FastQCFastQC Report
Sat 18 Jun 2016
SRR3549137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250997
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215560.6630581326282369No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA64120.19723180304380472No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA53680.1651185774702345No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA41460.12753010845595983No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA32540.10009237166321594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA254.4446424E-544.033
GTCGACG351.4471698E-744.01
CTATCGT302.529152E-644.013
CGCCTAT254.4446424E-544.034
CGAATCG207.858609E-444.037
CGGTCTA4650.041.63440731
TACGGGA9900.041.5555574
TCGAACG800.041.251
TACGTAG1450.040.965521
ACGTTAG1350.040.740741
CGTTTTT124650.040.452471
CGACGGT4800.039.87528
GGCGATA12150.039.8353928
CGTTAGG6200.039.7419322
TATTACG501.3496901E-939.6000021
CGATACG1000.039.60000210
GCGTTAG3650.039.178081
CGTTACT1350.039.1111129
CGTAACG452.3532266E-839.111112
CGCGTCA900.039.1111139
Produced by FastQC (version 0.11.5)