##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1753022
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203211939154215	31.0	31.0	33.0	30.0	34.0
2	31.43557924544016	31.0	31.0	34.0	30.0	34.0
3	31.5228650866903	31.0	31.0	34.0	30.0	34.0
4	35.28708196474431	37.0	35.0	37.0	33.0	37.0
5	35.01068611802933	37.0	35.0	37.0	32.0	37.0
6	35.151625592833405	37.0	35.0	37.0	32.0	37.0
7	35.69188521307776	37.0	35.0	37.0	35.0	37.0
8	35.769618977970616	37.0	35.0	37.0	35.0	37.0
9	37.48824144819631	39.0	37.0	39.0	35.0	39.0
10	36.80040182039929	39.0	37.0	39.0	32.0	39.0
11	36.479476583864894	39.0	35.0	39.0	32.0	39.0
12	35.46563591329715	37.0	35.0	39.0	30.0	39.0
13	34.92124342991702	37.0	34.0	39.0	28.0	39.0
14	35.837517156088175	38.0	34.0	40.0	29.0	41.0
15	36.15989131910495	38.0	35.0	40.0	30.0	41.0
16	36.36299943754271	38.0	35.0	40.0	31.0	41.0
17	36.328517269035984	38.0	35.0	40.0	31.0	41.0
18	36.31816714222639	37.0	35.0	40.0	31.0	41.0
19	36.247817197958724	37.0	35.0	40.0	31.0	41.0
20	36.155670607670636	37.0	34.0	40.0	31.0	41.0
21	35.98315480353356	37.0	34.0	40.0	30.0	41.0
22	35.82967641022189	37.0	34.0	40.0	30.0	41.0
23	35.83307625346402	36.0	34.0	40.0	30.0	41.0
24	35.741668387504546	36.0	34.0	40.0	30.0	41.0
25	35.61227297774928	36.0	34.0	40.0	30.0	41.0
26	35.550384992316125	36.0	34.0	40.0	30.0	41.0
27	35.46555376943358	36.0	34.0	40.0	29.0	41.0
28	35.48996019445278	36.0	34.0	40.0	29.0	41.0
29	35.53079311041162	36.0	34.0	40.0	30.0	41.0
30	35.467358082214595	36.0	34.0	40.0	29.0	41.0
31	35.36875350109696	36.0	34.0	40.0	29.0	41.0
32	35.17318208214158	36.0	34.0	40.0	29.0	41.0
33	35.02355988686965	36.0	34.0	40.0	27.0	41.0
34	34.909092983430895	36.0	34.0	40.0	27.0	41.0
35	34.758776558423115	36.0	34.0	40.0	27.0	41.0
36	34.57854835820657	36.0	33.0	40.0	25.0	41.0
37	34.51962496762733	35.0	33.0	40.0	25.0	41.0
38	34.529655075635105	35.0	33.0	40.0	26.0	41.0
39	34.492732549848206	35.0	33.0	40.0	25.0	41.0
40	34.30276802002485	35.0	33.0	40.0	24.0	41.0
41	34.25616221587636	35.0	33.0	40.0	24.0	41.0
42	34.21541315511157	35.0	33.0	40.0	24.0	41.0
43	34.16904921900581	35.0	33.0	40.0	24.0	41.0
44	33.943972751055036	35.0	33.0	40.0	23.0	41.0
45	33.94659336848026	35.0	33.0	40.0	23.0	41.0
46	33.900866047317145	35.0	33.0	39.0	24.0	41.0
47	33.85846327085456	35.0	33.0	39.0	23.0	41.0
48	33.80315192849833	35.0	33.0	39.0	23.0	41.0
49	33.7351328163594	35.0	33.0	39.0	24.0	41.0
50	33.68191386075018	35.0	33.0	39.0	24.0	41.0
51	33.57996305807914	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	5.0
13	7.0
14	16.0
15	63.0
16	183.0
17	496.0
18	1168.0
19	2109.0
20	3765.0
21	5831.0
22	8619.0
23	12234.0
24	16962.0
25	22951.0
26	29396.0
27	34796.0
28	38611.0
29	45396.0
30	54321.0
31	66817.0
32	83473.0
33	108355.0
34	183905.0
35	260377.0
36	120999.0
37	146898.0
38	205147.0
39	299988.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.87304894062938	24.75787525769785	27.76816263572277	13.600913165949999
2	29.46340662011087	26.896182706206766	30.336413347921475	13.303997325760886
3	29.524957473437297	25.731337085330363	30.99784258269434	13.745862858537999
4	27.259384080747417	28.520919874365525	29.787589659456643	14.432106385430416
5	23.613964913161386	33.133240769368555	29.244299272912716	14.008495044557343
6	21.308004120883822	41.78099305085732	26.28734836185741	10.62365446640145
7	86.18916362715356	5.805175291582194	6.122056654166348	1.8836044270978916
8	88.25342750975173	4.501997122683001	5.355323549847064	1.8892518177182034
9	83.61098719810704	6.5343732137987995	7.0963741470443615	2.7582654410497986
10	53.3894611704816	22.912433500549337	13.23811110185725	10.45999422711181
11	48.39722490647579	21.028772029101745	17.601490454768964	12.9725126096535
