##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4233831
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.888880779605987	31.0	31.0	34.0	30.0	34.0
2	32.28868133848517	33.0	31.0	34.0	31.0	34.0
3	32.35560536072413	34.0	31.0	34.0	30.0	34.0
4	36.03054396833506	37.0	35.0	37.0	35.0	37.0
5	35.93129815526411	37.0	35.0	37.0	35.0	37.0
6	36.01196481390022	37.0	35.0	37.0	35.0	37.0
7	36.175722176912586	37.0	35.0	37.0	35.0	37.0
8	36.05455130353573	37.0	35.0	37.0	35.0	37.0
9	37.714227847072785	39.0	38.0	39.0	35.0	39.0
10	37.54675611756823	39.0	37.0	39.0	35.0	39.0
11	37.45877480702465	39.0	37.0	39.0	35.0	39.0
12	37.18590680638882	39.0	37.0	39.0	34.0	39.0
13	37.062285906074194	39.0	37.0	39.0	33.0	39.0
14	38.3520348355898	40.0	37.0	41.0	33.0	41.0
15	38.433728223918244	40.0	37.0	41.0	34.0	41.0
16	38.451297182150164	40.0	37.0	41.0	34.0	41.0
17	38.35747647933987	40.0	37.0	41.0	34.0	41.0
18	38.217726687720884	40.0	37.0	41.0	34.0	41.0
19	38.0690292078262	40.0	36.0	41.0	34.0	41.0
20	37.82281390069656	40.0	35.0	41.0	34.0	41.0
21	37.78001790813096	40.0	35.0	41.0	34.0	41.0
22	37.69828531181334	40.0	35.0	41.0	33.0	41.0
23	37.627535676317734	40.0	35.0	41.0	33.0	41.0
24	37.54223420821474	39.0	35.0	41.0	33.0	41.0
25	37.53202241657733	39.0	35.0	41.0	33.0	41.0
26	37.497016768028764	39.0	35.0	41.0	33.0	41.0
27	37.45423447464011	40.0	35.0	41.0	33.0	41.0
28	37.37454848811868	39.0	35.0	41.0	33.0	41.0
29	37.30571106876963	40.0	35.0	41.0	33.0	41.0
30	37.14097964703834	39.0	35.0	41.0	33.0	41.0
31	36.95073917688259	39.0	35.0	41.0	32.0	41.0
32	36.689654830341595	40.0	35.0	41.0	31.0	41.0
33	36.34620536341673	40.0	35.0	41.0	30.0	41.0
34	36.0160580334926	40.0	35.0	41.0	28.0	41.0
35	35.78187910665305	40.0	35.0	41.0	25.0	41.0
36	35.631385145037676	39.0	35.0	41.0	24.0	41.0
37	35.53150184785363	39.0	35.0	41.0	24.0	41.0
38	35.47533498620989	39.0	35.0	41.0	23.0	41.0
39	35.39833592791021	39.0	35.0	41.0	23.0	41.0
40	35.28417289211591	39.0	35.0	41.0	23.0	41.0
41	35.20649855887021	39.0	35.0	41.0	23.0	41.0
42	35.14742723552263	39.0	35.0	41.0	22.0	41.0
43	35.083562617402535	39.0	35.0	41.0	22.0	41.0
44	34.924985669007576	39.0	35.0	41.0	21.0	41.0
45	34.885148698660856	39.0	35.0	41.0	21.0	41.0
46	34.83890311162632	38.0	35.0	41.0	20.0	41.0
47	34.79476790641856	38.0	35.0	41.0	20.0	41.0
48	34.724557026484995	38.0	34.0	40.0	20.0	41.0
49	34.61363809750554	38.0	34.0	40.0	20.0	41.0
50	34.49436479632749	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	11.0
12	7.0
13	17.0
14	31.0
15	69.0
16	186.0
17	490.0
18	1177.0
19	2536.0
20	4366.0
21	7577.0
22	12002.0
23	18933.0
24	29572.0
25	48838.0
26	77569.0
27	96780.0
28	95660.0
29	85741.0
30	81769.0
31	87557.0
32	103698.0
33	131727.0
34	222883.0
35	406642.0
36	250337.0
37	315356.0
38	560267.0
39	1592021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.199724788259147	26.15749187910429	26.217579303472434	18.42520402916413
2	32.922169070990314	28.451560773209888	28.24316794883877	10.383102206961023
3	27.85949651745665	25.02492423528478	36.99727268282555	10.118306564433016
4	26.389126065731013	25.719566983188514	36.74856176356591	11.14274518751457
