##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463560
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.173054189317455	16.0	16.0	28.0	16.0	31.0
2	21.151130382259037	16.0	16.0	30.0	10.0	31.0
3	22.00998791957891	23.0	16.0	30.0	10.0	31.0
4	26.776997583915783	28.0	19.0	32.0	19.0	35.0
5	30.84527137803089	32.0	28.0	35.0	25.0	35.0
6	31.33898740184658	33.0	30.0	35.0	25.0	35.0
7	29.973304426611442	32.0	28.0	35.0	17.0	35.0
8	30.090074639744586	32.0	28.0	35.0	17.0	35.0
9	23.077228406247304	17.0	17.0	30.0	16.0	35.0
10	25.276721460005177	27.0	17.0	34.0	17.0	37.0
11	27.34800673052032	28.0	17.0	35.0	17.0	39.0
12	27.15811545431012	28.0	17.0	35.0	15.0	39.0
13	27.462270256277506	31.0	17.0	37.0	11.0	39.0
14	30.612680127707307	32.0	27.0	37.0	17.0	40.0
15	27.734386055742515	30.0	17.0	36.0	16.0	40.0
16	29.180824488739322	31.0	25.0	36.0	17.0	39.0
17	27.417956683061522	30.0	17.0	36.0	16.0	39.0
18	27.81642937268099	31.0	17.0	36.0	16.0	39.0
19	26.446822417809994	29.0	18.0	35.0	10.0	37.0
20	26.18727457071361	30.0	18.0	35.0	10.0	37.0
21	28.91695573388558	32.0	25.0	35.0	15.0	38.0
22	30.689714384329967	33.0	27.0	36.0	16.0	38.0
23	30.556622659418412	33.0	27.0	36.0	17.0	39.0
24	31.96792863922685	35.0	30.0	37.0	19.0	39.0
25	31.81712615411166	34.0	30.0	37.0	21.0	39.0
26	25.308009750625594	32.0	15.0	36.0	0.0	38.0
27	27.7646906549314	32.0	18.0	36.0	10.0	39.0
28	28.801026835792563	33.0	23.0	36.0	12.0	38.0
29	29.682929933557684	33.0	25.0	36.0	14.0	38.0
30	29.715689446889293	33.0	26.0	36.0	11.0	38.0
31	28.54274743291052	32.0	24.0	35.0	11.0	38.0
32	28.576991112261627	33.0	24.0	36.0	9.0	38.0
33	28.716327983432567	33.0	24.0	36.0	9.0	38.0
34	27.719520666148934	33.0	21.0	36.0	8.0	38.0
35	26.909105617395806	32.0	18.0	35.0	7.0	38.0
36	27.19357149020623	32.0	21.0	35.0	8.0	38.0
37	26.854592717231856	32.0	18.0	35.0	8.0	38.0
38	26.83525541461731	31.0	19.0	35.0	8.0	38.0
39	26.743953317801363	31.0	19.0	35.0	8.0	38.0
40	26.60851885408577	31.0	18.0	35.0	8.0	38.0
41	25.00055224782121	29.0	15.0	34.0	7.0	37.0
42	24.868716023815686	29.0	15.0	34.0	7.0	37.0
43	25.203190525498318	29.0	15.0	35.0	7.0	37.0
44	26.251604970230392	31.0	18.0	35.0	7.0	37.0
45	25.959554318750538	31.0	18.0	35.0	7.0	37.0
46	24.39362542065752	28.0	15.0	34.0	7.0	36.0
47	24.88291051859522	29.0	15.0	34.0	7.0	36.0
48	25.512919578911035	30.0	18.0	34.0	7.0	37.0
49	24.7768185348175	29.0	15.0	34.0	7.0	37.0
50	25.802955388730695	30.0	15.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	51.0
13	482.0
14	1903.0
15	4631.0
16	7482.0
17	10124.0
18	11490.0
19	12045.0
20	13318.0
21	14398.0
22	16393.0
23	19614.0
24	23890.0
25	30043.0
26	35589.0
27	39355.0
28	39825.0
29	36107.0
30	33668.0
31	30844.0
32	26842.0
33	22205.0
34	16688.0
35	11305.0
36	4400.0
37	840.0
38	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	60.27633963241005	10.643498144792476	8.172836310294244	20.907325912503236
2	25.43726809905945	58.99063767365605	8.760031063939945	6.8120631633445505
3	10.598628009319182	57.23983950297696	26.03460177754767	6.1269307101561825
4	11.208257830701527	11.54478384675123	68.64440417637415	8.602554146173096
5	44.330615238588315	12.93187505393045	28.39438260419363	14.3431271032876
6	9.885235999654846	51.560100094917594	31.93782897575287	6.616834929674692
7	28.6122616274053	5.406635602726723	61.80753300543619	4.173569764431789
8	24.684830442661145	37.88225903874364	28.729830011217533	8.703080507377686
9	18.771680041418588	4.220812839761843	16.21667098110277	60.790836137716795
10	39.5154888256105	11.702476486323238	33.26063508499439	15.521399603071877
11	38.306799551298646	12.580679955129865	32.56450081974286	16.54801967382863
12	15.79018897230132	10.892872551557511	39.80261454827854	33.51432392786263
