##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549173_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1855460 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.277609325989243 31.0 31.0 33.0 30.0 34.0 2 31.521375831330236 31.0 31.0 34.0 30.0 34.0 3 31.604343936274564 31.0 31.0 34.0 30.0 34.0 4 35.37458581699417 37.0 35.0 37.0 33.0 37.0 5 35.0937778232891 37.0 35.0 37.0 32.0 37.0 6 35.2350209651515 37.0 35.0 37.0 32.0 37.0 7 35.74319737423604 37.0 35.0 37.0 35.0 37.0 8 35.80908507863279 37.0 35.0 37.0 35.0 37.0 9 37.55159960333287 39.0 37.0 39.0 35.0 39.0 10 36.89437120714001 39.0 37.0 39.0 32.0 39.0 11 36.55591928686148 39.0 35.0 39.0 32.0 39.0 12 35.45791447942828 37.0 35.0 39.0 31.0 39.0 13 34.87447317646298 37.0 34.0 39.0 28.0 39.0 14 35.79077587229043 38.0 34.0 40.0 29.0 41.0 15 36.15512056309487 38.0 35.0 40.0 30.0 41.0 16 36.372883813178404 37.0 35.0 40.0 31.0 41.0 17 36.34158968665452 37.0 35.0 40.0 31.0 41.0 18 36.33518157222468 37.0 35.0 40.0 31.0 41.0 19 36.25351125866362 37.0 35.0 40.0 31.0 41.0 20 36.15789615513134 37.0 35.0 40.0 31.0 41.0 21 35.981473057894 36.0 34.0 40.0 30.0 41.0 22 35.86841591842454 36.0 34.0 40.0 30.0 41.0 23 35.85941491597771 36.0 34.0 40.0 30.0 41.0 24 35.741799338169514 36.0 34.0 40.0 30.0 41.0 25 35.596204714733815 36.0 34.0 40.0 30.0 41.0 26 35.551325816778586 36.0 34.0 40.0 30.0 41.0 27 35.478169833895635 36.0 34.0 40.0 30.0 41.0 28 35.49595248617594 36.0 34.0 40.0 30.0 41.0 29 35.56349045519709 36.0 34.0 40.0 30.0 41.0 30 35.496102853200824 36.0 34.0 40.0 30.0 41.0 31 35.36495370420273 36.0 34.0 40.0 29.0 41.0 32 35.20348161641857 35.0 34.0 40.0 29.0 41.0 33 35.041844071012044 35.0 34.0 40.0 29.0 41.0 34 34.92545190949953 35.0 34.0 40.0 27.0 41.0 35 34.77444784581721 35.0 34.0 40.0 27.0 41.0 36 34.569134877604476 35.0 33.0 40.0 25.0 41.0 37 34.47376715208089 35.0 33.0 40.0 25.0 41.0 38 34.50921981611029 35.0 33.0 40.0 26.0 41.0 39 34.46173347849051 35.0 33.0 40.0 26.0 41.0 40 34.28365903872894 35.0 33.0 40.0 24.0 41.0 41 34.266062863117504 35.0 33.0 40.0 24.0 41.0 42 34.2161760425986 35.0 33.0 40.0 25.0 41.0 43 34.169419443156954 35.0 33.0 40.0 25.0 41.0 44 33.933451004063684 35.0 33.0 39.0 24.0 41.0 45 33.93113998684962 35.0 33.0 39.0 24.0 41.0 46 33.88220495187177 35.0 33.0 39.0 24.0 41.0 47 33.85324771215763 35.0 33.0 39.0 24.0 41.0 48 33.795964343073955 35.0 33.0 39.0 24.0 41.0 49 33.74419389261962 35.0 33.0 39.0 24.0 41.0 50 33.67264236361873 35.0 33.0 39.0 24.0 41.0 51 33.57691623640499 35.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 4.0 13 4.0 14 17.0 15 55.0 16 205.0 17 483.0 18 1075.0 19 2003.0 20 3544.0 21 5707.0 22 8501.0 23 12437.0 24 17129.0 25 23079.0 26 30086.0 27 35057.0 28 40632.0 29 47079.0 30 56935.0 31 70394.0 32 88300.0 33 115486.0 34 201958.0 35 295510.0 36 126301.0 37 151327.0 38 208189.0 39 313762.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.524279693445294 25.513349789270585 29.182844146465026 12.779526370819097 2 28.6981125974152 27.688821100966877 30.681394371207137 12.931671930410788 3 28.756319187694697 25.666465458700266 31.69515915190842 13.882056201696614 4 27.069998814310196 28.8720317333707 29.78506677589385 14.272902676425254 5 23.077188406109535 34.22612182423765 28.968611557241868 13.728078212410939 6 21.840998997553168 41.200672609487675 26.327918683237577 10.63040970972158 7 87.09026333092602 5.59532407058088 5.545686783870307 1.7687258146227889 8 89.07155099005098 4.444719907731775 4.945889429036465 1.5378396731807746 9 84.63663997068113 6.472195574143338 6.336272406842508 2.554892048333028 10 54.86704105720415 23.851875006736876 11.035969516993092 10.245114419065892 11 50.76196738275145 19.8020975930497 17.574725405020857 11.861209619177995 12 45.51308031431559 22.030547680898536 