FastQCFastQC Report
Sat 18 Jun 2016
SRR3549161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233831
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT616961.457214518009812No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA348600.8233677725917732No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA325200.7680986794229623No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA151750.35842243112679745No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA149900.3540528660685795No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG83800.19792948750198106No Hit
TAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA83460.19712643230209237No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA68970.1629021092244825No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA67820.16018589310721187No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA63950.15104523539083162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57170.1350313699342274No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA55190.13035475435840493No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC53240.1257489965943374No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA50460.11918283937171796No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA47060.1111522873728309No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG46890.11075075977288655No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA45170.10668824523227308No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.10042913852725817No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC254.4448723E-544.012
CGTATCT207.8588794E-444.012
ACGCATC207.8588794E-444.015
TACGACG700.044.01
TCGACAG207.8588794E-444.01
CTTACGT351.4473153E-744.027
GTATACG1600.042.6251
CGTAAGT1500.042.53333313
CGTTTTT368850.041.4889531
CGTATGG1700.041.4117622
GCGCTAA800.041.257
ACGGGTA4550.041.0989045
GTACGCA700.040.8571439
TAGAACG3450.040.8115921
CGACGGT2700.040.7407428
GTACCGG2400.040.3333322
TCACGAC2950.040.27118725
TGTTATC13650.040.13186624
TATAGCG3350.040.0597041
TACCGGG7750.040.0258033
Produced by FastQC (version 0.11.5)