##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	676081
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30992588166211	31.0	31.0	33.0	30.0	34.0
2	31.665010553469184	31.0	31.0	34.0	30.0	34.0
3	31.766712864286973	31.0	31.0	34.0	30.0	34.0
4	35.599302450446025	37.0	35.0	37.0	33.0	37.0
5	35.52022612675108	37.0	35.0	37.0	33.0	37.0
6	35.554970484305876	37.0	35.0	37.0	33.0	37.0
7	35.86648345390567	37.0	35.0	37.0	35.0	37.0
8	35.780393177740535	37.0	35.0	37.0	35.0	37.0
9	37.549292170612695	39.0	37.0	39.0	35.0	39.0
10	37.1724393970545	39.0	37.0	39.0	33.0	39.0
11	36.86882488932539	39.0	37.0	39.0	33.0	39.0
12	35.910667508774836	37.0	35.0	39.0	32.0	39.0
13	35.47549480017927	37.0	35.0	39.0	31.0	39.0
14	36.36469446708308	38.0	35.0	40.0	31.0	41.0
15	36.70146624442929	38.0	35.0	41.0	32.0	41.0
16	36.88682273277906	38.0	35.0	41.0	32.0	41.0
17	36.85270847723867	38.0	35.0	41.0	32.0	41.0
18	36.78123331375974	38.0	35.0	40.0	32.0	41.0
19	36.67579328512412	37.0	35.0	40.0	32.0	41.0
20	36.515327305456005	37.0	35.0	40.0	32.0	41.0
21	36.358572419576944	37.0	35.0	40.0	31.0	41.0
22	36.26916893094171	37.0	35.0	40.0	31.0	41.0
23	36.228800986864	37.0	35.0	40.0	31.0	41.0
24	36.21036088871008	37.0	35.0	40.0	31.0	41.0
25	36.15369016434421	37.0	35.0	40.0	31.0	41.0
26	36.051706822111555	37.0	35.0	40.0	31.0	41.0
27	35.927066431389136	36.0	35.0	40.0	31.0	41.0
28	35.939170010693985	36.0	35.0	40.0	31.0	41.0
29	35.92393514978235	36.0	35.0	40.0	31.0	41.0
30	35.83786853942057	36.0	35.0	40.0	30.0	41.0
31	35.639200036682	36.0	34.0	40.0	30.0	41.0
32	35.45764043065846	36.0	34.0	40.0	30.0	41.0
33	35.26490908633729	36.0	34.0	40.0	29.0	41.0
34	35.07447036671641	36.0	34.0	40.0	28.0	41.0
35	34.89286490819887	36.0	34.0	40.0	27.0	41.0
36	34.69121155601178	36.0	34.0	40.0	26.0	41.0
37	34.46139293960339	35.0	33.0	40.0	25.0	41.0
38	34.457177468380266	35.0	33.0	40.0	26.0	41.0
39	34.417131970873314	35.0	33.0	40.0	25.0	41.0
40	34.19573542223491	35.0	33.0	40.0	23.0	41.0
41	34.15725630508771	35.0	33.0	40.0	23.0	41.0
42	34.085517859546414	35.0	33.0	40.0	23.0	41.0
43	34.05799009290307	35.0	33.0	40.0	24.0	41.0
44	33.8879379837623	35.0	33.0	40.0	23.0	41.0
45	33.833210813497196	35.0	33.0	39.0	23.0	41.0
46	33.779168472416764	35.0	33.0	39.0	23.0	41.0
47	33.74148659702018	35.0	33.0	39.0	23.0	41.0
48	33.72169754807486	35.0	33.0	39.0	23.0	41.0
49	33.73740128771553	35.0	33.0	39.0	24.0	41.0
50	33.61938880104603	35.0	33.0	39.0	24.0	41.0
51	33.41032361506979	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	5.0
14	9.0
15	20.0
16	56.0
17	128.0
18	325.0
19	655.0
20	1113.0
21	1819.0
22	2649.0
23	3884.0
24	5694.0
25	8258.0
26	10757.0
27	12478.0
28	13420.0
29	15287.0
30	18172.0
31	22771.0
32	29479.0
33	39680.0
34	73837.0
35	107313.0
36	46883.0
37	58557.0
38	83579.0
39	119217.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.721642820904595	24.07670086868289	28.81636963618265	15.385286674229862
2	29.02936186640358	27.289037851973358	30.588050840062063	13.093549441560997
3	28.016909216499208	25.152903276382567	32.93910049239662	13.89108701472161
4	25.787886362728724	27.688842017450572	31.47995580411223	15.043315815708471
5	22.493162801498638	31.709809919225656	30.968034895227053	14.828992384048655
6	20.939946544866668	40.17684271559177	28.06941771769951	10.813793021842057
7	85.8931104409087	5.238869307080068	6.806285045726769	2.061735206284454
8	87.94597097093396	4.07865329745992	6.050606362255411	1.924769369350714
9	83.56409956795117	5.249371007320129	8.11248947981085	3.0740399449178426
10	54.52320062241063	21.708049775100914	13.143247628612547	10.625501973875911
11	49.208452833314354	18.925395034026987	18.9006938517722	12.96545828088646
