##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1599612
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90242758869026	31.0	31.0	34.0	30.0	34.0
2	32.26710289745263	33.0	31.0	34.0	30.0	34.0
3	32.25022442942414	34.0	31.0	34.0	30.0	34.0
4	35.96113870113503	37.0	35.0	37.0	35.0	37.0
5	35.92615709309508	37.0	35.0	37.0	35.0	37.0
6	35.98062217587765	37.0	35.0	37.0	35.0	37.0
7	36.18159278625067	37.0	35.0	37.0	35.0	37.0
8	36.07687364185815	37.0	35.0	37.0	35.0	37.0
9	37.740910295746716	39.0	38.0	39.0	35.0	39.0
10	37.486799298829965	39.0	37.0	39.0	35.0	39.0
11	37.37740151986857	39.0	37.0	39.0	34.0	39.0
12	37.065429616682046	39.0	35.0	39.0	33.0	39.0
13	36.931401489861294	39.0	35.0	39.0	33.0	39.0
14	38.16476433035011	40.0	37.0	41.0	33.0	41.0
15	38.265674426048314	40.0	37.0	41.0	33.0	41.0
16	38.33230558410415	40.0	37.0	41.0	34.0	41.0
17	38.26087138631118	40.0	36.0	41.0	34.0	41.0
18	38.1160962783475	40.0	36.0	41.0	34.0	41.0
19	37.97731887482715	40.0	36.0	41.0	34.0	41.0
20	37.79973331032775	40.0	35.0	41.0	34.0	41.0
21	37.71716578770352	40.0	35.0	41.0	33.0	41.0
22	37.62698516890346	40.0	35.0	41.0	33.0	41.0
23	37.592056073597846	39.0	35.0	41.0	33.0	41.0
24	37.518118143649836	39.0	35.0	41.0	33.0	41.0
25	37.50637279540288	39.0	35.0	41.0	33.0	41.0
26	37.44892323888543	39.0	35.0	41.0	33.0	41.0
27	37.39629547665309	39.0	35.0	41.0	33.0	41.0
28	37.32168988479707	39.0	35.0	41.0	33.0	41.0
29	37.27660645206463	39.0	35.0	41.0	33.0	41.0
30	37.12105185507485	39.0	35.0	41.0	32.0	41.0
31	36.95654196142564	39.0	35.0	41.0	32.0	41.0
32	36.712400257062335	39.0	35.0	41.0	31.0	41.0
33	36.458575579578046	39.0	35.0	41.0	30.0	41.0
34	36.13095675701358	39.0	35.0	41.0	29.0	41.0
35	35.982189430937005	39.0	35.0	41.0	28.0	41.0
36	35.85297934749177	39.0	35.0	41.0	27.0	41.0
37	35.816888095363126	39.0	35.0	41.0	27.0	41.0
38	35.75331705438569	39.0	35.0	41.0	26.0	41.0
39	35.6527289117611	39.0	35.0	41.0	25.0	41.0
40	35.52716096153317	39.0	35.0	41.0	24.0	41.0
41	35.44800864209571	39.0	35.0	41.0	24.0	41.0
42	35.403983591020825	39.0	35.0	41.0	24.0	41.0
43	35.28905947192194	39.0	35.0	41.0	23.0	41.0
44	35.145993528430644	39.0	35.0	41.0	23.0	41.0
45	35.11283173669615	38.0	35.0	41.0	23.0	41.0
46	35.05384055633491	38.0	35.0	40.0	23.0	41.0
47	35.0368876952661	38.0	35.0	40.0	23.0	41.0
48	34.930600045511035	38.0	35.0	40.0	23.0	41.0
49	34.84268372580351	38.0	34.0	40.0	23.0	41.0
50	34.72458571203517	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	6.0
13	13.0
14	19.0
15	28.0
16	82.0
17	203.0
18	452.0
19	933.0
20	1629.0
21	2836.0
22	4350.0
23	6654.0
24	10205.0
25	16487.0
26	25886.0
27	32831.0
28	32905.0
29	30840.0
30	31507.0
31	34235.0
32	40805.0
33	52596.0
34	91099.0
35	161461.0
36	92385.0
37	121335.0
38	218849.0
39	588975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.634998987254406	24.91810514049657	28.887255159376146	16.559640712872874
2	32.51988607237255	27.424275386781293	29.983270943203728	10.07256759764243
3	29.399441864652175	25.100024255882055	35.00811446775843	10.492419411707338
4	26.837070489593728	26.147903366566393	34.841261505915185	12.173764637924696
5	20.94933021257655	33.20942828635944	33.6327184342203	12.20852306684371
6	20.028231846222706	40.805520338682136	31.008644596314603	8.157603218780554
7	79.23865287332178	5.9822006836657895	12.913193949532761	1.8659524934796687
8	79.95076306004206	5.1501864202069	12.756405928437648	2.1426445913133936
9	71.55910308249751	10.223166617904841	14.694188340672612	3.5235419589250396
10	34.49780321728019	35.18321943071195	21.233586644761353	9.085390707246507
11	32.35390832276827	23.842906904924444	32.28976776868391	11.51341700362338
12	31.45687829298605	23.915361975279005	31.3739831909238	13.253776540811145
13	18.728104065235822	33.658224619470225	32.429676696598925	15.183994618695035
14	14.243704098243823	35.94515419989347	34.348829591175864	15.46231211068684