13	26.20631633445509	19.70359823971007	45.75934075416343	8.330744671671413
14	11.632151177841056	42.31879368366555	29.83346276641643	16.21559237207697
15	4.025153162481663	13.81504012425576	37.290102683579256	44.86970402968332
16	4.284019328673742	14.89839502976961	26.937613253947706	53.879972387608944
17	5.5356372422124425	27.23121063077056	38.70113901113125	28.53201311588575
18	6.099749762706015	22.73103805332643	33.53870049184572	37.630511692121836
19	7.734489602208991	23.48779014582794	30.36823712140823	38.40948313055484
20	14.302787125722668	22.96660626456122	42.87276727931659	19.857839330399514
21	12.707524376563983	35.192855293813096	33.70480628181897	18.39481404780395
22	7.555009060315816	17.051082923461905	46.33876952282336	29.055138493398914
23	19.03507636551903	29.063767365605315	37.081931141599796	14.819225127275864
24	17.523729398567607	27.13996030718785	31.31568728967124	24.020623004573302
25	8.650444386918629	44.59444300629908	28.835965139356283	17.919147467426008
26	9.572007938562429	31.58016222279748	35.38765208387264	23.460177754767454
27	21.526231771507465	27.665890068168093	29.419924065924587	21.38795409439986
28	7.429243247907498	30.551600655794285	42.24695832254724	19.77219777375097
29	17.19691086375011	28.226982483389424	37.01225299853309	17.56385365432738
30	7.9590560013806195	43.50893088273363	36.7960997497627	11.735913366123048
31	20.09621192510139	17.643670722236603	32.03576667529554	30.22435067736647
32	28.306368107688325	31.62848390715334	25.415480196738287	14.649667788420054
33	18.617007507118817	24.416688238847183	25.21291742169299	31.753386832341015
34	17.873414444732074	26.05293813098628	25.812839761843126	30.26080766243852
35	17.26874622486841	33.20562602467857	25.771636897057554	23.75399085339546
36	22.690482353956337	28.278108551212355	29.200750711881955	19.83065838294935
37	16.392268530503063	27.070066442315987	33.96475105703685	22.5729139701441
38	15.965570799896453	28.898524462852706	26.039994822676675	29.095909914574165
39	24.87013547329364	21.22141686081629	26.762878591768057	27.14556907412201
40	11.139226853050307	34.010699801535935	31.166623522305635	23.68344982310812
41	17.622529985330917	22.680559150918974	25.077228406247304	34.619682457502805
42	14.70553973595651	24.44235913366123	35.9890844766589	24.863016653723356
43	27.548537406161017	21.916472517042024	21.922728449391666	28.6122616274053
44	15.673052032099404	20.438346708085252	31.80861161446199	32.07998964535335
45	15.840883596513935	19.20247648632324	29.5349037880749	35.421736129087925
46	31.93675036672707	21.943869186297352	23.78332901889723	22.33605142807835
47	11.242341875916818	21.862326343946847	42.966821986366384	23.928509793769955
48	17.554793338510656	22.221071705928036	25.513849339891276	34.71028561567003
49	13.678488221589438	18.069505565622574	40.572094227284495	27.679911985503498
50	19.532099404607816	17.105229096557082	33.61463456726206	29.74803693157304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5961.0
1	5189.5
2	4418.0
3	11740.0
4	19062.0
5	14030.5
6	8999.0
7	7809.5
8	6620.0
9	6754.5
10	6889.0
11	6748.5
12	6608.0
13	6354.5
14	6101.0
15	5811.0
16	5521.0
17	5264.5
18	5008.0
19	4538.5
20	4069.0
21	3802.5
22	3536.0
23	3129.0
24	2722.0
25	2630.0
26	2538.0
27	2443.0
28	2348.0
29	2351.0
30	2354.0
31	2349.5
32	2345.0
33	2523.0
34	2701.0
35	2973.5
36	3246.0
37	3409.0
38	3572.0
39	4812.5
40	6053.0
41	12111.0
42	18169.0
43	33354.5
44	48540.0
45	57818.5
46	67097.0
47	59344.0
48	51591.0
49	48173.0
50	44755.0
51	38175.5
52	31596.0
53	25411.5
54	19227.0
55	16663.0
56	14099.0
57	13156.5
58	12214.0
59	11286.0
60	10358.0
61	9498.0
62	8638.0
