##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1498236
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53790390832953	31.0	31.0	33.0	30.0	34.0
2	31.928471882934332	31.0	31.0	34.0	30.0	34.0
3	31.96619824914099	33.0	31.0	34.0	30.0	34.0
4	35.725237546020786	37.0	35.0	37.0	33.0	37.0
5	35.66259120725974	37.0	35.0	37.0	33.0	37.0
6	35.71415851708276	37.0	35.0	37.0	33.0	37.0
7	36.00988896275353	37.0	35.0	37.0	35.0	37.0
8	36.008463286157856	37.0	35.0	37.0	35.0	37.0
9	37.83422037649609	39.0	38.0	39.0	35.0	39.0
10	37.34229854308667	39.0	37.0	39.0	34.0	39.0
11	36.91381197621737	39.0	35.0	39.0	33.0	39.0
12	35.644690823074605	37.0	35.0	39.0	32.0	39.0
13	35.115911645428355	37.0	35.0	39.0	30.0	39.0
14	35.97041721063971	37.0	35.0	41.0	31.0	41.0
15	36.341620412271496	37.0	35.0	41.0	31.0	41.0
16	36.609665633451606	37.0	35.0	41.0	32.0	41.0
17	36.5698808465422	37.0	35.0	41.0	32.0	41.0
18	36.47223267896379	36.0	35.0	40.0	32.0	41.0
19	36.29879605082243	36.0	35.0	40.0	32.0	41.0
20	36.02719464757221	35.0	35.0	40.0	32.0	41.0
21	35.85375201236654	35.0	35.0	40.0	31.0	41.0
22	35.705182628103984	35.0	35.0	40.0	31.0	41.0
23	35.717230796750314	35.0	35.0	40.0	31.0	41.0
24	35.74134849249384	35.0	35.0	40.0	31.0	41.0
25	35.702894604054364	35.0	35.0	40.0	31.0	41.0
26	35.47370440971916	35.0	35.0	40.0	31.0	41.0
27	35.44690422603649	35.0	34.0	40.0	31.0	41.0
28	35.48928606708156	35.0	35.0	40.0	31.0	41.0
29	35.50094511145107	35.0	35.0	40.0	31.0	41.0
30	35.405895332911506	36.0	35.0	40.0	30.0	41.0
31	35.09726905507544	35.0	34.0	40.0	30.0	41.0
32	34.76650207310464	35.0	34.0	40.0	29.0	41.0
33	34.43483736874565	35.0	34.0	40.0	27.0	41.0
34	34.16121959424283	35.0	34.0	40.0	24.0	41.0
35	33.916639301151484	35.0	33.0	40.0	23.0	41.0
36	33.679421666546524	35.0	33.0	40.0	22.0	41.0
37	33.58039521143532	35.0	33.0	40.0	22.0	41.0
38	33.552582503691006	35.0	33.0	40.0	23.0	41.0
39	33.50987961843128	35.0	33.0	40.0	22.0	41.0
40	33.326881078815354	35.0	33.0	40.0	21.0	41.0
41	33.336599173961915	35.0	33.0	39.0	21.0	41.0
42	33.29098419741616	35.0	33.0	39.0	21.0	41.0
43	33.16470636134761	35.0	33.0	39.0	20.0	41.0
44	33.01449037401317	35.0	33.0	39.0	18.0	41.0
45	32.910157011311966	35.0	33.0	39.0	20.0	41.0
46	32.89328250022026	35.0	33.0	39.0	20.0	41.0
47	32.849059827690695	35.0	33.0	39.0	20.0	41.0
48	32.79229040017727	35.0	33.0	39.0	18.0	41.0
49	32.765809258354494	35.0	33.0	38.0	18.0	41.0
50	32.52952672342675	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	7.0
14	12.0
15	31.0
16	107.0
17	321.0
18	693.0
19	1464.0
20	2589.0
21	4278.0
22	6733.0
23	10058.0
24	14926.0
25	23099.0
26	33315.0
27	40202.0
28	40508.0
29	38986.0
30	42718.0
31	49478.0
32	61272.0
33	82386.0
34	166369.0
35	309261.0
36	77602.0
37	94026.0
38	140535.0
39	257256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.336718647796474	22.393468051762206	29.01418735099143	17.255625949449886
2	33.729465851841766	24.09847313774332	29.697457543404376	12.474603467010539
3	27.506547700095314	24.341425516407295	35.768196732690974	12.383830050806415
4	24.047680071764397	25.610784949767595	36.25403474485995	14.087500233608056
5	21.833075697019698	31.584343187588605	34.09476210690438	12.487819008487316
6	20.027085185511496	38.41223946027195	31.16271401835225	10.39796133586431
7	84.74098873608698	3.7191737483280334	10.336422299290632	1.203415216294362
8	86.63595054450701	2.1139526750124813	9.965052234761412	1.285044545719099
9	84.04076527329472	3.3464020354603683	10.851628181408003	1.7612045098369014
10	59.632928323708676	17.89451061114537	14.98028348003919	7.492277585106752
11	54.05056346263205	15.278701085810246	21.073115316946062	9.597620134611637
