##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445689
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.086302331895112	31.0	28.0	33.0	25.0	33.0
2	28.820240122596697	31.0	28.0	33.0	16.0	33.0
3	28.379042336696664	31.0	28.0	33.0	16.0	34.0
4	31.971708971951294	35.0	32.0	35.0	25.0	37.0
5	33.70096861264245	35.0	33.0	35.0	30.0	37.0
6	33.60182773189376	35.0	33.0	37.0	28.0	37.0
7	34.23670092822574	35.0	33.0	37.0	30.0	37.0
8	34.730978327937194	35.0	35.0	37.0	32.0	37.0
9	36.6379852318545	39.0	37.0	39.0	32.0	39.0
10	36.294788518451206	38.0	35.0	39.0	31.0	39.0
11	36.42780055150565	39.0	35.0	39.0	32.0	39.0
12	35.17871430526667	37.0	34.0	39.0	29.0	39.0
13	35.231372100276204	37.0	34.0	39.0	27.0	39.0
14	36.05883474799692	38.0	34.0	40.0	29.0	41.0
15	36.69758733107616	38.0	35.0	40.0	31.0	41.0
16	36.362988541337124	38.0	34.0	40.0	31.0	41.0
17	35.879896071027105	37.0	34.0	40.0	30.0	41.0
18	35.527497874078115	37.0	34.0	39.0	29.0	40.0
19	34.913397010022685	37.0	33.0	39.0	27.0	40.0
20	35.08592538743384	35.0	34.0	39.0	29.0	40.0
21	35.2235931333284	35.0	34.0	39.0	30.0	40.0
22	35.601632528512035	35.0	34.0	39.0	31.0	40.0
23	35.970914696122186	36.0	35.0	40.0	31.0	41.0
24	35.56807100915661	35.0	34.0	40.0	31.0	41.0
25	35.21440062465082	35.0	34.0	39.0	30.0	41.0
26	35.29502635245653	35.0	34.0	39.0	30.0	41.0
27	35.6467289971258	35.0	34.0	40.0	31.0	41.0
28	35.58527583135325	36.0	35.0	40.0	31.0	41.0
29	35.7059765890565	36.0	35.0	40.0	31.0	41.0
30	35.35073560262874	36.0	35.0	40.0	30.0	41.0
31	34.60885954107012	35.0	34.0	39.0	27.0	41.0
32	34.43844474510253	35.0	34.0	40.0	25.0	41.0
33	33.96798888911326	35.0	33.0	40.0	22.0	41.0
34	33.43095297393474	35.0	33.0	40.0	18.0	41.0
35	33.071399563372665	35.0	33.0	40.0	15.0	41.0
36	32.60322108017025	35.0	33.0	40.0	12.0	41.0
37	32.48013525126265	35.0	32.0	40.0	10.0	41.0
38	32.36609608942559	35.0	32.0	40.0	10.0	41.0
39	32.160502054122944	35.0	31.0	39.0	10.0	40.0
40	31.946357213213698	35.0	31.0	39.0	10.0	40.0
41	31.671679130514775	35.0	31.0	39.0	10.0	40.0
42	31.846989717044845	35.0	31.0	39.0	10.0	40.0
43	31.64441348114941	35.0	31.0	39.0	10.0	40.0
44	31.44714812346726	35.0	30.0	39.0	10.0	40.0
45	31.344677566644005	35.0	30.0	39.0	10.0	40.0
46	31.16372627549704	35.0	30.0	38.0	8.0	40.0
47	31.14007525426923	35.0	30.0	38.0	8.0	40.0
48	31.136958731312642	35.0	30.0	38.0	8.0	40.0
49	31.196199592092245	35.0	30.0	38.0	8.0	40.0
50	31.350645853947483	35.0	30.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	4.0
14	19.0
15	33.0
16	124.0
17	288.0
18	635.0
19	1034.0
20	1670.0
21	2270.0
22	3462.0
23	4844.0
24	7234.0
25	11144.0
26	15480.0
27	16836.0
28	15154.0
29	13823.0
30	14275.0
31	16858.0
32	22466.0
33	34170.0
34	55804.0
35	40115.0
36	39113.0
37	55409.0
38	61575.0
39	11845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.810374498809708	21.544844050447733	25.765948901588327	26.87883254915423
2	41.41161213312422	19.848369603019144	26.913834534843804	11.826183729012833
3	26.33136559349681	17.391050710248628	43.519359912405285	12.758223783849276
4	23.866193691116454	22.75689999080076	40.282798094635496	13.094108223447293
5	18.235810172564275	27.58493029893042	40.644485280094415	13.534774248410889
6	17.130779534608212	34.9153782121614	38.12434231044517	9.829499942785215
7	72.09982745816028	4.2798902373628245	21.24149350780477	2.378788796672119
8	73.01100094460486	3.4396182091099394	20.823264653155	2.726116193130187
9	69.90659405998353	4.900277996540189	21.5991420026072	3.5939859408690813
10	39.62830583658111	25.108764183096284	25.800277772168485	9.462652208154116
11	36.251287332646754	17.43502756406373	33.98446001584064	12.32922508744887
12	32.87651254574378	18.31837895931917	36.37783297321675	12.427275521720302
13	18.146061491308963	33.1221995606802	37.10030985732203	11.631429090688798
14	13.508074015737431	36.130350984655216	37.34038757968	13.02118741992735