63	7831.0
64	7024.0
65	6144.0
66	5264.0
67	4562.5
68	3861.0
69	3328.5
70	2796.0
71	2482.0
72	2168.0
73	1906.5
74	1645.0
75	1430.0
76	1215.0
77	1082.0
78	949.0
79	807.0
80	665.0
81	562.5
82	460.0
83	383.5
84	307.0
85	228.0
86	149.0
87	111.0
88	73.0
89	45.0
90	17.0
91	12.0
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	463560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.51548445408894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.65399391940727	37.266520158193664
2	5.600946797403326	4.762539329863608
3	2.0292511667768887	2.5882378930363394
4	1.0526167768641894	1.7901004885153065
5	0.6881442428562965	1.462839292966384
6	0.48066265036250544	1.2261363263488974
7	0.3426049640800049	1.0196211216966007
8	0.28852345069771373	0.9813371426222998
9	0.22743256420869204	0.870246508317141
>10	1.4442832224429065	11.186368227864513
>50	0.09715809523923646	2.8955191442295103
>100	0.06995382857225024	5.530920729402941
>500	0.009438214966097256	2.776790349124966
>1k	0.010548593197402815	9.501372145811565
>5k	0.003331134693916679	9.949850695908253
>10k+	0.0011103782313055595	6.191600446098009
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGCCTCTTATACACATCTGACGCAATGACAGTCGTATG	14589	3.1471654154801962	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11642	2.5114332556734835	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	8547	1.8437742687030805	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT	7894	1.7029079299335577	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCAATGACAGTCGTATGCC	7465	1.6103632755198896	No Hit
GAATCTTTCTCTTACCCACATCTGACGCAATGACAGTCGTATGCCGTCTT	7051	1.5210544481836223	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG	6077	1.3109414099577186	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5119	1.1042799206143756	No Hit
GAACTGTCCCTTATCCACATCTGACGCAATGACAGTCGTATGCCGTCTTC	4991	1.0766675295538872	No Hit
GAATCTGTCTCTTACCCACATCTGACGCAATGACAGTCGTATGCCGTCTT	3137	0.6767193027871257	No Hit
GAACTGTCCCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	2801	0.6042367762533437	No Hit
GCTGTCTCCTATACCCATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	2554	0.5509534903788075	No Hit
GAATGACTCTCTCTCCTACACATCTGACGCAATGACAGTCGTATGCCGTC	2541	0.5481491069117267	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	2485	0.5360686858227629	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCT	2290	0.4940029338165502	No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCAATGACAGTCGTATGCC	2278	0.4914142721546294	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAG	2215	0.47782379842954525	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	1985	0.4282077832427302	No Hit
GAATCTTTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTT	1845	0.39800673052032104	No Hit
GAATGATCCGTCTCCCATACACATCTGACGCAATGACAGTCGTATGCCGT	1782	0.3844162567952369	No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCAATGACAGTCGT	1694	0.3654327379411511	No Hit
GCTGTCTCCTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1608	0.34688066269738543	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1493	0.3220726551039779	No Hit
GAATGCTGCCTCTTCCACACATCTGACGCAATGACAGTCGTATGCCGTCT	1277	0.27547674518940374	No Hit
GAATCTTTCTCTTACCCCCATCTGACGCAATGACAGTCGTATGCCGTCTT	1159	0.250021572180516	No Hit
GAACTGTCCCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCCTC	1060	0.2286651134696695	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCG	1058	0.22823366985934937	No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	950	0.2049357149020623	No Hit