12	43.814738206365924	21.62728134615538	21.513249691104846	13.044730756373852
13	23.49069207345943	41.01534378918234	20.994602463631377	14.499361673726854
14	16.08941587726794	43.87149733431754	24.95958407823747	15.079502710177053
15	14.543000601247446	24.21156152061982	46.318814025151994	14.92662385298074
16	16.526831950768443	18.43234140815118	45.50781450546542	19.533012135614953
17	16.486672728579563	20.427068228464904	27.465827582312148	35.620431460643395
18	22.017293565055088	24.165640819111225	32.45344325399225	21.363622361841436
19	30.388780060946186	24.998659457782047	23.624346984806806	20.98821349646496
20	33.32753382444715	23.093948621295112	23.075009897194672	20.50350765706306
21	23.616018509750592	29.18263433088689	25.214629365746692	21.986717793615824
22	23.50945966451077	26.583351492451325	23.159378490401146	26.74781035263676
23	20.879658098985637	30.85665781718655	23.280540689164198	24.983143394663614
24	21.182620640242963	24.577443979596378	36.64489093690781	17.595044443252853
25	18.392068097262896	26.250326578902033	33.80459572098924	21.55300960284583
26	18.191842429815487	34.569503406118116	25.875031802224957	21.363622361841436
27	18.589384502875607	34.73356295585566	27.581513523503983	19.09553901776475
28	16.39488836991207	29.10231588650913	36.509068340271824	17.993727403306973
29	16.730138013099666	24.66169848410345	36.716823861879654	21.891339640917227
30	18.970783024970594	31.4118704728178	30.798871890940333	18.81847461127128
31	29.270197407676573	27.698967839536525	22.969991249396756	20.060843503390146
32	30.787520065350005	27.232002792891365	24.148869780299393	17.83160736145924
33	29.015209164517046	28.840596410084984	22.78767750775518	19.35651691764279
34	20.272877351225485	27.896968777345638	27.23240210333926	24.59775176808962
35	21.226887055610256	26.561560550865877	28.99798177090761	23.21357062261626
36	29.386853102813316	26.362875080860366	25.043268139247537	19.20700367707878
37	20.919703232475122	33.47522164582076	26.807535786772785	18.797539334931336
38	21.015081385173715	34.06101007289127	23.089270984619702	21.834637557315308
39	20.824553257175324	33.238259417166475	24.987935120038426	20.949252205619782
40	24.377788755645966	27.181290366007953	24.654396807341836	23.78652407100424
41	18.1499148327859	26.145022709355615	26.02625637327997	29.678806084578518
42	22.081069148019818	27.52646572604337	22.50268393665339	27.88978118928342
43	22.77986243184626	27.59959658235892	24.38959693603389	25.23094404976093
44	20.593010241742547	30.77457099796808	26.666351021264994	21.966067739024382
45	17.62408001725021	37.98286615912407	21.47194958192196	22.921104241703755
46	21.94062595905813	34.376750548481425	24.53648613651169	19.146137355948756
47	22.679236198975257	28.609224527701304	25.843486276840792	22.868052996482646
48	23.443573440607135	24.663010504146555	29.173393146235473	22.72002290901084
49	20.935960872139653	25.17338630091351	31.19088066207954	22.699772164867298
50	19.4092829411154	34.09221333217724	25.805323606891413	20.693180119815953
51	19.05640659387047	33.79883424166953	23.625601960500212	23.51915720395979
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1124.0
1	1574.0
2	2024.0
3	5007.0
4	7990.0
5	6287.0
6	4584.0
7	4806.0
8	5028.0
9	5522.0
10	6016.0
11	6412.0
12	6808.0
13	6539.0
14	6270.0
15	6089.5
16	5909.0
17	5772.0
18	5635.0
19	6641.0
20	7647.0
21	6677.0
22	5707.0
23	5694.5
24	5682.0
25	7236.0
26	11292.5
27	13795.0
28	16895.0
29	19995.0
30	23499.5
31	27004.0
32	31037.5
33	35071.0
34	40081.5
35	45092.0
36	47351.5
37	49611.0
38	62643.0
39	75675.0
40	98754.0
41	121833.0
42	145640.5
43	169448.0
44	172949.5
45	176451.0
46	166967.0
