FastQCFastQC Report
Sat 18 Jun 2016
SRR3549136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880027
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102640.5459496060428919No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36800.1957418696646378No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23710.12611521004751527No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA23200.12340248304944558No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21050.11196647707719093No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20380.10840269847188365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA1450.044.00000410
TGATCAC254.4439228E-544.035
AACCGTG351.4467332E-744.038
AACCGTA207.857761E-444.03
CTATGCG551.8189894E-1244.01
ATAGGCG207.857761E-444.02
ACGTTAC207.857761E-444.01
ACCATAC207.857761E-444.033
GACGTAA351.4467332E-744.043
CGCATCG600.044.021
GATCACG302.528579E-644.01
CGATTCG502.7284841E-1144.010
TCTAGCG502.7284841E-1144.01
CCTCGAG302.528579E-644.01
ATACTAC254.4439228E-544.039
CCGTAAA207.857761E-444.043
TACGCAC207.857761E-444.020
TGCGCAA207.857761E-444.012
GTTCGGA207.857761E-444.042
TATTCGC254.4439228E-544.038
Produced by FastQC (version 0.11.5)