##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	161594
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.12981917645457	31.0	28.0	33.0	25.0	33.0
2	28.675192148223324	31.0	28.0	33.0	16.0	33.0
3	28.198769756302834	31.0	27.0	33.0	16.0	34.0
4	32.1725002165922	35.0	32.0	35.0	25.0	37.0
5	33.68260578981893	35.0	33.0	35.0	28.0	37.0
6	33.51642387712415	35.0	33.0	37.0	28.0	37.0
7	34.48110697179351	35.0	35.0	37.0	32.0	37.0
8	34.849920170303356	35.0	35.0	37.0	32.0	37.0
9	36.7394767132443	39.0	37.0	39.0	32.0	39.0
10	36.47877396437987	39.0	35.0	39.0	32.0	39.0
11	36.40049754322561	39.0	35.0	39.0	32.0	39.0
12	34.857903139968066	35.0	33.0	39.0	29.0	39.0
13	34.606854214884216	37.0	33.0	39.0	25.0	39.0
14	35.31606990358553	37.0	33.0	40.0	26.0	41.0
15	36.13194796836516	38.0	34.0	40.0	31.0	41.0
16	35.813600752503184	36.0	34.0	40.0	31.0	41.0
17	35.341906258895754	36.0	33.0	40.0	29.0	41.0
18	35.08400683193683	36.0	33.0	39.0	29.0	40.0
19	34.49936260009654	36.0	33.0	39.0	27.0	40.0
20	34.86683292696511	35.0	34.0	39.0	29.0	40.0
21	34.95713949775363	35.0	34.0	39.0	30.0	40.0
22	35.37859078926198	35.0	34.0	39.0	30.0	40.0
23	35.79709642684753	35.0	34.0	40.0	31.0	41.0
24	35.36253821305247	35.0	34.0	39.0	31.0	41.0
25	35.01382477072168	35.0	34.0	39.0	30.0	41.0
26	35.13364976422392	35.0	34.0	39.0	30.0	41.0
27	35.44643984306348	35.0	34.0	40.0	31.0	41.0
28	35.44806119039073	36.0	34.0	40.0	31.0	41.0
29	35.61571593004691	36.0	35.0	40.0	31.0	41.0
30	35.279447256705076	36.0	34.0	40.0	30.0	41.0
31	34.601612683639246	35.0	34.0	39.0	29.0	40.0
32	34.49673255195118	35.0	34.0	39.0	27.0	40.0
33	34.1840600517346	35.0	33.0	40.0	24.0	41.0
34	33.82694283203584	35.0	34.0	40.0	23.0	41.0
35	33.59067168335458	35.0	33.0	40.0	21.0	41.0
36	33.161806750250626	35.0	33.0	39.0	18.0	41.0
37	33.04507593103704	35.0	33.0	39.0	18.0	41.0
38	32.915132987610924	35.0	33.0	39.0	18.0	40.0
39	32.731988811465776	35.0	33.0	39.0	18.0	40.0
40	32.55505773729222	35.0	32.0	39.0	16.0	40.0
41	32.288766909662485	35.0	32.0	38.0	15.0	40.0
42	32.440195799348984	35.0	32.0	39.0	15.0	40.0
43	32.2164436798396	35.0	32.0	38.0	15.0	40.0
44	31.88241518868275	35.0	31.0	38.0	12.0	40.0
45	31.838960604972957	35.0	31.0	38.0	12.0	40.0
46	31.705180885428913	35.0	31.0	38.0	12.0	40.0
47	31.734451774199538	35.0	31.0	38.0	12.0	40.0
48	31.811942275084473	35.0	31.0	38.0	12.0	40.0
49	31.8965493768333	35.0	31.0	38.0	12.0	40.0
50	31.997140982957287	35.0	32.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	5.0
15	15.0
16	30.0
17	88.0
18	195.0
19	319.0
20	542.0
21	780.0
22	1085.0
23	1552.0
24	2153.0
25	3254.0
26	4554.0
27	5243.0
28	5018.0
29	4905.0
30	5409.0
31	6571.0
32	9221.0
33	14711.0
34	24639.0
35	15200.0
36	13903.0
37	18674.0
38	19670.0
39	3855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.708256494671833	23.898164535811972	27.958958872235357	22.43462009728084
2	36.488978551183834	21.05214302511232	30.06423505823236	12.39464336547149
3	27.550527866133645	18.927682958525686	40.29543176108024	13.22635741426043
4	24.43098134831739	25.402552074953277	35.86333651001893	14.303130066710398
5	20.053962399594045	29.347624292981173	37.28356250850898	13.314850798915801
6	18.0866863868708	36.873893832691806	34.80512890330086	10.234290877136527
7	77.67120066339098	4.8819881926309145	15.126180427491121	2.320630716486998
8	79.46520291594985	3.3497530848917654	14.723937769966708	2.4611062291916777
9	76.61423072638836	4.529252323724891	15.749965964082824	3.106550985803928
10	52.10837036028565	18.977189747144077	19.70803371412305	9.206406178447219
11	46.96894686683912	16.18500686906692	26.502840451996978	10.343205812096985