15	11.599568308843162	22.154686339577598	53.0910567682846	13.15468858329463
16	14.293374976721434	16.407629535393514	52.64478145074256	16.65421403714249
17	14.121730623820646	17.075808467339332	38.843677990706524	29.958782918133497
18	17.67622714493739	21.33213967587264	43.10718909374025	17.88444408544972
19	22.79122886138092	22.262384757083975	36.7742977726621	18.172088608873
20	25.676200220333012	20.128385488535727	35.917197866673845	18.278216424457412
21	20.34782101420497	24.421064912977435	38.103924485459586	17.127189587358004
22	18.910944627307384	20.096075963283813	36.230645135958035	24.76233427345077
23	16.158352573206876	26.80680923244684	36.15548061540671	20.879357578939576
24	16.648604744564036	22.066283888541115	46.35093080601047	14.934180560884384
25	16.637161787703985	21.676774611893045	43.892490054724256	17.79357354567871
26	14.773081678031094	28.447863869200273	37.58136278885052	19.19769166391811
27	14.68064053633812	29.13062696185008	39.924476484723655	16.26425601708815
28	13.156483556919735	24.12175306099096	47.143860404901176	15.57790297718813
29	13.412940413606798	21.82687928129256	45.16288263789324	19.597297667207403
30	16.090592318859116	25.607991222578974	42.39323833435422	15.908178124207687
31	25.15588224075532	23.095028147430156	34.97887540414953	16.770214207664985
32	24.868686460738317	23.534796685581203	35.01656985027676	16.57994700340372
33	22.999670173596385	23.402866124135887	34.177868423945846	19.41959527832188
34	17.153889820031456	24.69031095674338	34.63401609642599	23.521783126799182
35	17.78773988139712	22.970726223891546	37.041300099396665	22.20023379531467
36	26.99303774605162	23.292250874488712	32.41139000513812	17.303321374321555
37	18.34687416561773	28.294393624253683	33.835477204956824	19.52325500517177
38	18.684104835434574	30.583209367967353	30.202226216038536	20.530459580559537
39	18.786193960362496	27.85507382950892	31.80670826518043	21.55202394494816
40	21.18113751966057	24.374619970427812	32.68916217362331	21.75508033628831
41	16.480999082319734	23.247600905564195	34.18011214097723	26.091287871138842
42	20.043572984749456	25.025971024638256	29.46494079952613	25.465515191086162
43	19.872377375254942	23.86911052325725	30.67744548328543	25.58106661820238
44	18.064614563069764	26.636959853171156	32.834330665553786	22.464094918205298
45	17.79671474952265	32.17579971684291	28.226408998202785	21.80107653543166
46	21.65770301712629	28.750990040140096	29.635687665614363	19.955619277119247
47	20.63636302444081	25.33134091260947	30.698985166786706	23.33331089616302
48	19.67201344435245	24.74393579379343	33.70309790010523	21.880952861748888
49	19.11803970930402	24.275896421046962	34.91358323853629	21.692480631112726
50	18.41126884441842	27.728303817235783	32.64877526705842	21.211652071287375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	368.0
2	537.0
3	7653.0
4	14769.0
5	10729.5
6	6690.0
7	7015.0
8	7340.0
9	7609.0
10	7878.0
11	7892.0
12	7906.0
13	7576.5
14	7247.0
15	6790.5
16	6334.0
17	5761.5
18	5189.0
19	4887.0
20	4585.0
21	4408.0
22	4231.0
23	3758.5
24	3286.0
25	3281.5
26	3277.0
27	3664.0
28	4051.0
29	4278.5
30	4506.0
31	5462.5
32	6419.0
33	7754.5
34	9090.0
35	9865.0
36	10640.0
37	11966.5
38	13293.0
39	15948.0
40	18603.0
41	25232.0
42	31861.0
43	35500.5
44	39140.0
45	41621.5
46	44103.0
47	42249.5
48	40396.0
49	39279.5
50	38163.0
51	33065.0
52	27967.0
53	24165.5
54	20364.0
55	18074.5
56	15785.0
57	14185.0
58	12585.0
59	10473.5
60	8362.0
61	7558.5
62	6755.0
63	5717.5
64	4680.0
65	3845.5
66	3011.0
67	2538.0
68	2065.0
69	1828.5
70	1592.0
71	1403.5
72	1215.0
73	1032.5
74	850.0
75	577.5
76	305.0
77	238.5
78	172.0
79	169.5
80	167.0
81	100.5
82	34.0
83	25.0
84	16.0
85	13.0
86	10.0
87	11.5
88	13.0
