##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4148530
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95411073319947	33.0	31.0	34.0	30.0	34.0
2	32.31968480401492	33.0	31.0	34.0	31.0	34.0
3	32.274333077017644	34.0	31.0	34.0	30.0	34.0
4	35.99836881979882	37.0	35.0	37.0	35.0	37.0
5	35.97033623958366	37.0	35.0	37.0	35.0	37.0
6	36.02521326831432	37.0	35.0	37.0	35.0	37.0
7	36.19338343943517	37.0	35.0	37.0	35.0	37.0
8	36.07221473630418	37.0	35.0	37.0	35.0	37.0
9	37.74390326211935	39.0	38.0	39.0	35.0	39.0
10	37.55159490229069	39.0	37.0	39.0	35.0	39.0
11	37.46284009034525	39.0	37.0	39.0	35.0	39.0
12	37.200011811412715	39.0	37.0	39.0	34.0	39.0
13	37.10041219419891	39.0	37.0	39.0	33.0	39.0
14	38.40005230768489	40.0	37.0	41.0	34.0	41.0
15	38.45774768411943	40.0	37.0	41.0	34.0	41.0
16	38.48648677965448	40.0	37.0	41.0	34.0	41.0
17	38.38834792082979	40.0	37.0	41.0	34.0	41.0
18	38.237120618628765	40.0	37.0	41.0	34.0	41.0
19	38.07880670984662	40.0	37.0	41.0	34.0	41.0
20	37.83358659573391	40.0	35.0	41.0	34.0	41.0
21	37.79432545986169	40.0	35.0	41.0	34.0	41.0
22	37.705156525323424	40.0	35.0	41.0	33.0	41.0
23	37.650973959450695	40.0	35.0	41.0	33.0	41.0
24	37.56006247996278	39.0	35.0	41.0	33.0	41.0
25	37.55636116889597	39.0	35.0	41.0	33.0	41.0
26	37.50717748214428	39.0	35.0	41.0	33.0	41.0
27	37.473094324977765	40.0	35.0	41.0	33.0	41.0
28	37.397825012715344	40.0	35.0	41.0	33.0	41.0
29	37.32923035388439	40.0	35.0	41.0	33.0	41.0
30	37.16320648518873	39.0	35.0	41.0	33.0	41.0
31	36.95819193786715	39.0	35.0	41.0	32.0	41.0
32	36.69228473700323	40.0	35.0	41.0	31.0	41.0
33	36.36482030984469	40.0	35.0	41.0	30.0	41.0
34	35.97596787295741	40.0	35.0	41.0	27.0	41.0
35	35.76258771179189	40.0	35.0	41.0	25.0	41.0
36	35.60679517805102	39.0	35.0	41.0	24.0	41.0
37	35.579607475419	39.0	35.0	41.0	24.0	41.0
38	35.514902146061374	39.0	35.0	41.0	23.0	41.0
39	35.42757097092223	39.0	35.0	41.0	23.0	41.0
40	35.296899142587854	39.0	35.0	41.0	23.0	41.0
41	35.20040062383543	39.0	35.0	41.0	23.0	41.0
42	35.16210826485526	39.0	35.0	41.0	22.0	41.0
43	35.06496903722523	39.0	35.0	41.0	22.0	41.0
44	34.92091897611925	39.0	35.0	41.0	21.0	41.0
45	34.882065454510396	39.0	35.0	41.0	21.0	41.0
46	34.82694954598376	38.0	35.0	41.0	20.0	41.0
47	34.796694250734596	38.0	35.0	40.0	20.0	41.0
48	34.704281275536154	38.0	34.0	40.0	20.0	41.0
49	34.607015014957106	38.0	34.0	40.0	20.0	41.0
50	34.51034004816164	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	2.0
11	6.0
12	12.0
13	14.0
14	30.0
15	69.0
16	185.0
17	511.0
18	1134.0
19	2352.0
20	4250.0
21	7054.0
22	11759.0
23	18221.0
24	28951.0
25	48002.0
26	77070.0
27	95604.0
28	93356.0
29	83367.0
30	80203.0
31	85108.0
32	100490.0
33	127719.0
34	214030.0
35	401526.0
36	243013.0
37	306722.0
38	544939.0
39	1572824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.028643881085593	26.07834582370141	26.252961892525782	18.640048402687217
2	32.86983582136323	28.544207225209895	28.202351194278457	10.383605759148422
3	27.859747910705718	25.03156539786382	37.03111704627904	10.077569645151415
4	26.390504588372266	25.647205154596932	36.79884199945523	11.163448257575576
