##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1191719
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.015130244629816	31.0	28.0	33.0	25.0	33.0
2	28.820880593495616	31.0	28.0	33.0	16.0	33.0
3	28.252367378551487	31.0	27.0	33.0	16.0	34.0
4	31.89062522289231	33.0	32.0	35.0	25.0	37.0
5	33.713154695024585	35.0	33.0	35.0	30.0	37.0
6	33.60074648469983	35.0	33.0	37.0	28.0	37.0
7	34.0534328981916	35.0	33.0	37.0	30.0	37.0
8	34.623086482635586	35.0	35.0	37.0	32.0	37.0
9	36.58223037477795	39.0	37.0	39.0	32.0	39.0
10	36.44324039475749	39.0	35.0	39.0	32.0	39.0
11	36.511883254357784	39.0	35.0	39.0	32.0	39.0
12	35.415552659645435	37.0	35.0	39.0	30.0	39.0
13	35.31356385188119	37.0	34.0	39.0	29.0	39.0
14	36.16209609815737	38.0	35.0	40.0	29.0	41.0
15	36.68998815996053	38.0	35.0	40.0	31.0	41.0
16	36.329664123841276	38.0	34.0	40.0	31.0	41.0
17	35.76552358399925	37.0	34.0	40.0	30.0	41.0
18	35.45606892228789	37.0	34.0	39.0	29.0	40.0
19	34.83251420846693	37.0	33.0	39.0	27.0	40.0
20	35.00947370982589	35.0	34.0	39.0	30.0	40.0
21	35.17500518159063	35.0	34.0	39.0	30.0	40.0
22	35.45451822115784	35.0	34.0	39.0	31.0	40.0
23	35.8200624476072	35.0	35.0	40.0	31.0	41.0
24	35.46369404196795	35.0	34.0	39.0	31.0	41.0
25	35.16207595918165	35.0	34.0	39.0	30.0	41.0
26	35.19557546703543	35.0	34.0	39.0	30.0	41.0
27	35.5159723055519	35.0	34.0	40.0	31.0	41.0
28	35.45940360101668	35.0	35.0	40.0	31.0	41.0
29	35.52402453934191	36.0	35.0	40.0	31.0	41.0
30	35.15474788939339	36.0	34.0	40.0	30.0	41.0
31	34.38293758847514	35.0	34.0	39.0	26.0	41.0
32	34.17893899484694	35.0	34.0	39.0	24.0	41.0
33	33.642217670440765	35.0	33.0	40.0	21.0	41.0
34	33.004265267231624	35.0	33.0	40.0	16.0	41.0
35	32.54326145676959	35.0	33.0	40.0	12.0	41.0
36	32.11459580656178	35.0	32.0	40.0	10.0	41.0
37	32.00623469123174	35.0	31.0	40.0	10.0	41.0
38	31.86082793007412	35.0	31.0	40.0	10.0	41.0
39	31.66811807145812	35.0	31.0	39.0	10.0	40.0
40	31.480459739250612	35.0	30.0	39.0	10.0	40.0
41	31.197450909148884	35.0	30.0	39.0	8.0	40.0
42	31.367250165517206	35.0	30.0	39.0	8.0	40.0
43	31.138120647568766	35.0	30.0	39.0	8.0	40.0
44	30.90212709539749	35.0	29.0	39.0	8.0	40.0
45	30.784712671359607	35.0	29.0	38.0	8.0	40.0
46	30.593316880909008	35.0	29.0	38.0	8.0	40.0
47	30.588592612855884	35.0	29.0	38.0	8.0	40.0
48	30.567678286575948	35.0	29.0	38.0	7.0	40.0
49	30.64301232085752	35.0	29.0	38.0	8.0	40.0
50	30.68834767256375	35.0	29.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	11.0
14	44.0
15	131.0
16	409.0
17	911.0
18	1669.0
19	2957.0
20	4589.0
21	6875.0
22	9968.0
23	14275.0
24	21449.0
25	32910.0
26	45578.0
27	49022.0
28	42865.0
29	38508.0
30	39627.0
31	46349.0
32	60620.0
33	92674.0
34	152967.0
35	105810.0
36	97665.0
37	134246.0
38	155169.0
39	34414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.817579479726344	20.45566110802966	24.746605533687053	28.98015387855694
2	44.40384016701924	19.348269180905902	24.905367792239613	11.342522859835247
3	26.172528926701684	17.766184813701887	44.01314403814993	12.048142221446499