5	21.407325894680255	32.100997890562944	34.936349608664116	11.555326606092684
6	19.29049600704421	40.916867017129405	31.409685459811694	8.382951516014693
7	76.63298322488545	6.941089523885105	14.466189132253978	1.9597381189754621
8	77.12929495768725	6.768007509038504	14.101649310045678	2.001048223228561
9	68.29637271775846	11.932644453687452	16.21609365135264	3.554889177201452
10	31.53467863974731	37.50459099571995	22.32228919860051	8.638441165932226
11	31.396175237037095	24.557073723537854	32.0903928380703	11.956358201354753
12	30.98912072777586	23.37755096979544	33.22430678031315	12.40902152211555
13	17.976744938567457	35.550686836578976	31.47378816017928	14.998780064674285
14	13.214887415203865	37.50600815195505	33.48017433856004	15.798930094281042
15	15.463985218115697	28.831854648898364	42.697051441117985	13.007108691867955
16	15.816809882113859	26.87750172361627	39.34394169252386	17.961746701746005
17	13.706215481912245	30.557029791694568	34.14297830971525	21.593776416677947
18	15.19439486365894	32.410646527931796	37.22212813879439	15.17283046961487
19	22.03129978499378	28.559217408536146	32.57905192720257	16.8304308792675
20	21.57070038931644	28.464126225161092	35.838322313762646	14.126851071759832
21	17.016905020535773	33.05072876078427	34.30753376788067	15.624832450799289
22	18.411953618365967	30.71327126661409	32.16972996796518	18.70504514705476
23	18.395372890415324	30.7434094558805	32.87070268038568	17.99051497331849
24	15.09004492621458	30.09335989084118	41.305592972416704	13.511002210527534
25	14.15779231622613	35.389934080977724	36.154300915648264	14.297972687147881
26	14.285052946137908	35.339932085149364	33.393444376972056	16.98157059174067
27	16.69882430356809	33.42093720793295	33.14031665411302	16.739921834385925
28	13.238695639953507	33.55974766116078	37.57029980648732	15.6312568923984
29	13.87251876610096	29.936173645098258	35.965842755650854	20.225464833149932
30	13.881777520170266	36.52691852839662	33.88833895353877	15.702964997894341
31	20.595956711545643	33.70658866638749	31.155801920293936	14.541652701772934
32	19.7873037445283	31.940292373502864	31.59696265627986	16.67544122568898
33	17.546756117568226	35.139050188824264	29.904358487620314	17.4098352059872
34	15.14311742721899	33.20897787370351	32.672938527777795	18.974966171299705
35	21.212655866518997	29.479471429067434	32.098943014022055	17.208929690391518
36	19.586799756532557	35.941160617889565	29.754352500135223	14.717687125442655
37	15.609007539507363	36.671869991976536	33.71556398920978	14.00355847930633
38	15.390056901184765	35.0359520727209	33.41668573922767	16.15730528686667
39	18.750890151260172	33.84539911961531	31.988924451637303	15.414786277487222
40	17.983405572872417	34.142883832632904	31.162368077516554	16.71134251697812
41	14.609794297410547	34.9544419699322	32.191719508879785	18.244044223777472
42	15.31957699775924	35.05010001580129	29.572247923925165	20.058075062514305
43	19.403608693875594	31.906351481672274	30.82085704412859	17.86918278032354
44	16.028131496037513	34.300518844516944	30.480219923752273	19.191129735693277
45	14.922371724331935	38.9806064531154	28.247608371708743	17.84941345084393
