##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136467
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.979335663567017	28.0	25.0	30.0	16.0	33.0
2	25.938849685271897	30.0	25.0	31.0	10.0	33.0
3	26.263800039570008	30.0	25.0	31.0	16.0	33.0
4	28.06266716495563	32.0	19.0	35.0	10.0	37.0
5	31.17721500436003	33.0	30.0	35.0	19.0	37.0
6	29.65300768684004	33.0	28.0	35.0	17.0	37.0
7	29.544571215019015	32.0	28.0	35.0	17.0	35.0
8	32.38610066902621	33.0	32.0	35.0	28.0	35.0
9	26.890471689126308	30.0	23.0	34.0	10.0	35.0
10	31.874284625587137	34.0	31.0	37.0	18.0	37.0
11	33.72098749148146	35.0	32.0	37.0	27.0	39.0
12	32.86567448540673	35.0	31.0	37.0	23.0	39.0
13	32.328445704822414	35.0	30.0	37.0	21.0	39.0
14	31.996270160551635	34.0	29.0	38.0	20.0	40.0
15	31.398015637480125	33.0	27.0	38.0	17.0	40.0
16	30.19021448408773	33.0	26.0	37.0	17.0	40.0
17	30.07307993873977	32.0	25.0	37.0	17.0	40.0
18	31.073541588809015	33.0	27.0	37.0	18.0	39.0
19	30.740853100016853	34.0	27.0	37.0	18.0	39.0
20	31.489598217884176	34.0	29.0	36.0	19.0	39.0
21	32.230378040112264	34.0	30.0	37.0	23.0	39.0
22	32.5856727267398	34.0	30.0	37.0	24.0	39.0
23	34.00763554558978	35.0	33.0	38.0	28.0	39.0
24	33.6850887027633	35.0	33.0	38.0	26.0	40.0
25	32.4298694922582	34.0	31.0	37.0	23.0	39.0
26	26.256904599646802	33.0	20.0	35.0	0.0	38.0
27	29.273472707687574	33.0	23.0	36.0	12.0	39.0
28	30.839162581429942	34.0	27.0	36.0	18.0	39.0
29	32.23810884682744	34.0	30.0	37.0	23.0	39.0
30	31.904057391164166	34.0	30.0	37.0	22.0	39.0
31	30.873998842210938	33.0	29.0	36.0	18.0	38.0
32	31.01211281848359	34.0	29.0	36.0	18.0	38.0
33	30.788989279459503	33.0	29.0	36.0	18.0	38.0
34	30.90730359720665	34.0	30.0	37.0	16.0	39.0
35	30.100815581789004	34.0	28.0	36.0	12.0	39.0
36	29.745652795181254	33.0	27.0	36.0	10.0	39.0
37	29.733371437783493	33.0	27.0	36.0	10.0	39.0
38	29.51158888229389	33.0	27.0	36.0	10.0	38.0
39	29.42761986414298	33.0	26.0	36.0	10.0	38.0
40	29.429451808862215	33.0	26.0	36.0	10.0	38.0
41	28.902782357639577	33.0	24.0	35.0	10.0	38.0
42	29.082657345731935	33.0	25.0	35.0	10.0	38.0
43	28.644412202217385	33.0	24.0	35.0	10.0	38.0
44	28.332541933214625	32.0	23.0	35.0	9.0	38.0
45	27.762843764426563	31.0	23.0	34.0	9.0	37.0
46	27.375922384166135	31.0	23.0	34.0	8.0	38.0
47	27.58578997120183	31.0	23.0	34.0	8.0	37.0
48	27.754006463100968	31.0	23.0	34.0	8.0	37.0
49	27.60482754072413	31.0	24.0	34.0	8.0	37.0
50	29.345680640740984	33.0	26.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	8.0
13	25.0
14	55.0
15	116.0
16	203.0
17	432.0
18	717.0
19	1095.0
20	1520.0
21	2217.0
22	2867.0
23	3745.0
24	4810.0
25	6048.0
26	7034.0
27	7911.0
28	8880.0
29	10042.0
30	12443.0
31	14251.0
32	14898.0
33	13746.0
34	10902.0
35	7835.0
36	3848.0
37	792.0
38	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.99261359889204	24.693882037415637	27.58688913803337	23.72661522565895
2	36.070258743872145	18.217591065971995	32.28472817604256	13.427422014113302
3	28.748342090029087	14.715645540680164	42.05851964211128	14.477492727179463
4	25.39148658650077	25.227344339657204	35.22023639414657	14.160932679695456
5	19.40322568826163	29.527284984648304	38.104450160111966	12.965039166978098
6	17.81456322774004	35.3755853063378	35.96620428381953	10.843647182102632
7	80.20400536393414	3.32974272168363	14.969186689822447	1.4970652245597837
8	82.7621329698755	1.8312119413484578	14.11696600643379	1.289689082342251
9	71.17618178753838	2.5998959455399473	14.071533777396732	12.15238848952494
10	54.216770354737776	18.553203338536058	19.28964511566899	7.940381191057179
11	49.15327515076905	15.431569536957653	26.218060043820117	9.197095268453179
