##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3250997
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70082316286358	31.0	31.0	33.0	30.0	34.0
2	32.12615576083275	33.0	31.0	34.0	30.0	34.0
3	32.196189353604446	33.0	31.0	34.0	30.0	34.0
4	35.884033113534095	37.0	35.0	37.0	35.0	37.0
5	35.86870335469396	37.0	35.0	37.0	35.0	37.0
6	35.91964465054874	37.0	35.0	37.0	35.0	37.0
7	36.097593138351094	37.0	35.0	37.0	35.0	37.0
8	36.046859163511996	37.0	35.0	37.0	35.0	37.0
9	37.80277373371923	39.0	38.0	39.0	35.0	39.0
10	37.548329020297466	39.0	37.0	39.0	35.0	39.0
11	37.29939707726584	39.0	37.0	39.0	34.0	39.0
12	36.706778566698155	39.0	35.0	39.0	33.0	39.0
13	36.533822085963166	39.0	35.0	39.0	33.0	39.0
14	37.60691289472122	40.0	35.0	41.0	33.0	41.0
15	37.74381366700738	40.0	35.0	41.0	33.0	41.0
16	37.7969721288577	40.0	35.0	41.0	33.0	41.0
17	37.73141439379981	40.0	35.0	41.0	33.0	41.0
18	37.65426390734904	39.0	36.0	41.0	33.0	41.0
19	37.59666803752818	39.0	36.0	41.0	33.0	41.0
20	37.449579313669005	39.0	35.0	41.0	33.0	41.0
21	37.33676038458356	39.0	35.0	41.0	33.0	41.0
22	37.327893873787026	39.0	35.0	41.0	33.0	41.0
23	37.31455673444177	39.0	35.0	41.0	33.0	41.0
24	37.27763975174385	39.0	35.0	41.0	33.0	41.0
25	37.18593619126686	39.0	35.0	41.0	33.0	41.0
26	37.09334828669482	39.0	35.0	41.0	33.0	41.0
27	37.00894494827279	39.0	35.0	41.0	33.0	41.0
28	36.981159318203	39.0	35.0	41.0	32.0	41.0
29	36.91375076630338	38.0	35.0	41.0	32.0	41.0
30	36.81535633530268	38.0	35.0	41.0	32.0	41.0
31	36.68391388857018	38.0	35.0	41.0	32.0	41.0
32	36.52967043648456	38.0	35.0	41.0	31.0	41.0
33	36.379438676812065	38.0	35.0	41.0	31.0	41.0
34	36.224668001846815	38.0	35.0	41.0	30.0	41.0
35	36.0453260953486	38.0	35.0	41.0	30.0	41.0
36	35.886623395838264	38.0	35.0	41.0	30.0	41.0
37	35.845594136198834	38.0	35.0	41.0	29.0	41.0
38	35.76736767213258	38.0	35.0	41.0	29.0	41.0
39	35.71800066256598	38.0	35.0	41.0	29.0	41.0
40	35.579498842970324	38.0	35.0	41.0	28.0	41.0
41	35.49121115768486	38.0	35.0	40.0	28.0	41.0
42	35.46207025106452	38.0	35.0	40.0	28.0	41.0
43	35.38463585170949	38.0	35.0	40.0	27.0	41.0
44	35.25784889989133	38.0	34.0	40.0	27.0	41.0
45	35.16177283461043	37.0	34.0	40.0	27.0	41.0
46	35.110650978761285	37.0	34.0	40.0	27.0	41.0
47	35.04611939045161	37.0	34.0	40.0	27.0	41.0
48	34.96261546842399	37.0	34.0	40.0	26.0	41.0
49	34.89632811103794	37.0	34.0	40.0	26.0	41.0
50	34.73501974932613	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	7.0
12	12.0
13	16.0
14	24.0
15	71.0
16	177.0
17	395.0
18	964.0
19	1922.0
20	3633.0
21	5674.0
22	9005.0
23	13418.0
24	19434.0
25	28681.0
26	40823.0
27	49890.0
28	53921.0
29	57158.0
30	64125.0
31	76402.0
32	94748.0
33	125501.0
34	237365.0
35	474351.0
36	192306.0
37	247959.0
38	429264.0
39	1023750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.81264270622212	22.99906767062535	29.141244978079033	14.047044645073495
2	31.717500815903556	26.05899051890851	29.760132045646305	12.463376619541636
3	29.666037833932172	24.44733108028091	32.87932901814428	13.007302067642634
4	26.425247393338104	26.48756673721938	32.41528675664726	14.671899112795245
5	23.353328225156776	32.38867953430901	30.13143352639206	14.126558714142154
6	21.575412096658347	41.352914198321315	27.256407803513817	9.815265901506523
7	86.26682829913408	4.594313682848677	7.430920422258157	1.7079375957590854
8	87.1529257024845	3.73599237403172	7.152544281031327	1.9585376424524539
9	81.96064161240383	6.4001597048536185	8.760758622662525	2.8784400600800306
10	45.35771641745594	29.493629185139202	14.632034418979778	10.51661997842508
11	37.99763580218622	22.539731657703776	25.90519154585501	13.557440994254993
12	35.06702713044644	22.73871676904039	27.516727945304165	14.677528155209002
13	21.213400073885026	35.99769547618777	27.004946482571345	15.78395796735586
14	16.059842565219224	37.329440783858	30.160870649834497	16.449846001088282
