##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1912734
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.299068767533804	31.0	31.0	33.0	30.0	34.0
2	31.55404776618181	31.0	31.0	34.0	30.0	34.0
3	31.623939345460478	31.0	31.0	34.0	30.0	34.0
4	35.404269490687156	37.0	35.0	37.0	33.0	37.0
5	35.13067368489293	37.0	35.0	37.0	32.0	37.0
6	35.26980594269773	37.0	35.0	37.0	32.0	37.0
7	35.747049511327766	37.0	35.0	37.0	35.0	37.0
8	35.80253710134289	37.0	35.0	37.0	35.0	37.0
9	37.49128577209377	39.0	37.0	39.0	35.0	39.0
10	36.88439322979568	39.0	37.0	39.0	32.0	39.0
11	36.65160027478991	39.0	35.0	39.0	32.0	39.0
12	35.8441994548118	37.0	35.0	39.0	31.0	39.0
13	35.42078041170387	37.0	35.0	39.0	30.0	39.0
14	36.40860778341369	38.0	35.0	40.0	31.0	41.0
15	36.63943026055897	38.0	35.0	40.0	31.0	41.0
16	36.75784453039471	38.0	35.0	40.0	32.0	41.0
17	36.718212778148974	38.0	35.0	40.0	32.0	41.0
18	36.66768614977305	38.0	35.0	40.0	31.0	41.0
19	36.5960917723008	38.0	35.0	40.0	31.0	41.0
20	36.531345707244185	38.0	35.0	40.0	31.0	41.0
21	36.40122933978274	38.0	35.0	40.0	31.0	41.0
22	36.27395236347553	38.0	35.0	40.0	31.0	41.0
23	36.23790710051685	38.0	35.0	40.0	31.0	41.0
24	36.10869938004971	37.0	34.0	40.0	30.0	41.0
25	35.98693493188284	37.0	34.0	40.0	30.0	41.0
26	35.942350582987494	37.0	34.0	40.0	30.0	41.0
27	35.87822091310135	37.0	34.0	40.0	30.0	41.0
28	35.84686213556093	37.0	34.0	40.0	30.0	41.0
29	35.884027261501075	37.0	34.0	40.0	30.0	41.0
30	35.799752082621005	37.0	34.0	40.0	30.0	41.0
31	35.694852499092924	37.0	34.0	40.0	30.0	41.0
32	35.53482031479547	37.0	34.0	40.0	29.0	41.0
33	35.35989740340267	37.0	34.0	40.0	29.0	41.0
34	35.2289366948044	37.0	34.0	40.0	27.0	41.0
35	35.05286882546135	37.0	34.0	40.0	27.0	41.0
36	34.87802015335117	37.0	34.0	40.0	26.0	41.0
37	34.787881116767934	36.0	33.0	40.0	26.0	41.0
38	34.772491104356384	36.0	33.0	40.0	26.0	41.0
39	34.7004246277841	36.0	33.0	40.0	26.0	41.0
40	34.5377992967135	36.0	33.0	40.0	24.0	41.0
41	34.4831021982147	36.0	33.0	40.0	24.0	41.0
42	34.43256459078994	36.0	33.0	40.0	24.0	41.0
43	34.4026336124103	36.0	33.0	40.0	25.0	41.0
44	34.159473821242266	35.0	33.0	40.0	24.0	41.0
45	34.14888844972693	35.0	33.0	40.0	24.0	41.0
46	34.105532708677735	35.0	33.0	40.0	24.0	41.0
47	34.08150689013736	35.0	33.0	40.0	24.0	41.0
48	33.95867904266876	35.0	33.0	39.0	23.0	41.0
49	33.88876289123318	35.0	33.0	39.0	24.0	41.0
50	33.81614327972421	35.0	33.0	39.0	24.0	41.0
51	33.738680862054004	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	3.0
13	13.0
14	23.0
15	58.0
16	163.0
17	424.0
18	993.0
19	1980.0
20	3398.0
21	5558.0
22	8252.0
23	11795.0
24	16579.0
25	22999.0
26	29794.0
27	35524.0
28	40192.0
29	46658.0
30	55649.0
31	68566.0
32	85822.0
33	111027.0
34	183794.0
35	258661.0
36	142517.0
37	174853.0
38	244317.0
39	362874.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.27453477587579	24.129126161818633	29.93704299709212	13.659296065213459
2	30.81996764840276	27.386871357961954	29.160562838324616	12.63259815531067
3	29.405029659116217	25.825964300315675	31.342727216643816	13.42627882392429
4	28.020728444206043	27.583082645051533	29.705385066611456	14.690803844130967
5	23.943841642382054	32.8425175690922	28.7544425936905	14.459198194835245
6	21.80501836638027	41.062113184582905	26.484184418743013	10.648684030293811
7	85.28321240695256	5.770797193964242	6.908749465424884	2.037240933658313
8	86.40887859995169	5.022548875065744	6.430272060830204	2.1383004641523597
9	81.25886819599589	7.3174314881211915	8.371367895379075	3.052332420503844
10	47.14549958331896	27.25972351618155	13.424083014156698	12.170693886342795
11	45.01875326103891	21.532319705719665	19.416656994647454	14.032270038593971
12	39.07710115468225	22.61887957238173	24.02074726543262	14.283272007503395
