75	2086.5
76	1571.0
77	1082.5
78	594.0
79	434.5
80	275.0
81	222.0
82	169.0
83	121.5
84	74.0
85	52.5
86	31.0
87	20.5
88	10.0
89	10.5
90	11.0
91	13.5
92	16.0
93	8.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	650332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.55061633352053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8722064089448	16.566434270303677
2	6.286485351262596	2.709552677827145
3	2.520113302183463	1.629299846770719
4	1.4704020930010584	1.2675228544908554
5	1.050596573971962	1.1320501843490438
6	0.8230935920852509	1.0642904525765096
7	0.7554413606975866	1.139615884880647
8	0.5677343618345858	0.9788020329002624
9	0.5125872487335091	0.9941914021419642
>10	7.136940761176827	38.00978642235969
>50	1.8321235730655452	26.600067619802797
>100	0.16512701731492038	5.417990259966783
>500	0.003574177863959315	0.47247586372812406
>1k	0.0028593422911674525	1.1613940452058453
>5k	7.148355727918631E-4	0.8565261826959145
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5560	0.8549479342858725	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCG	2621	0.4030249165041856	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2161	0.33229181402729685	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGC	1723	0.26494159906017234	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTC	1034	0.15899571295891946	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	738	0.11348049919118235	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	682	0.10486951280269155	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03321380464132166	0.0
2	0.0	0.0	0.0	0.15622789590547598	0.0
3	0.0	0.0	0.0	0.22619216031196374	0.0
4	0.0	0.0	0.0	0.39595160625649667	0.0
5	0.0	0.0	0.0	0.766839091417922	0.0
6	0.0	0.0	0.0	0.993338786958046	0.0
7	0.0	0.0	0.0	1.1801664380654804	0.0
8	0.0	0.0	0.0	1.5702748749869297	0.0
9	0.0	0.0	0.0	1.7417257646863449	0.0
10	0.0	0.0	0.0	2.458590381528204	0.0
11	0.0	0.0	0.0	2.7050798668987532	0.0
12	0.0	0.0	0.0	3.1986739080961724	0.0
13	0.0	0.0	0.0	3.320150323219525	0.0
14	0.0	0.0	0.0	3.3739689881475923	0.0
15	0.0	0.0	0.0	3.479607339020685	0.0
16	0.0	0.0	0.0	3.624917734326467	0.0
17	0.0	0.0	0.0	3.7614633756296785	0.0
18	0.0	0.0	0.0	4.018870361599921	0.0
19	0.0	0.0	0.0	4.171254067153392	0.0
20	0.0	0.0	0.0	4.300265095366674	0.0
21	0.0	0.0	0.0	4.40820996045097	0.0
22	0.0	0.0	0.0	4.518307572132388	0.0
23	0.0	0.0	0.0	4.6400915224839006	0.0
24	0.0	0.0	0.0	4.726662689211049	0.0
25	0.0	0.0	0.0	4.800009841127301	0.0
26	0.0	0.0	0.0	4.87105047883235	0.0
27	0.0	0.0	0.0	4.9565452722609376	0.0
28	0.0	0.0	0.0	5.032660241230633	0.0
29	0.0	0.0	0.0	5.114310844307216	0.0
30	0.0	0.0	0.0	5.228560181568799	0.0
31	0.0	0.0	0.0	5.3097494818031405	0.0
32	0.0	0.0	0.0	5.39708948660069	0.0
33	0.0	0.0	0.0	5.490887731189607	0.0
34	0.0	0.0	0.0	5.582686996795483	0.0
35	0.0	0.0	0.0	5.714465842062208	0.0
36	0.0	0.0	0.0	5.812415812231291	0.0
37	0.0	0.0	0.0	5.909904479558134	0.0
38	0.0	0.0	0.0	6.006931844042735	0.0
39	0.0	0.0	0.0	6.10626572273854	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	55	1.8189894E-12	45.0	25
CTCACGT	55	1.8189894E-12	45.0	45
GATAATC	20	7.031025E-4	45.0	9
GGTCGAG	20	7.031025E-4	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
CTATCGC	25	3.888931E-5	45.0	44
TAGACCC	20	7.031025E-4	45.0	39
AACCACG	25	3.888931E-5	45.0	33
TAAACGG	40	6.8066583E-9	45.0	2
CACGACC	25	3.888931E-5	45.0	27
ACGGGTA	100	0.0	45.0	5
TCGGCGA	20	7.031025E-4	45.0	23
ACTAGGT	20	7.031025E-4	45.0	3
CTTGCGA	20	7.031025E-4	45.0	13
TCTCGAG	25	3.888931E-5	45.0	1
CGAATTA	20	7.031025E-4	45.0	44
ATGGTCG	40	6.8066583E-9	45.0	44
GTATATC	20	7.031025E-4	45.0	24
CTCGAGG	40	6.8066583E-9	45.0	2
GGTCTAA	55	1.8189894E-12	45.0	32
>>END_MODULE