##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2097900
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195137995137994	31.0	31.0	33.0	30.0	34.0
2	31.536413556413557	31.0	31.0	34.0	30.0	34.0
3	31.5903861003861	31.0	31.0	34.0	30.0	34.0
4	35.42616521283188	37.0	35.0	37.0	33.0	37.0
5	35.330055293388625	37.0	35.0	37.0	33.0	37.0
6	35.370434243767576	37.0	35.0	37.0	33.0	37.0
7	35.81094284760952	37.0	35.0	37.0	35.0	37.0
8	35.733489203489206	37.0	35.0	37.0	35.0	37.0
9	37.478987082320415	39.0	37.0	39.0	35.0	39.0
10	37.045390628723965	39.0	37.0	39.0	33.0	39.0
11	36.66100481433815	39.0	35.0	39.0	32.0	39.0
12	35.50788073788074	37.0	35.0	39.0	31.0	39.0
13	34.962779446112776	37.0	34.0	39.0	30.0	39.0
14	35.797260117260116	37.0	34.0	40.0	29.0	41.0
15	36.22996520329853	37.0	35.0	40.0	31.0	41.0
16	36.42322560655894	37.0	35.0	40.0	32.0	41.0
17	36.394487821154485	37.0	35.0	40.0	32.0	41.0
18	36.29258734925401	37.0	35.0	40.0	31.0	41.0
19	36.146250536250534	37.0	35.0	40.0	31.0	41.0
20	35.96551074884408	36.0	34.0	40.0	31.0	41.0
21	35.78183183183183	36.0	34.0	40.0	30.0	41.0
22	35.64309690643024	35.0	34.0	40.0	30.0	41.0
23	35.64295533628867	35.0	34.0	40.0	30.0	41.0
24	35.58984937318271	35.0	34.0	40.0	30.0	41.0
25	35.51813813813814	35.0	34.0	40.0	30.0	41.0
26	35.38952619285953	35.0	34.0	40.0	30.0	41.0
27	35.262004862004865	35.0	34.0	40.0	29.0	41.0
28	35.25963534963535	36.0	34.0	40.0	29.0	41.0
29	35.261232184565515	36.0	34.0	40.0	29.0	41.0
30	35.14210353210353	36.0	34.0	40.0	29.0	41.0
31	34.85288240621574	35.0	34.0	40.0	29.0	41.0
32	34.611331807998475	35.0	34.0	40.0	27.0	41.0
33	34.386646646646646	35.0	33.0	40.0	25.0	41.0
34	34.176207159540496	35.0	33.0	40.0	24.0	41.0
35	33.954046904046905	35.0	33.0	40.0	23.0	41.0
36	33.70664712331379	35.0	33.0	40.0	23.0	41.0
37	33.490182563515894	35.0	33.0	39.0	23.0	41.0
38	33.4531679298346	35.0	33.0	39.0	23.0	41.0
39	33.444671338004675	35.0	33.0	39.0	23.0	41.0
40	33.19672053005387	35.0	32.0	39.0	21.0	41.0
41	33.14241717908384	35.0	32.0	39.0	21.0	41.0
42	33.04090566757233	35.0	32.0	39.0	21.0	41.0
43	32.95857476524143	35.0	32.0	39.0	21.0	41.0
44	32.79469040469041	35.0	32.0	39.0	20.0	41.0
45	32.7496424996425	35.0	32.0	39.0	20.0	41.0
46	32.68566328232995	35.0	32.0	38.0	20.0	40.0
47	32.6256880690214	35.0	32.0	38.0	20.0	40.0
48	32.57310977644311	35.0	32.0	38.0	20.0	40.0
49	32.616281996282	35.0	32.0	38.0	20.0	40.0
50	32.41495733829067	35.0	31.0	38.0	19.0	40.0
51	32.17909862243196	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	4.0
12	8.0
13	18.0
14	34.0
15	96.0
16	290.0
17	731.0
18	1517.0
19	3024.0
20	5399.0
21	8297.0
22	12398.0
23	17881.0
24	25346.0
25	34867.0
26	44821.0
27	50836.0
28	54056.0
29	58924.0
30	68292.0
31	81872.0
32	101558.0
33	132526.0
34	241478.0
35	343992.0
36	134992.0
37	157068.0
38	219003.0
39	298474.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.472281805615136	23.269126269126268	29.80656847323514	15.452023452023452
2	30.798608131941464	27.312216978883647	29.51618284951618	12.372992039658707
3	28.02945802945803	25.021497688164356	32.83678917012251	14.112255112255113
4	25.617855951189284	27.06778206778207	31.90681157347824	15.407550407550408
5	22.51870918537585	31.71438104771438	31.417036083702747	14.349873683207019
6	21.041422374755708	39.72677439344106	27.940225940225936	11.29157729157729
7	84.34639401306067	5.625387292053959	8.063253729920397	1.9649649649649648
8	86.15343915343915	4.047905047905048	7.526478859812193	2.2721769388436055
9	81.77773011106343	5.419467086133753	9.620811287477954	3.1819915153248486
10	58.05229038562372	18.910529577196243	13.028171028171029	10.00900900900901
11	53.18618618618619	18.179798846465513	17.838886505553173	10.795128461795128
12	45.70375137041804	20.182658849325517	22.13928213928214	11.974307640974308
