##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549153_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211818
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.113054105484487	31.0	28.0	33.0	25.0	33.0
2	28.916116941652955	31.0	28.0	33.0	16.0	33.0
3	28.36684881723163	31.0	28.0	33.0	16.0	34.0
4	31.98920299913023	35.0	32.0	35.0	25.0	37.0
5	33.767510467743506	35.0	33.0	35.0	30.0	37.0
6	33.65531622735427	35.0	33.0	37.0	28.0	37.0
7	34.156074592059205	35.0	33.0	37.0	30.0	37.0
8	34.65353955792041	35.0	35.0	37.0	32.0	37.0
9	36.593402639670316	39.0	37.0	39.0	32.0	39.0
10	36.37284724273777	39.0	35.0	39.0	32.0	39.0
11	36.49858229536118	39.0	35.0	39.0	32.0	39.0
12	35.26185945414245	37.0	34.0	39.0	30.0	39.0
13	35.25671676769944	37.0	34.0	39.0	27.0	39.0
14	36.11072289733277	38.0	34.0	40.0	29.0	41.0
15	36.7170532208632	38.0	35.0	40.0	31.0	41.0
16	36.37079825518354	38.0	34.0	40.0	31.0	41.0
17	35.808942431949355	37.0	34.0	40.0	30.0	41.0
18	35.517585148925	37.0	34.0	39.0	29.0	40.0
19	34.92328963590242	37.0	34.0	39.0	28.0	40.0
20	34.92555482754011	35.0	34.0	39.0	29.0	40.0
21	35.113675485922805	35.0	34.0	39.0	30.0	40.0
22	35.44344365242966	35.0	34.0	39.0	31.0	40.0
23	35.84090845324958	35.0	35.0	40.0	31.0	41.0
24	35.451921823244085	35.0	34.0	39.0	31.0	41.0
25	35.14576198736114	35.0	34.0	39.0	30.0	41.0
26	35.195310681967094	35.0	34.0	39.0	30.0	41.0
27	35.529896403585354	35.0	34.0	40.0	31.0	41.0
28	35.45918694061319	35.0	35.0	40.0	31.0	41.0
29	35.538251618642406	36.0	35.0	40.0	31.0	41.0
30	35.17183438437125	36.0	34.0	40.0	30.0	41.0
31	34.42552016887024	35.0	34.0	39.0	27.0	41.0
32	34.20626694767696	35.0	34.0	39.0	24.0	41.0
33	33.65997781845129	35.0	33.0	40.0	21.0	41.0
34	33.025176222832144	35.0	33.0	40.0	16.0	41.0
35	32.58652619452756	35.0	33.0	40.0	12.0	41.0
36	32.107880886403734	35.0	32.0	40.0	10.0	41.0
37	31.979545608333925	35.0	31.0	40.0	10.0	41.0
38	31.855923084159503	35.0	31.0	39.0	10.0	40.0
39	31.674352914381533	35.0	31.0	39.0	10.0	40.0
40	31.437916419792412	35.0	30.0	39.0	10.0	40.0
41	31.170484346659318	35.0	30.0	39.0	8.0	40.0
42	31.31020417257377	35.0	30.0	39.0	8.0	40.0
43	31.094118093641125	35.0	30.0	39.0	8.0	40.0
44	30.849416331495323	35.0	29.0	39.0	8.0	40.0
45	30.70822021128585	35.0	29.0	38.0	8.0	40.0
46	30.49999834958715	35.0	28.0	38.0	7.0	40.0
47	30.487253861553466	35.0	29.0	38.0	8.0	40.0
48	30.493245685408205	35.0	29.0	38.0	7.0	40.0
49	30.549350645063864	35.0	29.0	38.0	7.0	40.0
50	30.66857481899097	35.0	29.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	7.0
13	20.0
14	45.0
15	140.0
16	372.0
17	872.0
18	1741.0
19	2993.0
20	4585.0
21	7011.0
22	9935.0
23	14550.0
24	21626.0
25	33104.0
26	46421.0
27	49647.0
28	43909.0
29	39293.0
30	40177.0
31	46807.0
32	60926.0
33	94983.0
34	155760.0
35	108684.0
36	101049.0
37	140223.0
38	155976.0
39	30956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.91577283057357	20.49012310429454	24.68629777738901	28.907806287742876
2	44.456840878745815	19.37559930616644	24.739110988613803	11.428448826473943