5	21.36498952641056	32.084883079066564	35.002446649777156	11.54768074474573
6	19.326701265267456	40.89795662559991	31.439377321605484	8.335964787527148
7	76.58736950196817	6.967841621007924	14.503643459249421	1.9411454177744887
8	77.12235418328902	6.7505357319339625	14.134211395361731	1.992898689415287
9	68.29081626503846	11.924271971035523	16.24286192940632	3.542049834519698
10	31.516585392898204	37.519747958915566	22.361776340052984	8.601890308133243
11	31.357541104921506	24.569763265542253	32.13432227801173	11.938373351524517
12	30.936307559545188	23.42986551862949	33.248982169587784	12.384844752237539
13	17.944380298563587	35.547748238532684	31.47994590855074	15.027925554352988
14	13.173678387284172	37.47375576408993	33.52628521428072	15.826280634345178
15	15.454775547000985	28.880085235010956	42.66436544993046	13.000773768057602
16	15.795426331736785	26.94836484248637	39.32424256302835	17.931966262748492
17	13.655246557214243	30.567285279364015	34.19659493844808	21.580873224973665
18	15.198949989514357	32.41960405251981	37.2226306667663	15.158815291199534
19	22.015870681904193	28.539530869970807	32.607357304876665	16.83724114324833
20	21.542570500876216	28.45357271129774	35.903247656398776	14.100609131427277
21	16.9837990806382	33.05922820854616	34.350336143163965	15.606636567651675
22	18.412787180037267	30.714011951221277	32.186943326913386	18.68625754182807
23	18.3516811979183	30.83504277418748	32.857397680624224	17.955878347269998
24	15.100891159037054	30.09810704032513	41.30504058063941	13.49596121999841
25	14.120568008427082	35.409868073751426	36.200340843624126	14.269223074197365
26	14.280721122903776	35.36057350434974	33.38654897035818	16.972156402388315
27	16.643172400826316	33.44996902517277	33.17403996114287	16.73281861285805
28	13.261492625098528	33.54540041894358	37.57130839116506	15.62179856479283
29	13.826270992375614	30.04117121004308	35.95379568184393	20.17876211573738
30	13.881471268135941	36.51623587150147	33.91198810180956	15.690304758553031
31	20.598187791820237	33.746628323767695	31.17241528927114	14.48276859514093
32	19.817260571817005	31.898118128590124	31.62462366187541	16.659997637717456
33	17.52276107440467	35.212135382894665	29.871086866914304	17.394016675786364
34	15.115040749373875	33.134989984404115	32.87224631375451	18.8777229524675
35	21.22935111955319	29.44254952959241	32.21112056559793	17.116978785256464
36	19.572667908873743	35.921567398572506	29.854285734947076	14.65147895760667
37	15.601405799162595	36.6862478998585	33.76166979628929	13.950676504689612
38	15.373855317425692	35.03915844889636	33.46443197952046	16.1225542541575
39	18.698575157947513	33.876095870103384	32.01857043338243	15.406758538566672
40	17.965158742976428	34.11994128040535	31.227953033966248	16.686946942651975
41	14.603847627954963	34.972122655494836	32.23900996256505	18.185019753985145
42	15.290211231448247	35.02336972373347	29.618515474155906	20.06790357066238
43	19.39706353816894	31.895924580514063	30.895088139654288	17.81192374166271
44	15.981371714800183	34.34896216250093	30.543083935755554	19.126582186943327
