##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1214791
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.137514189683657	31.0	28.0	33.0	25.0	33.0
2	28.93585563277963	31.0	28.0	33.0	16.0	33.0
3	28.370787238298604	31.0	28.0	33.0	16.0	34.0
4	32.00460984646742	35.0	32.0	35.0	25.0	37.0
5	33.74501375133665	35.0	33.0	35.0	30.0	37.0
6	33.571502423050546	35.0	33.0	37.0	28.0	37.0
7	34.091052699600176	35.0	33.0	37.0	30.0	37.0
8	34.6141262159499	35.0	35.0	37.0	32.0	37.0
9	36.476782425948166	39.0	37.0	39.0	32.0	39.0
10	36.36069167453496	39.0	35.0	39.0	32.0	39.0
11	36.451129453543864	39.0	35.0	39.0	32.0	39.0
12	35.30192436394409	37.0	34.0	39.0	30.0	39.0
13	35.24100606606404	37.0	34.0	39.0	27.0	39.0
14	36.09630710138617	38.0	34.0	40.0	29.0	41.0
15	36.67649249953284	38.0	35.0	40.0	31.0	41.0
16	36.33281198164952	38.0	34.0	40.0	31.0	41.0
17	35.75750396570274	37.0	34.0	40.0	30.0	41.0
18	35.44877596228487	37.0	34.0	39.0	29.0	40.0
19	34.848187877585524	37.0	34.0	39.0	27.0	40.0
20	34.94127796468693	35.0	34.0	39.0	29.0	40.0
21	35.187920391244255	35.0	34.0	39.0	30.0	40.0
22	35.468033595902504	35.0	34.0	39.0	31.0	40.0
23	35.82252420375192	35.0	35.0	40.0	31.0	41.0
24	35.515011224152964	35.0	34.0	39.0	31.0	41.0
25	35.20387951507708	35.0	34.0	39.0	30.0	41.0
26	35.2221279215931	35.0	34.0	39.0	30.0	41.0
27	35.49982424960343	35.0	34.0	40.0	31.0	41.0
28	35.431166348779335	35.0	35.0	40.0	31.0	41.0
29	35.51378302934414	36.0	35.0	40.0	31.0	41.0
30	35.11715101610071	36.0	34.0	40.0	30.0	41.0
31	34.37743694182785	35.0	34.0	39.0	26.0	41.0
32	34.11997619343574	35.0	34.0	39.0	24.0	41.0
33	33.55708759778431	35.0	33.0	39.0	21.0	41.0
34	32.98862026472043	35.0	33.0	40.0	16.0	41.0
35	32.53858153377824	35.0	33.0	40.0	12.0	41.0
36	32.06228890401724	35.0	32.0	40.0	10.0	41.0
37	31.945418594638912	35.0	31.0	40.0	10.0	41.0
38	31.789196660166233	35.0	31.0	39.0	10.0	40.0
39	31.64794520209649	35.0	31.0	39.0	10.0	40.0
40	31.4440607478982	35.0	30.0	39.0	10.0	40.0
41	31.173376325639556	35.0	30.0	39.0	8.0	40.0
42	31.309117370806995	35.0	30.0	39.0	8.0	40.0
43	31.072550751528453	35.0	30.0	39.0	8.0	40.0
44	30.855263991913013	35.0	29.0	39.0	8.0	40.0
45	30.715957724415148	35.0	29.0	38.0	8.0	40.0
46	30.523790512112782	35.0	28.0	38.0	8.0	40.0
47	30.4997822670731	35.0	29.0	38.0	8.0	40.0
48	30.497443593177756	35.0	29.0	38.0	7.0	40.0
49	30.501882216776384	35.0	29.0	38.0	7.0	40.0
50	30.602534921644956	35.0	28.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	9.0
13	14.0
14	54.0
15	151.0
16	366.0
17	888.0
18	1866.0
19	3095.0
20	4942.0
21	7131.0
22	10383.0
23	14803.0
24	21981.0
25	33381.0
26	46322.0
27	49801.0
28	44002.0
29	39551.0
30	40654.0
31	47489.0
32	62150.0
33	95037.0
34	155069.0
35	108216.0
36	100165.0
37	136884.0
38	155912.0
39	34468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.798429524090977	20.56608914619881	24.84336811846647	28.79211321124375
2	44.25609014225492	19.46079613694866	24.860984317466954	11.422129403329462
3	26.20022703493852	17.70493854498428	44.00114916886938	12.093685251207821
