##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1915622
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208749429689156	31.0	31.0	33.0	30.0	34.0
2	31.44224643483944	31.0	31.0	34.0	30.0	34.0
3	31.536449257734564	31.0	31.0	34.0	30.0	34.0
4	35.30670455862378	37.0	35.0	37.0	33.0	37.0
5	35.0220022530541	37.0	35.0	37.0	32.0	37.0
6	35.16566159712094	37.0	35.0	37.0	32.0	37.0
7	35.70741357115339	37.0	35.0	37.0	35.0	37.0
8	35.77483658049448	37.0	35.0	37.0	35.0	37.0
9	37.51934410859762	39.0	37.0	39.0	35.0	39.0
10	36.786370693174334	39.0	37.0	39.0	32.0	39.0
11	36.48236238673392	39.0	35.0	39.0	32.0	39.0
12	35.57642374121826	37.0	35.0	39.0	31.0	39.0
13	35.07569917238369	37.0	34.0	39.0	30.0	39.0
14	36.03426511075776	38.0	35.0	40.0	30.0	41.0
15	36.32283300150029	38.0	35.0	40.0	30.0	41.0
16	36.48088558181102	38.0	35.0	40.0	31.0	41.0
17	36.43085170247575	38.0	35.0	40.0	31.0	41.0
18	36.40840050907747	38.0	35.0	40.0	31.0	41.0
19	36.3627824278485	37.0	35.0	40.0	31.0	41.0
20	36.27001778012573	37.0	35.0	40.0	31.0	41.0
21	36.12395242902827	37.0	34.0	40.0	30.0	41.0
22	35.9989731794686	37.0	34.0	40.0	30.0	41.0
23	35.994183090400924	37.0	34.0	40.0	30.0	41.0
24	35.86059306063513	37.0	34.0	40.0	30.0	41.0
25	35.744717381612865	37.0	34.0	40.0	30.0	41.0
26	35.694091005428	37.0	34.0	40.0	30.0	41.0
27	35.62832333310016	37.0	34.0	40.0	30.0	41.0
28	35.63142206552232	37.0	34.0	40.0	29.0	41.0
29	35.66924267940126	37.0	34.0	40.0	30.0	41.0
30	35.58667367570429	36.0	34.0	40.0	29.0	41.0
31	35.48428395581174	36.0	34.0	40.0	29.0	41.0
32	35.325813234552534	36.0	34.0	40.0	29.0	41.0
33	35.162850499733246	36.0	34.0	40.0	28.0	41.0
34	35.04130929797215	36.0	34.0	40.0	27.0	41.0
35	34.90794530444941	36.0	34.0	40.0	27.0	41.0
36	34.72245307268344	36.0	33.0	40.0	25.0	41.0
37	34.6528547907677	36.0	33.0	40.0	25.0	41.0
38	34.665854745873666	36.0	33.0	40.0	26.0	41.0
39	34.612562394877486	36.0	33.0	40.0	26.0	41.0
40	34.45374400586337	36.0	33.0	40.0	24.0	41.0
41	34.40028878348651	36.0	33.0	40.0	24.0	41.0
42	34.360053288174804	35.0	33.0	40.0	24.0	41.0
43	34.31306854901437	35.0	33.0	40.0	25.0	41.0
44	34.10953778981448	35.0	33.0	40.0	24.0	41.0
45	34.08547510939006	35.0	33.0	40.0	24.0	41.0
46	34.01086226823455	35.0	33.0	40.0	24.0	41.0
47	33.98753877330705	35.0	33.0	39.0	24.0	41.0
48	33.90515665407894	35.0	33.0	39.0	24.0	41.0
49	33.83503895862545	35.0	33.0	39.0	24.0	41.0
50	33.762197343734826	35.0	33.0	39.0	24.0	41.0
51	33.680883806930595	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	1.0
13	6.0
14	21.0
15	44.0
16	175.0
17	547.0
18	1167.0
19	2169.0
20	3783.0
21	5892.0
22	8962.0
23	12670.0
24	17683.0
25	24015.0
26	31084.0
27	37170.0
28	42144.0
29	49161.0
30	59401.0
31	72033.0
32	89661.0
33	114883.0
34	192417.0
35	268630.0
36	137024.0
37	169036.0
38	233715.0
39	341946.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.060802183311736	24.397245385571892	29.191876059055495	13.350076372060876
2	29.146460000981406	26.720094047781867	30.993588505456714	13.139857445780013
3	29.476796570513393	25.447400374395368	31.491024847281977	13.584778207809265
4	26.86135364910196	28.499724893533273	30.168321307648377	14.470600149716384
5	23.54415432689748	33.06445634890391	29.00405194761806	14.38733737658056
6	21.75883342329541	41.57417277521348	25.950839988264907	10.716153813226201
7	86.6274766107301	5.727904565723301	5.851206553276168	1.7934122702704396
8	88.22559983128195	4.235177921322682	5.535643253209662	2.0035789941857005
9	84.08480378696841	6.169640983450806	6.8574071502624205	2.8881480793183623
10	50.991844946445596	24.704508509507615	13.123779117174475	11.179867426872315
11	46.76799493845863	21.406571860210416	18.526828361754042	13.29860483957691
12	42.361749865056886	22.038951317117885	21.972810919899647	13.626487897925582
13	24.089877856904966	38.16890806223775	21.511237603243227	16.22997647761406