15	15.413050164664932	27.709782122164622	43.68621890808521	13.190948805085235
16	16.514754828045803	26.429159071074736	40.30421127123328	16.75187482964619
17	15.215877350257436	28.550048386733785	33.811324246129686	22.422750016879093
18	16.404728146575547	31.128736218532993	36.391887532726685	16.074648102164772
19	22.421874804640122	27.609382775323017	32.741314768831444	17.227427651205417
20	21.9660767736176	28.273418804059986	34.24786760789492	15.5126368144275
21	17.325326391649977	31.522769271548352	35.29318359702228	15.858720739779397
22	18.5893829253594	29.052482727061314	32.53764037779162	19.820493969787673
23	18.86076123459939	29.473959935284306	33.090899543139216	18.57437928697709
24	15.186120134132528	28.7582238692883	41.42054448203689	14.635111514542276
25	14.629047544029428	33.613401249803076	36.15733065268328	15.60022055348422
26	15.175305011465282	34.69978969900201	32.676361517668035	17.448543771864678
27	17.870896192326637	33.22149371222521	32.761194589687996	16.146415505760146
28	13.888367929222836	33.280945629315106	37.37049984621271	15.460186595249347
29	14.356606477070692	29.12518785805558	36.801236799923984	19.71696886494975
30	14.977006924179111	33.88577980160189	34.58601210793618	16.551201166282823
31	21.01609640337782	30.690192371650127	32.239443064943245	16.054268160028805
32	19.668332070527104	30.807095720712276	32.23975564074288	17.284816568017742
33	18.929777971158007	33.72086480971635	31.202691652725782	16.14666556639985
34	15.64341852899328	32.051272433565146	32.83615026643961	19.46915877100197
35	20.138696133812452	29.151194164584908	32.12241468556125	18.58769501604139
36	19.978407263761465	34.261495912758846	29.74765130544157	16.012445518038124
37	16.641160481416744	35.041810138958695	32.93611200716174	15.380917372462822
38	15.823149613781343	34.652090631978254	32.12047671560354	17.40428303863687
39	19.12763845232469	32.75744368009242	31.353290672988198	16.761627194594688
40	18.074258007566833	31.552526487673262	31.76889145617812	18.60432404858178
41	14.689562218838068	31.991945546795097	33.090336906699875	20.228155327666958
42	16.082087406196003	32.84571508590833	30.98620165390107	20.085995853994593
43	20.053050364713442	30.617799816455495	30.115678051927592	19.213471766903474
44	15.47143932403608	32.55777025928788	32.7792614709067	19.19152894576935
45	15.714310720349683	36.60418901583634	29.435575627089573	18.245924636724407
46	17.1108368779429	34.95916509753616	30.918310190221128	17.011687834299817
47	19.648702310310252	29.881121171884185	32.78007416798573	17.69010234981983
48	17.627024553454213	30.386743785367955	33.98636669392327	17.99986496725456
49	17.011750349459746	33.07745878375506	32.48662800729177	17.42416285949343
50	15.82002385578503	34.858890781014395	31.470569113009905	17.85051625019067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3220.0
1	6283.5
2	9347.0
3	21081.5
4	32816.0
5	24001.5
6	15187.0
7	15149.0
8	15111.0
9	15473.0
10	15835.0
11	15452.5
12	15070.0
13	14078.0
14	13086.0
15	12946.5
16	12807.0
17	11468.5
18	10130.0
19	10361.0
20	10592.0
21	12474.5
22	14357.0
23	18625.0
24	22893.0
25	32032.0
26	41171.0
27	50518.5
28	59866.0
29	74592.5
30	89319.0
31	96162.5
32	103006.0
33	109213.0
34	115420.0
35	107326.5
36	99233.0
37	102321.0
38	105409.0
39	106022.0
40	106635.0
41	110693.0
42	114751.0
43	109446.5
44	104142.0
45	94254.5
46	84367.0
47	79454.0
48	74541.0
49	70984.5
50	67428.0
51	62018.0
52	56608.0
53	48310.5
54	40013.0
55	35040.5
56	30068.0
57	27829.5
58	25591.0
59	25308.5
60	25026.0
61	23054.5
62	21083.0
63	18102.0
64	15121.0
65	12625.0
66	10129.0
67	8676.0
68	7223.0
69	6032.0
70	4841.0
71	4012.5
72	3184.0
73	2713.5
74	2243.0
75	1822.0
76	1401.0
77	1083.5
78	766.0
79	554.5
80	343.0
81	225.5
82	108.0
83	91.0
84	74.0
85	45.0
86	16.0
87	21.5
88	27.0
89	15.0
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1599612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.79764345592753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70355358251162	15.383472490851254
