##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	951642
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.504496438786855	31.0	31.0	33.0	30.0	34.0
2	31.890233932508234	31.0	31.0	34.0	30.0	34.0
3	31.887373613186472	31.0	31.0	34.0	30.0	34.0
4	35.67021527002802	37.0	35.0	37.0	33.0	37.0
5	35.61890605921134	37.0	35.0	37.0	33.0	37.0
6	35.68071291515086	37.0	35.0	37.0	33.0	37.0
7	36.006548681121686	37.0	35.0	37.0	35.0	37.0
8	36.01122480932956	37.0	35.0	37.0	35.0	37.0
9	37.85089245745774	39.0	38.0	39.0	35.0	39.0
10	37.34246386771496	39.0	37.0	39.0	34.0	39.0
11	36.92095556942632	39.0	37.0	39.0	33.0	39.0
12	35.7749626435151	37.0	35.0	39.0	32.0	39.0
13	35.27966294047552	37.0	35.0	39.0	30.0	39.0
14	36.19125889777879	38.0	35.0	41.0	31.0	41.0
15	36.56016022832115	38.0	35.0	41.0	32.0	41.0
16	36.82734053351996	38.0	35.0	41.0	32.0	41.0
17	36.758738054856764	38.0	35.0	41.0	32.0	41.0
18	36.63326755229382	37.0	35.0	40.0	32.0	41.0
19	36.440270605963164	37.0	35.0	40.0	32.0	41.0
20	36.14356344087378	36.0	35.0	40.0	32.0	41.0
21	35.94415126696804	35.0	35.0	40.0	31.0	41.0
22	35.824444486477056	35.0	35.0	40.0	31.0	41.0
23	35.83403317634152	35.0	35.0	40.0	31.0	41.0
24	35.83567560069858	35.0	35.0	40.0	31.0	41.0
25	35.79061243618924	35.0	35.0	40.0	31.0	41.0
26	35.53046523797815	35.0	35.0	40.0	31.0	41.0
27	35.51440457651091	35.0	34.0	40.0	30.0	41.0
28	35.5484751618781	35.0	35.0	40.0	31.0	41.0
29	35.57352765010371	36.0	35.0	40.0	31.0	41.0
30	35.47315271919482	36.0	35.0	40.0	30.0	41.0
31	35.14660555124721	35.0	34.0	40.0	30.0	41.0
32	34.76531195554631	35.0	34.0	40.0	28.0	41.0
33	34.40827117760671	35.0	34.0	40.0	25.0	41.0
34	34.09691039277375	35.0	34.0	40.0	23.0	41.0
35	33.78525958291038	35.0	33.0	40.0	22.0	41.0
36	33.53936144054172	35.0	33.0	40.0	21.0	41.0
37	33.421951742356896	35.0	33.0	40.0	20.0	41.0
38	33.40044365423132	35.0	33.0	40.0	20.0	41.0
39	33.34226421280271	35.0	33.0	40.0	19.0	41.0
40	33.146445827317415	35.0	33.0	40.0	18.0	41.0
41	33.1540411205054	35.0	33.0	40.0	18.0	41.0
42	33.11110270458849	35.0	33.0	40.0	18.0	41.0
43	32.98892125400098	35.0	33.0	40.0	18.0	41.0
44	32.85479728721515	35.0	33.0	39.0	18.0	41.0
45	32.731177270444135	35.0	32.0	39.0	18.0	41.0
46	32.72571408155588	35.0	32.0	39.0	18.0	41.0
47	32.687350915575394	35.0	32.0	39.0	18.0	41.0
48	32.635711748745855	35.0	32.0	39.0	18.0	41.0
49	32.63306789738158	35.0	32.0	39.0	18.0	41.0
50	32.429303246388876	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	3.0
14	9.0
15	25.0
16	71.0
17	205.0
18	479.0
19	1063.0
20	1813.0
21	2959.0
22	4604.0
23	6741.0
24	10383.0
25	15916.0
26	22784.0
27	27302.0
28	27223.0
29	25733.0
30	27351.0
31	30452.0
32	37462.0
33	49703.0
34	97588.0
35	181933.0
36	50615.0
37	62563.0
38	97222.0
39	169437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.16377587370041	22.22957792951551	27.057548952232036	18.549097244552048
2	34.513083701644106	24.009659094491415	29.057355602211754	12.419901601652722
3	26.21731701627293	23.875154732556993	37.871804733292564	12.035723517877521
4	22.795336901902186	26.593088577427228	36.87395049819155	13.737624022479041
5	20.720291874465396	30.730673929902213	35.90089550482219	12.648138690810198
6	18.472808051767366	40.33218374136492	31.57983779614603	9.61517041072168
7	84.77683834887489	2.9725463987507905	11.268943573318538	0.9816716790557795
8	86.64266604458399	1.5183230668675824	10.809422030553506	1.0295888579949182
9	84.37374558920267	2.8633666862118314	11.640091547031343	1.1227961775541644
10	55.83213014978322	21.39523055939103	15.42439278636294	7.348246504462813