13	21.521855188521855	43.87301587301587	22.43915343915344	12.165975499308832
14	14.881738881738881	45.39734973068306	27.206015539348872	12.514895848229182
15	13.175699509032842	21.411983411983414	52.998665331998666	12.41365174698508
16	14.782925782925782	16.01086801086801	49.93555460222127	19.270651603984938
17	15.802802802802804	16.519710186376855	29.02717002717003	38.65031698365032
18	22.46003146003146	21.436007436007436	35.50974784308117	20.59421326087993
19	31.049573382906715	22.77796844463511	25.280327946994614	20.89213022546356
20	32.95786262452929	21.71223604556938	24.062920062920064	21.266981266981265
21	22.35769102435769	27.47256780590114	27.67462700796034	22.49511416178083
22	22.866151866151867	24.620954287620954	25.753610753610758	26.759283092616425
23	19.981362314695648	30.11120644453978	24.05457838791172	25.852852852852852
24	21.384813384813384	22.262738929405597	39.54116020782688	16.811287477954146
25	16.769865103198438	23.843462510129175	37.139997139997135	22.246675246675245
26	16.304161304161305	34.8673435340102	27.564373897707227	21.264121264121265
27	18.09957576624243	32.81429048095715	30.25101291767958	18.835120835120836
28	15.08065208065208	27.423661756995088	40.38872205538872	17.106964106964107
29	15.62834262834263	24.00490967157634	38.7366890700224	21.63005863005863
30	19.287239620572954	29.06644739978073	32.22498689165356	19.421326087992753
31	30.787549454216123	24.868535201868536	24.461747461747464	19.88216788216788
32	31.700081033414367	26.234710901377568	25.393870060536727	16.671338004671338
33	28.943800943800944	27.039706373039706	25.12445779112446	18.892034892034893
34	20.00791267457934	26.669336002669336	29.534963534963538	23.78778778778779
35	21.682349015682348	24.81591114924448	31.27846894513561	22.223270889937556
36	31.423471090137756	23.890366557033225	26.107536107536106	18.578626245292913
37	22.144096477429812	31.68320701654035	28.363077363077366	17.809619142952478
38	21.700462367129035	32.36293436293437	22.616425949759282	23.320177320177322
39	22.23823823823824	30.892702226035556	26.160017160017162	20.709042375709043
40	24.64555031221698	25.34863434863435	24.43057343057343	25.57524190857524
41	17.669145335812004	23.41522474855808	26.710901377568042	32.20472853806187
42	23.514514514514513	26.031507698174366	22.986367319700655	27.467610467610466
43	23.746127079460415	26.350398017064684	24.890509557176223	25.01296534629868
44	21.211640211640212	30.827732494399164	26.662900996234328	21.297726297726296
45	18.332379999046665	36.697364030697365	21.934934934934937	23.035321035321036
46	22.844129844129842	31.62424329090996	25.63940130606797	19.892225558892225
47	23.366175699509036	26.003765670432337	27.289622956289623	23.340435673769008
48	25.225892559225894	21.984651317984653	29.987892654559325	22.801563468230135
49	22.02073502073502	21.875017875017875	31.866723866723866	24.237523237523238
50	19.985509318842652	31.891748891748893	26.222412889079553	21.9003289003289
51	18.874207540874206	33.71128271128271	22.96105629438963	24.453453453453452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1396.0
1	2064.0
2	2732.0
3	10889.0
4	19046.0
5	14239.0
6	9432.0
7	10015.0
8	10598.0
9	11448.0
10	12298.0
11	12338.0
12	12378.0
13	11964.0
14	11550.0
15	11002.5
16	10455.0
17	9792.0
18	9129.0
19	8912.5
20	8696.0
21	8805.5
22	8915.0
23	8742.0
24	8569.0
25	9093.5
26	11519.0
27	13420.0
28	14699.5
29	15979.0
30	18801.5
31	21624.0
32	23933.5
33	26243.0
34	29159.0
35	32075.0
36	35647.5
37	39220.0
38	54618.5
39	70017.0
40	107474.0
41	144931.0
42	175627.0
43	206323.0
44	212157.0
45	217991.0
46	205029.0
47	192067.0
48	179969.5
49	167872.0
50	168119.5
51	168367.0
52	157548.5
53	146730.0
54	127198.0
55	107666.0
56	99603.5
57	91541.0
