FastQCFastQC Report
Sat 18 Jun 2016
SRR3549173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855460
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC82970.4471667403231544No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39910.2150949090791502No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23620.1272999687409052No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23050.12422795425393164No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20430.11010746661205308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.034407E-445.00000421
CGCGATA207.034407E-445.00000411
GTAATCG351.2124656E-745.0000041
CGTTAGC207.034407E-445.00000440
TAGCACG351.2124656E-745.0000041
GCCGAAT207.034407E-445.00000445
CGTAGCT406.8193913E-945.00000419
TATTACG650.045.0000041
GTCCGAT207.034407E-445.00000430
TCGTATA207.034407E-445.00000429
GCGATAC1250.045.0000049
GGTACGA253.891736E-545.06
CTATACG453.8562575E-1045.01
TGTTGCG1000.045.01
GATTCGC253.891736E-545.041
GTACGAC253.891736E-545.019
CAAGTCG302.1662272E-644.9999961
GACCGTT302.1662272E-644.99999632
ATCCGTT302.1662272E-644.99999611
TCGTAGA302.1662272E-644.99999624