12	48.67477486857878	18.40651272563201	22.87830488654658	10.04040751924263
13	19.93477663065098	45.863802498404795	22.8096908631217	11.391730007822533
14	12.440429945616044	46.112428215581524	28.562389369898998	12.884752468903429
15	10.79255871571635	20.116857424331013	56.878956319298155	12.211627540654476
16	12.42340993007777	14.107123310346301	55.42217647953994	18.047290280035988
17	13.682690844433054	15.83288614076821	30.35563155604324	40.1287914587555
18	20.84751667961523	21.21414783785732	38.01276968381483	19.92556579871262
19	28.66804695655424	23.05371116432925	27.544392205233354	20.733849673883153
20	32.12638062361337	20.50184350129085	27.479849636505865	19.891926238589917
21	20.509852920367685	27.992585947741212	30.772788799628366	20.72477233226274
22	21.68163093130855	22.25764165325089	28.558785131314423	27.50194228412613
23	17.868746979781555	28.818957760993598	27.5771640782894	25.735131180935443
24	19.1589976479006	20.13728144297694	45.001855515419464	15.701865393702994
25	14.535894211592835	22.291414703691544	41.55466828990893	21.618022794806695
26	13.34422614327783	35.19952797823574	31.183872233746886	20.272373644739545
27	14.631006063130242	35.671282761861285	32.446356915732906	17.251354259275576
28	11.965671629836688	26.39403939032302	45.84384569587168	15.796443283968614
29	12.599216678814285	21.351976591137845	44.32118838420649	21.727618345841375
30	16.887926868664216	29.877736217792123	35.29303794595778	17.94129896758588
31	30.500869021969834	24.845551702135044	26.30920629326755	18.34437298262757
32	30.93351114243684	25.09364345803999	28.387517053388116	15.585328346135055
33	28.68339834311817	27.37946491740954	26.79898226981597	17.138154469656318
34	17.504718882739432	26.99554676299328	30.61854073724033	24.881193617026955
35	17.936626806457728	25.02489594429716	34.715024869246236	22.32345237999888
36	31.53194823779431	23.203954517178868	28.129880739749947	17.13421650527687
37	19.264855470032757	31.89350676395441	30.669867764491045	18.17177000152179
38	18.752920100705094	33.048264759357004	25.560592590219432	22.63822254971847
39	19.3065044492323	31.799062364006737	29.038549334016807	19.85588385274416
40	24.189914005537176	25.516407294978894	25.820498239262708	24.47318046022122
41	15.253805141513086	23.49976906174995	29.972047127421845	31.274378669315116
42	22.413424854295318	23.057715873867668	24.852359708350352	29.676499563486658
43	23.31001257478795	24.468441553934092	25.73186066814574	26.48968520313222
44	19.241494664391993	30.2064561257372	28.531152635499346	22.02089657437146
45	15.96217151370011	40.012855117618315	22.017893042217647	22.00708032646392
46	22.136365699395824	33.25390659415473	26.298326832354853	18.311400874094602
47	21.04454838890535	26.180788607402306	28.36168667686533	24.412976326827014
48	22.877503944638896	21.546138258592105	33.63949337754533	21.936864419223674
49	20.355337877343757	21.938800028833906	34.300670922338	23.405191171484333
50	17.55938316793883	32.589992497844136	28.389185682362456	21.461438651854582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	550.0
1	1058.0
2	1566.0
3	13295.0
4	25024.0
5	18523.0
6	12022.0
7	12558.0
8	13094.0
9	14113.0
10	15132.0
11	14856.0
12	14580.0
13	13732.0
14	12884.0
15	11546.5
16	10209.0
17	9144.5
18	8080.0
19	7381.5
20	6683.0
21	6370.0
22	6057.0
23	5511.5
24	4966.0
25	5212.5
26	5459.0
27	6970.0
28	8481.0
29	12325.0
30	16169.0
31	18396.5
32	20624.0
33	23395.5
34	26167.0
35	29672.0
36	33177.0
37	34050.5
38	34924.0
39	49934.0
40	64944.0
41	93925.0
42	122906.0
43	146874.5
44	170843.0
45	177052.5
46	183262.0
47	160812.0
48	138362.0
49	125798.5
50	113235.0
51	108559.0
52	103883.0
53	90576.0
