##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438183
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.965920631334395	31.0	28.0	31.0	23.0	33.0
2	28.727956127919157	31.0	28.0	33.0	16.0	33.0
3	28.26080199368757	31.0	27.0	33.0	16.0	34.0
4	31.875262162155995	35.0	32.0	35.0	25.0	37.0
5	33.635757206463964	35.0	33.0	35.0	28.0	37.0
6	33.53844854775288	35.0	33.0	37.0	28.0	37.0
7	34.15689335277727	35.0	33.0	37.0	30.0	37.0
8	34.694134642375445	35.0	35.0	37.0	32.0	37.0
9	36.60595230759751	39.0	37.0	39.0	32.0	39.0
10	36.36101354913358	39.0	35.0	39.0	32.0	39.0
11	36.4326023602011	39.0	35.0	39.0	32.0	39.0
12	35.33757357085966	37.0	34.0	39.0	30.0	39.0
13	35.293267881227706	37.0	34.0	39.0	29.0	39.0
14	36.105499300520556	38.0	34.0	40.0	29.0	41.0
15	36.65203807541598	38.0	35.0	40.0	31.0	41.0
16	36.29614110999286	38.0	34.0	40.0	31.0	41.0
17	35.82549984823692	37.0	34.0	40.0	30.0	41.0
18	35.44984401494353	37.0	34.0	39.0	29.0	40.0
19	34.816533274910256	37.0	33.0	39.0	27.0	40.0
20	35.16904124532444	35.0	34.0	39.0	30.0	40.0
21	35.29508447383856	35.0	34.0	39.0	30.0	40.0
22	35.619006214298594	35.0	34.0	39.0	31.0	40.0
23	35.96517208563545	35.0	35.0	40.0	31.0	41.0
24	35.57996316607445	35.0	34.0	40.0	31.0	41.0
25	35.23798276062741	35.0	34.0	39.0	30.0	41.0
26	35.31096368412285	35.0	34.0	39.0	30.0	41.0
27	35.644922326972974	35.0	34.0	40.0	31.0	41.0
28	35.593243005776124	36.0	35.0	40.0	31.0	41.0
29	35.70344353843029	36.0	35.0	40.0	31.0	41.0
30	35.34671358770194	36.0	35.0	40.0	30.0	41.0
31	34.58531709354311	35.0	34.0	39.0	27.0	41.0
32	34.43933242503703	35.0	34.0	40.0	25.0	41.0
33	33.9822973506503	35.0	33.0	40.0	22.0	41.0
34	33.44492369626389	35.0	33.0	40.0	18.0	41.0
35	33.04335175029611	35.0	33.0	40.0	15.0	41.0
36	32.616671573292436	35.0	33.0	40.0	12.0	41.0
37	32.528080733392216	35.0	32.0	40.0	10.0	41.0
38	32.396033164225905	35.0	32.0	40.0	10.0	41.0
39	32.158404137084275	35.0	31.0	40.0	10.0	40.0
40	31.98716517984495	35.0	31.0	39.0	10.0	40.0
41	31.71148812254241	35.0	31.0	39.0	10.0	40.0
42	31.902873913410605	35.0	31.0	39.0	10.0	40.0
43	31.69855516987195	35.0	31.0	39.0	10.0	40.0
44	31.518422668154628	35.0	30.0	39.0	10.0	40.0
45	31.440519143828034	35.0	30.0	39.0	10.0	40.0
46	31.251979195906735	35.0	30.0	39.0	8.0	40.0
47	31.237181725443477	35.0	30.0	38.0	9.0	40.0
48	31.21674734072294	35.0	30.0	38.0	8.0	40.0
49	31.280316671345076	35.0	30.0	38.0	8.0	40.0
50	31.36219798577307	35.0	30.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	3.0
13	4.0
14	14.0
15	39.0
16	128.0
17	269.0
18	607.0
19	1032.0
20	1558.0
21	2291.0
22	3351.0
23	4907.0
24	6961.0
25	11013.0
26	15267.0
27	16703.0
28	15050.0
29	13795.0
30	13972.0
31	16744.0
32	21804.0
33	33439.0
34	54279.0
35	39363.0
36	38189.0
37	53278.0
38	60543.0
39	13574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.746548816362115	21.538489626480263	25.746320601209998	26.96864095594763
2	41.39754394853291	19.605507288050884	27.16741635344137	11.829532409974828
3	26.419555299954585	17.583977470600182	43.39260080833807	12.603866421107163
4	23.898006084215957	22.701017611363287	40.145555624020105	13.255420680400654
5	18.34393392714916	27.56222856660345	40.57779512212934	13.516042384118052
6	17.15995371796715	34.88451172227129	38.09641177316327	9.859122786598293
7	72.0173991231974	4.358452975126831	21.19434117708811	2.429806724587672
8	72.84969978296739	3.514969772903102	20.812309012444572	2.823021431684935
9	69.77016452030315	5.02370014354733	21.576601556883766	3.6295337792657407
10	39.55059872245158	25.423852591268947	25.85015849542315	9.175390190856332
11	36.22618860156601	17.567774194799888	33.79295864969659	12.413078553937511
12	32.907940289787604	18.61779210968933	36.44847015972778	12.025797440795284
13	18.083312223431765	33.27217167256603	36.934112003432354	11.710404100569853
14	13.521063117464621	36.28027559261769	37.22234774055589	12.976313549361794
