FastQCFastQC Report
Sat 18 Jun 2016
SRR3549142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498236
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146810.97988567889171No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36950.24662336240752458No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG600.044.0000041
TTCGTCA302.5282334E-644.00000433
CACGACC302.5282334E-644.00000427
ACGGGTA600.044.0000045
AATGCGC302.5282334E-644.0000044
CGTAGAT302.5282334E-644.00000436
TACTACG302.5282334E-644.0000041
TCGACTC302.5282334E-644.00000427
TCGAACA302.5282334E-644.00000423
TACGTTA302.5282334E-644.00000427
TGATCGA254.443489E-544.010
CGGGTAT1100.044.06
CGGGTAC408.314601E-944.06
CTATGCG800.044.01
ATCTCGT254.443489E-544.019
CGTATTA254.443489E-544.036
CCCTCGA502.7284841E-1144.037
TACTTCG254.443489E-544.029
CGTTGCC408.314601E-944.024
ACTTCGC551.8189894E-1244.035
Produced by FastQC (version 0.11.5)