54	77269.0
55	67865.0
56	58461.0
57	52258.0
58	46055.0
59	41682.0
60	37309.0
61	36139.5
62	34970.0
63	29879.0
64	24788.0
65	20439.5
66	16091.0
67	13297.5
68	10504.0
69	8718.5
70	6933.0
71	5953.0
72	4973.0
73	4069.0
74	3165.0
75	2679.5
76	2194.0
77	1738.5
78	1283.0
79	859.5
80	436.0
81	325.5
82	215.0
83	199.0
84	183.0
85	128.5
86	74.0
87	50.5
88	27.0
89	22.5
90	18.0
91	10.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1498236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.780887091099487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51256117294923	15.120973208044001
2	8.541008989657627	3.2081545095765116
3	2.809291963200693	1.5828298550041628
4	1.290749244533147	0.9696566329799597
5	0.7383330758834542	0.6933275066895672
6	0.49274549382904403	0.5552518490550802
7	0.33354342591703456	0.43849689954884324
8	0.28550877960124954	0.428968652256694
9	0.21985801493774945	0.3716215699157223
>10	2.2467392274644085	9.853131475183972
>50	0.9690667995311234	13.313082666030562
>100	1.551587615516428	51.26508712219544
>500	0.008285701220542802	0.9561392867090214
>1k	3.6024787915403485E-4	0.24999537676123929
>5k	0.0	0.0
>10k+	3.6024787915403485E-4	0.9932833900491891
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14681	0.97988567889171	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3695	0.24662336240752458	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06067134950702026	0.0
2	0.0	0.0	0.0	0.2494266590844166	0.0
3	0.0	0.0	0.0	0.336395601227043	0.0
4	0.0	0.0	0.0	0.4903099378202099	0.0
5	0.0	0.0	0.0	0.8120883492320302	0.0
6	0.0	0.0	0.0	1.0856100107059234	0.0
7	0.0	0.0	0.0	1.2540747919553394	0.0
8	0.0	0.0	0.0	1.646736562197144	0.0
9	0.0	0.0	0.0	1.7943101086878168	0.0
10	0.0	0.0	0.0	2.0703013410437343	0.0
11	0.0	0.0	0.0	2.3620444309174258	0.0
12	0.0	0.0	0.0	2.5939838583507537	0.0
13	0.0	0.0	0.0	2.6895629260009772	0.0
14	0.0	0.0	0.0	2.7228687603288133	0.0
15	0.0	0.0	0.0	2.7806033228409945	0.0
16	0.0	0.0	0.0	2.9195667438240704	0.0
17	0.0	0.0	0.0	3.0801555963145995	0.0
18	0.0	0.0	0.0	3.3491385869782864	0.0
19	0.0	0.0	0.0	3.4415806321567497	0.0
20	0.0	0.0	0.0	3.5439677060222823	0.0
21	0.0	0.0	0.0	3.6981490232513434	0.0
22	0.0	0.0	0.0	3.8369789539164723	0.0
23	0.0	0.0	0.0	4.009314954386358	0.0
24	0.0	0.0	0.0	4.131992556579871	0.0
25	0.0	0.0	0.0	4.225702759778833	0.0
26	0.0	0.0	0.0	4.3258872433982365	0.0
27	0.0	0.0	0.0	4.4189299950074625	0.0
28	0.0	0.0	0.0	4.515643730360237	0.0
29	0.0	0.0	0.0	4.62043362994882	0.0
30	0.0	0.0	0.0	4.771411179547147	0.0
31	0.0	6.674515897361964E-5	0.0	4.910241110212277	0.0
32	0.0	6.674515897361964E-5	0.0	5.02317391919564	0.0
33	0.0	6.674515897361964E-5	0.0	5.140044692558448	0.0
34	0.0	6.674515897361964E-5	0.0	5.2624553141160675	0.0
35	0.0	6.674515897361964E-5	0.0	5.439596966032053	0.0
36	0.0	6.674515897361964E-5	0.0	5.565545081015274	0.0
37	0.0	6.674515897361964E-5	0.0	5.689624331547233	0.0
38	0.0	6.674515897361964E-5	0.0	5.8064283597510675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	60	0.0	44.000004	1
TTCGTCA	30	2.5282334E-6	44.000004	33
CACGACC	30	2.5282334E-6	44.000004	27
ACGGGTA	60	0.0	44.000004	5
AATGCGC	30	2.5282334E-6	44.000004	4
CGTAGAT	30	2.5282334E-6	44.000004	36
TACTACG	30	2.5282334E-6	44.000004	1
TCGACTC	30	2.5282334E-6	44.000004	27
TCGAACA	30	2.5282334E-6	44.000004	23
TACGTTA	30	2.5282334E-6	44.000004	27
TGATCGA	25	4.443489E-5	44.0	10
CGGGTAT	110	0.0	44.0	6
CGGGTAC	40	8.314601E-9	44.0	6
CTATGCG	80	0.0	44.0	1
ATCTCGT	25	4.443489E-5	44.0	19
CGTATTA	25	4.443489E-5	44.0	36
CCCTCGA	50	2.7284841E-11	44.0	37
TACTTCG	25	4.443489E-5	44.0	29
CGTTGCC	40	8.314601E-9	44.0	24
ACTTCGC	55	1.8189894E-12	44.0	35
>>END_MODULE