58	83973.5
59	76406.0
60	71998.5
61	67591.0
62	59163.5
63	50736.0
64	43348.0
65	35960.0
66	29487.0
67	23014.0
68	19450.5
69	15887.0
70	14526.0
71	13165.0
72	11380.0
73	9595.0
74	6891.5
75	3232.0
76	2276.0
77	1718.5
78	1161.0
79	902.5
80	644.0
81	399.0
82	154.0
83	136.0
84	118.0
85	86.0
86	54.0
87	39.0
88	24.0
89	19.0
90	14.0
91	18.5
92	23.0
93	15.0
94	7.0
95	5.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2097900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.922457955694302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66983921109457	14.816467174648507
2	7.515594362888633	2.6939584796184928
3	2.408373298719758	1.2949190756376496
4	1.0623303667330726	0.761582853313232
5	0.6599378800166862	0.5913854453984544
6	0.4224333191302593	0.454262604071788
7	0.2941069072701404	0.3689783076019862
8	0.22054192803793463	0.31621227461821133
9	0.18065301669032474	0.29139714865815175
>10	1.8594357962977073	7.866869998511107
>50	0.9366974284316154	12.415207923400004
>100	1.7559045773597373	55.459636443556946
>500	0.01254980461302156	1.4014638667698815
>1k	0.0010680684777039625	0.2737364971119591
>5k	0.0	0.0
>10k+	5.340342388519812E-4	0.9939219070836152
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10597	0.5051241717908385	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10172	0.48486581819915153	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2334	0.11125411125411126	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043519710186376855	0.0
2	0.0	0.0	0.0	0.20286953620286954	0.0
3	0.0	0.0	0.0	0.30187330187330186	0.0
4	0.0	0.0	0.0	0.4687068020401354	0.0
5	0.0	0.0	0.0	0.7750607750607751	0.0
6	0.0	0.0	0.0	1.1080127746794413	0.0
7	0.0	0.0	0.0	1.3126936460269794	0.0
8	0.0	0.0	0.0	1.8480385147051814	0.0
9	0.0	0.0	0.0	2.1039134372467707	0.0
10	0.0	0.0	0.0	2.5225225225225225	0.0
11	0.0	0.0	0.0	2.9165355832022497	0.0
12	0.0	0.0	0.0	3.2505362505362507	0.0
13	0.0	0.0	0.0	3.3846227179560513	0.0
14	0.0	0.0	0.0	3.434863434863435	0.0
15	0.0	0.0	0.0	3.516945516945517	0.0
16	0.0	0.0	0.0	3.7024167024167025	0.0
17	0.0	0.0	0.0	3.8954668954668956	0.0
18	0.0	0.0	0.0	4.216073216073216	0.0
19	0.0	0.0	0.0	4.327613327613328	0.0
20	0.0	0.0	0.0	4.461127794461127	0.0
21	0.0	0.0	0.0	4.620000953334286	0.0
22	0.0	0.0	0.0	4.770818437485104	0.0
23	0.0	0.0	0.0	4.942990609657277	0.0
24	0.0	0.0	0.0	5.0659707326374	0.0
25	0.0	0.0	0.0	5.157300157300157	0.0
26	0.0	0.0	0.0	5.256208589541923	0.0
27	0.0	0.0	0.0	5.353544020210687	0.0
28	0.0	0.0	0.0	5.447733447733448	0.0
29	0.0	0.0	0.0	5.5490252156918825	0.0
30	0.0	0.0	0.0	5.693550693550693	0.0
31	0.0	0.0	0.0	5.823108823108823	0.0
32	0.0	0.0	0.0	5.942132608799276	0.0
33	0.0	0.0	0.0	6.045283378616712	0.0
34	0.0	0.0	0.0	6.168311168311169	0.0
35	0.0	0.0	0.0	6.332808999475666	0.0
36	4.7666714333381E-5	0.0	0.0	6.461318461318461	0.0
37	4.7666714333381E-5	0.0	0.0	6.588016588016588	0.0
38	4.7666714333381E-5	0.0	0.0	6.709614376281043	0.0
39	4.7666714333381E-5	0.0	0.0	6.845702845702846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	25	3.8919097E-5	45.000004	14
GTACCGG	45	3.8562575E-10	45.000004	24
TCCGCAT	30	2.1663618E-6	45.000004	43
CACGGAT	20	7.034618E-4	45.000004	23
TCCGATT	30	2.1663618E-6	45.000004	10
CGTTGCA	30	2.1663618E-6	45.000004	24
TTACTCG	25	3.8919097E-5	45.000004	44
CGTCTAC	20	7.034618E-4	45.000004	16
ATTCGAC	20	7.034618E-4	45.000004	14
CAATTCG	20	7.034618E-4	45.000004	1
TGACGTT	30	2.1663618E-6	45.000004	21
TAACGAA	25	3.8919097E-5	45.000004	19
CGACAAA	20	7.034618E-4	45.000004	31
TCTAGCG	90	0.0	45.000004	1
CGTTACA	20	7.034618E-4	45.000004	45
TAGCACG	110	0.0	45.000004	1
ACTAGTC	40	6.8193913E-9	45.000004	10
TCTACGT	25	3.8919097E-5	45.000004	35
CGTACGT	25	3.8919097E-5	45.000004	42
TACTCGA	25	3.8919097E-5	45.000004	45
>>END_MODULE