##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2785771
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291186533279298	31.0	31.0	33.0	30.0	34.0
2	31.528745184008304	31.0	31.0	34.0	30.0	34.0
3	31.620089375616303	31.0	31.0	34.0	30.0	34.0
4	35.397611289657334	37.0	35.0	37.0	33.0	37.0
5	35.13041488334827	37.0	35.0	37.0	32.0	37.0
6	35.2785878666983	37.0	35.0	37.0	32.0	37.0
7	35.76394254947733	37.0	35.0	37.0	35.0	37.0
8	35.82051791048151	37.0	35.0	37.0	35.0	37.0
9	37.551418978803355	39.0	37.0	39.0	35.0	39.0
10	36.87877754488793	39.0	37.0	39.0	32.0	39.0
11	36.594626406836746	39.0	35.0	39.0	32.0	39.0
12	35.85905517718434	37.0	35.0	39.0	31.0	39.0
13	35.44552549366046	37.0	35.0	39.0	30.0	39.0
14	36.47126307223386	38.0	35.0	41.0	31.0	41.0
15	36.688419471665114	38.0	35.0	41.0	31.0	41.0
16	36.79890055571689	38.0	35.0	41.0	32.0	41.0
17	36.74062081915563	38.0	35.0	41.0	31.0	41.0
18	36.70912289631847	38.0	35.0	40.0	31.0	41.0
19	36.66239112978059	38.0	35.0	40.0	31.0	41.0
20	36.59544772344891	38.0	35.0	40.0	31.0	41.0
21	36.46053426502035	38.0	35.0	40.0	31.0	41.0
22	36.355258203204784	38.0	35.0	40.0	31.0	41.0
23	36.32022050627995	38.0	35.0	40.0	31.0	41.0
24	36.20943609507027	38.0	35.0	40.0	30.0	41.0
25	36.083002515282125	38.0	34.0	40.0	30.0	41.0
26	36.0147592892596	38.0	34.0	40.0	30.0	41.0
27	35.96896693949359	38.0	34.0	40.0	30.0	41.0
28	35.938324794105476	37.0	35.0	40.0	30.0	41.0
29	35.949272930187014	37.0	35.0	40.0	30.0	41.0
30	35.84683701567717	37.0	34.0	40.0	30.0	41.0
31	35.75274026472384	37.0	34.0	40.0	30.0	41.0
32	35.60654985639523	37.0	34.0	40.0	29.0	41.0
33	35.45841671838784	37.0	34.0	40.0	29.0	41.0
34	35.333461005947726	37.0	34.0	40.0	28.0	41.0
35	35.17691619303956	37.0	34.0	40.0	27.0	41.0
36	35.04178807231463	37.0	34.0	40.0	27.0	41.0
37	34.977686967090975	37.0	34.0	40.0	27.0	41.0
38	34.95557603263154	37.0	34.0	40.0	27.0	41.0
39	34.87830622115027	36.0	34.0	40.0	26.0	41.0
40	34.69315281119661	36.0	33.0	40.0	25.0	41.0
41	34.62202743872343	36.0	33.0	40.0	25.0	41.0
42	34.56821468814199	36.0	33.0	40.0	26.0	41.0
43	34.542513006273666	36.0	33.0	40.0	26.0	41.0
44	34.32782845395404	35.0	33.0	40.0	24.0	41.0
45	34.30486174204556	35.0	33.0	40.0	24.0	41.0
46	34.24896267496503	35.0	33.0	40.0	24.0	41.0
47	34.219683168501646	35.0	33.0	40.0	25.0	41.0
48	34.10181885014956	35.0	33.0	39.0	24.0	41.0
49	34.024857032397854	35.0	33.0	39.0	24.0	41.0
50	33.96325433784758	35.0	33.0	39.0	24.0	41.0
51	33.883971439145576	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	14.0
14	31.0
15	78.0
16	230.0
17	669.0
18	1475.0
19	2859.0
20	4768.0
21	7661.0
22	11271.0
23	16459.0
24	22708.0
25	31439.0
26	41248.0
27	49357.0
28	57397.0
29	66702.0
30	80656.0
31	99019.0
32	123429.0
33	160851.0
34	264039.0
35	360871.0
36	213487.0
37	266747.0
38	367872.0
39	534116.0
40	314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.638705047902356	24.734875910475054	28.130129863509957	12.496289178112631
2	30.647170926827798	26.46613809964997	29.570233877802593	13.316457095719642
3	30.287629528773184	25.524818802406944	30.73235380797632	13.455197860843551
4	26.975261067761853	27.865212180039205	30.181554765269652	14.977971986929292
5	24.175712935485365	32.470185094180394	28.894837371772482	14.459264598561763
6	22.346847605205166	41.81693326551249	25.49635271528062	10.339866414001724
7	87.35606767390428	4.953494023737055	5.796743522708795	1.8936947796498707
8	88.65735912966285	3.884526043239017	5.574219847934378	1.8838949791637576
9	83.7946119763613	6.304861383078508	6.950320037074118	2.9502066034860728
10	48.64226097550732	27.144801205842118	12.531862812844272	11.681075005806292
11	43.52479080297699	23.14174424243773	19.073893726368752	14.259571228216533
12	40.56912790031916	21.550694583294895	22.990260147011366	14.889917369374583
13	24.039233662781324	34.2408977622353	23.253562478753636	18.466306096229733