4	24.44527494853024	22.07943588650229	40.49198586423508	12.983303300732391
5	17.566560832274853	26.359678331498998	41.491170086047724	14.582590750178426
6	16.804783703534188	34.84492394164922	38.980203178983054	9.370089175833538
7	68.72466127918301	5.122691886917173	23.65024107027464	2.502405763625183
8	69.89943126019207	3.964550280665563	23.02519528050504	3.110823178637313
9	66.9632060165082	5.012302527759919	24.060599724561673	3.963891731170218
10	40.3778098454796	21.903520852558174	28.145170650753915	9.573498651208315
11	36.12934241363329	17.77803753896761	32.87742500561825	13.21519504178085
12	32.360628289146035	17.742722822279717	38.517160565068394	11.379488323505853
13	17.333269673548784	32.88376354451095	38.27901260381415	11.503954178126113
14	12.524541258537477	37.10860551321174	38.740491162677365	11.62636206557342
15	11.543961059968339	21.951677284405303	54.719783073796236	11.784578581830125
16	12.760713571305683	15.438705094127304	55.618044585447215	16.18253674911981
17	12.366489379654608	15.883884552980717	39.875748173965725	31.873877893398944
18	16.62788084534706	21.942539910157386	44.247282042754684	17.182297201740877
19	22.3546272568697	23.223418678603974	37.20598851983592	17.215965544690405
20	24.203258008990847	19.98854123878099	36.61905628210943	19.189144470118727
21	20.58724504873678	22.790998616222875	39.869409635073026	16.75234669996732
22	17.65126676111364	20.54773207901606	36.33332811981649	25.4676730400538
23	15.067200860065642	26.768061337299997	36.78657480998789	21.37816299264647
24	16.124831349590178	22.511032762014207	47.135515491965286	14.228620396430333
25	17.625501012108256	19.862264373048532	44.40903826254887	18.103196352294347
26	13.304181542339382	27.7222172373684	38.198669565382026	20.774931654910187
27	14.003972699830673	27.936904372850968	41.907291048419026	16.151831878899333
28	12.352412884191603	24.949229949843225	48.4482515922492	14.250105573715974
29	13.710671218341261	22.968560023905347	45.42435694699747	17.896411810755925
30	15.593546544220363	24.66638294159242	44.61343556216666	15.126634952020554
31	25.908489608500556	22.771242131362516	35.52668730670543	15.793580953431496
32	24.028577755350508	24.55237156021077	36.85786279285902	14.561187891579705
33	22.63401688026994	22.465016616026954	34.517542523775695	20.38342397992741
34	16.430233678056556	24.53170956979431	35.0456169003557	23.99243985179344
35	17.41394198672858	22.082234721857503	37.496902759404705	23.006920532009207
36	28.51247663178275	22.01572122282763	32.45257826243362	17.019223882956
37	17.650031980809867	27.987118771871046	34.48733156567673	19.875517681642357
38	18.96564923513592	30.712690495731366	29.93247398112103	20.389186288011683
39	18.083110592686314	26.509745297750808	32.35865264066	23.04849146890288
40	20.31896844807049	22.232960237604658	35.221614252986726	22.226457061338124
41	16.205915256204566	21.891749280328877	35.883538814495665	26.018796648970895
