FastQCFastQC Report
Sat 18 Jun 2016
SRR3549154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214791
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328082.7007114804110337No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT44830.3690346734541168No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT32670.2689351501616327No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30830.25378851176869105No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17670.1454571197843909No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14400.11853890916215218No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT13830.11384674400781698No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC13480.11096558996568134No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG207.856658E-444.0000041
TCGGATC207.856658E-444.00000444
TACGCGG408.312782E-944.0000042
TCCGTAG207.856658E-444.0000041
ACGTAGC207.856658E-444.00000444
ATCGGTT207.856658E-444.00000439
TACGTAA207.856658E-444.00000427
CTATGCG302.5278368E-644.01
CGTATAA254.4429915E-544.027
GTCGACG254.4429915E-544.01
CGCATCG302.5278368E-644.021
TTATGCA254.4429915E-544.043
GAATCCC254.4429915E-544.09
GCATTAC302.5278368E-644.044
CTACGAA850.044.011
ACTACCG302.5278368E-644.01
TCCGTTA254.4429915E-544.024
ACATCGG254.4429915E-544.042
TAGTGCG254.4429915E-544.01
CGCAAGT254.4429915E-544.020
Produced by FastQC (version 0.11.5)