##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413076
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.584158955356965	31.0	31.0	33.0	30.0	34.0
2	31.982054751478334	31.0	31.0	34.0	30.0	34.0
3	32.014274533004595	33.0	31.0	34.0	30.0	34.0
4	35.763672300711356	37.0	35.0	37.0	35.0	37.0
5	35.70732855133057	37.0	35.0	37.0	33.0	37.0
6	35.76857295715163	37.0	35.0	37.0	33.0	37.0
7	36.0523510412745	37.0	35.0	37.0	35.0	37.0
8	36.04866758758906	37.0	35.0	37.0	35.0	37.0
9	37.88587521124129	39.0	38.0	39.0	35.0	39.0
10	37.42609385482451	39.0	37.0	39.0	35.0	39.0
11	37.01365602416289	39.0	37.0	39.0	33.0	39.0
12	35.81803668026348	37.0	35.0	39.0	32.0	39.0
13	35.31679117046783	37.0	35.0	39.0	31.0	39.0
14	36.22184440185807	38.0	35.0	41.0	31.0	41.0
15	36.55199295720825	38.0	35.0	41.0	32.0	41.0
16	36.80365174979973	38.0	35.0	41.0	33.0	41.0
17	36.75692602521025	37.0	35.0	41.0	32.0	41.0
18	36.66965400304018	37.0	35.0	40.0	32.0	41.0
19	36.498523787821746	37.0	35.0	40.0	32.0	41.0
20	36.24147533465999	36.0	35.0	40.0	32.0	41.0
21	36.06157984425466	35.0	35.0	40.0	32.0	41.0
22	35.948904375985435	35.0	35.0	40.0	31.0	41.0
23	35.938323911806584	35.0	35.0	40.0	31.0	41.0
24	35.94973660298526	35.0	35.0	40.0	32.0	41.0
25	35.899535481460305	35.0	35.0	40.0	31.0	41.0
26	35.68137311793562	35.0	35.0	40.0	31.0	41.0
27	35.64334614698714	35.0	35.0	40.0	31.0	41.0
28	35.69021269910465	36.0	35.0	40.0	31.0	41.0
29	35.70343491786712	36.0	35.0	40.0	31.0	41.0
30	35.60915548774447	36.0	35.0	40.0	31.0	41.0
31	35.287294526267516	35.0	34.0	40.0	30.0	41.0
32	34.9588840232231	35.0	34.0	40.0	29.0	41.0
33	34.66349651398792	35.0	34.0	40.0	27.0	41.0
34	34.43157692862946	35.0	34.0	40.0	25.0	41.0
35	34.1687920536475	35.0	34.0	40.0	23.0	41.0
36	33.929838168647684	35.0	33.0	40.0	23.0	41.0
37	33.818776909380674	35.0	33.0	40.0	23.0	41.0
38	33.78621815104071	35.0	33.0	40.0	23.0	41.0
39	33.75935618466381	35.0	33.0	40.0	23.0	41.0
40	33.611324514746556	35.0	33.0	40.0	22.0	41.0
41	33.589238653830364	35.0	33.0	40.0	22.0	41.0
42	33.542776184720424	35.0	33.0	40.0	21.0	41.0
43	33.42894649686217	35.0	33.0	40.0	21.0	41.0
44	33.30519519119991	35.0	33.0	39.0	21.0	41.0
45	33.18731901185782	35.0	33.0	39.0	20.0	41.0
46	33.18205744064721	35.0	33.0	39.0	20.0	41.0
47	33.12414194282544	35.0	33.0	39.0	20.0	41.0
48	33.07661229827695	35.0	33.0	39.0	20.0	41.0
49	33.09265743668423	35.0	33.0	39.0	20.0	41.0
50	32.873949454947926	35.0	33.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	3.0
13	8.0
14	11.0
15	28.0
16	95.0
17	224.0
18	562.0
19	1202.0
20	2241.0
21	3682.0
22	5790.0
23	8807.0
24	13349.0
25	20686.0
26	29827.0
27	35372.0
28	35731.0
29	34831.0
30	38424.0
31	43694.0
32	55111.0
33	74109.0
34	149399.0
35	288013.0
36	74241.0
37	91297.0
38	143646.0
39	262691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.10646561119147	22.98071724380005	28.618489026775627	17.294328118232848
2	33.935895875381085	24.325655520297566	29.437199414610397	12.301249189710957
3	26.8017431475731	24.163314641250718	36.73878828881107	12.296153922365109
4	24.247245017253142	26.257540287995834	35.989783988971574	13.505430705779448
5	21.927836860862403	31.423433700664365	34.02187851184225	12.626850926630981
6	18.80564102709267	40.69540491806527	30.36262734629985	10.136326708542216
7	85.74223891708584	3.358913462545539	9.834078280290656	1.0647693400779576
8	88.03256158904405	1.690142639178643	9.326674573766732	0.9506211980105811
9	85.39250542787508	3.1803668026348193	10.169870551902374	1.2572572175877306
10	57.62025538612219	21.412507182911604	13.882692792178197	7.084544638788006
11	51.73267396799606	15.758458851470126	22.576705003835603	9.93216217669821
