##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1631010
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828644214321187	31.0	31.0	34.0	30.0	34.0
2	32.229981422554125	33.0	31.0	34.0	30.0	34.0
3	32.31771601645606	34.0	31.0	34.0	30.0	34.0
4	35.98455129030478	37.0	35.0	37.0	35.0	37.0
5	35.87416754035843	37.0	35.0	37.0	35.0	37.0
6	35.96168018589708	37.0	35.0	37.0	35.0	37.0
7	36.15585680038749	37.0	35.0	37.0	35.0	37.0
8	36.05489175418912	37.0	35.0	37.0	35.0	37.0
9	37.70882275399906	39.0	38.0	39.0	35.0	39.0
10	37.476028350531266	39.0	37.0	39.0	35.0	39.0
11	37.35735709774925	39.0	37.0	39.0	34.0	39.0
12	37.04231856334419	39.0	35.0	39.0	33.0	39.0
13	36.87846426447416	39.0	35.0	39.0	33.0	39.0
14	38.10224216896279	40.0	37.0	41.0	33.0	41.0
15	38.22857186651216	40.0	37.0	41.0	33.0	41.0
16	38.28107062495018	40.0	37.0	41.0	34.0	41.0
17	38.217064886174825	40.0	36.0	41.0	34.0	41.0
18	38.087138031035984	40.0	36.0	41.0	34.0	41.0
19	37.95158092225063	40.0	36.0	41.0	34.0	41.0
20	37.7704949693748	40.0	35.0	41.0	34.0	41.0
21	37.68884433571836	40.0	35.0	41.0	33.0	41.0
22	37.607052685146016	40.0	35.0	41.0	33.0	41.0
23	37.562321506305906	39.0	35.0	41.0	33.0	41.0
24	37.48678916744839	39.0	35.0	41.0	33.0	41.0
25	37.4597991428624	39.0	35.0	41.0	33.0	41.0
26	37.42630394663429	39.0	35.0	41.0	33.0	41.0
27	37.360239360886816	39.0	35.0	41.0	33.0	41.0
28	37.274668456968385	39.0	35.0	41.0	33.0	41.0
29	37.238249918761994	39.0	35.0	41.0	33.0	41.0
30	37.079224529585964	39.0	35.0	41.0	32.0	41.0
31	36.91704955824918	39.0	35.0	41.0	32.0	41.0
32	36.67792165590646	39.0	35.0	41.0	31.0	41.0
33	36.40789449482223	39.0	35.0	41.0	30.0	41.0
34	36.142462645845214	39.0	35.0	41.0	29.0	41.0
35	35.96200881662283	39.0	35.0	41.0	28.0	41.0
36	35.83596360537336	39.0	35.0	41.0	27.0	41.0
37	35.7342983795317	39.0	35.0	41.0	26.0	41.0
38	35.685581326907865	39.0	35.0	41.0	26.0	41.0
39	35.59559843287288	39.0	35.0	41.0	25.0	41.0
40	35.494792797101184	39.0	35.0	41.0	24.0	41.0
41	35.4314559690008	39.0	35.0	41.0	24.0	41.0
42	35.36942937198423	39.0	35.0	41.0	24.0	41.0
43	35.28529070943771	39.0	35.0	41.0	23.0	41.0
44	35.13139956223444	39.0	35.0	41.0	23.0	41.0
45	35.09975046137056	38.0	35.0	41.0	23.0	41.0
46	35.05603705679303	38.0	35.0	40.0	23.0	41.0
47	35.01680063273677	38.0	35.0	40.0	23.0	41.0
48	34.92765709590989	38.0	34.0	40.0	23.0	41.0
49	34.832058049919986	38.0	34.0	40.0	23.0	41.0
50	34.68374872011821	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	9.0
12	6.0
13	11.0
14	19.0
15	29.0
16	94.0
17	195.0
18	466.0
19	951.0
20	1800.0
21	2836.0
22	4523.0
23	6977.0
24	10524.0
25	16708.0
26	26310.0
27	33210.0
28	33997.0
29	32231.0
30	32494.0
31	35846.0
32	42651.0
33	54395.0
34	93877.0
35	163650.0
36	95412.0
37	124434.0
38	224630.0
39	592717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.7289409629616	25.01940515386172	28.918338943354115	16.333314939822564
2	32.56822459702883	27.38309391113482	29.904537679106813	10.144143812729537
3	29.397244652086744	25.09984610762656	34.94791570867131	10.554993531615379
4	26.873103169201904	26.193340322867424	34.79616924482376	12.137387263106909