4	24.31991098572734	22.09606459240811	40.48487940529605	13.099145016568503
5	17.56513070614801	26.329864674474436	41.57120932031796	14.533795299059593
6	16.824100312238034	34.86006348812094	38.981337043380194	9.33449915626083
7	68.52236139559746	5.202568726352437	23.766173065966054	2.5088968120840565
8	69.79875289392885	3.975433806123759	23.115600237975563	3.110213061971824
9	66.99171532886527	5.028534411216066	24.141009751459865	3.8387405084587893
10	40.44535666545553	21.855068183019654	28.148749831126295	9.550825320398516
11	36.12571419940439	17.7434445536238	32.96867801889539	13.162163228076418
12	32.47821004783846	17.699054894652182	38.48868734995414	11.334047707555222
13	17.33101511346215	32.90171592464331	38.29921315343634	11.468055808458203
14	12.566888670903124	37.056890089022666	38.66280557748932	11.713415662584888
15	11.492054754518472	21.940323180212783	54.78237738930066	11.785244675968077
16	12.721790959110328	15.344305159186018	55.77094935970644	16.162954521997218
17	12.342339091681847	15.924978958966	39.87726972549737	31.855412223854785
18	16.56690880987884	21.96180475430869	44.23777752977002	17.233508906042445
19	22.308362961402814	23.286529794355886	37.23008528017091	17.17502196407039
20	24.167442157085688	19.84989750100485	36.672906952058334	19.30975338985113
21	20.577585823503693	22.77810456995315	39.875759302318755	16.7685503042244
22	17.581074061922315	20.50189683977515	36.446846949658436	25.4701821486441
23	15.156425298245644	26.805144501346373	36.82453665671186	21.213893543696123
24	16.23075574023742	22.425840319739805	47.09121865137671	14.252185288646066
25	17.56622156733257	19.892441087202602	44.39502936514396	18.146307980320863
26	13.20118249352406	27.793632559353338	38.35551837303928	20.64966657408332
27	13.974351336179083	27.903893451392488	41.96987712707442	16.151878085354014
28	12.374729277623333	24.92089158601986	48.58494326263154	14.119435873725267
29	13.613863670882145	22.898938424242626	45.633660284009906	17.85353762086532
30	15.550058361073374	24.64507153112437	44.75316748327416	15.0517026245281
31	25.89872276937768	22.614055830275426	35.61796027419216	15.86926112615474
32	23.982415317704927	24.620569110671223	36.875387570392014	14.521628001231834
33	22.62991527365092	22.437420230775878	34.55294410846852	20.379720387104676
34	16.455557056655135	24.487064484161113	35.182454924357174	23.874923534826582
35	17.36760091934424	22.14834201686807	37.57286742931849	22.9111896344692
36	28.50789489804224	21.976489424100816	32.54685039006679	16.96876528779016
37	17.5851018570653	27.996952301675144	34.55529365563526	19.862652185624295
38	18.968733401078612	30.67400956097872	30.04844262783425	20.308814410108422
39	18.129777237754872	26.52068146937323	32.40268888890754	22.946852403964357
40	20.249236606951808	22.317089850879277	35.38418033110155	22.049493211067375
41	16.184603920890748	21.989411933517886	36.03131275074073	25.79467139485063
42	20.164988558544422	25.044410637071323	29.82708171976783	24.963519084616422