15	11.565031048671445	22.16311449782397	53.140354600703354	13.131499852801227
16	14.163260555521322	16.43879383727803	52.7131358359407	16.684809771259953
17	14.24564622543549	16.9474854113464	38.77649292647136	30.03037543674675
18	17.705616146678445	21.449942147458938	42.95648165264285	17.88796005321977
19	22.789336875232493	22.287263540575513	36.771851030277304	18.15154855391469
20	25.61349938267801	20.393077778005996	35.85899042181006	18.134432417505927
21	20.294717047443648	24.379996485486657	38.14502160056415	17.180264866505546
22	18.97243845607885	20.29380418683518	36.155213689257685	24.57854366782828
23	16.125454433421652	26.73905651291812	36.1159606830936	21.019528370566633
24	16.590556913435712	22.114048240118855	46.32448086758272	14.970913978862713
25	16.528025961755706	21.76761763920554	43.912703140012276	17.79165325902648
26	14.738819169159918	28.32150037769608	37.586122693030084	19.353557760113926
27	14.662367093200787	29.230024898273093	39.875805314218034	16.231802694308087
28	13.165732125618748	24.359457121796144	46.978089063245264	15.496721689339843
29	13.366789674633658	21.86050120611708	45.18888227064948	19.583826848599788
30	15.99765394823624	25.539785888544287	42.54843296065799	15.914127202561486
31	25.200201742194473	23.071182588096754	34.8982046313983	16.830411038310476
32	24.86335617767006	23.465994801258834	35.02075616808503	16.64989285298608
33	23.020062394022588	23.448650449697958	34.10606983840085	19.425217317878605
34	17.18003665135343	24.701323419667126	34.77405558864675	23.34458434033269
35	17.89457829263116	22.89682621187951	36.931145206454836	22.27745028903449
36	26.93783191041186	23.32039353420831	32.55831467674465	17.183459878635183
37	18.269307572406962	28.340442235321774	33.92943131066244	19.460818881608827
38	18.707708879623354	30.620083389816582	30.25767772825509	20.41453000230497
39	18.91949254078775	27.825132421842014	31.887818559825458	21.36755647754477
40	21.110358001109127	24.382506852159942	32.71760885292218	21.789526293808752
41	16.349789927952475	23.275206934089184	34.40183667554423	25.973166462414106
42	20.17946839562466	24.981343411314448	29.459609341302606	25.379578851758282
43	20.027020674010632	23.755828044447185	30.648610283831186	25.568540997711004
44	17.942047044271458	26.657811918764533	32.95449618081943	22.44564485614458
45	17.874951789549115	32.30180084576536	28.23021431684935	21.593033047836176
46	21.553095396215735	28.939507009628397	29.769297302725118	19.738100291430747
47	20.530919729884545	25.494827503577273	30.769564314453095	23.204688452085087
48	19.83029921288594	24.729165668225374	33.66515816451117	21.77537695437751
49	19.166421335378143	24.437278488667978	34.88542458287975	21.51087559307413
50	18.413083118240554	27.787020491438508	32.70003628620919	21.09986010411175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	209.0
1	377.5
2	546.0
3	7750.5
4	14955.0
5	10822.5
6	6690.0
7	6850.0
8	7010.0
9	7393.5
10	7777.0
11	7726.0
12	7675.0
13	7393.5
14	7112.0
15	6595.0
16	6078.0
17	5614.5
18	5151.0
19	4814.0
20	4477.0
21	4237.5
22	3998.0
23	3612.5
24	3227.0
25	3229.0
26	3231.0
27	3566.0
28	3901.0
29	4182.0
30	4463.0
31	5452.5
32	6442.0
33	7649.5
34	8857.0
35	9570.0
36	10283.0
37	11832.5
38	13382.0
39	15968.0
40	18554.0
41	25074.0
42	31594.0
43	35121.5
44	38649.0
45	41086.5
46	43524.0
47	41480.5
48	39437.0
49	38601.5
50	37766.0
51	32544.5
52	27323.0
53	23636.0
54	19949.0
55	17643.0
56	15337.0
57	13748.5
58	12160.0
59	10151.5
60	8143.0
61	7300.0
62	6457.0
63	5467.0
64	4477.0
65	3760.5
66	3044.0
67	2530.5
68	2017.0
69	1787.5
70	1558.0
71	1372.0
72	1186.0
73	1023.5
74	861.0
75	592.0
76	323.0
77	229.5
78	136.0
79	140.0
80	144.0
81	89.0
82	34.0
83	29.5
84	25.0
85	18.0
86	11.0
87	9.0
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	438183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.01947869044326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.27681920334338	27.41213402047163
