FastQCFastQC Report
Sat 18 Jun 2016
SRR3549162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4148530
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT607571.4645428621704557No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA341820.8239545091875917No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA315210.7598113066556106No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA147210.3548485849204417No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA146610.35340228948567326No Hit
TAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA82780.19954056015022187No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG81010.19527398861765494No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA68190.1643714761614355No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA67380.16241897732449806No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA62970.1517887058789499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57610.13886846666168498No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA53120.12804535582483434No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC52570.1267195850096299No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA51360.12380288921618018No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG46180.1113165386293458No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA45610.10994255796631577No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA45070.10864089207502417No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT44190.1065196587706971No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG254.4448556E-544.01
ACGTATC254.4448556E-544.011
TCACGAC2650.042.33962625
ACGGGTA4150.041.8795175
CGTTTTT360300.041.7224541
CGACGGT2550.041.41176228
TAGAACG4200.041.3809551
ACGTATA800.041.25000413
TAGCGGA1550.041.161293
CGTAAGT2000.040.713
TGTTATC11900.040.67226824
ACTACGG1950.040.6153872
CGGTCTA2600.040.61538331
ATGGGCG5650.040.495575
CGTAAGG4300.040.4186022
GGTTACG603.6379788E-1240.3333321
CGAGGGT4100.040.24394
GGCGTAA1650.040.00000438
CCTACGC557.8216544E-1140.028
ACATACG28800.039.9513917
Produced by FastQC (version 0.11.5)