FastQCFastQC Report
Sat 18 Jun 2016
SRR3549153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211818
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331492.735476779516396No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT46820.38636164836633885No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT32360.2670367992553337No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30050.24797453082888687No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18110.14944488363764194No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT14690.12122282388939594No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14460.1193248491110051No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC13520.11156790871236441No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12450.10273819996072017No Hit
TAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12130.10009753939948078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGG650.044.0000042
TCGCACC254.4429842E-544.011
GGTAATC502.7284841E-1144.08
CGTATAA207.8566506E-444.027
GATCGGC302.5278314E-644.034
ACGTTCC207.8566506E-444.011
CGTTCGA254.4429842E-544.014
TAGCCGT700.044.044
ACGATCG207.8566506E-444.032
CTAACGT207.8566506E-444.01
TACGCGG351.4461693E-744.02
ACGGATC207.8566506E-444.05
ACAATCG207.8566506E-444.02
ACTACCG302.5278314E-644.01
CGAGAGT408.312782E-944.038
TCGACTG254.4429842E-544.01
TCGACAG351.4461693E-744.01
TATCGTG302.5278314E-644.01
TGTCGTG207.8566506E-444.014
TAATGCG207.8566506E-444.01
Produced by FastQC (version 0.11.5)