12	43.319630386833445	18.229315512175106	27.99797753302996	10.453076567961485
13	18.40957887254794	41.66062124909319	28.162852557761216	11.766947320597653
14	12.301142400726915	41.33746620061993	33.07979218419105	13.281599214462103
15	10.547604915474071	18.177288282148798	55.886771160793444	15.38833564158368
16	12.288685176636111	13.063231403929155	53.98081587490016	20.667267544534575
17	14.329471593865184	14.772802215920333	34.586383521290855	36.31134266892362
18	19.515340705078884	19.45598569617563	42.09808964804678	18.930583950698704
19	28.688254303238146	20.22613525614251	32.32063429253959	18.764976148079757
20	30.528259579238938	19.565169601442108	30.95400353198942	18.95256728732954
21	19.724182403071804	26.88049125429591	34.61569463679864	18.779631705833644
22	20.857057017447442	21.310646529930313	30.561967362072885	27.27032909054936
23	16.669231389273598	28.495533718774503	31.074911883459006	23.760323008492897
24	16.361464676441923	19.39003568628313	48.6439945188214	15.604505118453545
25	14.734697765760222	20.31626693632893	45.674778517883446	19.274256780027407
26	11.60353785164179	43.023588120204884	28.365099254764885	17.007774773388437
27	14.483354950281022	33.729766170576035	35.040705811661425	16.746173067481514
28	12.930598606256458	25.186308777946316	46.667692555709436	15.215400060087786
29	13.596693706170724	21.225644294957753	45.02846842093693	20.149193577934593
30	16.481640250023815	29.040720467219185	37.19287446782006	17.284764814936945
31	29.5009049806913	23.046597345878492	30.904174635626198	16.54832303780401
32	30.863139073915306	21.88514439388277	31.405394710809208	15.846321821392717
33	28.326994804604777	23.90248191870562	29.800611136758338	17.969912139931267
34	17.976507140920518	24.633794250624693	31.921270343746105	25.468428264708688
35	18.939377285351036	23.827738574160787	35.99185150988884	21.24103263059934
36	31.598115295272848	21.92617995559366	28.18923256171822	18.286472187415274
37	18.962093399869566	32.573442663794175	30.42054122974785	18.043922706588404
38	19.351198458235324	32.61154711395429	26.566129540474986	21.4711248873354
39	19.858280756519893	30.97818520228334	28.911751558984953	20.251782482211816
40	25.164325441315484	23.640147434911004	26.99993404999011	24.195593073783407
41	16.93742809616977	21.660181582360572	29.769101687587472	31.633288633882184
42	21.029992598943334	21.864626613027323	26.072970022056612	31.032410765972724
43	21.266679856668645	23.42544351381653	27.87120695845882	27.43666967105601
44	18.259359405570578	27.81185194955557	30.847017960386026	23.081770684487825
45	15.486527878534737	38.681146357727506	23.987484153678178	21.844841610059575
46	20.148460800046898	31.600313628935933	26.85924069555277	21.3919848754644
47	20.47308140429554	24.481376449984246	28.996753793957513	26.048788351762695
48	21.117193167578975	21.458667663244594	34.919797460191845	22.50434170898459
49	20.270101929404177	22.431796698102836	34.4889240622275	22.809177310265486
50	18.029267148834517	32.5177515443294	28.513853165966864	20.93912814086922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	75.5
2	100.0
3	1031.0
4	1962.0
5	1627.5
6	1293.0
7	1369.0
8	1445.0
9	1579.0
10	1713.0
11	1823.0
12	1933.0
13	1902.0
14	1871.0
15	1831.0
16	1791.0
17	1695.0
18	1599.0
19	1460.5
20	1322.0
21	1231.5
22	1141.0
23	1049.0
24	957.0
25	953.5
26	950.0
27	985.0
28	1020.0
29	1203.0
30	1386.0
31	1761.5
32	2137.0
33	2209.5
34	2282.0
35	2522.5
36	2763.0
37	3018.0
38	3273.0
39	4361.5
40	5450.0
41	7839.5
42	10229.0
43	12302.0
44	14375.0
45	14197.5
46	14020.0