5	20.991348918768125	33.21009681117835	33.56968994672013	12.228864323333395
6	20.05738775360053	40.72513350623234	31.007106026327246	8.21037271383989
7	79.16573166320255	6.0036419151323415	12.950135192304154	1.8804912293609481
8	79.83666562436773	5.2009491051557015	12.800779884856622	2.1616053856199535
9	71.48460156590089	10.22354246755078	14.72474111133592	3.5671148552124143
10	34.50720719063647	35.03859571676446	21.264737800503983	9.189459292095083
11	32.42027945874029	23.83179747518409	32.261666084205494	11.48625698187013
12	31.45382309121342	23.92033157368747	31.383008074751228	13.242837260347883
13	18.788603380727277	33.572571596740666	32.417459120422315	15.221365902109735
14	14.29776641467557	35.88334835470046	34.4124192984715	15.406465932152468
15	15.352021140275044	27.73060864127136	43.75203095014745	13.165339268306141
16	16.584079803312058	26.407869970141203	40.253462578402335	16.7545876481444
17	15.366245455270047	28.495778689278424	33.802980974978695	22.334994880472834
18	16.44110091293125	31.08460401836899	36.38923121256154	16.08506385613822
19	22.483614447489593	27.597930116921415	32.75019773024077	17.168257705348218
20	22.004953985567223	28.3011140336356	34.225295982244134	15.468635998553044
21	17.277944341236413	31.519794483173	35.35232769878787	15.849933476802716
22	18.475177957216694	29.06567096461702	32.63339893685508	19.825752141311213
23	18.8902581835795	29.401720406373965	33.12193058289036	18.58609082715618
24	15.189238569965848	28.699885347116204	41.39539303866929	14.715483044248657
25	14.7181807591615	33.5574276062072	36.0500548739738	15.674336760657507
26	15.165817499586145	34.63087289470941	32.69906377030184	17.504245835402603
27	17.859547151764858	33.194401015321795	32.856818781000726	16.08923305191262
28	13.863434313707456	33.248416625281266	37.41111335920687	15.477035701804404
29	14.426520990061373	29.066100146534968	36.82160133904758	19.685777524356073
30	14.979859105707508	33.846450972097045	34.607513136032274	16.566176786163176
31	20.99288171133224	30.610051440518454	32.33775390708825	16.05931294106106
32	19.620112690909313	30.846469365607813	32.2940999748622	17.23931796862067
33	18.88725391015383	33.66306766972612	31.267926009037346	16.1817524110827
34	15.61265718787745	32.06835028601909	32.83989675109288	19.479095775010578
35	20.16787144162206	29.050036480463028	32.08171623717819	18.700375840736722
36	19.88706384387588	34.189673883054056	29.807542565649502	16.115719707420556
37	16.63153506109711	34.97342137693822	32.94149024224254	15.453553319722136
38	15.849872165100154	34.60389574558096	32.09213922661419	17.454092862704705
39	19.07069852422732	32.73983605250734	31.350390248986827	16.839075174278513
40	18.023678579530475	31.485705176547047	31.850264559996567	18.64035168392591
41	14.725415540064132	31.915622834930502	33.13014635103402	20.228815273971342
42	16.05851588892772	32.78330604962569	31.04291206062501	20.115266000821578
43	20.03586734600033	30.552847622025613	30.150029736175743	19.26125529579831
44	15.439083757916874	32.59845126639322	32.737138337594494	19.225326638095414