43	19.802486995675995	23.34652715950656	30.666289620288005	26.184696224529443
44	17.75141623151095	26.21926813284004	33.964802105194266	22.064513530454747
45	19.149564620518763	31.62431747752616	28.520817407459308	20.705300494495766
46	22.772818088827986	27.971275107638633	30.28591471647259	18.969992087060792
47	21.920687678890747	24.79057563066461	30.533791942563642	22.754944747881005
48	19.553434996001574	25.645978624155525	33.19784278005134	21.602743599791562
49	19.614019747943935	23.680918068772925	34.999022420553835	21.7060397627293
50	18.354075079779715	27.08264280421811	33.652983631208365	20.910298484793817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	995.0
1	1360.0
2	1725.0
3	24201.0
4	46677.0
5	32861.0
6	19045.0
7	19910.5
8	20776.0
9	22335.0
10	23894.0
11	23726.0
12	23558.0
13	22475.0
14	21392.0
15	19945.5
16	18499.0
17	17400.5
18	16302.0
19	14887.5
20	13473.0
21	12620.0
22	11767.0
23	10718.5
24	9670.0
25	9087.5
26	8505.0
27	9454.0
28	10403.0
29	11012.0
30	11621.0
31	13471.5
32	15322.0
33	16418.5
34	17515.0
35	19898.0
36	22281.0
37	25568.5
38	28856.0
39	36148.0
40	43440.0
41	63433.5
42	83427.0
43	96749.5
44	110072.0
45	113682.5
46	117293.0
47	113151.0
48	109009.0
49	107643.5
50	106278.0
51	91849.0
52	77420.0
53	64229.5
54	51039.0
55	44678.0
56	38317.0
57	35073.5
58	31830.0
59	28158.5
60	24487.0
61	21585.0
62	18683.0
63	16252.0
64	13821.0
65	10979.0
66	8137.0
67	6788.5
68	5440.0
69	4655.5
70	3871.0
71	3475.5
72	3080.0
73	2474.5
74	1869.0
75	1361.5
76	854.0
77	707.5
78	561.0
79	400.5
80	240.0
81	175.0
82	110.0
83	81.0
84	52.0
85	49.5
86	47.0
87	42.0
88	37.0
89	26.5
90	16.0
91	9.0
92	2.0
93	2.5
94	3.0
95	3.5
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1191719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.940570636339444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59293746212899	22.238720913143627
2	7.471310165993637	4.175053388778925
3	2.8938224825541536	2.425651544484945
4	1.68101423697377	1.8787398811543148
5	1.1633426363407975	1.62522285524727
6	0.852581012271034	1.4292959997936365
7	0.661284660759561	1.2933669542276198
8	0.5365252762095368	1.1992657902491275
9	0.42494954285311853	1.0685999447070942
>10	3.4223548539534083	20.23512661491126
>50	0.813028851812558	16.222378781139216
>100	0.47504825636717884	19.980609917574093
>500	0.007867041188235413	1.4809250437964538
>1k	0.0036309420868778828	1.916739886564663
>5k	0.0	0.0
>10k+	3.0257850723982356E-4	2.83030248422776
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33478	2.809219287432692	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	4605	0.38641659652988664	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3333	0.27968002524084956	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3098	0.2599606115199976	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1864	0.15641271138582163	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	1438	0.12066602949185169	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	1405	0.11789692033105119	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1310	0.10992524244389827	No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1214	0.1018696521579332	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.054123497233827776	0.0