14	17.772099716739103	37.547845820779955	26.418718552242808	18.261335910238135
15	17.814924485896363	23.942456145892823	40.70162263875961	17.5409967294512
16	19.408343327574304	20.489803361439257	40.06352280930486	20.038330501681582
17	19.407086942896598	20.45580918173102	27.994153144677004	32.14295073069538
18	22.921087196327335	24.927677113445434	29.886842816584707	22.26439287364252
19	30.194980132968574	25.24083996854013	23.87658569207591	20.687594206415387
20	32.882638235519	23.692327904913935	23.59651959906252	19.828514260504544
21	25.995927159841926	27.134283471254456	25.147293155108585	21.722496213795033
22	24.478142675761934	25.48827595663822	24.018413573836472	26.01516779376338
23	24.328309828769125	28.624212112194435	23.804935868741545	23.242542190294895
24	22.507054599965322	24.438476816651477	32.86720265233575	20.187265931047456
25	22.14184152250849	24.92437461657832	30.60129493773896	22.332488923174232
26	20.182886533028018	32.39228924416257	25.554936137966834	21.869888084842582
27	21.71872705976191	31.0726186754044	26.339027866971122	20.869626397862568
28	19.047078887675976	29.308367414263415	32.46487238182895	19.179681316231665
29	20.35745220981911	25.801510605143065	31.944477848322776	21.896559336715043
30	22.250823919123288	28.44777980673932	28.251029966210435	21.050366307926964
31	28.660539577732703	27.293808428618142	23.141528862207267	20.90412313144189
32	30.57322371436848	26.568838572876235	24.054059001978267	18.803878710777017
33	28.579377127552842	27.794423877626695	23.810715238258993	19.81548375656147
34	22.59356565920171	28.732045814246753	25.76704976826882	22.907338758282716
35	23.50605990226763	25.441466653217372	28.183867231010733	22.86860621350427
36	28.958123262823825	27.989486573016947	23.284505438530303	19.76788472562892
37	23.690209999314373	31.702677642921834	25.180748884240668	19.42636347352313
38	23.535279820200582	31.54789105062835	22.321648118240876	22.595181010930187
39	24.84141733114459	29.86458685943676	23.711712125655698	21.582283683762952
40	25.013111271529496	26.58165369658884	25.018531673996176	23.386703357885484
41	19.912009996514428	26.250039935084395	26.19583591041762	27.642114157983556
42	23.742332015086667	27.486035284307285	22.61022173035759	26.16141097024845
43	23.534705472919345	27.404621557191888	24.408072307450972	24.652600662437795
44	22.168153807330178	29.674513806052254	25.563946210941246	22.593386175676322
45	20.65302567942591	32.828398314147144	23.047623081724954	23.470952924701994
46	22.32197118858657	32.61158221548002	23.878954874611015	21.18749172132239
47	24.5025883319196	27.43193894975574	25.355099180801293	22.710373537523367
48	23.746460136170562	24.60011967961473	28.342889634503337	23.310530549711373
49	22.247521422256174	25.96189708342861	28.54200865756733	23.24857283674789
50	20.58467835295866	31.098607889880398	25.5022397749133	22.814473982247645
51	20.603775400059803	31.10395649893692	23.97731184652292	24.314956254480357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1253.0
1	2626.5
2	4000.0
3	9968.0
4	15936.0
5	12443.0
6	8950.0
7	9109.0
8	9268.0
9	9517.0
10	9766.0
11	9869.5
12	9973.0
13	9505.5
14	9038.0
15	8733.0
16	8428.0
17	8241.0
18	8054.0
19	8095.0
20	8136.0
21	8770.0
22	9404.0
23	10564.0
24	11724.0
25	14758.0
26	20711.5
27	23631.0
28	29899.5
29	36168.0
30	40668.0
31	45168.0
32	50396.5
33	55625.0
34	60676.5
35	65728.0
36	69170.0
37	72612.0
38	85794.5
39	98977.0
40	126520.5
41	154064.0
42	180842.5
43	207621.0
44	216318.5
45	225016.0
46	217587.0
47	210158.0
48	200262.0
49	190366.0
50	187558.5
51	184751.0
52	179366.5
53	173982.0
54	161136.0
55	148290.0
56	150643.0
57	152996.0
58	156705.0
59	160414.0
60	147269.0
61	134124.0
62	115791.0
63	97458.0
64	84460.5
65	71463.0
66	59850.5
67	48238.0
68	40646.5
69	33055.0
70	27998.0
71	22941.0
72	19987.0
73	17033.0
74	13971.5
75	8605.0
76	6300.0