12	44.268955938711485	19.786978187524866	22.91308289983005	13.030982973933597
13	22.652167417809405	41.57120226718396	22.069544921392556	13.70708539361408
14	15.500509554328548	43.63352911855236	25.657872355531364	15.208088971587724
15	12.909695731724453	22.035821151607575	50.261728994011065	14.7927541226569
16	14.69646388524452	16.550531667063563	48.213453713386414	20.539550734305507
17	15.70270426176745	17.46417958794878	28.500283250083942	38.332832900199826
18	21.95417413002288	22.691807638433854	33.36035179216692	21.993666439376348
19	30.229366007919168	24.370304741591614	23.822885127669615	21.577444122819603
20	33.234183478015204	21.67299480387705	23.27635298137353	21.81646873673421
21	22.06969283266354	27.90464456182026	25.972035895107243	24.05362671040896
22	22.55247522116433	25.399471365117492	23.97375462407611	28.07429878964207
23	20.370044417754677	30.53243620217104	23.451627837492843	25.645891542581435
24	21.21313866237921	23.26422425715262	37.249087017679834	18.273550062788335
25	17.44243663111373	25.548713837543136	34.274295535594106	22.734553995749028
26	17.208441000412673	33.75808519985031	27.071904106164794	21.96156969357222
27	18.305498897321478	32.01495087127134	28.86591991196321	20.81363031944397
28	15.624459199415455	27.160059223672906	38.29274894576242	18.922732631149223
29	16.158270976406676	23.504580072506105	37.860404300668115	22.476744650419107
30	18.596144544810457	28.85364327647131	32.21389153074853	20.336320647969696
31	28.746407605005906	26.562349777615403	23.799958880666665	20.891283736712023
32	30.201114955160698	26.943073389135325	23.514638038933207	19.34117361677077
33	28.11438274407948	28.382693789649466	23.64849773917622	19.854425727094828
34	20.095373187532264	25.7602269550542	29.137485005494906	25.006914851918634
35	20.613950103611845	25.13471019005119	30.47549036284114	23.77584934349582
36	29.489218007901417	24.826315190043797	25.644412429871565	20.040054372183214
37	21.007098261894654	31.596361974378812	28.198100523457985	19.19843924026855
38	21.92281694057369	31.3205074539885	24.213962528158607	22.542713077279203
39	21.38619484943372	31.5886705882875	25.972775451462176	21.052359110816603
40	24.16500389746199	26.202481655304616	24.91106834831921	24.721446098914182
41	17.69802730737885	24.39559756893035	26.56604755939007	31.34032756430073
42	22.597292336273316	27.42629951144907	22.109776787100955	27.86663136517666
43	23.844628084504667	28.076073724893913	23.996680871079057	24.082617319522363
44	21.522569041283514	30.9082787417484	26.19804431717501	21.37110789979307
45	18.016332362542357	36.03103770110386	21.76159365519812	24.191036281155657
46	21.932874907000787	32.75332393603725	25.41633325000998	19.897467906951977
47	22.865899204385272	27.372755631351865	26.87710496227523	22.884240201987634
48	24.328445851902362	22.32735426672248	29.0179726985376	24.32622718283756
49	19.484351727085954	23.527506319509055	32.51947621660718	24.46866573679781
50	18.429300631137394	32.53870468183546	26.83731683037979	22.194677856647356
51	19.087653698299466	33.52556868185913	23.33581331231021	24.050964307531196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	234.0
1	500.5
2	767.0
3	2799.0
4	4831.0
5	3636.0
6	2441.0
7	2569.0
8	2697.0
9	2863.0
10	3029.0
11	3046.0
12	3063.0
13	2851.5
14	2640.0
15	2572.5
16	2505.0
17	2284.0
18	2063.0
19	2105.5
20	2148.0
21	2011.5
22	1875.0
23	2200.5
24	2526.0
25	2929.5
26	3874.5
27	4416.0
28	5503.0
29	6590.0
30	7892.0
31	9194.0
32	10980.5
33	12767.0
34	14751.5
35	16736.0
36	17547.5
37	18359.0
38	22111.5
39	25864.0
40	36421.0
41	46978.0
42	57046.5
43	67115.0
44	64667.5
45	62220.0
46	58890.5
47	55561.0
48	52256.0
49	48951.0
50	49283.5