GAATGATACGGCGCCCTCTCTTATACACATCTGACGCAATGACAGTCGTA	928	0.20018983518854086	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.19220812839761844	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCAATGACAG	843	0.1818534817499353	No Hit
GAATGCTGCCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	837	0.18055915091897487	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	772	0.16653723358357062	No Hit
GAATGATCCGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGT	726	0.15661403054620762	No Hit
GAATCTGTCTCTTACACACATCTGACGCAATGACAGTCGTATGCCGTCTT	669	0.1443178876520839	No Hit
GAATGATACGGCTGCCTCTTATACAAATCTGACGCAATGACAGTCGTATG	627	0.1352575718353611	No Hit
GAATGACTCGCTCTCCTACACATCTGACGCAATGACAGTCGTATGCCGTC	616	0.1328846319786004	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	605	0.13051169212183966	No Hit
CGCTGTCTCTTATACCCATCTGACGCAATGACAGTCGTATGCCGTCTTCT	593	0.12792303045991887	No Hit
CGTTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.12641297782379843	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAATGACAGTCGTATG	567	0.12231426352575718	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	520	0.1121753386832341	No Hit
GAATCTGTCTCTTATCCACATCTGACGCAATGACAGTCGTATGCCGTCTT	517	0.1115281732677539	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGT	517	0.1115281732677539	No Hit
GAATGATACGGCGACCGCCTCTTATACACATCTGACGCAATGACAGTCGT	472	0.10182069203555094	No Hit
GAACTGTCCCTTATCCACCTCTGACGCAATGACAGTCGTATGCCGTCTTC	469	0.10117352662007074	No Hit
AAAAAAAACAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	469	0.10117352662007074	No Hit
GAATCTTTCTCTTACACACATCTGACGCAATGACAGTCGTATGCCGTCTT	468	0.1009578048149107	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09729053412718958	0.0
2	0.0	0.0	0.0	0.7213737164552593	0.0
3	0.0	0.0	0.0	1.3607731469496938	0.0
4	0.0	0.0	0.0	1.7589955992751747	0.0
5	0.0	0.0	0.0	5.905815859867116	0.0
6	0.0	0.0	0.0	6.050133747519199	0.0
7	0.0	0.0	0.0	6.193157304340323	0.0
8	0.0	0.0	0.0	6.387091207179222	0.0
9	0.0	0.0	0.0	6.568513245318837	0.0
10	0.0	0.0	0.0	9.675338683234102	0.0
11	0.0	0.0	0.0	9.829148330313227	0.0
12	0.0	0.0	0.0	12.273492104581932	0.0
13	0.0	0.0	0.0	12.39817930796445	0.0
14	0.0	0.0	0.0	12.487272413495557	0.0
15	0.0	0.0	0.0	12.751100181206317	0.0
16	0.0	0.0	0.0	13.172620588489085	0.0
17	0.0	0.0	0.0	13.294503408404521	0.0
18	0.0	0.0	0.0	13.392009664336872	0.0
19	0.0	0.0	0.0	14.386271464319613	0.0
20	0.0	0.0	0.0	14.54115972042454	0.0
21	0.0	0.0	0.0	14.595521615324877	0.0
22	0.0	0.0	0.0	14.772197773750971	0.0
23	0.0	0.0	0.0	14.846406074726033	0.0
24	0.0	0.0	0.0	15.076149797221504	0.0
25	0.0	0.0	0.0	15.142592113210803	0.0
26	0.0	0.0	0.0	15.478255242039866	0.0
27	0.0	0.0	0.0	15.88855811545431	0.0
28	0.0	0.0	0.0	15.980239882647338	0.0
29	0.0	0.0	0.0	16.099102597290535	0.0
30	0.0	0.0	0.0	16.15109155233411	0.0
31	0.0	0.0	0.0	16.246224868409698	0.0
32	0.0	0.0	0.0	16.360988868754855	0.0
33	0.0	0.0	0.0	16.406074726033307	0.0
34	0.0	0.0	0.0	16.500776598498575	0.0
35	0.0	0.0	0.0	16.55190266632151	0.0
36	0.0	0.0	0.0	16.596772801794806	0.0
37	0.0	0.0	0.0	16.63991716282682	0.0
38	0.0	0.0	0.0	16.67853136595047	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAT	25	4.438718E-5	44.0	20
CATGGTA	20	7.851615E-4	44.0	44
CGGAATT	70	0.0	44.0	9
GTGCAAG	40	8.294592E-9	44.0	1
TTAGGCG	35	1.4435864E-7	44.0	4
CGGAAAT	20	7.851615E-4	44.0	21
ACCGGTG	150	0.0	44.0	17
ATTAGTC	25	4.438718E-5	44.0	44
GTACATG	20	7.851615E-4	44.0	41
TGCTTAG	20	7.851615E-4	44.0	1
TTAGCTG	45	4.783942E-10	44.0	5
CGACGGG	20	7.851615E-4	44.0	3
CACATAG	20	7.851615E-4	44.0	34
CGTGAAA	25	4.438718E-5	44.0	20
TGGGTAC	35	1.4435864E-7	44.0	6
CGTTGAA	30	2.5244353E-6	44.0	19
TAGCGGG	70	0.0	44.0	3
TGAACGG	35	1.4435864E-7	44.0	2
GTGATCC	25	4.438718E-5	44.0	3
GTTAGCG	20	7.851615E-4	44.0	1
>>END_MODULE