13	23.207931683130013	36.62720482827199	23.71490233351841	16.44996115507959
14	17.00471680850552	40.40044250794935	26.724207338814495	15.870633344730631
15	16.51839722616945	24.34583167340571	43.49805043461349	15.637720665811347
16	17.588593081944484	20.14875042739869	42.14747058399129	20.115185906665538
17	18.10727471776002	20.546139714147394	28.387846924872985	32.9587386432196
18	22.274973937829305	24.564105620541067	31.846613277120607	21.31430716450902
19	30.097023423016477	24.17126479688237	24.97200342546324	20.759708354637915
20	30.8284894815484	23.71751639276554	25.355381354647328	20.09861277103873
21	24.21429221209013	27.272323281752715	26.5803817990374	21.933002707119755
22	23.645054670435094	25.83155838710453	24.520346268744113	26.00304067371626
23	22.18959876281804	29.159046683961282	24.884327878314497	23.76702667490618
24	21.08991631873538	24.72518395134922	34.449798037782564	19.73510169213283
25	19.871398741278192	25.997446586927403	32.251583335685986	21.87957133610842
26	18.75435894379459	33.31571457400768	26.306846639417714	21.623079842780022
27	20.593192780595736	31.528377704374993	28.008128678634876	19.870300836394396
28	17.893130984235132	29.32603278866795	34.159271493056536	18.621564734040383
29	18.078363222486765	26.276157583856406	34.099775504591854	21.545703689064972
30	19.875319830148886	29.948806263704203	29.939761618709138	20.23611228743777
31	27.815838480415987	27.815943042785875	23.81543905216303	20.552779424635105
32	29.314008116131152	26.968674159606092	26.173791023738797	17.543526700523962
33	27.06309397961243	28.57773218858451	24.674471201954898	19.684702629848164
34	21.578327148469153	28.28354596091249	27.08991422748798	23.048212663130368
35	22.75470609086261	25.66624528031603	28.75533137383452	22.823717254986843
36	29.06447002040012	27.128340898420795	24.707199223728963	19.09998985745012
37	22.09596316058584	32.24713943496587	27.1671335376482	18.48976386680009
38	22.395691193861772	32.5235500597574	23.288706113866333	21.792052632514505
39	22.925926971549625	30.609588160193734	25.779643170456527	20.684841697800113
40	23.754688315259727	27.43219914530719	25.645175962784162	23.167936576648923
41	19.274138484493925	26.481988608975428	26.227013270010364	28.01685963652029
42	23.213578051103813	27.71127611053079	23.940181959436075	25.134963878929323
43	23.53134309318494	26.869287627030207	25.583431883366952	24.015937396417904
44	21.68560813997137	29.63464862338412	26.765927724398686	21.913815512245822
45	18.885375593260743	34.596865011026104	23.82511107137741	22.69264832433574
46	22.24888562654295	32.54420112780972	25.27256795769825	19.934345287949082
47	23.593348578526864	27.487042108311975	26.214152098514482	22.705457214646678
48	23.53861017789196	25.23675534601257	29.231874374586326	21.99276010150915
49	21.996106097345475	26.297226901388278	29.373660948150658	22.333006053115593
50	20.999313025229853	31.622483837271677	26.031899887804578	21.346303249693893
51	20.764675067207463	31.7777589565512	24.172153577026393	23.285412399214948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1277.0
1	1962.5
2	2648.0
3	7459.0
4	12270.0
5	9409.5
6	6549.0
7	6798.5
8	7048.0
9	7548.0
10	8048.0
11	8305.5
12	8563.0
13	8394.5
14	8226.0
15	7962.5
16	7699.0
17	7301.5
18	6904.0
19	6902.0
20	6900.0
21	7220.5
22	7541.0
23	7802.5
24	8064.0
25	10190.5
26	14777.5
27	17238.0
28	24071.5
29	30905.0
30	32964.0
31	35023.0
32	38198.5
33	41374.0
34	45303.0
35	49232.0
36	52285.5
37	55339.0
38	62311.5
39	69284.0
40	94722.0
41	120160.0
42	144558.0
43	168956.0
44	170655.5
45	172355.0
46	162224.0
47	152093.0
48	147113.5
49	142134.0
50	134921.5
51	127709.0
52	117923.0
53	108137.0
54	103888.5
55	99640.0
56	98301.5
57	96963.0
58	91020.5
59	85078.0
