FastQCFastQC Report
Sat 18 Jun 2016
SRR3549149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445689
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103622.32493958791893No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7740.17366369822903416No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7420.16648380372860896No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6730.15100215621206717No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4700.1054547004749949No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC4490.10074289470909087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG600.044.0000042
ACGTAGG600.044.0000042
GACGAGG600.044.0000042
AATACGG302.5242152E-644.0000042
ATTTACC254.4384396E-544.041
TTGATCG254.4384396E-544.01
CTATGCG207.8512885E-444.01
CGTATGA254.4384396E-544.015
CGAGTTT207.8512885E-444.042
ATAGGCG207.8512885E-444.04
CTATACG207.8512885E-444.01
TAGCGAG408.292773E-944.01
TTATACG207.8512885E-444.01
CAGATCG207.8512885E-444.025
TGTTCGG454.783942E-1044.02
GTGTAAC207.8512885E-444.021
TTAACGG254.4384396E-544.02
CGTAATG254.4384396E-544.02
GCGGATC207.8512885E-444.030
CGGTCTA408.292773E-944.031
Produced by FastQC (version 0.11.5)