15	16.19069473149314	24.841517848217023	43.52836991236842	15.439417507921416
16	17.970641006435873	21.5833173638733	41.367740419323674	19.078301210367158
17	17.7602747710933	22.10887921459171	30.35539559095256	29.775450423362432
18	20.550065103105293	24.536842082598046	34.74254205709818	20.170550757198484
19	27.118542404068656	24.668155645791124	27.933215564333036	20.280086385807184
20	28.475356944346615	23.96929311223603	27.92958590856897	19.625764034848387
21	22.009155960463822	28.89956527182277	29.377633999662255	19.713644768051157
22	21.809770971797267	25.249023607219566	27.686522011555226	25.254683409427937
23	19.606846761162807	29.100611289398298	28.781324621339238	22.511217328099654
24	18.33422177873434	25.091841056758895	38.82839633503199	17.745540829474773
25	17.841572908249375	27.03459892457606	35.05066291971355	20.07316524746101
26	17.01665673637964	33.70319935699725	28.40156419707554	20.878579709547562
27	18.1869131223437	32.859396671236546	29.381909611113144	19.57178059530661
28	15.93437336300218	29.208824246838738	36.418089589132194	18.438712801026885
29	16.53911092504853	25.675908036826854	36.0972649313426	21.687716106782013
30	17.972517353907126	31.64521529856841	31.329742845041075	19.052524502483394
31	26.550316718225208	27.2914432095754	26.882768578377647	19.27547149382174
32	26.505715016039694	26.978954456125305	28.441244332123343	18.074086195711654
33	24.702575855960497	27.98621469044727	27.83487650096263	19.47633295262961
34	18.71370536484654	28.20525518787006	29.418759845056762	23.66227960222664
35	20.840806681765624	25.474000745002222	31.79000165180097	21.89519092143118
36	25.816234219840865	27.71577457623	27.37314122406142	19.094849979867714
37	19.50681590908881	33.19323272214646	29.274373369154137	18.025577999610583
38	19.501709783183436	32.01965427836445	27.454316322039052	21.024319616413056
39	20.44345165498461	31.414301520425887	28.095504240699082	20.04674258389042
40	22.91026414358426	27.76978877556639	27.79181278850765	21.5281342923417
41	17.950093463635923	26.431307072876415	29.032939741254758	26.5856597222329
42	20.2349925269079	27.220049726283968	26.28507500929715	26.259882737510985
43	20.652649018131978	27.651640404466693	27.676863436047466	24.01884714135387
44	18.629269728640168	30.005133809720526	29.219467135774043	22.146129325865267
45	17.48448245261377	34.98363732725684	25.863019867443743	21.668860352685655
46	20.02127347395276	33.10003054447605	26.917004229779355	19.961691751791836
47	21.16824469539652	28.1898445307701	28.30765454412908	22.3342562297043
48	20.495281908903635	26.225985443849993	32.094985015366056	21.183747631880312
49	20.032531558780274	26.79685647202997	32.374283950431206	20.796328018758555
50	19.289405680780387	31.506919261998707	29.429402733992067	19.774272323228843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3326.0
1	6220.5
2	9115.0
3	24460.5
4	39806.0
5	28190.5
6	16575.0
7	16024.5
8	15474.0
9	15499.5
10	15525.0
11	14871.0
12	14217.0
13	13615.5
14	13014.0
15	12484.0
16	11954.0
17	11841.0
18	11728.0
19	11276.0
20	10824.0
21	12113.5
22	13403.0
23	15553.5
24	17704.0
25	23845.5
26	29987.0
27	39854.0
28	49721.0
29	57825.5
30	65930.0
31	70440.5
32	74951.0
33	88235.0
34	101519.0
35	108667.5
36	115816.0
37	122122.5
38	128429.0
39	145326.0
40	162223.0
41	208604.0
42	254985.0
43	282491.5
44	309998.0
45	320921.5
46	331845.0
47	311713.0
48	291581.0
49	273715.5
50	255850.0
51	233060.5
52	210271.0
53	192774.5
54	175278.0
55	156862.5
56	138447.0
57	123755.0
58	109063.0
59	101146.0
60	93229.0
61	78380.5
62	63532.0
63	51024.5
64	38517.0
65	29971.0
66	21425.0
67	17260.5
68	13096.0
69	10854.0
70	8612.0
71	7400.5
72	6189.0
73	4867.0
74	3545.0
75	2654.0
76	1763.0
77	1458.0
78	1153.0
79	904.0
80	655.0
81	492.5
82	330.0
83	262.5
84	195.0
85	156.5
86	118.0
87	80.5
88	43.0
89	26.5
90	10.0
91	9.5
92	9.0
93	8.5
94	8.0
95	6.0
