FastQCFastQC Report
Sat 18 Jun 2016
SRR3549172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912734
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC68680.35906717818578016No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63530.3321423679403409No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG54380.28430508371786145No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG44170.23092599389146637No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA21340.11156804866750943No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20200.10560799358405298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCA253.8917802E-545.0000041
TATTACG406.8193913E-945.01
TCGTACA351.212502E-745.034
TCGTAAT207.0344645E-445.045
TCGACGA351.212502E-745.02
GTACGAC207.0344645E-445.037
CAAATCG600.044.9999961
TATCGTG302.1662618E-644.9999961
GCGATAT1100.042.9545449
TACGTAG1450.041.896551
CGTTTTT54450.040.74381
ACGTAAG1050.040.7142871
GTGTCGA501.0822987E-940.50000426
CCGTGTA451.929402E-840.036
GCTCGAT451.929402E-840.026
TGTAACG451.929402E-840.01
AAATGCG1300.039.807691
TCATATG750.039.01
ATGGGAC10150.038.7931025
CACGGGC1750.038.5714264