14	16.548254300691887	41.26461274719125	25.284946612640695	16.90218633947616
15	15.82013570526962	24.58099771249234	44.068871624986556	15.529994957251484
16	17.289788904073976	19.52201425959819	43.34999284827592	19.838203988051923
17	17.386572089900827	20.02399220723086	28.777806895097257	33.81162880777106
18	22.068497856048843	25.314806365765268	31.416062250276934	21.200633527908952
19	30.689614130553938	25.7570648071488	23.142718135415024	20.410602926882234
20	32.60810326880773	24.191724672195246	23.513668145385676	19.68650391361135
21	23.6425035836924	28.355437555008244	25.53155058774643	22.470508273552923
22	22.697327552095352	27.488512869449195	23.602151155081742	26.21200842337371
23	21.891584039022312	29.792359870579894	23.95540456311318	24.36065152728461
24	21.765463123726914	24.640926028203893	35.54667883329801	18.04693201477118
25	20.175379067477824	26.39288962018603	32.27103259411304	21.160698718223113
26	18.190227508349768	34.10129973449877	25.703713989503147	22.004758767648312
27	19.567169305844264	33.11477942934462	27.48543292987865	19.832618334932466
28	16.915341335607962	29.634343309901436	34.89435807273043	18.55595728176018
29	17.80278155084876	26.33739850555068	34.19197524355014	21.667844700050427
30	20.555464491428893	29.875883655543735	29.48942954298917	20.079222310038205
31	28.504423106437493	28.605695695706146	23.398822941060395	19.491058256795966
32	31.07230967278513	27.7433126159545	23.3792992563251	17.805078454935263
33	28.32291548123795	29.18644701303284	22.85022828094478	19.64040922478443
34	20.40047566795537	28.368488146408843	27.797498671449794	23.433537514185993
35	21.98340800011693	25.994376761177307	28.849115326510137	23.17309991219562
36	28.141668867866414	28.450706872232622	24.562152658509874	18.84547160139109
37	21.79010264029125	33.41744874510733	26.914913276210022	17.877535338391397
38	21.251374227274482	33.38127250574487	23.543371291413443	21.823981975567204
39	22.311708677390424	32.01628504997333	25.24866596854703	20.423340304089223
40	24.160298848102602	26.542031778712083	25.12264945798284	24.175019915202476
41	18.694397955337745	25.528314041079085	26.593190097002434	29.184097906580735
42	22.045058993893367	28.01831467794795	22.255852146195856	27.680774181962832
43	22.062651191101377	27.6192797952832	24.611744905832154	25.706324107783267
44	21.070858447021383	30.611780403440765	26.066938049364648	22.250423100173208
45	18.80026435277941	35.624930179336005	22.479226068608522	23.09557939927606
46	21.344346640412358	34.089240988044615	24.44762066837821	20.11879170316482
47	22.14674920208684	28.763921065847022	26.5466777892507	22.54265194281544
48	22.77688395727341	24.555053136787947	29.611322066670777	23.05674083926787
49	21.210082156082986	25.723185471872846	29.77309719767261	23.293635174371563
50	19.71913039211285	32.3158222237999	26.131355768518006	21.83369161556925
51	18.62340273811848	32.76424054432451	24.80400621834579	23.808350499211222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1116.0
1	1802.0
2	2488.0
3	6264.0
4	10040.0
5	7820.5
6	5601.0
7	5948.5
8	6296.0
9	6771.5
10	7247.0
11	7309.5
12	7372.0
13	7131.5
14	6891.0
15	6751.0
16	6611.0
17	6055.0
18	5499.0
19	5525.0
20	5551.0
21	5866.0
22	6181.0
23	7421.5
24	8662.0
25	9789.5
26	12436.5
27	13956.0
28	19971.5
29	25987.0
30	28157.0
31	30327.0
32	35664.0
33	41001.0
34	47069.5
35	53138.0
36	53624.0
37	54110.0
38	65663.0
39	77216.0
40	104304.0
41	131392.0
42	154257.0
43	177122.0
44	179547.5
45	181973.0
46	170239.0
47	158505.0
48	150392.5
49	142280.0
50	134610.0
51	126940.0
52	120682.0
53	114424.0
54	106238.0
55	98052.0
56	92550.5
57	87049.0
58	86613.5
59	86178.0
60	79962.0
61	73746.0
62	65300.5
63	56855.0
64	45732.0
65	34609.0
66	28138.0
67	21667.0
68	18028.0
69	14389.0
70	12040.5
71	9692.0
72	8232.5
73	6773.0
74	5614.0
75	3062.5
76	1670.0
77	1275.0