3	26.16861607931224	17.697872122711498	44.01634568887407	12.117166109102193
4	24.245802587517268	22.139133104146	40.53017862418284	13.08488568415389
5	17.678644812999973	26.248578581932268	41.50771815569665	14.56505844937111
6	16.806319100723048	34.939157530256196	38.97936818895247	9.275155180068294
7	68.6351415806664	5.151021027910132	23.71626762434623	2.497569767077234
8	69.89061063625066	3.967757534547267	23.05824802074239	3.0833838084596863
9	67.03432363605756	5.001741185557567	24.110798816323904	3.8531363620609693
10	40.371078825368166	21.687250065603912	28.153485094296336	9.788186014731586
11	36.124731601610144	17.713055920938622	32.90048505633684	13.261727421114392
12	32.30303560435643	17.52655926880109	38.47219631990942	11.698208806933055
13	17.373813559461897	32.80013995500974	38.383239067252674	11.442807418275681
14	12.557743819616476	37.06068072928443	38.69194879099007	11.689626660109026
15	11.5353130585616	21.95065595658754	54.78240131768962	11.73162966716124
16	12.754225469501195	15.409657225755021	55.69243896360675	16.143678341137036
17	12.35078204812934	15.887451746054277	39.84633005946437	31.91543614635201
18	16.621225299508673	21.98580975030904	44.13765103340601	17.25531391677628
19	22.293611746978506	23.284437101941048	37.29091332196749	17.13103782911295
20	24.145870089402866	19.76418901188132	36.58701224111211	19.5029286576037
21	20.57916287759383	22.78758031321535	39.873231788932	16.760025020258816
22	17.586634296569287	20.495404425417018	36.46950284613696	25.448458431876738
23	15.168614428899389	26.750799212422987	36.779862982725135	21.300723375952494
24	16.133445781462232	22.539523261743923	47.02414058876828	14.302890368025562
25	17.670145186818484	19.893498858739513	44.27933897664501	18.157016977796996
26	13.276333574843747	27.616193190726662	38.33075593859804	20.776717295831553
27	14.04352798852633	27.88735602210893	41.909841246787884	16.159274742576855
28	12.281217146469189	24.973634654708874	48.55201028537289	14.19313791344905
29	13.704863271547376	22.90096367606357	45.50559572477055	17.888577327618506
30	15.520317407399462	24.546012685073173	44.76967663461015	15.16399327291722
31	25.876327963440055	22.62303415199312	35.65667451713046	15.843963367436364
32	23.981736531393327	24.609388538542916	36.8895329166591	14.519342013404653
33	22.58928320919478	22.39659750886684	34.556509310804095	20.45760997113428
34	16.48011500076744	24.47900592333172	35.225669201150666	23.815209874750167
35	17.350790300193594	22.099853278297566	37.60506940811244	22.9442870133964
36	28.587543674050064	22.027730236718714	32.41790433877034	16.966821750460877
37	17.641840606427696	27.898991432706893	34.5422332396449	19.916934721220514
38	18.969762786160956	30.696606256054952	29.94690621859058	20.38672473919351
39	18.06632679164693	26.53410000511628	32.29956973736981	23.100003465866987
40	20.26715232815489	22.246822542659046	35.300928027145986	22.185097102040075
41	16.174210978876367	21.98250892460749	35.92610441501942	25.91717568149673