47	157483.0
48	144840.5
49	132198.0
50	131516.0
51	130834.0
52	119359.0
53	107884.0
54	99354.5
55	90825.0
56	82527.5
57	74230.0
58	70634.5
59	67039.0
60	63244.0
61	59449.0
62	48683.5
63	37918.0
64	33589.5
65	29261.0
66	23626.5
67	17992.0
68	15186.0
69	12380.0
70	10929.0
71	9478.0
72	8098.0
73	6718.0
74	4877.5
75	2234.0
76	1431.0
77	1098.5
78	766.0
79	621.0
80	476.0
81	361.5
82	247.0
83	197.0
84	147.0
85	87.5
86	28.0
87	17.0
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1753022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.010181279301392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3586441418579	13.839252854911752
2	7.171964621799488	2.439928366910911
3	2.3271473948560213	1.187555971504647
4	1.1169468824503979	0.7599787579932724
5	0.7001730507226522	0.5955035259836902
6	0.4257835989064006	0.43455937218907364
7	0.3387802806015486	0.4033899790819454
8	0.26349550689954176	0.3585685070914093
9	0.20637162319194843	0.315937684925885
>10	2.9244601862718316	12.187695541690196
>50	1.35292224000175	16.867714572493856
>100	1.8048036678222328	48.96320282021364
>500	0.006465171509921245	0.6781906865159549
>1k	0.0017013609236634854	0.579735249308168
>5k	3.402721847326971E-4	0.38878610918559947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6717	0.3831668969356916	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4173	0.23804607129859182	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1950	0.1112364819152298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026012223463253742	0.0
2	0.0	0.0	0.0	0.07432878765925356	0.0
3	0.0	0.0	0.0	0.10730042178592168	0.0
4	0.0	0.0	0.0	0.17489797618056133	0.0
5	0.0	0.0	0.0	0.2936643122562067	0.0
6	0.0	0.0	0.0	0.418192127651564	0.0
7	0.0	0.0	0.0	0.4965710641395259	0.0
8	0.0	0.0	0.0	0.7126550608035723	0.0
9	0.0	0.0	0.0	0.7909769529418341	0.0
10	0.0	0.0	0.0	0.9337019158915291	0.0
11	0.0	0.0	0.0	1.0777959432340267	0.0
12	0.0	0.0	0.0	1.2193229748400192	0.0
13	0.0	0.0	0.0	1.2729446635581299	0.0
14	0.0	0.0	0.0	1.2910277224130673	0.0
15	0.0	0.0	0.0	1.3278213279696434	0.0
16	0.0	0.0	0.0	1.4068847966540066	0.0
17	0.0	0.0	0.0	1.4909111237622803	0.0
18	0.0	0.0	0.0	1.6077379519481216	0.0
19	0.0	0.0	0.0	1.6634132372554367	0.0
20	0.0	0.0	0.0	1.7274169976189688	0.0
21	0.0	0.0	0.0	1.807393175898534	0.0
22	0.0	0.0	0.0	1.8945569422403141	0.0
23	0.0	0.0	0.0	1.9930725341724176	0.0
24	0.0	0.0	0.0	2.0715085150100796	0.0
25	0.0	0.0	0.0	2.132488924839506	0.0
26	0.0	0.0	0.0	2.193241157270131	0.0
27	0.0	0.0	0.0	2.255077232345059	0.0
28	0.0	0.0	0.0	2.319594391855892	0.0
29	0.0	0.0	0.0	2.391926627275642	0.0
30	0.0	0.0	0.0	2.487304779974239	0.0
31	0.0	0.0	0.0	2.5699620426897094	0.0
32	0.0	0.0	0.0	2.65256226105548	0.0
33	0.0	0.0	0.0	2.7355047455194517	0.0
34	0.0	0.0	0.0	2.82198397966483	0.0
35	0.0	0.0	0.0	2.9402369165931743	0.0
36	0.0	0.0	0.0	3.033903738800768	0.0
37	0.0	0.0	0.0	3.1328186411807724	0.0
38	0.0	0.0	0.0	3.217358367436347	0.0
39	0.0	0.0	0.0	3.3156457819696503	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	30	2.1661563E-6	45.000004	12
CGACAGT	25	3.8916463E-5	45.000004	24
CTACGTT	25	3.8916463E-5	45.000004	28
ACCGTGC	30	2.1661563E-6	45.000004	25
CCAACGA	30	2.1661563E-6	45.000004	45
TCGAACA	50	2.1827873E-11	45.000004	24
ACGCCCG	20	7.0343027E-4	45.0	32
GACGTAT	20	7.0343027E-4	45.0	21
CACGCTA	20	7.0343027E-4	45.0	40
CGACACG	20	7.0343027E-4	45.0	11
AATGCGC	20	7.0343027E-4	45.0	42
ATATCGT	20	7.0343027E-4	45.0	12
TGTTACG	45	3.8562575E-10	45.0	1
CGTCGAA	20	7.0343027E-4	45.0	34
TCGCGAT	20	7.0343027E-4	45.0	1
CGTTTTT	4475	0.0	42.988827	1
GCGATAT	210	0.0	42.857143	9
GGCGATA	600	0.0	41.625	8
CCCGAAC	65	0.0	41.53846	41
CGACGGT	225	0.0	41.0	28
>>END_MODULE