42	20.103046532284154	25.02356372413032	29.805044653771716	25.068345089813803
43	19.752451244699706	23.254205867511367	30.70363544017037	26.28970744761856
44	17.631263320192527	26.21907801424278	33.82120875113497	22.328449914429722
45	19.183958392842886	31.49603512044459	28.450079067098784	20.86992741961374
46	22.71592397375351	27.80840490257172	30.17770134945024	19.297969774224537
47	21.8655719378889	24.863289240700663	30.402842958171405	22.868295863239027
48	19.471332928874187	25.61732841287102	33.1151613734379	21.796177284816896
49	19.68313891031461	23.61657272732511	34.8734062073229	21.82688215503737
50	18.495362576772465	26.92372597426224	33.44443612111054	21.13647532785475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	995.0
1	1377.5
2	1760.0
3	23689.5
4	45619.0
5	32246.5
6	18874.0
7	19642.5
8	20411.0
9	22193.5
10	23976.0
11	23968.5
12	23961.0
13	23172.5
14	22384.0
15	20984.5
16	19585.0
17	18185.5
18	16786.0
19	15549.0
20	14312.0
21	13281.0
22	12250.0
23	11143.0
24	10036.0
25	9436.0
26	8836.0
27	9576.0
28	10316.0
29	11186.0
30	12056.0
31	13708.5
32	15361.0
33	16663.5
34	17966.0
35	20316.5
36	22667.0
37	25945.5
38	29224.0
39	36540.5
40	43857.0
41	64827.5
42	85798.0
43	99095.5
44	112393.0
45	116542.0
46	120691.0
47	116100.5
48	111510.0
49	109486.0
50	107462.0
51	93152.0
52	78842.0
53	65531.5
54	52221.0
55	45782.0
56	39343.0
57	35866.0
58	32389.0
59	28755.0
60	25121.0
61	21853.0
62	18585.0
63	16484.5
64	14384.0
65	11426.5
66	8469.0
67	6967.5
68	5466.0
69	4671.5
70	3877.0
71	3529.0
72	3181.0
73	2512.0
74	1843.0
75	1339.0
76	835.0
77	710.5
78	586.0
79	419.5
80	253.0
81	187.0
82	121.0
83	86.5
84	52.0
85	56.5
86	61.0
87	49.0
88	37.0
89	27.0
90	17.0
91	12.0
92	7.0
93	7.0
94	7.0
95	5.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1214791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.595623914142564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2081381337198	22.936017529254478
2	7.317796438289971	4.18513909659184
3	2.7874785554443595	2.3912906532067293
4	1.5812900014573024	1.8087189672346788
5	1.1079181738759327	1.5840805713899893
6	0.84255951551657	1.4456109018596404
7	0.6653088222593768	1.3317444607663182
8	0.5277863353879829	1.2073903643022617
9	0.43384223024089485	1.116539032863733
>10	3.266360877136866	19.795771637420263
>50	0.7840796245346369	16.049079391013173
>100	0.4661699783390077	20.09430859373889
>500	0.006936193106057944	1.3146126032282284
>1k	0.004046112645200467	2.028323198656592
>5k	0.0	0.0
>10k+	2.890080460857476E-4	2.711372998473107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32808	2.7007114804110337	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	4483	0.3690346734541168	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3267	0.2689351501616327	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3083	0.25378851176869105	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1767	0.1454571197843909	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1440	0.11853890916215218	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	1383	0.11384674400781698	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	1348	0.11096558996568134	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6463737383632247E-4	0.0	0.0	0.059022498520321605	0.0