20.60815107843877 11.848220926347105 13 21.953154473823204 44.374871999396376 20.021504101408816 13.650469425371606 14 14.818050510385564 45.42070429974238 25.034816164185703 14.726429025686352 15 13.975294536125812 23.449495003934334 48.54251775839953 14.03269270154032 16 14.613195649596328 17.657831481142143 47.358660386103715 20.370312483157814 17 15.512972524333588 19.686654522328695 27.17229150722732 37.6280814461104 18 21.567158548284524 24.48228471645845 32.870608905608314 21.079947829648713 19 31.009022021493323 24.33827730050769 23.514600153061775 21.13810052493721 20 33.42869153740851 23.122567988531145 22.93840880428573 20.51033166977461 21 22.273021245405452 29.811098056546626 25.76520108221142 22.150679615836506 22 24.01064965022151 26.473111788990334 22.318831987755058 27.1974065730331 23 20.31749539197827 31.452416112446507 23.10241126189732 25.127677233677904 24 20.012018582992898 24.710206633395494 38.15366539833788 17.124109385273734 25 17.4534616752719 26.642934905629872 34.87102928653811 21.032574132560118 26 16.64266543067487 35.82092850290494 26.10215256594052 21.434253500479667 27 18.556422666077417 36.027400213424166 26.295743373610858 19.120433746887564 28 14.982268547961153 30.179524215019455 37.794024123397975 17.04418311362142 29 15.507259655287637 25.273786554277645 37.07943043773512 22.1395233526996 30 17.880848953898223 32.92218641199487 29.907947355372794 19.289017278734114 31 29.68250460802173 28.323811884923416 22.66861047934205 19.325073027712804 32 31.131633126017267 27.268925226089486 24.34425964450864 17.255182003384604 33 28.926519569271232 29.318821208756855 22.64764532784323 19.10701389412868 34 19.513436021256183 28.753139383225722 27.455994739848876 24.277429855669215 35 21.356267448503335 25.546495208735298 29.664449786036883 23.432787556724477 36 30.88721934183437 26.146777618488137 24.59055975337652 18.37544328630097 37 21.138908949802204 33.929914953704206 27.918198182660902 17.01297791383269 38 19.957476852101365 34.27327994136225 23.458225992476258 22.311017214060126 39 20.858708891595615 33.541817123516545 26.44320006898559 19.156273915902254 40 24.141237213413387 27.794401388334965 23.82826900067908 24.23609239757257 41 17.453946730190896 25.450292649801128 26.24626777187328 30.849492848134695 42 21.914565660267534 27.041272784107445 22.405333448309314 28.638828107315707 43 23.136526791200026 27.07899927780712 24.21868431547972 25.565789615513136 44 20.17817684024447 30.963426859107713 26.952561628922204 21.90583467172561 45 16.809147057872444 39.26519569271232 21.405150205339915 22.520507044075323 46 21.531911224170823 35.12067088484796 24.607482780550374 18.739935110430835 47 21.90001401269766 28.571998318476282 26.351955849223373 23.176031819602684 48 23.36762851260604 24.22434328953467 30.453741929225096 21.954286268634192 49 20.723701939141776 24.735698964138273 31.3835383139491 23.15706078277085 50 19.31741993899087 34.05182542334516 26.332068597544545 20.29868604011943 51 19.518502150410143 34.212863656451766 22.685749086479902 23.58288510665819 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1235.0 1 1702.5 2 2170.0 3 4997.5 4 7825.0 5 6273.0 6 4721.0 7 5183.5 8 5646.0 9 6277.0 10 6908.0 11 6982.0 12 7056.0 13 7047.5 14 7039.0 15 6742.5 16 6446.0 17 6310.0 18 6174.0 19 6353.5 20 6533.0 21 6562.0 22 6591.0 23 6680.5 24 6770.0 25 8824.5 26 12257.0 27 13635.0 28 18083.0 29 22531.0 30 25744.0 31 28957.0 32 35918.0 33 42879.0 34 48081.5 35 53284.0 36 55141.0 37 56998.0 38 65734.5 39 74471.0 40 107702.5 41 140934.0 42 163033.5 43 185133.0 44 190887.5 45 196642.0 46 181851.5 47 167061.0 48 156291.5 49 145522.0 50 137082.0 51 128642.0 52 118108.5 53 107575.0 54 97053.0 55 86531.0 56 80160.5 57 73790.0 58 68902.0 59 64014.0 60 59521.5 61 55029.0 62 48668.5 63 42308.0 64 37287.0 65 32266.0 66 25617.0 67 18968.0 68 15529.5 69 12091.0 70 10189.5 71 8288.0 72 6785.0 73 5282.0 74 4209.5 75 2519.0 76 1901.0 77 1410.0 78 919.0 79 648.5 80 378.0 81 271.0 82 164.0 83 111.0 84 58.0 85 60.0 86 62.0 87 38.0 88 14.0 89 8.