77	4806.5
78	3313.0
79	2695.5
80	2078.0
81	1533.5
82	989.0
83	650.0
84	311.0
85	259.5
86	208.0
87	122.5
88	37.0
89	26.5
90	16.0
91	10.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2785771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.38622780834254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02479648349396	12.466659768196294
2	7.401779458974085	2.277709298857713
3	2.3098561313918977	1.066199179262776
4	1.1331381269400005	0.6973888543766965
5	0.6335682867062739	0.4874112995702005
6	0.4535876424044548	0.4187401678250373
7	0.32549908178476056	0.3505742116622652
8	0.2430074724166868	0.29911746637861275
9	0.21248098507875599	0.29423527572264946
>10	2.7854280967767187	10.710745849932723
>50	1.4765101041335633	16.548973813793648
>100	1.9849608366248075	51.772286360469245
>500	0.011362924263951869	1.1920301206108794
>1k	0.0037876414213172897	1.1491597245477203
>5k	2.367275888323306E-4	0.26876860879358566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7379	0.2648817867656746	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4725	0.16961193149042042	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4281	0.1536737944360825	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3059	0.1098080208315759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019204737216375646	0.0
2	0.0	0.0	0.0	0.06429099879351174	0.0
3	0.0	0.0	0.0	0.09577240914633686	0.0
4	0.0	0.0	0.0	0.15198664929744762	0.0
5	0.0	0.0	0.0	0.24646677706100034	0.0
6	0.0	0.0	0.0	0.3469057578673911	0.0
7	0.0	0.0	0.0	0.4084327103699478	0.0
8	0.0	0.0	0.0	0.5734857603155464	0.0
9	0.0	0.0	0.0	0.6372742052379754	0.0
10	0.0	0.0	0.0	0.7516052109092959	0.0
11	0.0	0.0	0.0	0.888120380318411	0.0
12	0.0	0.0	0.0	1.0071179576497853	0.0
13	0.0	0.0	0.0	1.0584143492053009	0.0
14	0.0	0.0	0.0	1.0774754996013671	0.0
15	3.589670507733766E-5	0.0	0.0	1.107987698917104	0.0
16	3.589670507733766E-5	0.0	0.0	1.1715966603141463	0.0
17	7.179341015467531E-5	0.0	0.0	1.2432464836485124	0.0
18	7.179341015467531E-5	0.0	0.0	1.3422136995467322	0.0
19	7.179341015467531E-5	0.0	0.0	1.3889153128523486	0.0
20	1.0769011523201297E-4	0.0	0.0	1.4402834978180188	0.0
21	1.0769011523201297E-4	0.0	0.0	1.5079846835938775	0.0
22	1.0769011523201297E-4	0.0	0.0	1.5821831729887346	0.0
23	1.0769011523201297E-4	0.0	0.0	1.6688378190454276	0.0
24	1.0769011523201297E-4	0.0	0.0	1.7344210992217235	0.0
25	1.0769011523201297E-4	0.0	0.0	1.7886251238885034	0.0
26	1.0769011523201297E-4	0.0	0.0	1.8417163506978858	0.0
27	1.0769011523201297E-4	0.0	0.0	1.8949152676225003	0.0
28	1.0769011523201297E-4	0.0	0.0	1.9532474133731739	0.0
29	1.4358682030935063E-4	0.0	0.0	2.013661568018333	0.0
30	1.4358682030935063E-4	0.0	0.0	2.0889369585655104	0.0
31	1.4358682030935063E-4	0.0	0.0	2.1611611291811137	0.0
32	1.7948352538668829E-4	0.0	0.0	2.2309802205565354	0.0
33	1.7948352538668829E-4	0.0	0.0	2.295917360041439	0.0
34	1.7948352538668829E-4	0.0	0.0	2.367315906440264	0.0
35	1.7948352538668829E-4	0.0	0.0	2.460575546231187	0.0
36	1.7948352538668829E-4	0.0	0.0	2.544035385535997	0.0
37	1.7948352538668829E-4	0.0	0.0	2.626956774264647	0.0
38	1.7948352538668829E-4	0.0	0.0	2.7121755521182465	0.0
39	2.1538023046402594E-4	0.0	0.0	2.8010198971846574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	25	3.89224E-5	45.000004	15
ATACGTA	25	3.89224E-5	45.000004	31
ACAATCG	20	7.035019E-4	45.0	31
TATTACG	35	1.2127566E-7	45.0	1
CGTAAAT	70	0.0	45.0	16
TCCGATT	30	2.16662E-6	44.999996	13
TCTAACG	80	0.0	42.1875	1
ATTGGGC	750	0.0	40.5	4
CGTTTTT	6840	0.0	40.19737	1
CGGTCTA	345	0.0	39.782608	31
CGCATAT	80	0.0	39.375	15
TCACGAC	355	0.0	39.295776	25
CGGGTAT	180	0.0	38.75	6
ACCCGCT	950	0.0	38.605263	34
ACTACGT	35	6.2514937E-6	38.571426	44
TTACGAG	35	6.2514937E-6	38.571426	1
TTTAGCG	240	0.0	38.437496	1
CGAATAT	575	0.0	38.347824	14
AACGGGA	705	0.0	38.297874	4
ACGTAGG	235	0.0	38.29787	2
>>END_MODULE