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3250997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.794396446736588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5742228352685	11.624582031191851
2	7.5138850194351265	2.2232678766543663
3	2.5947477263577086	1.1516317962901295
4	1.3304830827623113	0.787347767682475
5	0.8315374231469084	0.615104714916656
6	0.6175054241609387	0.5481372031828297
7	0.47872805590802836	0.4957744854495194
8	0.3864875332532652	0.45742798309360766
9	0.3335193545325281	0.4440795798252523
>10	4.061864205528896	14.243692281184261
>50	1.3453424746730875	14.383109177660849
>100	1.910790453977566	49.484122142083855
>500	0.017510829420168204	1.6669869209106598
>1k	0.0027426600296649	0.8342425558362063
>5k	4.2194769687152304E-4	0.36768098278024963
>10k+	2.1097384843576152E-4	0.6728125012573057
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21556	0.6630581326282369	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6412	0.19723180304380472	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	5368	0.1651185774702345	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4146	0.12753010845595983	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3254	0.10009237166321594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049461749734004676	0.0
2	0.0	0.0	0.0	0.165887572335502	0.0
3	0.0	0.0	0.0	0.23115985649940618	0.0
4	0.0	0.0	0.0	0.3367582313979373	0.0
5	0.0	0.0	0.0	0.5734856107218801	0.0
6	0.0	0.0	0.0	0.8094747549751661	0.0
7	3.0759794610699425E-5	0.0	0.0	0.9653653940621908	0.0
8	3.0759794610699425E-5	0.0	0.0	1.320548742431937	0.0
9	3.0759794610699425E-5	0.0	0.0	1.464658380183064	0.0
10	3.0759794610699425E-5	0.0	0.0	1.7086450710351317	0.0
11	3.0759794610699425E-5	0.0	0.0	1.9299002736698927	0.0
12	3.0759794610699425E-5	0.0	0.0	2.1325458005651803	0.0
13	3.0759794610699425E-5	0.0	0.0	2.225194301932607	0.0
14	3.0759794610699425E-5	0.0	0.0	2.2621060554654466	0.0
15	3.0759794610699425E-5	0.0	0.0	2.3066154782671284	0.0
16	3.0759794610699425E-5	0.0	0.0	2.403908708620771	0.0
17	3.0759794610699425E-5	0.0	0.0	2.5212880848552	0.0
18	3.0759794610699425E-5	0.0	0.0	2.6953885223517586	0.0
19	3.0759794610699425E-5	0.0	0.0	2.7702271026395904	0.0
20	3.0759794610699425E-5	0.0	0.0	2.848110902593881	0.0
21	3.0759794610699425E-5	0.0	0.0	2.951094694950503	0.0
22	3.0759794610699425E-5	0.0	0.0	3.0568468688220873	0.0
23	6.151958922139885E-5	0.0	0.0	3.180931880281649	0.0
24	6.151958922139885E-5	0.0	0.0	3.2764718023424813	0.0
25	6.151958922139885E-5	0.0	0.0	3.3570624642225138	0.0
26	6.151958922139885E-5	0.0	0.0	3.437837684870211	0.0
27	6.151958922139885E-5	0.0	0.0	3.51698263640354	0.0
28	6.151958922139885E-5	0.0	0.0	3.604801850017087	0.0
29	6.151958922139885E-5	0.0	0.0	3.7016029236569583	0.0
30	6.151958922139885E-5	0.0	0.0	3.8317476146548275	0.0
31	6.151958922139885E-5	0.0	0.0	3.9419599587449636	0.0
32	6.151958922139885E-5	0.0	0.0	4.047742892411159	0.0
33	6.151958922139885E-5	0.0	0.0	4.151218841481552	0.0
34	6.151958922139885E-5	0.0	0.0	4.25933951953816	0.0
35	6.151958922139885E-5	0.0	0.0	4.399788741730614	0.0
36	6.151958922139885E-5	0.0	0.0	4.519844220096173	0.0
37	6.151958922139885E-5	0.0	0.0	4.639038424212634	0.0
38	6.151958922139885E-5	0.0	0.0	4.752941943656054	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTA	25	4.4446424E-5	44.0	33
GTCGACG	35	1.4471698E-7	44.0	1
CTATCGT	30	2.529152E-6	44.0	13
CGCCTAT	25	4.4446424E-5	44.0	34
CGAATCG	20	7.858609E-4	44.0	37
CGGTCTA	465	0.0	41.634407	31
TACGGGA	990	0.0	41.555557	4
TCGAACG	80	0.0	41.25	1
TACGTAG	145	0.0	40.96552	1
ACGTTAG	135	0.0	40.74074	1
CGTTTTT	12465	0.0	40.45247	1
CGACGGT	480	0.0	39.875	28
GGCGATA	1215	0.0	39.835392	8
CGTTAGG	620	0.0	39.741932	2
TATTACG	50	1.3496901E-9	39.600002	1
CGATACG	100	0.0	39.600002	10
GCGTTAG	365	0.0	39.17808	1
CGTTACT	135	0.0	39.11111	29
CGTAACG	45	2.3532266E-8	39.11111	2
CGCGTCA	90	0.0	39.11111	39
>>END_MODULE