FastQCFastQC Report
Sat 18 Jun 2016
SRR3549144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159228
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22811.4325369909814856No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA1720.10802120230110283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG302.5139452E-644.03
TCGCCCA207.836038E-444.018
CTTAACG254.4255263E-544.01
CTGCTAG207.836038E-444.01
CTAGAAA207.836038E-444.041
TCGTTTC302.5139452E-644.041
TCCGCAA302.5139452E-644.032
AGTTTAG207.836038E-444.01
ATAGGGC254.4255263E-544.04
CATGCAT207.836038E-444.025
GGCACTA207.836038E-444.037
ACACCAT207.836038E-444.023
TGTGGGC207.836038E-444.04
ATAGCGG351.435601E-744.02
TAAGTTC302.5139452E-644.022
GTTAGAG207.836038E-444.01
CACTGGG408.234565E-944.03
TACGGGC207.836038E-444.04
TACGGGA254.4255263E-544.04
TGCGGGA302.5139452E-644.04
Produced by FastQC (version 0.11.5)