51	49616.0
52	47051.0
53	44486.0
54	39981.5
55	35477.0
56	32505.5
57	29534.0
58	28200.5
59	26867.0
60	25179.5
61	23492.0
62	21280.5
63	19069.0
64	16398.5
65	13728.0
66	11554.5
67	9381.0
68	7509.5
69	5638.0
70	4780.5
71	3923.0
72	3303.5
73	2684.0
74	2098.5
75	1188.0
76	863.0
77	661.0
78	459.0
79	355.5
80	252.0
81	175.5
82	99.0
83	71.0
84	43.0
85	32.0
86	21.0
87	22.5
88	24.0
89	13.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	676081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.7381369412816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09569388467476	15.612016373613999
2	5.3386091527972255	2.1074839706778197
3	1.8648928352228176	1.1042853048732866
4	1.059044689493947	0.836142764326743
5	0.7341858882310587	0.7245730801130552
6	0.60437966057979	0.7157597103029514
7	0.5019731290503263	0.6935610053427274
8	0.4366135882217092	0.6894351035795545
9	0.39157959420500255	0.6956146488446837
>10	7.2215381735948725	38.165746690112094
>50	2.5932112511943406	33.563368631766096
>100	0.15527761903304269	4.041362190556262
>500	0.0015002668505607988	0.19277719626687134
>1k	0.0015002668505607988	0.8578733296238499
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3003	0.4441775467732416	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2791	0.4128203573240484	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	775	0.11463123501473935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043633824941094336	0.0
2	0.0	0.0	0.0	0.16137119664655566	0.0
3	0.0	0.0	0.0	0.2252688657128362	0.0
4	0.0	0.0	0.0	0.3419708585213902	0.0
5	0.0	0.0	0.0	0.5494903717158152	0.0
6	0.0	0.0	0.0	0.7586369088910944	0.0
7	0.0	0.0	0.0	0.8871718033785893	0.0
8	0.0	0.0	0.0	1.2529563765288478	0.0
9	0.0	0.0	0.0	1.4356267961974971	0.0
10	0.0	0.0	0.0	1.759108745845542	0.0
11	0.0	0.0	0.0	2.0176576475303993	0.0
12	0.0	0.0	0.0	2.2750232590473627	0.0
13	0.0	0.0	0.0	2.361847175116591	0.0
14	0.0	0.0	0.0	2.3911336067719695	0.0
15	0.0	0.0	0.0	2.4443816643272034	0.0
16	0.0	0.0	0.0	2.5486591103728697	0.0
17	0.0	0.0	0.0	2.666396482078331	0.0
18	0.0	0.0	0.0	2.857054110380265	0.0
19	0.0	0.0	0.0	2.927311964098976	0.0
20	0.0	0.0	0.0	3.0131005012712975	0.0
21	0.0	0.0	0.0	3.110869851393546	0.0
22	0.0	0.0	0.0	3.2062726211800063	0.0
23	0.0	0.0	0.0	3.3223829689046136	0.0
24	0.0	0.0	0.0	3.40654448209608	0.0
25	0.0	0.0	0.0	3.475175311833937	0.0
26	0.0	0.0	0.0	3.5433624077588335	0.0
27	0.0	0.0	0.0	3.6109578585997832	0.0
28	0.0	0.0	0.0	3.682694825028362	0.0
29	0.0	0.0	0.0	3.759164952128517	0.0
30	0.0	0.0	0.0	3.8739440984142433	0.0
31	0.0	0.0	0.0	3.964317884987154	0.0
32	0.0	0.0	0.0	4.045521172758885	0.0
33	0.0	0.0	0.0	4.120068453336213	0.0
34	0.0	0.0	0.0	4.202159208733865	0.0
35	0.0	0.0	0.0	4.319748669168339	0.0
36	0.0	0.0	0.0	4.417813841832562	0.0
37	0.0	0.0	0.0	4.515287369412837	0.0
38	0.0	0.0	0.0	4.600188438959237	0.0
39	0.0	0.0	0.0	4.68361039579577	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCCGA	20	7.0312223E-4	45.000004	11
ACACGGC	20	7.0312223E-4	45.000004	28
CGAAATA	25	3.8890947E-5	45.000004	11
CACGGCA	20	7.0312223E-4	45.000004	36
TAACGGT	25	3.8890947E-5	45.000004	36
TAACGGA	20	7.0312223E-4	45.000004	3
TAACGCC	20	7.0312223E-4	45.000004	12
TGCGATG	20	7.0312223E-4	45.000004	1
TCGTCAG	25	3.8890947E-5	45.000004	1
GCGATAC	20	7.0312223E-4	45.000004	9
AACCTAT	25	3.8890947E-5	45.000004	31
ATAACGC	20	7.0312223E-4	45.000004	11
CCGAACT	20	7.0312223E-4	45.000004	39
GCGTGGC	25	3.8890947E-5	45.000004	3
CAACTGA	20	7.0312223E-4	45.000004	41
ATCGGCT	20	7.0312223E-4	45.000004	10
CCCGGTT	25	3.8890947E-5	45.000004	18
CGTTTAG	25	3.8890947E-5	45.000004	30
TTATTAG	40	6.8084773E-9	45.000004	1
TCGAAAT	20	7.0312223E-4	45.000004	17
>>END_MODULE