45	14.917766052071457	39.040214244563735	28.243040305843277	17.79897939752153
46	16.7809079360641	39.05583423525923	29.283432926843965	14.879824901832697
47	19.54231980966752	31.859622565101375	32.18289369969604	16.41516392553507
48	17.117412673886896	31.1574702364452	34.385529332076665	17.339587757591243
49	16.613209980402697	34.42161440317414	32.507659339573294	16.457516276849873
50	15.182076542775395	35.2185955025033	32.57826266171391	17.021065293007403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9016.0
1	15567.0
2	22118.0
3	64845.5
4	107573.0
5	78044.0
6	48515.0
7	47670.5
8	46826.0
9	47021.0
10	47216.0
11	47728.5
12	48241.0
13	45055.0
14	41869.0
15	38586.5
16	35304.0
17	33748.5
18	32193.0
19	34299.0
20	36405.0
21	43224.5
22	50044.0
23	57459.5
24	64875.0
25	81388.5
26	97902.0
27	131752.5
28	165603.0
29	208842.0
30	252081.0
31	252429.0
32	252777.0
33	268919.5
34	285062.0
35	288723.0
36	292384.0
37	289719.0
38	287054.0
39	276455.5
40	265857.0
41	273822.5
42	281788.0
43	273986.5
44	266185.0
45	256009.5
46	245834.0
47	222243.5
48	198653.0
49	182278.5
50	165904.0
51	140994.0
52	116084.0
53	99578.5
54	83073.0
55	72929.5
56	62786.0
57	60110.5
58	57435.0
59	56697.0
60	55959.0
61	49168.5
62	42378.0
63	36239.0
64	30100.0
65	24140.5
66	18181.0
67	15733.0
68	13285.0
69	10813.0
70	8341.0
71	7199.5
72	6058.0
73	4683.5
74	3309.0
75	2679.5
76	2050.0
77	1629.0
78	1208.0
79	872.5
80	537.0
81	362.5
82	188.0
83	145.5
84	103.0
85	90.0
86	77.0
87	48.0
88	19.0
89	35.0
90	51.0
91	34.0
92	17.0
93	13.0
94	9.0
95	6.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4148530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.68064541468148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61928237307379	12.9473972660728
2	8.911632169705694	2.9730355257785845
3	3.3904819165566735	1.6966627990491465
4	1.7261806974174232	1.1517523254115045
5	1.0880904144725934	0.9075025191465567
6	0.710125827029072	0.710721427228763
7	0.5459974211201848	0.6375312565325425
8	0.4409441188493347	0.5884185995371936
9	0.34872873709634755	0.5235318368472467
>10	3.5143759136012225	13.110613782361499
>50	0.8941090210676079	10.546303057143552
>100	0.633098706126968	21.761901088418387
>500	0.12445184626830627	14.779233034533496
>1k	0.05045345118985389	12.456256959182674
>5k	0.001316176987561406	1.4075146394580007
>10k+	7.312094375341143E-4	3.801623883298043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60757	1.4645428621704557	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	34182	0.8239545091875917	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	31521	0.7598113066556106	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	14721	0.3548485849204417	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	14661	0.35340228948567326	No Hit
TAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8278	0.19954056015022187	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8101	0.19527398861765494	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6819	0.1643714761614355	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	6738	0.16241897732449806	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	6297	0.1517887058789499	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5761	0.13886846666168498	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5312	0.12804535582483434	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	5257	0.1267195850096299	No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5136	0.12380288921618018	No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4618	0.1113165386293458	No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4561	0.10994255796631577	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4507	0.10864089207502417	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT	4419	0.1065196587706971	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.046594817923457224	0.0
2	0.0	0.0	0.0	0.19488830983505	0.0
3	0.0	0.0	0.0	0.2875476373558827	0.0
4	0.0	0.0	0.0	0.4623806504954767	0.0
5	0.0	0.0	0.0	0.8943408870129902	0.0
6	0.0	0.0	0.0	1.216696034498967	0.0
7	0.0	0.0	0.0	1.4428725355728416	0.0
8	0.0	0.0	0.0	1.926706568350717	0.0
9	0.0	0.0	0.0	2.127259535305277	0.0
10	0.0	0.0	0.0	2.5875189524964264	0.0
11	0.0	0.0	0.0	2.9788864971447717	0.0
12	0.0	0.0	0.0	3.357719481358457	0.0
13	0.0	0.0	0.0	3.4828722463137547	0.0
14	0.0	0.0	0.0	3.5293706445415607	0.0
15	0.0	0.0	0.0	3.6129665206711774	0.0
16	0.0	0.0	0.0	3.765261429952296	0.0
17	0.0	0.0	0.0	3.954292243276534	0.0
18	0.0	0.0	0.0	4.209635702284905	0.0
19	0.0	0.0	0.0	4.353276943881327	0.0
20	2.4104923912807668E-5	0.0	0.0	4.506801204281999	0.0
21	4.8209847825615335E-5	0.0	0.0	4.699375441421419	0.0
22	4.8209847825615335E-5	0.0	0.0	4.9141262085606225	0.0
23	4.8209847825615335E-5	0.0	0.0	5.143484559590988	0.0
24	7.2314771738423E-5	0.0	0.0	5.3226805639588	0.0
25	7.2314771738423E-5	0.0	0.0	5.475553991413826	0.0
26	7.2314771738423E-5	0.0	0.0	5.62806584501016	0.0
27	7.2314771738423E-5	0.0	0.0	5.781493685715181	0.0
28	7.2314771738423E-5	0.0	0.0	5.949697844778753	0.0
29	7.2314771738423E-5	0.0	0.0	6.12163826704881	0.0
30	7.2314771738423E-5	0.0	0.0	6.357529052459546	0.0
31	7.2314771738423E-5	0.0	0.0	6.583898392924723	0.0
32	9.641969565123067E-5	0.0	0.0	6.782426546270607	0.0
33	9.641969565123067E-5	0.0	0.0	6.969914644464425	0.0
34	9.641969565123067E-5	0.0	0.0	7.172444215179834	0.0
35	9.641969565123067E-5	0.0	0.0	7.426461903373002	0.0
36	9.641969565123067E-5	0.0	0.0	7.644973038642603	0.0
37	9.641969565123067E-5	0.0	0.0	7.861748619390483	0.0
38	9.641969565123067E-5	0.0	0.0	8.060156248116803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	25	4.4448556E-5	44.0	1
ACGTATC	25	4.4448556E-5	44.0	11
TCACGAC	265	0.0	42.339626	25
ACGGGTA	415	0.0	41.879517	5
CGTTTTT	36030	0.0	41.722454	1
CGACGGT	255	0.0	41.411762	28
TAGAACG	420	0.0	41.380955	1
ACGTATA	80	0.0	41.250004	13
TAGCGGA	155	0.0	41.16129	3
CGTAAGT	200	0.0	40.7	13
TGTTATC	1190	0.0	40.672268	24
ACTACGG	195	0.0	40.615387	2
CGGTCTA	260	0.0	40.615383	31
ATGGGCG	565	0.0	40.49557	5
CGTAAGG	430	0.0	40.418602	2
GGTTACG	60	3.6379788E-12	40.333332	1
CGAGGGT	410	0.0	40.2439	4
GGCGTAA	165	0.0	40.000004	38
CCTACGC	55	7.8216544E-11	40.0	28
ACATACG	2880	0.0	39.95139	17
>>END_MODULE