2	7.767456792060802	5.440245752170239
3	3.44594262184134	3.6202534264218897
4	1.994993923404171	2.7945458875286455
5	1.2799926681093161	2.24123379823889
6	0.9204372695483612	1.9339940008103167
7	0.656003845348904	1.6081038878131344
8	0.5150655458439455	1.442986152549086
9	0.427779583491998	1.3482556207474243
>10	4.006103709744866	31.176340973993742
>50	0.6538293430994687	15.094232906270664
>100	0.05296017679105697	2.99535450267483
>500	0.001961488029298406	0.49136452856904034
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.538293430994686E-4	2.400954541740427
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10486	2.393064085096866	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	775	0.17686674289052748	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	759	0.1732153004566585	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	612	0.1396676730954875	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	450	0.10269681845256433	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	444	0.10132752753986347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05408699105168389	0.0
2	0.0	0.0	0.0	0.22365084907447344	0.0
3	2.2821515211680964E-4	0.0	0.0	0.2987336341209038	0.0
4	2.2821515211680964E-4	0.0	0.0	0.46943856790427746	0.0
5	2.2821515211680964E-4	0.0	0.0	0.8754333235200817	0.0
6	2.2821515211680964E-4	0.0	0.0	1.142673266648866	0.0
7	2.2821515211680964E-4	0.0	0.0	1.329581476232533	0.0
8	2.2821515211680964E-4	0.0	0.0	1.7223397530255624	0.0
9	2.2821515211680964E-4	0.0	0.0	1.8645177927943348	0.0
10	2.2821515211680964E-4	0.0	0.0	2.220533430096558	0.0
11	2.2821515211680964E-4	0.0	0.0	2.5014662823523506	0.0
12	2.2821515211680964E-4	0.0	0.0	2.7935816770618667	0.0
13	2.2821515211680964E-4	0.0	0.0	2.908145683424505	0.0
14	2.2821515211680964E-4	0.0	0.0	2.941693310785676	0.0
15	2.2821515211680964E-4	0.0	0.0	3.0128964382461207	0.0
16	2.2821515211680964E-4	0.0	0.0	3.14959731436409	0.0
17	2.2821515211680964E-4	0.0	0.0	3.344493054271845	0.0
18	2.2821515211680964E-4	0.0	0.0	3.6224591095501193	0.0
19	2.2821515211680964E-4	0.0	0.0	3.7361102553042906	0.0
20	2.2821515211680964E-4	0.0	0.0	3.8602592980558352	0.0
21	2.2821515211680964E-4	0.0	0.0	4.0159020317995	0.0
22	2.2821515211680964E-4	0.0	0.0	4.178163004954551	0.0
23	2.2821515211680964E-4	0.0	0.0	4.365527644842452	0.0
24	2.2821515211680964E-4	0.0	0.0	4.505423533090056	0.0
25	4.564303042336193E-4	0.0	0.0	4.634136878883937	0.0
26	4.564303042336193E-4	0.0	0.0	4.745962303421173	0.0
27	4.564303042336193E-4	0.0	0.0	4.866459903738849	0.0
28	4.564303042336193E-4	0.0	0.0	4.9965425404454304	0.0
29	4.564303042336193E-4	0.0	0.0	5.130961265042231	0.0
30	4.564303042336193E-4	0.0	0.0	5.2971018957832685	0.0
31	4.564303042336193E-4	0.0	0.0	5.455939641656568	0.0
32	4.564303042336193E-4	0.0	0.0	5.587391569275851	0.0
33	4.564303042336193E-4	0.0	0.0	5.745772884844916	0.0
34	4.564303042336193E-4	0.0	0.0	5.879506963985367	0.0
35	4.564303042336193E-4	0.0	0.0	6.081933803912977	0.0
36	4.564303042336193E-4	0.0	0.0	6.228447931571969	0.0
37	6.846454563504289E-4	0.0	0.0	6.382721374402932	0.0
38	6.846454563504289E-4	0.0	0.0	6.536081956625428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	20	7.851144E-4	44.0	3
AAATCGT	30	2.524117E-6	44.0	12
CGAACGG	20	7.851144E-4	44.0	2
AGTTTAG	25	4.4383163E-5	44.0	1
CGCGGTA	20	7.851144E-4	44.0	31
ACGCATT	20	7.851144E-4	44.0	35
TGGAATC	20	7.851144E-4	44.0	22
TTAGACT	20	7.851144E-4	44.0	12
GGCAACG	20	7.851144E-4	44.0	1
CGACATA	20	7.851144E-4	44.0	21
ACGGGTA	50	2.7284841E-11	44.0	5
ATGTACG	30	2.524117E-6	44.0	1
TATTGCG	25	4.4383163E-5	44.0	1
GGGACGT	35	1.4433499E-7	44.0	7
GAGGTTC	20	7.851144E-4	44.0	9
GTAGAAC	20	7.851144E-4	44.0	32
AGCGCTT	30	2.524117E-6	44.0	37
GGAACGA	25	4.4383163E-5	44.0	8
TATTACT	25	4.4383163E-5	44.0	43
GACGGTC	30	2.524117E-6	44.0	29
>>END_MODULE