11	49.90826382189941	15.189115234510457	24.512579310286853	10.39004163330328
12	44.05742915928469	18.55865966403332	26.394904806639474	10.989006370042516
13	18.415643697945235	44.02926730850467	26.27248482097259	11.282604172577502
14	11.463344408926886	44.54668877582116	32.23228903306075	11.757677782191202
15	10.321948800073976	19.83739683620521	57.46751404414685	12.373140319573958
16	12.69868290806837	14.723919288976317	55.20143079015008	17.375967012805233
17	14.09920957671057	14.978742005922395	32.37530499914884	38.54674341821819
18	21.094382131095518	19.654134643069558	39.64768263695802	19.60380058887691
19	27.644324231170962	21.655412434507934	29.950338467617023	20.749924866704074
20	30.87621185277657	20.34147294886102	29.640137782905757	19.14217741545665
21	19.915682578112357	26.231818267793983	33.40405320488167	20.448445949211994
22	21.18569798306506	21.03448565742159	30.454309498740074	27.325506860773274
23	16.35163223144838	28.562211419840654	29.16947759766803	25.91667875104293
24	18.425626443557555	20.556154520292296	45.87586508371846	15.142353952431693
25	14.628190012630801	21.048146256680557	43.561023998520454	20.76263973216819
26	14.634599986129238	33.84423974561862	32.22535365189851	19.295806616353627
27	14.850332372888122	33.883750401936865	34.39360599889454	16.87231122628047
28	13.015818973941881	26.33689980055525	45.33648157605486	15.310799649448006
29	13.24247984010794	20.76736840114245	44.80182673736552	21.18832502138409
30	16.093026579322895	29.141946236084575	36.567112422528645	18.197914762063885
31	29.943718331053066	23.330727311320853	28.468478692617598	18.25707566500848
32	30.122041692148937	24.488620720817284	30.01706524092043	15.37227234611335
33	28.221221846030335	25.93044443183466	27.349990857906647	18.498342864228352
34	18.48793979248499	26.514907917052845	29.708861105331625	25.28829118513054
35	18.692953862902225	25.33767950552834	34.01258036110218	21.956786270467255
36	31.749019063891676	22.357251991820455	28.9468098297469	16.94691911454097
37	19.686920081291074	32.153267720424275	30.38054226274166	17.779269935542988
38	19.47297408058913	32.955565223056574	25.23806221247066	22.333398483883645
39	19.584360505316077	31.245258195834147	29.878042373077268	19.292338925772505
40	24.710027510345277	25.608369533921373	25.655340979065656	24.026261976667694
41	16.40469840549282	23.48046849550566	29.464126215530626	30.650706883470885
42	22.583492531855466	24.217090040162162	25.25792262216254	27.941494805819833
43	23.637985713114805	24.46760441426503	26.18705353483768	25.707356337782482
44	18.183938918206636	30.64534772530006	29.662940475514954	21.50777288097835
45	16.174569848745644	39.7123077796062	23.742016430548464	20.3711059410997
46	23.16165112510797	31.667160549870644	27.134783878811568	18.036404446209815
47	21.075992862862293	27.19426002635445	27.859005802602237	23.870741308181017
48	23.837535543828455	22.21465635186341	32.88789271595831	21.059915388349822
49	21.001490056134557	21.83310530640724	34.364708577385194	22.80069606007301
50	18.886934372379528	32.97059188224143	28.215652524793988	19.926821220585055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	534.0
2	868.0
3	8795.5
4	16723.0
5	12815.0
6	8907.0
7	9291.5
8	9676.0
9	10385.5
10	11095.0
11	10848.5
12	10602.0
13	9987.5
14	9373.0
15	8530.5
16	7688.0
17	6845.0
18	6002.0
19	5517.0
20	5032.0
21	4889.5
22	4747.0
23	4453.0
24	4159.0
25	4388.5
26	4618.0
27	5464.5
28	6311.0
29	8003.0
30	9695.0
31	10870.5
32	12046.0
33	14409.5
34	16773.0
35	17613.0
36	18453.0
37	21817.5
38	25182.0
39	31371.5
40	37561.0
41	59656.0
42	81751.0
43	97677.5