2	1.6463737383632247E-4	0.0	0.0	0.26169110571283455	0.0
3	1.6463737383632247E-4	0.0	0.0	0.3570984638509834	0.0
4	1.6463737383632247E-4	0.0	0.0	0.5666818407446219	0.0
5	1.6463737383632247E-4	0.0	0.0	1.0498102142673102	0.0
6	1.6463737383632247E-4	0.0	0.0	1.3945608750805694	0.0
7	1.6463737383632247E-4	0.0	0.0	1.6243123302691573	0.0
8	1.6463737383632247E-4	0.0	0.0	2.124069078549314	0.0
9	2.469560607544837E-4	0.0	0.0	2.3217985645267376	0.0
10	2.469560607544837E-4	0.0	0.0	2.7550418137770203	0.0
11	2.469560607544837E-4	0.0	0.0	3.1073657937867503	0.0
12	2.469560607544837E-4	0.0	0.0	3.4756595990586034	0.0
13	4.1159343459080613E-4	0.0	0.0	3.6326413350115367	0.0
14	4.1159343459080613E-4	0.0	0.0	3.692404701714122	0.0
15	4.1159343459080613E-4	0.0	0.0	3.7706897729732933	0.0
16	4.1159343459080613E-4	0.0	0.0	3.9259428165009456	0.0
17	4.1159343459080613E-4	0.0	0.0	4.124166214599878	0.0
18	4.1159343459080613E-4	0.0	0.0	4.45623979762774	0.0
19	4.1159343459080613E-4	0.0	0.0	4.575272618911401	0.0
20	4.1159343459080613E-4	0.0	0.0	4.699656154844742	0.0
21	4.939121215089674E-4	0.0	0.0	4.852933549886359	0.0
22	4.939121215089674E-4	0.0	0.0	5.008845142909356	0.0
23	5.762308084271286E-4	0.0	0.0	5.187559012208684	0.0
24	6.585494953452899E-4	0.0	0.0	5.330875846133203	0.0
25	6.585494953452899E-4	0.0	0.0	5.431057688112605	0.0
26	6.585494953452899E-4	0.0	0.0	5.533462134638798	0.0
27	6.585494953452899E-4	0.0	0.0	5.6345494821743	0.0
28	6.585494953452899E-4	0.0	0.0	5.767000249425621	0.0
29	6.585494953452899E-4	0.0	0.0	5.89014900505519	0.0
30	6.585494953452899E-4	0.0	0.0	6.0481185652511416	0.0
31	6.585494953452899E-4	0.0	0.0	6.194069597157042	0.0
32	6.585494953452899E-4	0.0	0.0	6.318453133090383	0.0
33	6.585494953452899E-4	0.0	0.0	6.466709088229992	0.0
34	6.585494953452899E-4	0.0	0.0	6.610355196902183	0.0
35	8.231868691816123E-4	0.0	0.0	6.831051596529774	0.0
36	9.055055560997736E-4	0.0	0.0	6.974368430454292	0.0
37	9.055055560997736E-4	0.0	0.0	7.105255142654168	0.0
38	9.878242430179348E-4	0.0	0.0	7.246843284153406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAACG	20	7.856658E-4	44.000004	1
TCGGATC	20	7.856658E-4	44.000004	44
TACGCGG	40	8.312782E-9	44.000004	2
TCCGTAG	20	7.856658E-4	44.000004	1
ACGTAGC	20	7.856658E-4	44.000004	44
ATCGGTT	20	7.856658E-4	44.000004	39
TACGTAA	20	7.856658E-4	44.000004	27
CTATGCG	30	2.5278368E-6	44.0	1
CGTATAA	25	4.4429915E-5	44.0	27
GTCGACG	25	4.4429915E-5	44.0	1
CGCATCG	30	2.5278368E-6	44.0	21
TTATGCA	25	4.4429915E-5	44.0	43
GAATCCC	25	4.4429915E-5	44.0	9
GCATTAC	30	2.5278368E-6	44.0	44
CTACGAA	85	0.0	44.0	11
ACTACCG	30	2.5278368E-6	44.0	1
TCCGTTA	25	4.4429915E-5	44.0	24
ACATCGG	25	4.4429915E-5	44.0	42
TAGTGCG	25	4.4429915E-5	44.0	1
CGCAAGT	25	4.4429915E-5	44.0	20
>>END_MODULE