FastQCFastQC Report
Sat 18 Jun 2016
SRR3549148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438183
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104862.393064085096866No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7750.17686674289052748No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7590.1732153004566585No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6120.1396676730954875No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4500.10269681845256433No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC4440.10132752753986347No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG207.851144E-444.03
AAATCGT302.524117E-644.012
CGAACGG207.851144E-444.02
AGTTTAG254.4383163E-544.01
CGCGGTA207.851144E-444.031
ACGCATT207.851144E-444.035
TGGAATC207.851144E-444.022
TTAGACT207.851144E-444.012
GGCAACG207.851144E-444.01
CGACATA207.851144E-444.021
ACGGGTA502.7284841E-1144.05
ATGTACG302.524117E-644.01
TATTGCG254.4383163E-544.01
GGGACGT351.4433499E-744.07
GAGGTTC207.851144E-444.09
GTAGAAC207.851144E-444.032
AGCGCTT302.524117E-644.037
GGAACGA254.4383163E-544.08
TATTACT254.4383163E-544.043
GACGGTC302.524117E-644.029
Produced by FastQC (version 0.11.5)