FastQCFastQC Report
Sat 18 Jun 2016
SRR3549140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951642
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95030.9985898058303438No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18310.1924042864858844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG207.8557996E-444.01
CTTCGAA302.5272548E-644.038
CGGAATA207.8557996E-444.023
CTATGCG454.802132E-1044.01
TCGTTAG750.044.01
ACGCCGG454.802132E-1044.027
CGTATAT207.8557996E-444.038
GTCGACG254.4422603E-544.013
GTCGAAC254.4422603E-544.029
CAACGAC207.8557996E-444.012
ACGTTAG502.7284841E-1144.01
ATAATCG207.8557996E-444.030
TTCACGA207.8557996E-444.044
GGTCATA302.5272548E-644.08
TCGATTG207.8557996E-444.01
CGTTCTA207.8557996E-444.029
TAACGCC254.4422603E-544.012
TCGATAG800.044.01
TAGTTCG207.8557996E-444.01
TTATACG302.5272548E-644.01
Produced by FastQC (version 0.11.5)