12	47.10036827460094	18.960055934712642	23.341844316936953	10.597731473749466
13	19.774520266425867	45.13975186048026	23.746635000523682	11.339092872570195
14	13.000999238540603	45.96596361412974	29.080318397595033	11.952718749734622
15	11.244971961876077	20.809850284061156	56.167962657351765	11.777215096711005
16	13.140906787745315	15.281839051827362	54.98479911908489	16.592455041342433
17	13.884532749830866	15.268463975044513	31.72377140366123	39.12323187146339
18	20.463372104543563	20.431526683632022	38.78036283964911	20.32473837217531
19	29.413846105941932	22.340482748273978	28.177111492941638	20.068559652842453
20	32.38198087010182	21.020171597281394	27.56334408057316	19.034503452043626
21	20.696763656024164	27.95589196900945	31.13378190557337	20.21356246939301
22	21.46225680713564	22.099802133784735	28.878206126209772	27.55973493286985
23	17.4897882350277	30.45144068684204	27.198607859732952	24.860163218397314
24	18.547480814903093	21.51073261452321	44.86807503630378	15.073711534269918
25	14.373961485440272	22.895583818563193	42.4332449210092	20.297209774987333
26	14.361647922687808	35.519957879123275	30.42766277256142	19.690731425627497
27	15.81974359482434	34.84101350528917	32.53052206675366	16.808720833132824
28	12.849768873011785	26.706631490450622	45.59025841497556	14.853341221562038
29	13.518664247358245	22.083242514910733	43.97498789874005	20.42310533899097
30	17.193625820550345	30.164053455015864	35.041781192235945	17.600539532197843
31	30.78631297962742	25.09093636860296	26.73897228457634	17.383778367193273
32	30.823536738292916	25.530615479988338	28.369882440859516	15.275965340859232
33	28.239457750326242	27.061177176599138	26.238574570652958	18.460790502421666
34	18.33999020576388	27.34162918342679	29.784102199740143	24.534278411069185
35	18.994236686491032	24.849831148501565	33.83618432412694	22.319747840880463
36	32.77530720216039	22.62235010714215	27.151264333977792	17.45107835671967
37	19.657329117471388	32.04781625333669	30.595240454158162	17.699614175033755
38	19.39400287033394	33.77518265118083	24.411921227166832	22.4188932513184
39	19.484868471334877	32.30470264868981	28.355375082444255	19.85505379753106
40	24.66328774956195	25.780000509526737	25.078056665034293	24.478655075877022
41	16.585095210731765	22.846046497145235	29.304934766424452	31.263923525698544
42	22.802949027511612	24.2777458537262	24.033385323931622	28.885919794830567
43	22.756171642572657	25.994497111266483	24.52578629882611	26.723544947334748
44	19.10980018059892	30.948370788266168	29.11386224095519	20.827966790179723
45	15.91089226623338	39.955529638887086	22.165545236066567	21.968032858812972
46	22.562339180624395	33.76768128536611	25.252074198415368	18.417905335594124
47	21.436780470406404	26.54697978028075	27.65774806167538	24.358491687637464
48	23.703820601298162	22.508626570686925	32.93793115161534	20.849621676399572
49	21.016208611567954	22.013182588905337	33.939646558288445	23.030962241238264
50	19.04143867704214	33.81714783918204	27.0574972612938	20.083916222482017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	639.0
1	1071.5
2	1504.0
3	11044.5
4	20585.0
5	15523.5
6	10462.0
7	11193.5
8	11925.0
9	12784.0
10	13643.0
11	13672.0
12	13701.0
13	12907.0
14	12113.0
15	11178.0
16	10243.0
17	9088.0
18	7933.0
19	7431.0
20	6929.0
21	6578.5
22	6228.0
23	6431.5
24	6635.0
25	7430.0
26	8225.0
27	8289.5
28	8354.0
29	10217.0
30	12080.0
31	14948.0
32	17816.0
33	20831.0
34	23846.0
35	25225.0
36	26604.0
37	31610.0
38	36616.0
39	50879.5
40	65143.0
41	96781.0
42	128419.0
43	150285.0
44	172151.0
45	170263.0
46	168375.0