2	8.391239881213609E-5	0.0	0.0	0.24242292016826114	0.0
3	8.391239881213609E-5	0.0	0.0	0.3302792017245676	0.0
4	8.391239881213609E-5	0.0	0.0	0.5403119359513442	0.0
5	8.391239881213609E-5	0.0	0.0	1.0043475013824568	0.0
6	8.391239881213609E-5	0.0	0.0	1.348556161309839	0.0
7	8.391239881213609E-5	0.0	0.0	1.5674836098107021	0.0
8	8.391239881213609E-5	0.0	0.0	2.048469479801866	0.0
9	8.391239881213609E-5	0.0	0.0	2.2300559108313287	0.0
10	8.391239881213609E-5	0.0	0.0	2.6698407930057337	0.0
11	8.391239881213609E-5	0.0	0.0	3.0022178047006047	0.0
12	8.391239881213609E-5	0.0	0.0	3.3590972368486196	0.0
13	1.6782479762427217E-4	0.0	0.0	3.5145029994486956	0.0
14	1.6782479762427217E-4	0.0	0.0	3.575675138182743	0.0
15	2.5173719643640824E-4	0.0	0.0	3.6494341367386105	0.0
16	2.5173719643640824E-4	0.0	0.0	3.800896016594516	0.0
17	2.5173719643640824E-4	0.0	0.0	3.9813915864394205	0.0
18	2.5173719643640824E-4	0.0	0.0	4.2903570388657055	0.0
19	2.5173719643640824E-4	0.0	0.0	4.407666572405072	0.0
20	2.5173719643640824E-4	0.0	0.0	4.525731317533747	0.0
21	2.5173719643640824E-4	0.0	0.0	4.673752789038356	0.0
22	2.5173719643640824E-4	0.0	0.0	4.825298581293073	0.0
23	2.5173719643640824E-4	0.0	0.0	4.990186444958921	0.0
24	2.5173719643640824E-4	0.0	0.0	5.124278458260714	0.0
25	3.3564959524854434E-4	0.0	0.0	5.217421220942185	0.0
26	3.3564959524854434E-4	0.0	0.0	5.324493441826471	0.0
27	3.3564959524854434E-4	0.0	0.0	5.4233422476271675	0.0
28	3.3564959524854434E-4	0.0	0.0	5.543504802726146	0.0
29	3.3564959524854434E-4	0.0	0.0	5.659807387479766	0.0
30	3.3564959524854434E-4	0.0	0.0	5.810597968145175	0.0
31	3.3564959524854434E-4	0.0	0.0	5.954927294102049	0.0
32	4.195619940606804E-4	0.0	0.0	6.079537206338071	0.0
33	5.034743928728165E-4	0.0	0.0	6.225796517467624	0.0
34	5.034743928728165E-4	0.0	0.0	6.360895479555164	0.0
35	5.034743928728165E-4	0.0	0.0	6.567739542627079	0.0
36	5.873867916849526E-4	0.0	0.0	6.703929365899176	0.0
37	7.552115893092248E-4	0.0	0.0	6.835420094837793	0.0
38	7.552115893092248E-4	0.0	0.0	6.971274268514642	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.856598E-4	44.000004	33
CTATGCG	20	7.856598E-4	44.000004	1
GACGTAG	20	7.856598E-4	44.000004	1
TAGCGAT	20	7.856598E-4	44.000004	28
CGTCTCA	20	7.856598E-4	44.000004	18
CGTCTAA	20	7.856598E-4	44.000004	26
CTAAGCG	20	7.856598E-4	44.000004	14
ACAATCG	20	7.856598E-4	44.000004	2
ACGTCTA	20	7.856598E-4	44.000004	25
GTCGGAC	20	7.856598E-4	44.000004	27
AAACTCG	35	1.4461511E-7	44.0	1
CGTTCGA	25	4.442939E-5	44.0	14
CCGTAAT	45	4.802132E-10	44.0	35
TACGCAC	25	4.442939E-5	44.0	20
TCTACGG	30	2.527795E-6	44.0	2
GGGCGTA	25	4.442939E-5	44.0	7
TACTGGC	25	4.442939E-5	44.0	42
TATTACG	25	4.442939E-5	44.0	1
GTATAGA	45	4.802132E-10	44.0	9
CGTCGGA	25	4.442939E-5	44.0	26
>>END_MODULE