46	16.821455556445216	38.99269951965489	29.244152636229458	14.941692287670433
47	19.553803635525366	31.839650661540343	32.11306733783186	16.493478365102433
48	17.07515014179829	31.177696984126197	34.36511754956681	17.382035324508703
49	16.615873425273705	34.39901120285624	32.42531409496506	16.559801276905006
50	15.230792159630369	35.12024924943863	32.52685333920981	17.122105251721194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9022.0
1	15833.5
2	22645.0
3	66013.5
4	109382.0
5	79037.5
6	48693.0
7	48028.5
8	47364.0
9	47688.5
10	48013.0
11	48383.5
12	48754.0
13	45643.0
14	42532.0
15	39266.5
16	36001.0
17	34560.5
18	33120.0
19	34832.5
20	36545.0
21	43926.5
22	51308.0
23	58356.5
24	65405.0
25	82748.0
26	100091.0
27	134378.5
28	168666.0
29	212917.0
30	257168.0
31	257305.5
32	257443.0
33	272836.0
34	288229.0
35	292817.0
36	297405.0
37	295557.0
38	293709.0
39	282503.5
40	271298.0
41	279427.0
42	287556.0
43	279851.5
44	272147.0
45	261901.0
46	251655.0
47	227708.0
48	203761.0
49	187727.0
50	171693.0
51	145685.5
52	119678.0
53	102758.0
54	85838.0
55	75168.5
56	64499.0
57	61546.5
58	58594.0
59	57880.5
60	57167.0
61	50276.5
62	43386.0
63	37057.0
64	30728.0
65	24628.0
66	18528.0
67	16014.0
68	13500.0
69	11101.0
70	8702.0
71	7382.0
72	6062.0
73	4708.5
74	3355.0
75	2699.5
76	2044.0
77	1605.0
78	1166.0
79	836.0
80	506.0
81	358.5
82	211.0
83	153.0
84	95.0
85	78.5
86	62.0
87	39.5
88	17.0
89	37.0
90	57.0
91	39.0
92	21.0
93	15.0
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4233831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.563514800974712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24435632991818	12.794380393623642
2	9.02902168982936	2.991046687956206
3	3.4218987433940864	1.7003601145093412
4	1.799011566589649	1.191918188413294
5	1.1313929114273928	0.9369921617072745
6	0.7520616495341208	0.747407055798232
7	0.547670583348484	0.6349944869345748
8	0.44773959171660094	0.5932913083504234
9	0.3646323784589144	0.5435634417766957
>10	3.5551364148904643	13.084281410534535
>50	0.8902872539335777	10.41457291402337
>100	0.638705038192944	21.74875484692975
>500	0.1242123519376129	14.628711078503844
>1k	0.051851435285584925	12.782918439801854
>5k	0.0012998967063238558	1.3972570132334712
>10k+	7.221648368465866E-4	3.809550457903489
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61696	1.457214518009812	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	34860	0.8233677725917732	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	32520	0.7680986794229623	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	15175	0.35842243112679745	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	14990	0.3540528660685795	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8380	0.19792948750198106	No Hit
TAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	8346	0.19712643230209237	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6897	0.1629021092244825	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	6782	0.16018589310721187	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	6395	0.15104523539083162	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5717	0.1350313699342274	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5519	0.13035475435840493	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	5324	0.1257489965943374	No Hit
AAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5046	0.11918283937171796	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4706	0.1111522873728309	No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4689	0.11075075977288655	No Hit
TAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4517	0.10668824523227308	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4252	0.10042913852725817	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.046411866699450216	0.0
2	0.0	0.0	0.0	0.19332372973791348	0.0
3	0.0	0.0	0.0	0.2867615641720229	0.0
4	0.0	0.0	0.0	0.461709501394836	0.0
5	0.0	0.0	0.0	0.8891710604414772	0.0
6	0.0	0.0	0.0	1.2193448439486603	0.0
7	0.0	0.0	0.0	1.4483809108110361	0.0
8	0.0	0.0	0.0	1.9377721973314477	0.0
9	0.0	0.0	0.0	2.1402365847857414	0.0
10	0.0	0.0	0.0	2.6128345699202447	0.0
11	0.0	0.0	0.0	3.0150943672527317	0.0
12	0.0	0.0	0.0	3.4016237303756336	0.0
13	0.0	0.0	0.0	3.5303959936048463	0.0
14	2.361927058496194E-5	0.0	0.0	3.577516438421845	0.0
15	2.361927058496194E-5	0.0	0.0	3.663230771374672	0.0
16	2.361927058496194E-5	0.0	0.0	3.8231096139642795	0.0
17	2.361927058496194E-5	0.0	0.0	4.018795270760689	0.0
18	2.361927058496194E-5	0.0	0.0	4.273647200372428	0.0
19	2.361927058496194E-5	0.0	0.0	4.419189145716964	0.0
20	2.361927058496194E-5	0.0	0.0	4.582421924729636	0.0
21	2.361927058496194E-5	0.0	0.0	4.793176676159251	0.0
22	2.361927058496194E-5	0.0	0.0	5.014040475399231	0.0
23	2.361927058496194E-5	0.0	0.0	5.256917434824394	0.0
24	2.361927058496194E-5	0.0	0.0	5.441006029763588	0.0
25	2.361927058496194E-5	0.0	0.0	5.602750794729407	0.0
26	2.361927058496194E-5	0.0	0.0	5.76251154096609	0.0
27	2.361927058496194E-5	0.0	0.0	5.921752663249903	0.0
28	4.723854116992388E-5	0.0	0.0	6.096535265578622	0.0
29	4.723854116992388E-5	0.0	0.0	6.278639841788678	0.0
30	4.723854116992388E-5	0.0	0.0	6.522721384013675	0.0
31	4.723854116992388E-5	0.0	0.0	6.750434771723293	0.0
32	4.723854116992388E-5	0.0	0.0	6.957386820588729	0.0
33	4.723854116992388E-5	0.0	0.0	7.152907142491044	0.0
34	4.723854116992388E-5	0.0	0.0	7.356174585145227	0.0
35	7.085781175488582E-5	0.0	0.0	7.614167877744766	0.0
36	7.085781175488582E-5	0.0	0.0	7.842353650866083	0.0
37	7.085781175488582E-5	0.0	0.0	8.065579377164559	0.0
38	7.085781175488582E-5	0.0	0.0	8.267287947960133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	25	4.4448723E-5	44.0	12
CGTATCT	20	7.8588794E-4	44.0	12
ACGCATC	20	7.8588794E-4	44.0	15
TACGACG	70	0.0	44.0	1
TCGACAG	20	7.8588794E-4	44.0	1
CTTACGT	35	1.4473153E-7	44.0	27
GTATACG	160	0.0	42.625	1
CGTAAGT	150	0.0	42.533333	13
CGTTTTT	36885	0.0	41.488953	1
CGTATGG	170	0.0	41.411762	2
GCGCTAA	80	0.0	41.25	7
ACGGGTA	455	0.0	41.098904	5
GTACGCA	70	0.0	40.857143	9
TAGAACG	345	0.0	40.811592	1
CGACGGT	270	0.0	40.74074	28
GTACCGG	240	0.0	40.333332	2
TCACGAC	295	0.0	40.271187	25
TGTTATC	1365	0.0	40.131866	24
TATAGCG	335	0.0	40.059704	1
TACCGGG	775	0.0	40.025803	3
>>END_MODULE