47	146675.5
48	124976.0
49	119118.5
50	113261.0
51	101156.5
52	89052.0
53	80898.5
54	72745.0
55	62807.0
56	52869.0
57	48748.0
58	44627.0
59	39876.0
60	35125.0
61	32732.5
62	30340.0
63	25828.5
64	21317.0
65	17467.5
66	13618.0
67	11419.0
68	9220.0
69	7690.5
70	6161.0
71	4903.5
72	3646.0
73	3073.5
74	2501.0
75	2201.0
76	1901.0
77	1360.5
78	820.0
79	605.5
80	391.0
81	279.0
82	167.0
83	130.0
84	93.0
85	73.0
86	53.0
87	29.0
88	5.0
89	9.5
90	14.0
91	7.5
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1413076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.758592411184225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.65407631989402	14.323028676655433
2	6.660764579580304	2.3657160663123897
3	2.2292509017873763	1.1876507444132067
4	1.1575375721568577	0.8222495177826155
5	0.7540832885287851	0.6695728882534066
6	0.5415352511806087	0.577014228120282
7	0.42300594105107875	0.5258393066244869
8	0.3320329695466497	0.47171505386032775
9	0.2845985876683046	0.4548663287280092
>10	3.7917461138728736	16.590314370276303
>50	1.7066672669636591	22.234991945651835
>100	1.4598618865984483	38.32344530203984
>500	0.0036294908782834347	0.3531398611613024
>1k	8.0655352850743E-4	0.26390598020267686
>5k	0.0	0.0
>10k+	4.03276764253715E-4	0.8365497299179018
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11681	0.8266363592616393	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2671	0.18902026501051605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02462712550492684	0.0
2	0.0	0.0	0.0	0.08400114360444874	0.0
3	0.0	0.0	0.0	0.12044645864765943	0.0
4	0.0	0.0	0.0	0.1890910326125417	0.0
5	0.0	0.0	0.0	0.32199258921671586	0.0
6	0.0	0.0	0.0	0.4733644899495852	0.0
7	0.0	0.0	0.0	0.5666361894193943	0.0
8	0.0	0.0	0.0	0.829113225332537	0.0
9	0.0	0.0	0.0	0.9452428602566316	0.0
10	0.0	0.0	0.0	1.122232632922787	0.0
11	0.0	0.0	0.0	1.2774259841650413	0.0
12	0.0	0.0	0.0	1.4204473078588837	0.0
13	0.0	0.0	0.0	1.4745845234085073	0.0
14	0.0	0.0	0.0	1.4953194308020235	0.0
15	0.0	0.0	0.0	1.524687985642669	0.0
16	0.0	0.0	0.0	1.592766418791346	0.0
17	0.0	0.0	0.0	1.6844104634145651	0.0
18	0.0	0.0	0.0	1.8323147516481775	0.0
19	0.0	0.0	0.0	1.8919718401558019	0.0
20	0.0	0.0	0.0	1.9582810832538378	0.0
21	0.0	0.0	0.0	2.043768346500825	0.0
22	0.0	0.0	0.0	2.1304586589822487	0.0
23	0.0	0.0	0.0	2.2426253081929066	0.0
24	0.0	0.0	0.0	2.316011311493508	0.0
25	0.0	0.0	0.0	2.3792775477044406	0.0
26	0.0	0.0	0.0	2.44650676962881	0.0
27	0.0	0.0	0.0	2.512179104308615	0.0
28	0.0	0.0	0.0	2.581460586691728	0.0
29	0.0	0.0	0.0	2.655412730808534	0.0
30	0.0	0.0	0.0	2.7653148167543713	0.0
31	0.0	0.0	0.0	2.861912593519386	0.0
32	0.0	0.0	0.0	2.946338342735989	0.0
33	0.0	0.0	0.0	3.03225021159513	0.0
34	0.0	0.0	0.0	3.124106559024426	0.0
35	7.076760202565185E-5	0.0	0.0	3.255026622771882	0.0
36	7.076760202565185E-5	0.0	0.0	3.3555166176483078	0.0
37	7.076760202565185E-5	0.0	0.0	3.4573511969632205	0.0
38	7.076760202565185E-5	0.0	0.0	3.5514721076573377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	30	2.5281315E-6	44.0	26
CGAGTTA	25	4.443362E-5	44.0	32
GTCGACC	30	2.5281315E-6	44.0	15
GTTTCGA	30	2.5281315E-6	44.0	10
CGCATGG	110	0.0	44.0	2
CCGTCGA	20	7.857096E-4	44.0	20
ATTTTCG	30	2.5281315E-6	44.0	1
TCAGCGA	20	7.857096E-4	44.0	21
TACGATG	70	0.0	44.0	1
ATATACG	50	2.7284841E-11	44.0	1
CGGATAT	20	7.857096E-4	44.0	13
TCCGTCG	20	7.857096E-4	44.0	19
CCGGTGA	45	4.802132E-10	44.0	29
CCGCGAA	20	7.857096E-4	44.0	26
TTGCACG	25	4.443362E-5	44.0	1
CACGTAT	20	7.857096E-4	44.0	24
ATACGAG	70	0.0	44.0	1
CGGTAAC	20	7.857096E-4	44.0	20
TCGACAT	20	7.857096E-4	44.0	19
GTGCGTA	20	7.857096E-4	44.0	42
>>END_MODULE