44	113604.0
45	110570.0
46	107536.0
47	97345.0
48	87154.0
49	80987.5
50	74821.0
51	69749.0
52	64677.0
53	56362.0
54	48047.0
55	42640.0
56	37233.0
57	33691.5
58	30150.0
59	26752.5
60	23355.0
61	20854.0
62	18353.0
63	15947.5
64	13542.0
65	11786.0
66	10030.0
67	8369.5
68	6709.0
69	5223.5
70	3738.0
71	3112.0
72	2486.0
73	1934.0
74	1382.0
75	975.5
76	569.0
77	460.0
78	351.0
79	267.0
80	183.0
81	193.5
82	204.0
83	113.5
84	23.0
85	19.5
86	16.0
87	10.5
88	5.0
89	4.0
90	3.0
91	6.0
92	9.0
93	4.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	951642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.414111692276304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.29929326230003	16.59652853159719
2	6.417671406001235	2.6202212177287425
3	2.191734159420383	1.3422691779055507
4	1.215537179741322	0.9925644701341552
5	0.8049524972954546	0.8216195093383074
6	0.5918853614796492	0.7249688326961335
7	0.43615448495629	0.6232594459689464
8	0.3551188491716893	0.579954868081879
9	0.2851016369559515	0.5238086994422643
>10	3.770019039444741	18.466093066583646
>50	1.4721157099480044	21.710477804369198
>100	1.1588553149714316	33.73794398652392
>500	5.203661046122279E-4	0.0563009024362724
>1k	5.203661046122279E-4	0.19450368369965051
>5k	5.203661046122279E-4	1.0094858034941445
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9503	0.9985898058303438	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1831	0.1924042864858844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022907774141956744	0.0
2	0.0	0.0	0.0	0.08112294329170003	0.0
3	0.0	0.0	0.0	0.11243724005455834	0.0
4	0.0	0.0	0.0	0.17159814299915305	0.0
5	0.0	0.0	0.0	0.3003230206317081	0.0
6	0.0	0.0	0.0	0.41696352199671727	0.0
7	0.0	0.0	0.0	0.49945252521431377	0.0
8	0.0	0.0	0.0	0.7631020909123389	0.0
9	0.0	0.0	0.0	0.862193976306216	0.0
10	0.0	0.0	0.0	1.047452718564334	0.0
11	0.0	0.0	0.0	1.2189457800307257	0.0
12	0.0	0.0	0.0	1.3815069112124096	0.0
13	0.0	0.0	0.0	1.4445558308691715	0.0
14	0.0	0.0	0.0	1.4684093388059796	0.0
15	0.0	0.0	0.0	1.5034014892154823	0.0
16	0.0	0.0	0.0	1.5873616338917367	0.0
17	0.0	0.0	0.0	1.6902364544650195	0.0
18	0.0	0.0	0.0	1.8385064971911707	0.0
19	0.0	0.0	0.0	1.9056535966256218	0.0
20	0.0	0.0	0.0	1.9933966764812818	0.0
21	0.0	0.0	0.0	2.0941698663993393	0.0
22	0.0	0.0	0.0	2.2040851496676273	0.0
23	0.0	0.0	0.0	2.331969375038092	0.0
24	0.0	0.0	0.0	2.421603922483455	0.0
25	0.0	0.0	0.0	2.500520153587168	0.0
26	0.0	0.0	0.0	2.580382118485733	0.0
27	0.0	0.0	0.0	2.65709163740146	0.0
28	0.0	0.0	0.0	2.743258494265701	0.0
29	0.0	0.0	0.0	2.8332082863093473	0.0
30	0.0	0.0	0.0	2.965611017588547	0.0
31	0.0	0.0	0.0	3.081095622093182	0.0
32	0.0	0.0	0.0	3.177350306102505	0.0
33	0.0	0.0	0.0	3.2840080618551934	0.0
34	0.0	0.0	0.0	3.398126606433932	0.0
35	0.0	0.0	0.0	3.5448204261686644	0.0
36	0.0	0.0	0.0	3.6624066613285247	0.0
37	0.0	0.0	0.0	3.7898705605679446	0.0
38	0.0	0.0	0.0	3.9110295678416884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.8557996E-4	44.0	1
CTTCGAA	30	2.5272548E-6	44.0	38
CGGAATA	20	7.8557996E-4	44.0	23
CTATGCG	45	4.802132E-10	44.0	1
TCGTTAG	75	0.0	44.0	1
ACGCCGG	45	4.802132E-10	44.0	27
CGTATAT	20	7.8557996E-4	44.0	38
GTCGACG	25	4.4422603E-5	44.0	13
GTCGAAC	25	4.4422603E-5	44.0	29
CAACGAC	20	7.8557996E-4	44.0	12
ACGTTAG	50	2.7284841E-11	44.0	1
ATAATCG	20	7.8557996E-4	44.0	30
TTCACGA	20	7.8557996E-4	44.0	44
GGTCATA	30	2.5272548E-6	44.0	8
TCGATTG	20	7.8557996E-4	44.0	1
CGTTCTA	20	7.8557996E-4	44.0	29
TAACGCC	25	4.4422603E-5	44.0	12
TCGATAG	80	0.0	44.0	1
TAGTTCG	20	7.8557996E-4	44.0	1
TTATACG	30	2.5272548E-6	44.0	1
>>END_MODULE