2	8.441180889743336	3.342309792042554
3	3.037070828226533	1.803805362228823
4	1.6434982110227179	1.3014956640913007
5	1.0540855588060438	1.0434205032642094
6	0.7440271335193881	0.883799034657158
7	0.5827956391216638	0.8076586189700079
8	0.4597627609603353	0.7281775372644445
9	0.3939422296741436	0.7019215024789628
>10	4.477225845504264	20.203761139594917
>50	0.7299986935351627	9.982309848550681
>100	0.6416485553641583	27.837344401123115
>500	0.08135811301296218	10.444816193862938
>1k	0.008580738481835857	2.812116716672815
>5k	9.534153868706507E-4	1.6069238380987516
>10k+	3.178051289568835E-4	1.1166673562481066
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17748	1.1095190583716552	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9931	0.6208380532279077	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9255	0.578577805117741	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6354	0.3972213261715966	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4400	0.27506670367564134	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4025	0.25162351870328553	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3315	0.20723775515562523	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2029	0.1268432594904264	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1874	0.1171534097018527	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1842	0.11515292458421167	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	1811	0.11321495462649693	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1696	0.10602571123497448	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1610	0.10064940748131422	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04632373350537505	0.0
2	0.0	0.0	0.0	0.17360459911528545	0.0
3	0.0	0.0	0.0	0.2569998224569458	0.0
4	0.0	0.0	0.0	0.4514844849876095	0.0
5	0.0	0.0	0.0	0.8131346851611516	0.0
6	0.0	0.0	0.0	1.1585309437538602	0.0
7	0.0	0.0	0.0	1.389774520321178	0.0
8	0.0	0.0	0.0	1.9599127788488708	0.0
9	0.0	0.0	0.0	2.1612741089714254	0.0
10	0.0	0.0	0.0	2.52473724878283	0.0
11	0.0	0.0	0.0	2.869320810296497	0.0
12	0.0	0.0	0.0	3.2097783712550294	0.0
13	0.0	0.0	0.0	3.33218305439069	0.0
14	0.0	0.0	0.0	3.3740056963813725	0.0
15	0.0	0.0	0.0	3.4464607667359335	0.0
16	0.0	0.0	0.0	3.609000182544267	0.0
17	0.0	0.0	0.0	3.799108783880091	0.0
18	0.0	0.0	0.0	4.0580465762947515	0.0
19	0.0	0.0	0.0	4.195892503932203	0.0
20	0.0	0.0	0.0	4.347429251593511	0.0
21	0.0	0.0	0.0	4.564481886857563	0.0
22	0.0	0.0	0.0	4.794287614746576	0.0
23	0.0	0.0	0.0	5.0296571918690285	0.0
24	0.0	0.0	0.0	5.210013428256352	0.0
25	0.0	0.0	0.0	5.375366026261369	0.0
26	0.0	0.0	0.0	5.549783322455696	0.0
27	0.0	0.0	0.0	5.709134465107788	0.0
28	0.0	0.0	0.0	5.876862639190003	0.0
29	0.0	0.0	0.0	6.07734875707359	0.0
30	0.0	0.0	0.0	6.330222578975402	0.0
31	0.0	0.0	0.0	6.581345976399277	0.0
32	0.0	0.0	0.0	6.809276249490502	0.0
33	0.0	0.0	0.0	7.001010244984409	0.0
34	0.0	0.0	0.0	7.2135617887337675	0.0
35	0.0	0.0	0.0	7.476313005903932	0.0
36	0.0	0.0	0.0	7.713495522664246	0.0
37	6.251515992628212E-5	0.0	0.0	7.951115645544045	0.0
38	1.2503031985256423E-4	0.0	0.0	8.185797555907307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	65	0.0	44.000004	7
ATTTACG	35	1.4465695E-7	44.0	1
GGTCGTA	35	1.4465695E-7	44.0	23
CATGACG	30	2.5283407E-6	44.0	1
CGTCTAC	25	4.4436245E-5	44.0	20
TAGCACG	25	4.4436245E-5	44.0	1
ACGATTT	20	7.8574085E-4	44.0	15
CTACGAT	25	4.4436245E-5	44.0	13
TTGACGA	50	2.7284841E-11	44.0	18
GATATCG	30	2.5283407E-6	44.0	1
TATTACG	35	1.4465695E-7	44.0	1
CGGTCGT	35	1.4465695E-7	44.0	28
TAGTGCG	20	7.8574085E-4	44.0	1
ATCGCAC	55	1.8189894E-12	44.0	20
TACCGGC	20	7.8574085E-4	44.0	37
TATAGCG	130	0.0	42.307693	1
CTTTACG	120	0.0	42.166664	36
CGTTTTT	10490	0.0	41.797905	1
CACGACG	90	0.0	41.555553	26
GCGTTAT	75	0.0	41.066666	14
>>END_MODULE