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1855460.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.034059645612427 #Duplication Level Percentage of deduplicated Percentage of total 1 81.73120995621684 13.10483095346053 2 7.379640590983692 2.366511947980301 3 2.332860508785001 1.1221567362825737 4 1.0834351541611844 0.694874555358949 5 0.6276041011237035 0.5031520795624218 6 0.42643989181571484 0.4102537596385009 7 0.28594147757693095 0.32093618946361413 8 0.23426829996571352 0.3005017515781179 9 0.19531111963232492 0.28184671274724377 >10 2.474897977066965 9.765697743515739 >50 1.2322310883586491 14.493269700716397 >100 1.9818553277373707 54.26313657207547 >500 0.011239255166752553 1.062971190724642 >1k 0.0027246679192127403 0.8567662476896433 >5k 3.4058348990159253E-4 0.45309385920587486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 8297 0.4471667403231544 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3991 0.2150949090791502 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2362 0.1272999687409052 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2305 0.12422795425393164 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2043 0.11010746661205308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.034331109266704754 0.0 2 0.0 0.0 0.0 0.11048473154904984 0.0 3 0.0 0.0 0.0 0.15467862416866976 0.0 4 0.0 0.0 0.0 0.23455100082998287 0.0 5 0.0 0.0 0.0 0.369072898364826 0.0 6 0.0 0.0 0.0 0.5008461513586927 0.0 7 0.0 0.0 0.0 0.5787244133530229 0.0 8 0.0 0.0 0.0 0.7526974442995268 0.0 9 0.0 0.0 0.0 0.8233537774999191 0.0 10 0.0 0.0 0.0 0.9529173358628049 0.0 11 0.0 0.0 0.0 1.092074202623608 0.0 12 0.0 0.0 0.0 1.215709311976545 0.0 13 0.0 0.0 0.0 1.2614661593351515 0.0 14 0.0 0.0 0.0 1.2807066711219859 0.0 15 0.0 0.0 0.0 1.3135826156317032 0.0 16 0.0 0.0 0.0 1.3849395837150895 0.0 17 0.0 0.0 0.0 1.4693391396203637 0.0 18 0.0 0.0 0.0 1.5970702682892652 0.0 19 0.0 0.0 0.0 1.650480204369806 0.0 20 0.0 0.0 0.0 1.7122977590462742 0.0 21 0.0 0.0 0.0 1.7885052763196188 0.0 22 0.0 0.0 0.0 1.8618563590699881 0.0 23 0.0 0.0 0.0 1.943237795479288 0.0 24 0.0 0.0 0.0 2.0072650447867377 0.0 25 0.0 0.0 0.0 2.0638547853362508 0.0 26 0.0 0.0 0.0 2.119097151110776 0.0 27 0.0 0.0 0.0 2.17089023746133 0.0 28 0.0 0.0 0.0 2.225108598406864 0.0 29 0.0 0.0 0.0 2.2919922822372887 0.0 30 0.0 0.0 0.0 2.38140407230552 0.0 31 0.0 0.0 0.0 2.4642945684628073 0.0 32 0.0 0.0 0.0 2.540124820799155 0.0 33 0.0 0.0 0.0 2.6087870393325643 0.0 34 0.0 0.0 0.0 2.6874198312008883 0.0 35 0.0 0.0 0.0 2.7900897890550054 0.0 36 5.3894990999536504E-5 0.0 0.0 2.8696387957703213 0.0 37 5.3894990999536504E-5 0.0 0.0 2.94999622735063 0.0 38 5.3894990999536504E-5 0.0 0.0 3.030084183975941 0.0 39 5.3894990999536504E-5 0.0 0.0 3.1116812003492393 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTATCG 20 7.034407E-4 45.000004 21 CGCGATA 20 7.034407E-4 45.000004 11 GTAATCG 35 1.2124656E-7 45.000004 1 CGTTAGC 20 7.034407E-4 45.000004 40 TAGCACG 35 1.2124656E-7 45.000004 1 GCCGAAT 20 7.034407E-4 45.000004 45 CGTAGCT 40 6.8193913E-9 45.000004 19 TATTACG 65 0.0 45.000004 1 GTCCGAT 20 7.034407E-4 45.000004 30 TCGTATA 20 7.034407E-4 45.000004 29 GCGATAC 125 0.0 45.000004 9 GGTACGA 25 3.891736E-5 45.0 6 CTATACG 45 3.8562575E-10 45.0 1 TGTTGCG 100 0.0 45.0 1 GATTCGC 25 3.891736E-5 45.0 41 GTACGAC 25 3.891736E-5 45.0 19 CAAGTCG 30 2.1662272E-6 44.999996 1 GACCGTT 30 2.1662272E-6 44.999996 32 ATCCGTT 30 2.1662272E-6 44.999996 11 TCGTAGA 30 2.1662272E-6 44.999996 24 >>END_MODULE