12	41.60055447603253	18.438803420919093	28.16131787071302	11.799324232335358
13	19.029790710051117	39.883906580689874	28.554896840229215	12.531405869029792
14	13.46089582534005	40.02623859796775	32.85332376202087	13.659541814671336
15	12.11431117491986	20.148644132826714	54.61031969008751	13.126725002165923
16	14.091488545366785	15.142270133792096	52.96916964738789	17.79707167345322
17	15.356386994566629	15.864450412762851	34.03839251457356	34.74077007809696
18	20.185155389432776	20.040348032723987	40.5516293921804	19.222867185662835
19	27.300518583610778	21.49089693924279	31.738802183249376	19.46978229389705
20	29.676225602435736	20.164733839127692	30.65707885193757	19.501961706499003
21	20.189487233436886	26.716957312771516	33.93876010247905	19.154795351312547
22	21.362798123692713	21.627659442801097	30.830971446959666	26.17857098654653
23	17.517358317759324	28.25723727366115	30.42749111971979	23.797913288859736
24	17.66340334418357	20.232805673477973	45.80182432516059	16.301966657177864
25	15.471490278104385	21.78422466180675	43.15939948265406	19.584885577434804
26	14.835328044358082	31.60327734940654	34.039630184289024	19.521764421946358
27	15.809992945282621	31.92754681485699	34.80017822443903	17.462282015421366
28	13.83776625369754	25.057242224340015	44.977536294664404	16.127455227298043
29	14.463408294862434	21.529883535279776	43.591965048207236	20.414743121650556
30	17.003725385843534	28.567273537383812	36.692575219376955	17.736425857395695
31	28.42122851095957	23.654343601866408	30.552495760981223	17.371932126192803
32	29.80061140883944	23.02870156070151	30.89099842815946	16.27968860229959
33	27.180464621211183	24.766389841206976	29.581543869203063	18.471601668378774
34	18.472839338094236	25.34438159832667	31.91702662227558	24.265752441303515
35	19.23586271767516	24.577645209599368	35.23831330371177	20.9481787690137
36	30.0568090399396	22.762602571877668	28.864933103951877	18.31565528423085
37	19.630679356906818	31.243115462207754	31.042612968303278	18.08359221258215
38	20.055818904167232	31.017859573994084	27.11363045657636	21.812691065262324
39	20.191962572867805	30.275257744718242	29.391561567879997	20.141218114533956
40	24.577645209599368	24.54608463185514	27.33888634479003	23.53738381375546
41	17.831107590628363	22.505786105919775	30.034530985061323	29.62857531839053
42	21.802789707538647	22.88822604799683	26.77017711053628	28.53880713392824
43	21.826924266990112	24.58754656732304	27.879748010445933	25.705781155240913
44	19.1640778741785	28.357488520613387	30.206567075510225	22.271866529697885
45	16.50246915108234	36.57994727527012	24.953896803099124	21.96368677054841
46	21.198806886394298	31.30438011312301	27.19222248350805	20.304590516974642
47	21.238412317289008	25.32457888287932	29.276458284342237	24.160550515489437
48	22.10168694382217	22.080027723801628	33.70731586568808	22.11096946668812
49	20.740250256816466	22.56457541740411	34.31872470512519	22.37644962065423
50	18.22097355099818	31.261680507939648	29.291929155785486	21.225416785276682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	123.0
2	158.0
3	1825.5
4	3493.0
5	2581.5
6	1670.0
7	1788.5
8	1907.0
9	2044.0
10	2181.0
11	2210.0
12	2239.0
13	2102.5
14	1966.0
15	1779.0
16	1592.0
17	1458.0
18	1324.0
19	1252.5
20	1181.0
21	1077.5
22	974.0
23	928.0
24	882.0
25	868.0
26	854.0
27	968.5
28	1083.0
29	1276.0
30	1469.0
31	2048.0
32	2627.0
33	2677.5
34	2728.0
35	3003.0
36	3278.0
37	3648.5
38	4019.0
39	5408.5
40	6798.0
41	9511.0
42	12224.0
43	14722.0
44	17220.0
45	17092.5
46	16965.0
47	15439.0
48	13913.0
49	12885.0
50	11857.0
51	11197.5
52	10538.0
53	9374.0
54	8210.0
55	7205.5
56	6201.0
57	5505.0
58	4809.0