60	79779.5
61	74481.0
62	64896.0
63	55311.0
64	46536.5
65	37762.0
66	31160.5
67	24559.0
68	20341.0
69	16123.0
70	13440.0
71	10757.0
72	9494.0
73	8231.0
74	6388.5
75	3548.0
76	2550.0
77	2079.0
78	1608.0
79	1119.0
80	630.0
81	438.0
82	246.0
83	201.0
84	156.0
85	110.5
86	65.0
87	43.0
88	21.0
89	14.0
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1912734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.501389379125733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.63289782599215	14.286891309990748
2	6.727545379695657	2.354827825115839
3	2.131789881895836	1.1192785439261843
4	1.0296432752002256	0.7208075152350983
5	0.6376105416956152	0.5579535181225121
6	0.4048832853965348	0.42516120184946676
7	0.327386948064133	0.40108085189998033
8	0.26271451937998896	0.3678295279375248
9	0.23271716129193576	0.36655862894774766
>10	3.3068550699574817	14.710012689012517
>50	1.7492698539406486	22.33435512639983
>100	1.5488257325928398	40.06713491355914
>500	0.0048372460904659124	0.5333487223169547
>1k	0.0021162951645788365	0.7674824620245465
>5k	9.069836419623586E-4	0.9872771636619105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6868	0.35906717818578016	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6353	0.3321423679403409	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5438	0.28430508371786145	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4417	0.23092599389146637	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2134	0.11156804866750943	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2020	0.10560799358405298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019971412648073388	0.0
2	0.0	0.0	0.0	0.09123066772483784	0.0
3	0.0	0.0	0.0	0.14732837916824817	0.0
4	0.0	0.0	0.0	0.22951440189801614	0.0
5	0.0	0.0	0.0	0.38128668178638536	0.0
6	0.0	0.0	0.0	0.5415807948203984	0.0
7	0.0	0.0	0.0	0.6333865555796049	0.0
8	0.0	0.0	0.0	0.8419362023156383	0.0
9	0.0	0.0	0.0	0.9306050919782887	0.0
10	0.0	0.0	0.0	1.0791882195851592	0.0
11	0.0	0.0	0.0	1.2617018362197776	0.0
12	0.0	0.0	0.0	1.4129513042587207	0.0
13	0.0	0.0	0.0	1.4743816965662764	0.0
14	0.0	0.0	0.0	1.501097382071945	0.0
15	0.0	0.0	0.0	1.5416675815874032	0.0
16	0.0	0.0	0.0	1.6310161266543073	0.0
17	0.0	0.0	0.0	1.7275271940583479	0.0
18	0.0	0.0	0.0	1.8613147463264625	0.0
19	0.0	0.0	0.0	1.9273981640939095	0.0
20	0.0	0.0	0.0	1.996043359923544	0.0
21	0.0	0.0	0.0	2.0791181627973363	0.0
22	0.0	0.0	0.0	2.1615655914518173	0.0
23	0.0	0.0	0.0	2.254992068944244	0.0
24	0.0	0.0	0.0	2.330015569336876	0.0
25	0.0	0.0	0.0	2.3924915853432833	0.0
26	0.0	0.0	0.0	2.457215692302223	0.0
27	0.0	0.0	0.0	2.517809585650697	0.0
28	0.0	0.0	0.0	2.5819063183903253	0.0
29	0.0	0.0	0.0	2.6479374549728294	0.0
30	0.0	0.0	0.0	2.741677619574912	0.0
31	0.0	0.0	0.0	2.82527523429813	0.0
32	0.0	0.0	0.0	2.904219823561457	0.0
33	0.0	0.0	0.0	2.979347886323974	0.0
34	0.0	0.0	0.0	3.062056720903168	0.0
35	0.0	0.0	0.0	3.165050655240091	0.0
36	0.0	0.0	0.0	3.251105485655611	0.0
37	0.0	0.0	0.0	3.3385719080645817	0.0
38	0.0	0.0	0.0	3.422796897007111	0.0
39	0.0	0.0	0.0	3.508590321497919	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTCA	25	3.8917802E-5	45.000004	1
TATTACG	40	6.8193913E-9	45.0	1
TCGTACA	35	1.212502E-7	45.0	34
TCGTAAT	20	7.0344645E-4	45.0	45
TCGACGA	35	1.212502E-7	45.0	2
GTACGAC	20	7.0344645E-4	45.0	37
CAAATCG	60	0.0	44.999996	1
TATCGTG	30	2.1662618E-6	44.999996	1
GCGATAT	110	0.0	42.954544	9
TACGTAG	145	0.0	41.89655	1
CGTTTTT	5445	0.0	40.7438	1
ACGTAAG	105	0.0	40.714287	1
GTGTCGA	50	1.0822987E-9	40.500004	26
CCGTGTA	45	1.929402E-8	40.0	36
GCTCGAT	45	1.929402E-8	40.0	26
TGTAACG	45	1.929402E-8	40.0	1
AAATGCG	130	0.0	39.80769	1
TCATATG	75	0.0	39.0	1
ATGGGAC	1015	0.0	38.793102	5
CACGGGC	175	0.0	38.571426	4
>>END_MODULE