45	15.738407489837586	36.46366361947505	29.417784072445908	18.380144818241458
46	17.11326110814771	34.8521468292653	30.94910515570107	17.085486906885915
47	19.507667028405713	29.892214026891313	32.78667819326675	17.813440751436225
48	17.575551345485312	30.3855892974292	33.97416324853925	18.064696108546237
49	17.054340561983068	32.93707579965788	32.492075462443516	17.516508175915536
50	15.838590811828254	34.79353284161348	31.46602412002379	17.90185222653448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3314.0
1	6611.0
2	9908.0
3	21579.0
4	33250.0
5	24252.0
6	15254.0
7	15369.5
8	15485.0
9	15825.0
10	16165.0
11	15618.0
12	15071.0
13	14191.0
14	13311.0
15	13194.0
16	13077.0
17	11698.5
18	10320.0
19	10578.5
20	10837.0
21	12679.5
22	14522.0
23	18930.5
24	23339.0
25	32409.5
26	41480.0
27	50937.5
28	60395.0
29	75779.0
30	91163.0
31	97657.5
32	104152.0
33	110949.5
34	117747.0
35	109250.0
36	100753.0
37	104162.5
38	107572.0
39	107967.5
40	108363.0
41	113196.5
42	118030.0
43	111988.0
44	105946.0
45	96225.0
46	86504.0
47	81361.0
48	76218.0
49	73000.0
50	69782.0
51	63958.5
52	58135.0
53	49974.5
54	41814.0
55	36319.0
56	30824.0
57	28507.0
58	26190.0
59	25633.5
60	25077.0
61	23018.0
62	20959.0
63	18114.0
64	15269.0
65	12778.0
66	10287.0
67	8880.5
68	7474.0
69	6164.0
70	4854.0
71	4043.0
72	3232.0
73	2765.5
74	2299.0
75	1857.0
76	1415.0
77	1061.0
78	707.0
79	503.0
80	299.0
81	206.0
82	113.0
83	91.0
84	69.0
85	41.5
86	14.0
87	16.0
88	18.0
89	10.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1631010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.9624292030976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86172564557987	15.543091858308966
2	8.38577039643782	3.348006957046433
3	3.0837314886643252	1.8467631457147304
4	1.6273509197933935	1.2994351009988552
5	1.0253415532275794	1.023415408264984
6	0.7341522008608224	0.8793276800389475
7	0.56251539853087	0.7860421673177307
8	0.4638667170817017	0.7407925199533418
9	0.3770930928856072	0.6774924752735436
>10	4.434732399838827	20.031709148579665
>50	0.7162933039853538	9.87689624000108
>100	0.6374263631367633	27.856207984900692
>500	0.0791757151687855	10.28208929245616
>1k	0.009587684258720119	3.068099035657212
>5k	6.185602747561367E-4	1.001790456646153
>10k+	6.185602747561367E-4	1.7388405288415045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17977	1.102200477005046	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	10187	0.6245823140262782	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	9799	0.6007933734311868	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6427	0.39405031238312455	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	4445	0.2725305178999516	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4079	0.25009043476128284	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3349	0.2053328918890749	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	1997	0.12243947002164304	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1917	0.11753453381646954	No Hit
GAAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1893	0.1160630529549175	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAA	1820	0.1115872986676967	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1772	0.10864433694459262	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1709	0.10478169968301851	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0496624790773815	0.0
2	0.0	0.0	0.0	0.18632626409402764	0.0
3	0.0	0.0	0.0	0.2685452572332481	0.0
4	0.0	0.0	0.0	0.45407446919393507	0.0
5	0.0	0.0	0.0	0.8175302419972901	0.0
6	0.0	0.0	0.0	1.1649836604312664	0.0
7	0.0	0.0	0.0	1.4056320929975905	0.0
8	0.0	0.0	0.0	1.9934273854850675	0.0
9	0.0	0.0	0.0	2.204952759333174	0.0
10	0.0	0.0	0.0	2.577053482198147	0.0
11	0.0	0.0	0.0	2.9308220059962844	0.0
12	0.0	0.0	0.0	3.2849584000098098	0.0
13	0.0	0.0	0.0	3.409789026431475	0.0
14	6.131170256466852E-5	0.0	0.0	3.4540560756831655	0.0
15	6.131170256466852E-5	0.0	0.0	3.5290402879197553	0.0
16	6.131170256466852E-5	0.0	0.0	3.6910871177981743	0.0
17	6.131170256466852E-5	0.0	0.0	3.8811533957486466	0.0
18	6.131170256466852E-5	0.0	0.0	4.145652080612627	0.0
19	6.131170256466852E-5	0.0	0.0	4.29568181678837	0.0
20	6.131170256466852E-5	0.0	0.0	4.451965346625711	0.0
21	6.131170256466852E-5	6.131170256466852E-5	0.0	4.676182242904703	0.0
22	6.131170256466852E-5	6.131170256466852E-5	0.0	4.924065456373658	0.0
23	6.131170256466852E-5	6.131170256466852E-5	0.0	5.17991919117602	0.0
24	6.131170256466852E-5	6.131170256466852E-5	0.0	5.379917964941969	0.0
25	6.131170256466852E-5	6.131170256466852E-5	0.0	5.556250421517955	0.0
26	6.131170256466852E-5	6.131170256466852E-5	0.0	5.734422229170882	0.0
27	6.131170256466852E-5	6.131170256466852E-5	0.0	5.898492345233935	0.0
28	6.131170256466852E-5	6.131170256466852E-5	0.0	6.0728015156252875	0.0
29	6.131170256466852E-5	6.131170256466852E-5	0.0	6.277030796868198	0.0
30	6.131170256466852E-5	6.131170256466852E-5	0.0	6.542510468973213	0.0
31	6.131170256466852E-5	6.131170256466852E-5	0.0	6.810565232585944	0.0
32	6.131170256466852E-5	6.131170256466852E-5	0.0	7.05072317153175	0.0
33	6.131170256466852E-5	6.131170256466852E-5	0.0	7.2535422836156735	0.0
34	6.131170256466852E-5	6.131170256466852E-5	0.0	7.473712607525399	0.0
35	6.131170256466852E-5	6.131170256466852E-5	0.0	7.747469359476644	0.0
36	6.131170256466852E-5	6.131170256466852E-5	0.0	8.005407692166203	0.0
37	6.131170256466852E-5	6.131170256466852E-5	0.0	8.243910215142764	0.0
38	6.131170256466852E-5	6.131170256466852E-5	0.0	8.490260636047603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAA	65	0.0	44.000004	9
GACGTAT	20	7.8574516E-4	44.000004	6
CGTTCGC	20	7.8574516E-4	44.000004	42
CCGTATC	20	7.8574516E-4	44.000004	33
TACGTCT	20	7.8574516E-4	44.000004	41
TCACGCG	60	0.0	44.0	1
CGTCTAC	25	4.443663E-5	44.0	20
ATATGCG	50	2.7284841E-11	44.0	1
ATCACGC	30	2.5283734E-6	44.0	18
ATCGTAG	25	4.443663E-5	44.0	1
GATATCG	30	2.5283734E-6	44.0	1
TATTACG	50	2.7284841E-11	44.0	1
ACTCGAT	25	4.443663E-5	44.0	29
TCGTAAG	30	2.5283734E-6	44.0	1
CTATTCG	30	2.5283734E-6	44.0	1
TAGATCG	30	2.5283734E-6	44.0	2
AACTCGA	25	4.443663E-5	44.0	28
GACGATA	25	4.443663E-5	44.0	20
GATTCGA	25	4.443663E-5	44.0	9
AGTCGTA	45	4.802132E-10	44.0	6
>>END_MODULE