89	7.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	445689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.298021418335885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42624237966209	27.682911832769136
2	7.914943399190929	5.587636832591154
3	3.3738785041995567	3.5727370711229693
4	1.9227308222421962	2.7147437498079845
5	1.2397340115673254	2.188007884667146
6	0.9064571902093582	1.9197687188888697
7	0.6845350110253478	1.691391203654146
8	0.47304725634734646	1.335810574914693
9	0.3858198467401624	1.2256809492467988
>10	3.9741978352789262	31.19305784801561
>50	0.6422342102358424	15.023903533659277
>100	0.05362591815090533	3.036058440836217
>500	0.0019152113625323331	0.49327780947008676
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.384037875107777E-4	2.3350135503558884
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10362	2.32493958791893	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	774	0.17366369822903416	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	742	0.16648380372860896	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	673	0.15100215621206717	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	470	0.1054547004749949	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	449	0.10074289470909087	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059682873034784346	0.0
2	0.0	0.0	0.0	0.22818602209163788	0.0
3	0.0	0.0	0.0	0.30469677286179375	0.0
4	0.0	0.0	0.0	0.46871248785588154	0.0
5	0.0	0.0	0.0	0.8701134647702771	0.0
6	0.0	0.0	0.0	1.1629185373657416	0.0
7	2.24371703138287E-4	0.0	0.0	1.3448839886108923	0.0
8	2.24371703138287E-4	0.0	0.0	1.7561573204633725	0.0
9	2.24371703138287E-4	0.0	0.0	1.884049191252196	0.0
10	2.24371703138287E-4	0.0	0.0	2.2367615085855834	0.0
11	2.24371703138287E-4	0.0	0.0	2.528893466071633	0.0
12	2.24371703138287E-4	0.0	0.0	2.8239422556984803	0.0
13	2.24371703138287E-4	0.0	0.0	2.932089416611135	0.0
14	2.24371703138287E-4	0.0	0.0	2.9771881289419304	0.0
15	2.24371703138287E-4	0.0	0.0	3.0492114456493207	0.0
16	2.24371703138287E-4	0.0	0.0	3.201335460377079	0.0
17	2.24371703138287E-4	0.0	0.0	3.409328029186271	0.0
18	2.24371703138287E-4	0.0	0.0	3.6871001976714703	0.0
19	2.24371703138287E-4	0.0	0.0	3.80242725308455	0.0
20	2.24371703138287E-4	0.0	0.0	3.9309922389827885	0.0
21	2.24371703138287E-4	0.0	0.0	4.088725546289004	0.0
22	2.24371703138287E-4	0.0	0.0	4.247131968704635	0.0
23	2.24371703138287E-4	0.0	0.0	4.442335350434945	0.0
24	2.24371703138287E-4	0.0	0.0	4.588176957474831	0.0
25	2.24371703138287E-4	0.0	0.0	4.718985660404452	0.0
26	2.24371703138287E-4	0.0	0.0	4.831620255379873	0.0
27	2.24371703138287E-4	0.0	0.0	4.953678461887101	0.0
28	2.24371703138287E-4	0.0	0.0	5.097276351895604	0.0
29	2.24371703138287E-4	0.0	0.0	5.237733038060172	0.0
30	2.24371703138287E-4	0.0	0.0	5.397485690694633	0.0
31	4.48743406276574E-4	0.0	0.0	5.561052662282443	0.0
32	4.48743406276574E-4	0.0	0.0	5.693207595430895	0.0
33	4.48743406276574E-4	0.0	0.0	5.8460047252680685	0.0
34	4.48743406276574E-4	0.0	0.0	5.987358898245189	0.0
35	4.48743406276574E-4	0.0	0.0	6.2070187956175715	0.0
36	4.48743406276574E-4	0.0	0.0	6.358918438642192	0.0
37	4.48743406276574E-4	0.0	0.0	6.51642737424527	0.0
38	4.48743406276574E-4	0.0	0.0	6.680891832645634	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	60	0.0	44.000004	2
ACGTAGG	60	0.0	44.000004	2
GACGAGG	60	0.0	44.000004	2
AATACGG	30	2.5242152E-6	44.000004	2
ATTTACC	25	4.4384396E-5	44.0	41
TTGATCG	25	4.4384396E-5	44.0	1
CTATGCG	20	7.8512885E-4	44.0	1
CGTATGA	25	4.4384396E-5	44.0	15
CGAGTTT	20	7.8512885E-4	44.0	42
ATAGGCG	20	7.8512885E-4	44.0	4
CTATACG	20	7.8512885E-4	44.0	1
TAGCGAG	40	8.292773E-9	44.0	1
TTATACG	20	7.8512885E-4	44.0	1
CAGATCG	20	7.8512885E-4	44.0	25
TGTTCGG	45	4.783942E-10	44.0	2
GTGTAAC	20	7.8512885E-4	44.0	21
TTAACGG	25	4.4384396E-5	44.0	2
CGTAATG	25	4.4384396E-5	44.0	2
GCGGATC	20	7.8512885E-4	44.0	30
CGGTCTA	40	8.292773E-9	44.0	31
>>END_MODULE