FastQCFastQC Report
Sat 18 Jun 2016
SRR3549163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631010
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179771.102200477005046No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA101870.6245823140262782No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA97990.6007933734311868No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA64270.39405031238312455No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA44450.2725305178999516No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA40790.25009043476128284No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33490.2053328918890749No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA19970.12243947002164304No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA19170.11753453381646954No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA18930.1160630529549175No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA18200.1115872986676967No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.10864433694459262No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.10478169968301851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA650.044.0000049
GACGTAT207.8574516E-444.0000046
CGTTCGC207.8574516E-444.00000442
CCGTATC207.8574516E-444.00000433
TACGTCT207.8574516E-444.00000441
TCACGCG600.044.01
CGTCTAC254.443663E-544.020
ATATGCG502.7284841E-1144.01
ATCACGC302.5283734E-644.018
ATCGTAG254.443663E-544.01
GATATCG302.5283734E-644.01
TATTACG502.7284841E-1144.01
ACTCGAT254.443663E-544.029
TCGTAAG302.5283734E-644.01
CTATTCG302.5283734E-644.01
TAGATCG302.5283734E-644.02
AACTCGA254.443663E-544.028
GACGATA254.443663E-544.020
GATTCGA254.443663E-544.09
AGTCGTA454.802132E-1044.06
Produced by FastQC (version 0.11.5)