42	20.233731467926702	25.056402859175225	29.74984692420809	24.960018748689986
43	19.839035234663953	23.342779196215936	30.60484330155188	26.213342267568233
44	17.73731698984501	26.208968673513677	33.84674926432847	22.20696507231284
45	19.213693805505446	31.480057236317666	28.420934496764367	20.88531446141252
46	22.787167710002656	27.793612572184934	30.200574673754637	19.218645044057773
47	21.95643240156525	24.77253184884199	30.474378165698152	22.79665758389461
48	19.47338626757483	25.624144879841694	33.15250309865013	21.749965753933346
49	19.737452323698772	23.653716977301873	34.77164062590256	21.837190073096785
50	18.404826467340804	26.956275612344427	33.52665169192073	21.11224622839403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	925.0
1	1317.0
2	1709.0
3	23958.0
4	46207.0
5	32555.5
6	18904.0
7	19721.5
8	20539.0
9	22307.5
10	24076.0
11	24123.0
12	24170.0
13	23191.5
14	22213.0
15	20894.0
16	19575.0
17	18030.5
18	16486.0
19	15361.0
20	14236.0
21	13161.5
22	12087.0
23	11006.0
24	9925.0
25	9359.0
26	8793.0
27	9578.5
28	10364.0
29	11039.0
30	11714.0
31	13584.5
32	15455.0
33	16530.5
34	17606.0
35	19997.0
36	22388.0
37	25652.5
38	28917.0
39	36374.0
40	43831.0
41	63937.5
42	84044.0
43	97870.0
44	111696.0
45	115728.5
46	119761.0
47	115337.5
48	110914.0
49	109399.5
50	107885.0
51	93489.0
52	79093.0
53	66140.0
54	53187.0
55	46347.5
56	39508.0
57	36066.0
58	32624.0
59	28881.0
60	25138.0
61	21887.5
62	18637.0
63	16499.5
64	14362.0
65	11371.0
66	8380.0
67	6972.5
68	5565.0
69	4772.0
70	3979.0
71	3529.5
72	3080.0
73	2457.0
74	1834.0
75	1339.5
76	845.0
77	727.0
78	609.0
79	441.5
80	274.0
81	192.0
82	110.0
83	78.0
84	46.0
85	45.0
86	44.0
87	40.5
88	37.0
89	32.5
90	28.0
91	16.0
92	4.0
93	3.5
94	3.0
95	4.0
96	5.0
97	5.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1211818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.483101214748874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.055179330892	22.80219775646669
2	7.264454217180185	4.13828369475705
3	2.8381792577633194	2.4252044119342036
4	1.6581774671116232	1.889201465110251
5	1.153783387711562	1.643166450604213
6	0.8579811011877756	1.4662777527283866
7	0.6606921857839857	1.3172993679635252
8	0.5251372140116382	1.1966029134659784
9	0.4307072997161784	1.1041091650572379
>10	3.287416101494025	19.930831440616192
>50	0.7955231521087057	16.17813356320074
>100	0.46170013357168477	19.831099696717224
>500	0.006991043031962847	1.3570476854713667
>1k	0.0037868149756465417	1.9701916323797426
>5k	0.0	0.0
>10k+	2.912934596651186E-4	2.750353003527166
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33149	2.735476779516396	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	4682	0.38636164836633885	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3236	0.2670367992553337	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3005	0.24797453082888687	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1811	0.14944488363764194	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	1469	0.12122282388939594	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1446	0.1193248491110051	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	1352	0.11156790871236441	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1245	0.10273819996072017	No Hit
TAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1213	0.10009753939948078	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05726932592187936	0.0
2	0.0	0.0	0.0	0.24871721661173543	0.0
3	0.0	0.0	0.0	0.3453488890245895	0.0
4	0.0	0.0	0.0	0.5550338417155052	0.0
5	0.0	0.0	0.0	1.0264742725392757	0.0
6	8.252064253873106E-5	0.0	0.0	1.3781772510393475	0.0
7	8.252064253873106E-5	0.0	0.0	1.60172567167677	0.0
8	8.252064253873106E-5	0.0	0.0	2.0992426255427796	0.0
9	8.252064253873106E-5	0.0	0.0	2.2871421286034703	0.0
10	8.252064253873106E-5	0.0	0.0	2.73069058224915	0.0
11	8.252064253873106E-5	0.0	0.0	3.075379306133429	0.0
12	8.252064253873106E-5	0.0	0.0	3.4618234751423067	0.0
13	1.6504128507746212E-4	0.0	0.0	3.61638463861735	0.0
14	1.6504128507746212E-4	0.0	0.0	3.67753243473855	0.0
15	1.6504128507746212E-4	0.0	0.0	3.7584026644265065	0.0
16	1.6504128507746212E-4	0.0	0.0	3.9200606031598806	0.0
17	1.6504128507746212E-4	0.0	0.0	4.11002312228404	0.0
18	1.6504128507746212E-4	0.0	0.0	4.438290238303112	0.0
19	1.6504128507746212E-4	0.0	0.0	4.561988681468669	0.0
20	3.3008257015492424E-4	0.0	0.0	4.682798902145372	0.0
21	3.3008257015492424E-4	0.0	0.0	4.838680395901035	0.0
22	3.3008257015492424E-4	0.0	0.0	4.987465114398367	0.0
23	4.126032126936553E-4	0.0	0.0	5.1685154041283425	0.0
24	4.126032126936553E-4	0.0	0.0	5.307810248733721	0.0
25	4.126032126936553E-4	0.0	0.0	5.406339895924965	0.0
26	4.126032126936553E-4	0.0	0.0	5.50552970825652	0.0
27	4.126032126936553E-4	0.0	0.0	5.607112619221699	0.0
28	5.776444977711175E-4	0.0	0.0	5.73832044085828	0.0
29	5.776444977711175E-4	0.0	0.0	5.861606280811145	0.0
30	6.601651403098485E-4	0.0	0.0	6.0165800474988815	0.0
31	7.426857828485796E-4	0.0	0.0	6.161156213226739	0.0
32	8.252064253873106E-4	0.0	0.0	6.286505069243071	0.0
33	8.252064253873106E-4	0.0	0.0	6.433639374889629	0.0
34	8.252064253873106E-4	0.0	0.0	6.571861451142003	0.0
35	8.252064253873106E-4	0.0	0.0	6.777007768493289	0.0
36	8.252064253873106E-4	0.0	0.0	6.921088810365913	0.0
37	9.077270679260417E-4	0.0	0.0	7.054772251278657	0.0
38	9.902477104647728E-4	0.0	0.0	7.1958825500198875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACCGG	65	0.0	44.000004	2
TCGCACC	25	4.4429842E-5	44.0	11
GGTAATC	50	2.7284841E-11	44.0	8
CGTATAA	20	7.8566506E-4	44.0	27
GATCGGC	30	2.5278314E-6	44.0	34
ACGTTCC	20	7.8566506E-4	44.0	11
CGTTCGA	25	4.4429842E-5	44.0	14
TAGCCGT	70	0.0	44.0	44
ACGATCG	20	7.8566506E-4	44.0	32
CTAACGT	20	7.8566506E-4	44.0	1
TACGCGG	35	1.4461693E-7	44.0	2
ACGGATC	20	7.8566506E-4	44.0	5
ACAATCG	20	7.8566506E-4	44.0	2
ACTACCG	30	2.5278314E-6	44.0	1
CGAGAGT	40	8.312782E-9	44.0	38
TCGACTG	25	4.4429842E-5	44.0	1
TCGACAG	35	1.4461693E-7	44.0	1
TATCGTG	30	2.5278314E-6	44.0	1
TGTCGTG	20	7.8566506E-4	44.0	14
TAATGCG	20	7.8566506E-4	44.0	1
>>END_MODULE