59	4499.5
60	4190.0
61	3726.5
62	3263.0
63	3071.5
64	2880.0
65	2546.0
66	2212.0
67	1927.5
68	1643.0
69	1348.0
70	1053.0
71	889.0
72	725.0
73	628.0
74	531.0
75	439.5
76	348.0
77	238.5
78	129.0
79	101.5
80	74.0
81	56.5
82	39.0
83	32.0
84	25.0
85	18.5
86	12.0
87	10.0
88	8.0
89	10.0
90	12.0
91	6.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	161594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.793519561369855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91028586013272	28.284466007401264
2	8.523162327718223	6.612869289701351
3	3.9018631955079126	4.541010186021758
4	2.4486345074017355	3.7996460264613785
5	1.7563169984686064	3.4066858918029137
6	1.427705461970393	3.3231431860093816
7	1.137378764675855	3.0886047749297623
8	0.9698825931597753	3.010012747998069
9	0.8965033180193976	3.130066710397663
>10	5.999553343542624	38.72854190130822
>50	0.025523226135783564	0.5835612708392638
>100	0.0015952016334864728	0.14233201727786923
>500	0.0	0.0
>1k	0.0015952016334864728	1.3490599898511084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2180	1.3490599898511084	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	230	0.14233201727786923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02165922002054532	0.0
2	0.0	0.0	0.0	0.08478037550899167	0.0
3	0.0	0.0	0.0	0.12438580640370311	0.0
4	0.0	0.0	0.0	0.20607200762404546	0.0
5	0.0	0.0	0.0	0.37748926321521836	0.0
6	0.0	0.0	0.0	0.5637585553919081	0.0
7	0.0	0.0	0.0	0.7073282423852371	0.0
8	0.0	0.0	0.0	1.11018973476738	0.0
9	0.0	0.0	0.0	1.3069792195254775	0.0
10	0.0	0.0	0.0	1.6293921804027378	0.0
11	0.0	0.0	0.0	1.8329888485958636	0.0
12	0.0	0.0	0.0	2.026065324207582	0.0
13	0.0	0.0	0.0	2.1058950208547347	0.0
14	0.0	0.0	0.0	2.1306484151639293	0.0
15	0.0	0.0	0.0	2.164684332339072	0.0
16	0.0	0.0	0.0	2.2500835427057937	0.0
17	0.0	0.0	0.0	2.363949156528089	0.0
18	0.0	0.0	0.0	2.5236085498223946	0.0
19	0.0	0.0	0.0	2.5780660173026226	0.0
20	0.0	0.0	0.0	2.6492320259415574	0.0
21	6.188348577298662E-4	0.0	0.0	2.733393566592819	0.0
22	6.188348577298662E-4	0.0	0.0	2.8082725843781327	0.0
23	6.188348577298662E-4	0.0	0.0	2.890577620456205	0.0
24	6.188348577298662E-4	0.0	0.0	2.968550812530168	0.0
25	6.188348577298662E-4	0.0	0.0	3.019295270864017	0.0
26	6.188348577298662E-4	0.0	0.0	3.0774657474906246	0.0
27	6.188348577298662E-4	0.0	0.0	3.141205737836801	0.0
28	6.188348577298662E-4	0.0	0.0	3.19071252645519	0.0
29	6.188348577298662E-4	0.0	0.0	3.250739507654987	0.0
30	6.188348577298662E-4	0.0	0.0	3.3466589106031166	0.0
31	6.188348577298662E-4	0.0	0.0	3.4252509375348095	0.0
32	6.188348577298662E-4	0.0	0.0	3.4889909278809856	0.0
33	6.188348577298662E-4	0.0	0.0	3.5694394593858685	0.0
34	6.188348577298662E-4	0.0	0.0	3.6344171194475043	0.0
35	0.0012376697154597323	0.0	0.0	3.7383813755461217	0.0
36	0.0012376697154597323	0.0	0.0	3.821305246481924	0.0
37	0.0012376697154597323	0.0	0.0	3.899897273413617	0.0
38	0.0012376697154597323	0.0	0.0	3.9815834746339593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	25	4.425818E-5	44.0	3
CTGCTCA	40	8.236384E-9	44.0	44
TCGTTTG	20	7.8363833E-4	44.0	1
ATGATTG	25	4.425818E-5	44.0	1
AATTTCG	20	7.8363833E-4	44.0	37
CACGGGA	30	2.514178E-6	44.0	4
TATGGGC	25	4.425818E-5	44.0	4
GCAGTGT	55	1.8189894E-12	44.0	43
TTCACCC	20	7.8363833E-4	44.0	44
TGAACGG	25	4.425818E-5	44.0	2
ATTCGCC	20	7.8363833E-4	44.0	14
CCTAAGT	20	7.8363833E-4	44.0	20
ACGGGAG	50	2.7284841E-11	44.0	5
GTTACAC	20	7.8363833E-4	44.0	33
GTTGTAG	20	7.8363833E-4	44.0	1
GTTCGGG	25	4.425818E-5	44.0	3
CTACGGG	30	2.514178E-6	44.0	3
TGATTCC	20	7.8363833E-4	44.0	23
CAAGGGC	25	4.425818E-5	44.0	4
TGCGAAG	20	7.8363833E-4	44.0	1
>>END_MODULE