47	12781.5
48	11543.0
49	10787.5
50	10032.0
51	9430.0
52	8828.0
53	7921.5
54	7015.0
55	6106.5
56	5198.0
57	4695.5
58	4193.0
59	3811.0
60	3429.0
61	3133.0
62	2837.0
63	2609.0
64	2381.0
65	2137.0
66	1893.0
67	1653.0
68	1413.0
69	1185.5
70	958.0
71	784.0
72	610.0
73	533.0
74	456.0
75	392.5
76	329.0
77	220.5
78	112.0
79	98.0
80	84.0
81	61.5
82	39.0
83	29.5
84	20.0
85	18.0
86	16.0
87	12.0
88	8.0
89	7.5
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	136467.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.03405951622003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72265522833081	45.23218067371599
2	7.448867500130774	8.347805696615298
3	3.2641104775853953	5.487040823056123
4	1.9799131662917822	4.437702887877656
5	1.3299680912277032	3.726175558926334
6	1.0030339488413456	3.372243839169909
7	0.7820264685881676	3.0674082378890133
8	0.5740963540304441	2.5735159415829467
9	0.5126327352618089	2.585240387786058
>10	2.3735418737249567	19.962335216572505
>50	0.006538682847727154	0.23961836927608873
>100	0.0013077365695454309	0.16047835740508692
>500	0.0	0.0
>1k	0.0013077365695454309	0.8082540101269905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1103	0.8082540101269905	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	219	0.16047835740508692	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026380003957000592	0.0
2	0.0	0.0	0.0	0.08060556764639071	0.0
3	0.0	0.0	0.0	0.1201755735818916	0.0
4	0.0	0.0	0.0	0.17586669304667063	0.0
5	0.0	0.0	0.0	0.2821194867623675	0.0
6	0.0	0.0	0.0	0.4162178402104538	0.0
7	0.0	0.0	0.0	0.4894956289798999	0.0
8	0.0	0.0	0.0	0.751830112774517	0.0
9	0.0	0.0	0.0	0.9049806913026592	0.0
10	0.0	0.0	0.0	1.1372712817018034	0.0
11	0.0	0.0	0.0	1.2677057457114174	0.0
12	0.0	0.0	0.0	1.4032696549348926	0.0
13	0.0	0.0	0.0	1.4552968849611994	0.0
14	0.0	0.0	0.0	1.4662885532766163	0.0
15	0.0	0.0	0.0	1.4868063341320612	0.0
16	0.0	0.0	0.0	1.5256435621798676	0.0
17	0.0	0.0	0.0	1.591593572072369	0.0
18	0.0	0.0	0.0	1.688320253248038	0.0
19	0.0	0.0	0.0	1.724959147632761	0.0
20	0.0	0.0	0.0	1.7733224882205953	0.0
21	0.0	0.0	0.0	1.8136252720437909	0.0
22	0.0	0.0	0.0	1.8466002769900416	0.0
23	0.0	0.0	0.0	1.9008258406794316	0.0
24	0.0	0.0	0.0	1.9381975129518492	0.0
25	0.0	0.0	0.0	1.9741036294488779	0.0
26	0.0	0.0	0.0	2.0092769680582117	0.0
27	0.0	0.0	0.0	2.0400536393413793	0.0
28	0.0	0.0	0.0	2.075959755838408	0.0
29	0.0	0.0	0.0	2.128719763752409	0.0
30	0.0	0.0	0.0	2.1997992188587716	0.0
31	0.0	7.327778876944609E-4	0.0	2.249628115221995	0.0
32	0.0	7.327778876944609E-4	0.0	2.300189789472913	0.0
33	0.0	7.327778876944609E-4	0.0	2.346354796397664	0.0
34	0.0	7.327778876944609E-4	0.0	2.402045915862443	0.0
35	0.0	7.327778876944609E-4	0.0	2.4804531498457503	0.0
36	0.0	7.327778876944609E-4	0.0	2.5346787135351403	0.0
37	0.0	7.327778876944609E-4	0.0	2.590369832999919	0.0
38	0.0	7.327778876944609E-4	0.0	2.646793730352393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	60	0.0	44.000004	9
CGTAGGG	60	0.0	44.000004	3
CAGTGTC	30	2.5112877E-6	44.000004	44
TAAGGCG	30	2.5112877E-6	44.000004	4
CGATGGT	30	2.5112877E-6	44.000004	10
GCGTAGG	30	2.5112877E-6	44.000004	2
TTAGGCG	35	1.4335819E-7	44.0	4
AGTTTAG	20	7.8320864E-4	44.0	1
ATCTAGG	20	7.8320864E-4	44.0	2
AGATAAT	20	7.8320864E-4	44.0	19
CGACGGG	45	4.7293724E-10	44.0	3
GCGGTCT	20	7.8320864E-4	44.0	36
TTAGCGG	55	1.8189894E-12	44.0	2
TCCGAGT	20	7.8320864E-4	44.0	18
TGGACAG	20	7.8320864E-4	44.0	1
ACCTGGG	20	7.8320864E-4	44.0	2
GTTAGGC	20	7.8320864E-4	44.0	3
TCCTCAA	70	0.0	44.0	21
TAACGGG	25	4.4221808E-5	44.0	3
ACGGGAT	35	1.4335819E-7	44.0	5
>>END_MODULE