78	880.0
79	693.5
80	507.0
81	336.0
82	165.0
83	106.5
84	48.0
85	36.0
86	24.0
87	17.5
88	11.0
89	7.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1915622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.58681930480381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79192977228823	14.208730701916325
2	7.3464689494988455	2.5840204388637615
3	2.407953130655771	1.2704470980983908
4	1.15479898792418	0.8123696453597149
5	0.6772093354218118	0.595497910679484
6	0.45638278197171867	0.4815792912216174
7	0.33469237446496625	0.4120322018687766
8	0.25095279301349177	0.35307691398112895
9	0.23990558092399833	0.3797258491731912
>10	3.1654453608596054	13.922505261125123
>50	1.5655956388762267	19.950807410460133
>100	1.6008343078351635	43.292001385787486
>500	0.005421452030291096	0.6220803143481883
>1k	0.0018071506767636989	0.5722354935203916
>5k	6.023835589212329E-4	0.5428900835962689
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5164	0.26957301597079175	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5085	0.2654490290882021	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2906	0.15170007444057335	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2157	0.11260050260437603	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2079	0.10852871808738884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038055524524149335	0.0
2	0.0	0.0	0.0	0.12366740411208474	0.0
3	0.0	0.0	0.0	0.17780125724177318	0.0
4	0.0	0.0	0.0	0.2794392630696453	0.0
5	0.0	0.0	0.0	0.4620431379468392	0.0
6	0.0	0.0	0.0	0.645273441211262	0.0
7	0.0	0.0	0.0	0.7491561487600372	0.0
8	0.0	0.0	0.0	0.9876165548317988	0.0
9	0.0	0.0	0.0	1.0657634961385911	0.0
10	0.0	0.0	0.0	1.21715035638555	0.0
11	0.0	0.0	0.0	1.398449172122684	0.0
12	0.0	0.0	0.0	1.5460252596806676	0.0
13	0.0	0.0	0.0	1.6051183375425842	0.0
14	0.0	0.0	0.0	1.6269911287299896	0.0
15	0.0	0.0	0.0	1.668596414115102	0.0
16	0.0	0.0	0.0	1.7677809087596614	0.0
17	0.0	0.0	0.0	1.8770404599654837	0.0
18	0.0	0.0	0.0	2.019970536984854	0.0
19	0.0	0.0	0.0	2.0921142062473703	0.0
20	5.220236560239964E-5	0.0	0.0	2.1621697808857907	0.0
21	5.220236560239964E-5	0.0	0.0	2.262137311014386	0.0
22	5.220236560239964E-5	0.0	0.0	2.37186668351063	0.0
23	5.220236560239964E-5	0.0	0.0	2.499605872139702	0.0
24	5.220236560239964E-5	0.0	0.0	2.597485307644201	0.0
25	5.220236560239964E-5	0.0	0.0	2.676624093897439	0.0
26	5.220236560239964E-5	0.0	0.0	2.7585818078932065	0.0
27	5.220236560239964E-5	0.0	0.0	2.8347450593071075	0.0
28	5.220236560239964E-5	0.0	0.0	2.916493963840465	0.0
29	1.0440473120479928E-4	0.0	0.0	3.0084745320318937	0.0
30	1.0440473120479928E-4	0.0	0.0	3.1166378335600657	0.0
31	1.566070968071989E-4	0.0	0.0	3.2263672060563096	0.0
32	1.566070968071989E-4	0.0	0.0	3.3189742026349665	0.0
33	1.566070968071989E-4	0.0	0.0	3.4201945895380197	0.0
34	1.566070968071989E-4	0.0	0.0	3.531959854292757	0.0
35	1.566070968071989E-4	0.0	0.0	3.663614220342009	0.0
36	1.566070968071989E-4	0.0	0.0	3.7855589463892145	0.0
37	1.566070968071989E-4	0.0	0.0	3.901604805123349	0.0
38	1.566070968071989E-4	0.0	0.0	4.01843369934152	0.0
39	1.566070968071989E-4	0.0	0.0	4.141526877431978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	30	2.1662636E-6	45.000004	34
TCCGCGA	40	6.8193913E-9	45.000004	22
GTCGATC	30	2.1662636E-6	45.000004	36
ACGTTAC	50	2.1827873E-11	45.000004	1
TAGCGAT	20	7.034464E-4	45.000004	25
CTACGTC	25	3.8917824E-5	45.000004	1
CGCTATA	20	7.034464E-4	45.000004	27
ACGTGCC	25	3.8917824E-5	45.000004	45
ATGCGAT	40	6.8193913E-9	45.000004	12
TACGTCG	25	3.8917824E-5	45.000004	2
TACGTAG	65	0.0	45.000004	1
CGGTCTA	340	0.0	43.67647	31
GCGATAT	270	0.0	43.333332	9
TGCGTAG	125	0.0	43.2	1
ACGGGTA	140	0.0	41.785713	5
GTATGCG	65	0.0	41.538464	1
TAATGCG	65	0.0	41.538464	1
GCGTAAG	185	0.0	41.351353	1
ACGTTAG	120	0.0	41.250004	1
TGCGCGT	55	6.184564E-11	40.909092	37
>>END_MODULE