FastQCFastQC Report
Sat 18 Jun 2016
SRR3549145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161594
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21801.3490599898511084No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA2300.14233201727786923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG254.425818E-544.03
CTGCTCA408.236384E-944.044
TCGTTTG207.8363833E-444.01
ATGATTG254.425818E-544.01
AATTTCG207.8363833E-444.037
CACGGGA302.514178E-644.04
TATGGGC254.425818E-544.04
GCAGTGT551.8189894E-1244.043
TTCACCC207.8363833E-444.044
TGAACGG254.425818E-544.02
ATTCGCC207.8363833E-444.014
CCTAAGT207.8363833E-444.020
ACGGGAG502.7284841E-1144.05
GTTACAC207.8363833E-444.033
GTTGTAG207.8363833E-444.01
GTTCGGG254.425818E-544.03
CTACGGG302.514178E-644.03
TGATTCC207.8363833E-444.023
CAAGGGC254.425818E-544.04
TGCGAAG207.8363833E-444.01
Produced by FastQC (version 0.11.5)